Anti-restriction functions of injected phage proteins revealed by peeling back layers of bacterial immunity by Sukrit Silas, Héloïse Carion, Kira S. Makarova, David Sanchez Godinez, Surabhi Haniyur, Lucy Volino, Wearn-Xin Yee, Andrea Fossati, Danielle Swaney, Michael Bocek, Eugene V. Koonin, Joseph Bondy-Denomy

“…Abstract Virus-host competition drives evolution of diverse antivirus defenses, but how they co-operate in wild bacteria and how bacteriophages circumvent host immunity remains poorly understood. Here, using a functional screening platform to systematically explore the functions of phage accessory genes, we describe how cell-surface barriers can obscure the phenotypes of intracellular defenses in E. coli isolates. …”
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Multi-omics-based subtyping of melanoma suggests distinct immune and targeted therapy strategies by Changchang Li, Xiaoqiong Lin, Jinhui Wang, Qiaochu Zhou, Fangfang Feng, Jie Xu

“…A precise molecular classification is critical for improving prognostic assessment and guiding personalized therapy.MethodsWe performed an integrative multi-omics analysis of skin cutaneous melanoma using data from The Cancer Genome Atlas (TCGA) and validated our findings in independent cohorts. Multi-layered data, including transcriptomic, genomic, epigenetic, and immune landscape profiles, were analyzed using unsupervised clustering and machine learning approaches to define molecular subtypes. …”
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Analysis of immune characteristics and inflammatory mechanisms in COPD patients: a multi-layered study combining bulk and single-cell transcriptome analysis and machine learning by Changjin Wei, Yongfeng Zhu, Caiming Chen, Feipeng Li, Li Zheng

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Chemical and Toxicological Diagnosis of Acute Poisonings with Phenazepam by M. V. Belova, E. A. Klyuyev, E. S. Melnikov, D. M. Yeliseyeva

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Eggshell Quality Traits and Transcriptome Gene Screening Between Yunnong and Jingfen Chicken Breeds by Zijian Li, Hao Wu, Jing Fu, Maida Mushtaq, Muhammad Khan, Yong Liu, Zobia Azeem, Hongmei Shi, Yang He, Ru Zhang, Muhammad Aziz Ur Rahman, Jiajia Kang, Changrong Ge, Kun Wang

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The combination of inactivated and subunit vaccines enhances protective efficacy against Mycoplasma synoviae by Chenyang Yi, Qiaoxia Xu, Yu Han, Mingyong Deng, Guohong Li, Chao Li, Xiaomei Sun, Ming Zhong, Meilin Jin, Chao Kang

“…Vaccination remains the most cost-effective strategy to protect layers from MS infection. Previous studies from our laboratory identified and screened immunogenic proteins of MS, culminating in the development of a subunit vaccine that provided robust protection with a two-dose immunization. …”
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Defects in intracellular trafficking of fungal cell wall synthases lead to aberrant host immune recognition. by Shannon K Esher, Kyla S Ost, Maria A Kohlbrenner, Kaila M Pianalto, Calla L Telzrow, Althea Campuzano, Connie B Nichols, Carol Munro, Floyd L Wormley, J Andrew Alspaugh

“…This cell wall remodeling is essential for host immune avoidance by this pathogen. In a genetic screen for mutants with changes in their cell wall, we identified a novel protein, Mar1, that controls cell wall organization and immune evasion. …”
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Pharmacognostic analysis and antimalarial evaluation of quercetin in Ilex umbellulata bark using HPTLC, in vitro screening, molecular docking, and network pharmacology by James H. Zothantluanga, Dipak Chetia, Yasangam Umbon, T. C. Lalhriatpuii, Dhritiman Roy, Nidahun Lamare, Salem Lalvenhimi

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Integrated analysis reveals key circRNA-mediated regulatory axes related to prognosis and immune infiltration in lung adenocarcinoma by Yan Pei, Kang Lin

“…The circRNA-mediated ceRNA subnetworks and a risk score were constructed through layer-by-layer screening and validating. Immune infiltration analysis was performed by CIBERSORT. …”
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A novel molecular classification system based on the molecular feature score identifies patients sensitive to immune therapy and target therapy by Yang Li, Yang Li, Yinan Ding, Yinan Ding, Jinghao Wang, Xiaoxia Wu, Xiaoxia Wu, Dinghu Zhang, Dinghu Zhang, Han Zhou, Han Zhou, Pengfei Zhang, Pengfei Zhang, Guoliang Shao, Guoliang Shao

“…Feature scores (FSs) from hallmark pathways, regulatory cell death pathways, metabolism pathways, stemness index, immune scores, estimate scores, etc. were evaluated and screened. …”
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Antitrypanosomal and Antileishmanial Activities of <i>Tacca leontopetaloides</i> Tubers and <i>Zanthoxylum zanthoxyloides</i> Stem Bark by Elizabeth O. Agbo, John V. Anyam, Cyprian T. Agber, Christie A. Adah, Christopher Agbo, Augustina O. Ijeomah, Terrumun A. Tor-Anyiin, Hamed E. Alkhalaf, Aditya Sarode, Jamal I. Asseri, Alexander I. Gray, John O. Igoli, Harry P. De Koning

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Retinal degeneration mutation in Sftpa1tm1Kor/J and Sftpd -/- targeted mice. by Faizah Bhatti, Johannes W Kung, Frederico Vieira

“…Retinas from Sftpa1tm1Kor/J and Sftpd -/- mice showed early retinal degeneration with loss of the outer nuclear layer. After screening of mice for known retinal degeneration mutations, the mice were found to carry a previously unrecognized Pde6brd1 genotype which resulted from earlier breeding of the strain with Black Swiss mice during their generation. …”
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Biofilm morphology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) on poly-D,L-lactide-co-poly(ethylene glycol) (PDLLA-PEG) coated titanium by Adam Benedict Turner, David Zermeño-Pérez, Margaritha M. Mysior, Paula Milena Giraldo-Osorno, Begoña García, Elizabeth O'Gorman, Shafik Oubihi, Jeremy C. Simpson, Iñigo Lasa, Tadhg Ó Cróinín, Margarita Trobos

“…In this study, titanium surfaces (Ti) were coated with poly-D,L-lactide (PDLLA, (P)), polyethylene-glycol poly-D,L-lactide (PEGylated-PDLLA, (PP20)), or multi-layered PEGylated-PDLLA (M), with or without 1 % silver sulfadiazine. …”
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Tri-culture model of intestinal epithelial cell, macrophage, and bacteria for the triggering of inflammatory bowel disease on a microfluidic device by Shiori Tamura, Clarissa Ellice Talitha Pasang, Minami Tsuda, Shilan Ma, Hiromasa Shindo, Noriyuki Nagaoka, Tomoki Ohkubo, Yoichi Fujiyama, Miho Tamai, Yoh-ichi Tagawa

“…Inflammatory bowel disease (IBD) involves gastrointestinal inflammation, due to intestinal epithelial barrier destruction caused by excessive immune activation. Conventional cell culture systems do not provide a model system that can recapitulate the complex interactions between epithelial cells, immune cells, and intestinal bacteria. …”
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Gastrointestinal Motility and Improvement Efficacy of Shenhuang Plaster Application on Shenque: Identification, Evaluation, and Mechanism by Yanan Shi, Jingming Xu, Bin Ding, Guiping Chen, Lu Jin, Liang Ke, Xiao Xu, JingXia Wang, Qiuhua Sun, Xiaohong Xu

“…PTX-induced sluggish intestine, villus, and mucosal base layer damage were significantly improved following the application of SHP. …”
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Dynamics understanding of novel solvated drug molecules against emerging Burkholderia Cepacia infections in immunocompromised patients by Sajjad Ahmad, Faisal Ahmad, Syed Ainul Abideen, Kalsoom Khan, Muhammad Irfan, Farhan Siddique, Norah Abdullah Albekairi, Abdulrahman Mohammed Alshammari, Dong-Qing Wei

“…The bacterial tyrosine kinase enzyme takes part in exopolysaccharides production to form a biofilm matrix layer and thus results in the promotion of aggressive and resistant infections. …”
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Artificial intelligence in breast oncology by Alexander Mundinger

“…Abstract Artificial intelligence (AI) is based on complex artificial neural networks, characterized by layered network architecture, parallel processing of large data sets and iterative algorithms for processing large data sets. …”
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Pan-Genomic Insights into Rumen Microbiome-Mediated Short-Chain Fatty Acid Production and Regulation in Ruminants by Jingyi Shi, Hongren Su, Shichun He, Sifan Dai, Huaming Mao, Dongwang Wu

“…By integrating multi-omics datasets, pan-genomic approaches unveil unprecedented layers of microbial diversity, enabling precise identification of core functional genes and their dynamic contributions to carbohydrate degradation and SCFA biosynthesis. …”
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Integrated multiomics analysis and machine learning refine molecular subtypes and prognosis for thyroid cancer by Peng Zhang, Meizhong Qin, Fen Li, Kunpeng Hu, He Huang, Cuicui Li

“…Methods We integrated five omics layers from THCA patients using eleven clustering algorithms to identify molecular subtypes. …”
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MOG-specific CAR Tregs: a novel approach to treat multiple sclerosis by Jihane Frikeche, Marion David, Xavier Mouska, Damien Treguer, Yue Cui, Sandrine Rouquier, Enora Lecorgne, Emma Proics, Papa Babacar Fall, Audrey Lafon, Gregory Lara, Alexandra Menardi, David Fenard, Tobias Abel, Julie Gertner-Dardenne, Maurus de la Rosa, Celine Dumont

“…Abstract Multiple sclerosis (MS) is an autoimmune disease affecting the central nervous system (CNS) with the immune system attacking myelin sheaths leading to neuronal death. …”
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