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    Fast growth and high-titer bioproduction from renewable formate via metal-dependent formate dehydrogenase in Escherichia coli by Aidan E. Cowan, Mason Hillers, Vittorio Rainaldi, Florent Collas, Hemant Choudhary, Basem S. Zakaria, Gregory G. Bieberach, David N. Carruthers, Maxwell Grabovac, Jennifer W. Gin, Bridgie Cawthon, Yan Chen, Emine Akyuz Turumtay, Edward E. K. Baidoo, Christopher J. Petzold, Adam M. Feist, Sara Tejedor-Sanz, Frank Kensy, Blake A. Simmons, Jay D. Keasling, Nico J. Claassens

    Published 2025-07-01
    “…So far, a major limitation for synthetic formatotrophy has been slow energy supply due to slow formate dehydrogenase activity. Here, we implement a fast, metal-dependent formate dehydrogenase complex in a synthetic formatotrophic Escherichia coli utilizing the reductive glycine pathway. …”
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