Multi-omics integration reveals functional signatures of gut microbiome in atherosclerosis by Hongbo Shi, Meitao Wu, Xiaoliang Wu, Zhuoxin Liu, Shuo Jiang, Gen Li, Yetong Yang, Yanghe Fu, Qiuping Wang, Guangde Zhang, Liang Cheng

“…While the associations between microbial imbalance and AS pathogenesis are well-documented, the pathophysiological mechanisms governing microbe-host crosstalk remain incompletely characterized. Current research limitations stem from methodological heterogeneity across studies and the absence of consensus regarding disease-specific microbial signatures. …”
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The Current Status of Somatostatin-Interneurons in Inhibitory Control of Brain Function and Plasticity by Isabelle Scheyltjens, Lutgarde Arckens

“…This review informs on those SST-subpopulations hitherto described based on anatomical, molecular, or electrophysiological characteristics and whose functional roles can be attributed based on specific cortical wiring patterns. …”
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Ub-POD: A Ubiquitin-Specific Proximity-Dependent Labeling Technique to Identify E3 Ubiquitin Ligase Substrates in Human Cells by Urbi Mukhopadhyay, Sophie Levantovsky, Christian Behrends, Sagar Bhogaraju

“…E3 ligases share a dual function of 1) transferring covalently attached ubiquitin from the catalytic cysteine of E2 (E2~Ub) to the substrate and 2) providing substrate specificity. …”
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Effective Seed Scheduling for Directed Fuzzing with Function Call Sequence Complexity Estimation by Xi Peng, Peng Jia, Ximing Fan, Cheng Huang, Jiayong Liu

“…Directed grey-box fuzzers focus on testing specific target code. They have been utilized in various security applications, such as reproducing known crashes and identifying vulnerabilities resulting from incomplete patches. …”
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Review and Validation of Plant Gene Function Research Methods Bypassing Tissue Culture by Huanghuang Jiang, Suya Huang, Jiayi Wang, Deming Sun, Min Wang, Su Lin, Jinhua Liu, Kai Xu, Bei Liu, Haijie Ma

“…However, the genetic transformation systems for most horticultural plants remain incomplete. Genome editing has significantly contributed to the improvement of crop traits, but it heavily relies on effective genetic transformation. …”
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The Role of microRNA in the Regulation of Differentiation and the Functionality of Osteoblasts, Osteoclasts, and Their Precursors in Osteoporosis by Bulat I. Yalaev, Elena I. Kaletnik, Yulia S. Karpova, Zhanna E. Belaya, Ildar R. Minniakhmetov, Natalia G. Mokrysheva, Rita I. Khusainova

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Different Long-Term Nutritional Regimens of <i>Drosophila melanogaster</i> Shape Its Microbiota and Associated Metabolic Activity in a Sex-Specific Manner by Repac Jelena, Trajković Jelena, Rakić Marija, Lunić Tanja, Savić Tatjana, Božić Bojan, Božić Nedeljković Biljana, Sofija Pavković-Lučić

“…The dietary habits of fruit flies profoundly influence their fitness, morphology, and physiology yet the mechanisms underlying these effects remain incompletely understood. To address this gap, the relationship between dietary regimens and the composition and function of adult <i>Drosophila melanogaster</i> microbiota was investigated in the present study. …”
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Genetic analysis and functional assessment of a TGFBR2 variant in micrognathia and cleft palate. by Jes-Rite Michaels, Paul P R Iyyanar, Ammar Husami, Andrew M Vontell, Samantha A Brugmann, Rolf W Stottmann

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Functional thalamocortical innervation of VIP- and SST-expressing GABAergic interneurons in mouse barrel cortex by Michael Feyerabend, Mirko Witte, Martin Möck, Jochen F. Staiger

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DNA G-quadruplex structures act as functional elements in α- and β-globin enhancers by Colm Doyle, Krzysztof Herka, Sean M. Flynn, Larry Melidis, Somdutta Dhir, Stefan Schoenfelder, David Tannahill, Shankar Balasubramanian

“…Abstract Background Enhancer elements interact with target genes at a distance to modulate their expression, but the molecular details of enhancer–promoter interaction are incompletely understood. G-quadruplex DNA secondary structures (G4s) have recently been shown to co-occur with 3D chromatin interactions; however, the functional importance of G4s within enhancers remains unclear. …”
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Klf9 Loss of Function Protects Against Glucocorticoids Induced Skeletal Muscle Wasting by Yujie Zhang, Jingran Hao, Yueyao Feng, Tongtong Qiu, Jinjin Wu, Xuenan Zhou, Heng Fan, Yongsheng Chang

“…We performed Klf9 gain‐ or loss‐of‐function studies in differentiated C2C12 myotubes using lentiviruses encoding Klf9 or the shRNA specific to Klf9 in vitro. …”
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In-depth functional analysis of BRD9 in fetal hematopoiesis reveals context-dependent roles by Yifan Zhang, Masaki Nomura, Koutarou Nishimura, Weijia Zang, Yui Koike, Muran Xiao, Hiromi Ito, Miki Fukumoto, Atsushi Tanaka, Yumi Aoyama, Wataru Saika, Chihiro Hasegawa, Hiromi Yamazaki, Akifumi Takaori-Kondo, Daichi Inoue

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The APOE–Microglia Axis in Alzheimer’s Disease: Functional Divergence and Therapeutic Perspectives—A Narrative Review by Aiwei Liu, Tingxu Wang, Liu Yang, Yu Zhou

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Intraperitoneal injection of cell-engineered pancreas in rats with experimental type i diabetes (preliminary results) by A. S. Ponomareva, N. V. Baranova, A. O. Nikolskaya, L. A. Kirsanova, N. A. Onishchenko, Z. Z. Gonikova, G. N. Bubentsova, Е. A. Volkova, Yu. B. Basok, V. I. Sevastianov

“…The viability and functional activity of rat islets isolated with collagenase were determined. …”
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Impact of sampling technique, anticoagulant, processing delay, and temperature on murine platelet function in whole blood by Silvia Maria Grazia Trivigno, Alice Assinger, Waltraud Cornelia Schrottmaier

“…Background: Platelets are highly sensitive to subtle changes in their microenvironment, making functional analyses challenging and prone to variation. …”
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Dynamic Expression and Functional Implications of the Cell Polarity Gene, Dchs1, During Cardiac Development by Kathryn Byerly, Cayla Wolfe, Hannah Parris, Charlotte Griggs, Emily Wilson, Matthew Huff, Molly Griggs, Jordan Morningstar, Lilong Guo, Fulei Tang, Jan Guz, Taylor Petrucci, Ranan Phookan, Brian Loizzi, Cortney Gensemer, Russell A. Norris

“…Intercellular interactions among cardiac cell populations are essential for cardiac morphogenesis, yet the molecular mechanisms orchestrating these events remain incompletely understood. Dachsous1 (Dchs1), an atypical cadherin linked to mitral valve prolapse, is a core planar cell polarity protein whose function in the developing heart has not been fully elucidated. …”
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Improvement of Methods for Evaluating Electrical Engineering Enterprises Functioning and Their Structural Components Based on Identical Indicators by Yakovlev A.I., Vasiltsova S.A., Larka L.S.

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A deep learning framework for predicting disease-gene associations with functional modules and graph augmentation by Xianghu Jia, Weiwen Luo, Jiaqi Li, Jieqi Xing, Hongjie Sun, Shunyao Wu, Xiaoquan Su

“…Advances in high-throughput biotechnology have generated a wealth of data linking diseases to specific genes. While graph representation learning has recently introduced groundbreaking approaches for predicting novel associations, existing studies always overlooked the cumulative impact of functional modules such as protein complexes and the incompletion of some important data such as protein interactions, which limits the detection performance. …”
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Maturation of Corpus Callosum Anterior Midbody Is Associated with Neonatal Motor Function in Eight Preterm-Born Infants by Preethi Mathew, Kerstin Pannek, Pamela Snow, M. Giulia D'Acunto, Andrea Guzzetta, Stephen E. Rose, Paul B. Colditz, Simon Finnigan

“…The etiology of motor impairments in preterm infants is multifactorial and incompletely understood. Whether corpus callosum development is related to impaired motor function is unclear. …”
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Structural and functional roles of conserved residues of human papillomavirus (HPV) E2 protein and biological consequences by Sean Fletcher, Esther E. Biswas-Fiss, Subhasis B. Biswas

“…The HPV E2 protein is a central regulator of viral replication and oncogene expression, making it a critical determinant of HPV-associated malignancies. While its core functions are conserved, variations within the E2 protein are thought to contribute to the differential oncogenic potential among HPV types, though the structural basis for this remains incompletely understood. …”
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