Diagnostic value of impulse oscillometry in chronic obstructive pulmonary disease: a multicentre, retrospective, observational study by Hui Zhou, Hao Wang, Lin Liu, Lei Chen, Tao Wang, Yongchun Shen, Lijuan Gao, Dan Xu, Fu-Qiang Wen, Yanqiu Wu, Shuguang Xiong, Rong Qiu, Huizhuo Jia, Jiangyue Qin

“…Receiver operating characteristic curve analysis was used to evaluate the IOS performance in COPD diagnosis and severity staging.Results Patients with COPD exhibited significant increases in Z5, R5, R20, R5−R20, Fres and Rp, but a decrease in X5 compared with non-COPD subjects (p<0.0001). …”
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Perioperative sildenafil citrate administration in hepatectomy: a study protocol of randomized controlled trial by Qisheng Chen, Shixuan Peng, Yingying Zhou, Shuzhai Li, Feng Xu, Yi Yu, Pan Wang, Xinran Peng, Chunbo Zhao, Aowen Xie, Zhiming Zhang

“…Discussion This trial evaluated the influence of administration of sildenafil citrate combined with IPM on IBL and surgical results during open or laparoscopic hepatectomy, as well as its safety and feasibility. …”
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Comparison of the STANDARD M10 C. difficile, Xpert C. difficile, and BD MAX Cdiff assays as confirmatory tests in a two-step algorithm for diagnosing Clostridioides difficile infec... by Hyunseul Choi, Minhee Kang, Sun Ae Yun, Hui-Jin Yu, Eunsang Suh, Tae Yeul Kim, Hee Jae Huh, Nam Yong Lee

“…In conclusion, the M10 assay is a reliable diagnostic tool, performing comparably to the Xpert and BD MAX assays when used as confirmatory testing in the two-step algorithm.IMPORTANCEWhile numerous studies have assessed nucleic acid amplification tests (NAATs) as stand-alone tests for diagnosing Clostridioides difficile infection, limited research has compared their performance as confirmatory tests in a two-step algorithm. This study evaluated the performance of three commercial NAATs (M10, Xpert, and BD MAX assays) using 200 archived stool specimens initially tested as glutamate dehydrogenase (GDH)-positive but toxin-negative by GDH/toxin A/B enzyme immunoassay, the first step in the two-step algorithm. …”
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Persistent lineage plasticity driving lung cancer development and progression by Fanchen Meng, Jianyu Li, Zhijun Xia, Qinglin Wang, Qinhong Sun, Siwei Wang, Lin Xu, Rong Yin

“…Certain driver mutations have lineage‐restricted tumorigenic potential, requiring lineage reprogramming for tumor initiation. Lineage transitions in lung cancer require specific genomic and epigenetic alterations. …”
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Monoclonal antibodies in severe asthma: outcomes from real-world data by Silvia del-Barrio-Buesa, Silvia del-Barrio-Buesa, Álvaro Narrillos-Moraza, Álvaro Narrillos-Moraza, Julia García-de-Pedro, Julia García-de-Pedro, Julia García-de-Pedro, Francisco Javier de-Castro-Martínez, Francisco Javier de-Castro-Martínez, María Esther Durán-García, María Esther Durán-García, Vicente Escudero-Vilaplana, Vicente Escudero-Vilaplana, Elena Lobato-Matilla, Elena Lobato-Matilla, Rosa Romero-Jimenez, Rosa Romero-Jimenez, Esther Chamorro-de-Vega, Esther Chamorro-de-Vega, Paula Ruiz-Briones, Paula Ruiz-Briones, Félix Jesús Garcia-Moreno, Félix Jesús Garcia-Moreno, María Martín-Bartolomé, María Martín-Bartolomé, Ana Herranz, Ana Herranz, María Sanjurjo, María Sanjurjo

“…Monoclonal antibodies targeting type 2 (T2) inflammation have become key therapeutic options, but their real-world performance remains insufficiently characterized.ObjectiveTo evaluate the real-world effectiveness, adherence, and persistence of benralizumab, mepolizumab, omalizumab, and reslizumab in adults with uncontrolled severe asthma after 12 months of treatment.MethodsA retrospective real-world observational study was conducted in patients with uncontrolled severe asthma who initiated treatment with benralizumab, mepolizumab, omalizumab, and reslizumab between January 2015 and December 2022. …”
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Supporting primary care clinicians in caring for patients with alcohol use disorder: study protocol for Records for Alcohol Care Enhancement (RACE), a factorial four-arm randomized... by Kara M. Magane, Richard Saitz, Sarah Fielman, Marc R. LaRochelle, Christopher W. Shanahan, Christine A. Pace, Michael LaValley, Kaley Penington, Skylar Karzhevsky, Emily Hurstak

“…Discussion The study will evaluate how a targeted EHR innovation alone, compared with population health and care management enhancements alone or in combination, impact engagement in AUD treatment, a national quality of care measure. …”
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Data extraction from free-text stroke CT reports using GPT-4o and Llama-3.3-70B: the impact of annotation guidelines by Jonas Wihl, Enrike Rosenkranz, Severin Schramm, Cornelius Berberich, Michael Griessmair, Piotr Woźnicki, Francisco Pinto, Sebastian Ziegelmayer, Lisa C. Adams, Keno K. Bressem, Jan S. Kirschke, Claus Zimmer, Benedikt Wiestler, Dennis Hedderich, Su Hwan Kim

“…Abstract Background To evaluate the impact of an annotation guideline on the performance of large language models (LLMs) in extracting data from stroke computed tomography (CT) reports. …”
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β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis by Yijia Zhang, Xinya Zhu, Liyuan Chen, Tianyu Gao, Guang Chen, Jin Zhu, Guoyu Wang, Daiying Zuo

“…Furthermore, the functional impact of TAM-derived OSM on PDAC progression was evaluated through in vitro co-culture systems and an in vivo Panc02 lung metastasis model. …”
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Impact of COVID-19 pandemic on non-communicable diseases care and service deliveries in Sub-Saharan Africa: A systematic review by Yordanos Tadesse Kidanemariam, Finina Abebe, Eshetu Girma, Adamu Addisse, Zewdie Birhanu, Kalkidan Hassen, Abonesh Taye, Demuma Amdisa, Eyasu Alem Lake, Mistire Teshome Guta, Sudhakar Morankar, Kiya Kedir, Firaol Mesfin, Fiseha Wadilo, Elifaged Hailemeskel, Melat Dereje, Eskedar Hailegebrel, Rawleigh Howe, Minyahil Tadesse Boltena, Hailemicheal Getachew, Endalkachew Worku Mengesha, Tadesse Dagget Tesfaye, Netsanet Fentahun, Bereket Damtew, Adisalem Debebe, Zerihun Tariku, Addisu worku, Yoseph Gebreyohannes Abraha, Sabit Ababor Ababulgu

“…Result A total of 2,387 records were initially identified, with 2,207articles excluded during abstract and title screening, and 60 articles excluded during full-text screening. …”
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Optimization of the Preparation Process of 6'-O-Caffeoylarbutin Liposomes and Its Activity by Mengzhe LIU, Kangjia HAN, Wenbo WANG, Yang YANG, Sida XIE, Ping ZHAO, Junming XU, Yuanping HE, Xiaoqin YANG

“…Objective: To optimize the preparation process of 6'-O-caffeoylarbutin liposomes using the Box-Behnken response surface methodology, and to characterize their structure and evaluate their activity. Methods: The ethanol injection method was used. …”
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Exome sequencing reveals a rare damaging variant in GRIN2C in familial late-onset Alzheimer's disease by Elisa Rubino, Maria Italia, Elisa Giorgio, Silvia Boschi, Paola Dimartino, Tommaso Pippucci, Fausto Roveta, Clara Maria Cambria, Gabriella Elia, Andrea Marcinnò, Salvatore Gallone, Ekaterina Rogaeva, Flavia Antonucci, Alfredo Brusco, Fabrizio Gardoni, Innocenzo Rainero

“…Genomic DNA from family members was extracted and initially screened for pathogenic mutations in APP, PSEN1, and PSEN2, and screened for 77 genes associated with neurodegenerative conditions using NeuroX array assay. …”
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Hyaluronic acid modified chuanxiong oil liposomes as a novel therapeutic agent for photoaging prevention by Xiaofang He, Yue Hu, Yuanyuan Wu, Yuting Luo, Huiyi Feng, Qianqian Wu, Haolin Liu, Leying Gao, Haofeng Yang, Yu Long, Yin Ma, Xiaoqiu Li, Jie Deng, Yuntong Ma, Nan Li

“…In this study, a composite nano-system (HA-CXO-Lip) comprising chuanxiong oil (CXO) and hyaluronic acid (HA) was initially fabricated. Subsequently, both in vitro HaCaT cell models and in vivo murine photoaging models were established to systematically evaluate the therapeutic efficacy and mechanistic actions of HA-CXO-Lip against photoaging under controlled experimental conditions. …”
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Centre-level fluid management practices in the BISTRO trial and their lack of association with participant fluid status and blood pressure in non-anuric haemodialysis patients by Neena Johal, Radha Sharma, John Belcher, David Coyle, Elizabeth J. Lindley, David Keane, Fergus J. Caskey, Indranil Dasgupta, Andrew Davenport, Ken Farrington, Sandip Mitra, Paula Ormandy, Martin Wilkie, Jamie Macdonald, Ivonne Solis-Trapala, Julius Sim, Simon J. Davies

“…Survey responses revealed substantial scope for developing and evaluating standardised protocols to optimise fluid management.…”
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PARP inhibitors enhance antitumor immune responses by triggering pyroptosis via TNF–caspase 8–GSDMD/E axis in ovarian cancer by Ping Jin, Qi Zhang, Xin Li, Yu Xia, Yong Fang, Xiong Li, Ning Jin, Xiang-Ping Yang, Qing-Lei Gao, Zhengmao Zhang, Pu Huang, Yi-yu Qian, Zanhong Wang, Wen Pan, Si-Yuan Wang, Emmanuel Kwateng Drokow, Pingfei Li, Zhiqiang Han

“…On PARP inhibition, the NF-κB signaling pathway was activated, leading to increased secretion of TNF-α and subsequent initiation of the TNFR1–caspase 8 cascade. Impeding pyroptosis through the depletion of Gsdme significantly compromised the tumor-suppressing effects of PARP inhibition and undermined the anti-immune response in the syngeneic ID8 mouse model.Conclusions PARPis induce a specific type of ICD called pyroptosis via TNF–caspase 8–GSDMD/E axis, resulting in an inflamed TIME and augmentation of tumor-targeting immune responses. …”
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Risk factors and predictive model for renal outcomes in autoimmune membranous nephropathy with and without acute kidney injury: a retrospective cohort study by Zhenzhou Li, Liyan Yang, Linxia Wei, Mengjie Weng, Jiaqun Lin, Yi Chen, Binbin Fu, Guifen Li, Caiming Chen, Yanfang Xu, Jianxin Wan, Jiong Cui

“…Model performance was evaluated using the C-index, time-dependent receiver operating characteristic (Time-ROC) curves, calibration curves, and decision curve analysis (DCA). …”
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Combination of spatial transcriptomics analysis and retrospective study reveals liver infection of SARS-COV-2 is associated with clinical outcomes of COVID-19Research in context by Shiqi Chen, Yi Zhang, Asha Ashuo, Shu Song, Lunzhi Yuan, Weixia Wang, Cong Wang, Zunguo Du, Yangtao Wu, Dan Tan, Chenlu Huang, Jingna Chen, Yaming Li, Jinjin Bai, Huilin Guo, Zehong Huang, Yi Guan, Ningshao Xia, Zhenghong Yuan, Jiming Zhang, Quan Yuan, Zhong Fang

“…Findings: SARS-CoV-2 proteins were initially detected in the livers of naturally infected golden (Syrian) hamsters, prompting us to investigate the situation in clinical cases. …”
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Development and validation of an integrative 54 biomarker-based risk identification model for multi-cancer in 42,666 individuals: a population-based prospective study to guide adva... by Renjia Zhao, Huangbo Yuan, Yanfeng Jiang, Zhenqiu Liu, Ruilin Chen, Shuo Wang, Linyao Lu, Ziyu Yuan, Zhixi Su, Qiye He, Kelin Xu, Tiejun Zhang, Li Jin, Ming Lu, Weimin Ye, Rui Liu, Chen Suo, Xingdong Chen

“…The model was trained and internally validated in the discovery cohort, externally applied in the validation cohort, and further evaluated through a prospective clinical follow-up to assess cancer events via clinical examinations. …”
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Long-term outcome of a treat-to-target strategy in late-onset rheumatoid arthritis with chronic lung disease: 5-year results of a prospective observational study by Manami Nomura, Takahiko Sugihara, Hiroyuki Baba, Tadashi Hosoya, Mari Kamiya, Tatsuro Ishizaki, Takumi Matsumoto, Kanae Kubo, Fumio Hirano, Masayo Kojima, Nobuyuki Miyasaka, Shinsuke Yasuda, Masayoshi Harigai

“…Abstract Background Controlling disease activity and improving physical function would be more difficult in patients with late-onset rheumatoid arthritis (LORA) who have chronic lung disease (CLD) at baseline. Our aim was to evaluate 5-year outcomes of following a treat-to-target (T2T) strategy targeting low disease activity (LDA) in LORA with CLD. …”
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Crocin promotes ferroptosis in gastric cancer via the Nrf2/GGTLC2 pathway by Nan Yan, Gaofu Li, Linglin Zhao, Qijing Guo, Jie Yang, Jianhong Liu, Wei Zhou, Yue Gao, Yue Gao, Yushuang Luo, Yushuang Luo

“…The UCSC and JASPAR databases predicted Nrf2 binding sites in the GGTLC2 promoter. Molecular docking evaluated crocin’s affinity for Nrf2 and GGTLC2. Immunofluorescence and nuclear-cytoplasmic fractionation assays analyzed Nrf2 expression and localization. …”
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Trends of breastfeeding indicators in Brazil from 1996 to 2019 and the gaps to achieve the WHO/UNICEF 2030 targets by Gilberto Kac, Neilane Bertoni, Cristiano Siqueira Boccolini, Elisa Maria de Aquino Lacerda, Natália Oliveira, Nadya Helena Alves-Santos, Dayana Rodrigues Farias, Sandra Patricia Crispim, Leticia Barroso Vertulli Carneiro, Raquel Machado Schincaglia, Elsa Regina Justo Giugliani, Inês Rugani Ribeiro de Castro

“…The indicators of early initiation of breastfeeding (EIBF), exclusive breastfeeding of infants 0–5 months of age (EBF<6 mo), continued breastfeeding at 1 year of age (CBF1yr) and CBF at 2 years of age (CBF2yr) were analysed using prevalence and 95% CI. …”
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