Genotypic clustering of 51 soybean cultivars and wild forms using SSR-markers by Alena A. Ivaniy, Andrey A. Penzin, Olga N. Bondarenko, Anastasia A. Blinova, Alina E. Gretchenko, Lyubov E. Ivachenko, Pavel D. Timkin

“…Genomic DNA was isolated from the studied samples and then amplified. …”
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Comparative performance evaluation of bisulfite- and enzyme-based DNA conversion methods by Roy B. Simons, Faidra Karkala, Marta M. Kukk, Hieab H. H. Adams, Manfred Kayser, Athina Vidaki

“…Finally, we converted 10 ng of 22 genomic DNA samples using both methods. We observed an overestimation of the BC DNA recovery (130%) and a low recovery for EC (40%). …”
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Bacterial interactions underpin worsening lung function in cystic fibrosis-associated infections by Damian W. Rivett, Lauren R. Hatfield, Helen Gavillet, Michelle Hardman, Christopher van der Gast

“…To confirm this, pooled samples from studies conducted by our laboratory, sequenced using the same pipeline, were used to assess microbiome-wide associations to lung function. …”
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Rare serovars of non-typhoidal Salmonella enterica isolated from humans, beef cattle and abattoir environments in Nigeria. by Mabel Kamweli Aworh, Pernille Nilsson, Beverly Egyir, Felicia Amoa Owusu, Rene S Hendriksen

“…Among 22 NTS, 131 mobile genetic elements (MGEs) were detected including insertion sequences (56.5%) and miniature inverted repeats (43.5%). …”
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New molecular diagnostic targets for Avibacterium paragallinarum and a set of single-plex and multiplex qPCR methods for the rapid differential diagnosis of Mycoplasma gallisepticu... by Bin Xu, Shu Wang, Weiping Yao, Bo Ni, Ting Yuan, Beibei Liu, Long Yuan, Yanna Wei, Sunting Ma, Lixin Lyu, Xiaoli Wang, Wei Ouyang, Zhenzhen Zhang, Zhixin Feng

“…The intra-group and inter-group variation coefficients of the qPCR methods established in this study were all within 2 % in the repeatability evaluation. In terms of the coincidence rate of clinical sample testing, the qPCR methods showed 100 % detection consistency for the clinical samples tested. …”
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Distinguishing between wild-caught and captive-bred Common Pheasant using methylation rate of skeletal muscle DNA by Wenhui Wang, Lijun Lin, Yue Ma, Yan Cui, Qi Zhang, Jincheng Yang, Yongheng Zhou, Liangyu Cui, Boyang Liu, Chang Su, Mengjia Yu, Yuwei Gao, Peng Gao, Yujia Du, Yu Zhou, Elizabeth Kamili, Shuhui Yang, Yanchun Xu

“…A total of 13,507 differentially methylated regions were identified between five wild-caught and five captive-bred individuals through whole-genome methylation sequencing (WGBS). Based on the selected five methylation sites, LOC116231076, LOC116242223, ATAD2B, EGFL6, and HS2ST, quantitative detection technique was developed using methylation-sensitive high-resolution melting curve (MS-HRM) to measure methylation rates. …”
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Surveillance for Infection and Colonization due to Candida spp. at a Tertiary Referral Hospital in Botswana: 2022-2024 by Ms Teresia Gatonye, Ms Janet John-Thubuka, Mr Tichaona Machiya, Ms Tlhalefo Dudu Ntereke, Mr Tshiamo Zankere, Mrs Chimwemwe V Tembo, Ms Boingotlo Gopolang, Dr Naledi Mannathoko, Ms Miriam Mwamba, Prof Nelesh Govender, Ms Sabelle Jallow, Ms Rudzani Mashau, Ms Ruth Mpembe, Ms Silondiwe Nzimande, Dr Melissa Richard-Greenblatt, Dr David Goldfarb, Dr Ebbing Lautenbach, Dr Jonathan Strysko

“…Further analysis with whole genome sequencing could be used to produce a phylogenetic comparison with other clinical isolates in the southern African region.…”
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Disturbances of the Gut Microbiota, Sleep Architecture, and mTOR Signaling Pathway in Patients with Severe Obstructive Sleep Apnea-Associated Hypertension by Chih-Yuan Ko, Huan-Zhang Su, Li Zhang, Yi-Ming Zeng

“…Results revealed that severe-OSA patients with hypertension had an altered gut microbiome, decreased short-chain fatty acid-producing bacteria P<0.05, and reduced arginine and proline metabolism pathways P=0.001, compared with controls; also, they had increased stage N1 sleep and reduced stages N2 and N3 sleep accompanied by repeated arousals P<0.05. Analysis of PBMCs using the Kyoto Encyclopedia of Genes and Genomes database showed that the mTOR signaling pathway P=0.006 was the most important differential gene-enriched pathway in severe-OSA patients with hypertension. …”
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Effective Newborn Screening for Type 1 and 3 Primary Hyperoxaluria by Bernd Hoppe, Cristina Martin-Higueras, Lodovica Borghese, Sophie Kaspar, Björn Reusch, Bodo B. Beck, Adam Walli, Ella Janzen, Sebastian Hegert, Nils Janzen, Katharina Hohenfellner

“…Heterozygous AGXT/HOGA1 carriers received repeated spot urine analyses and, in case of persistent hyperoxaluria, complete Sanger sequencing of AGXT and HOGA1 genes, respectively. …”
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Treatment of colorectal peritoneal metastases with oxaliplatin induces biomarkers predicting response to immune checkpoint blockade by Alexander Constantinides, Nico Lansu, Peter Mosen, Paulien Rauwerdink, Esther Strating, Franziska Völlmy, Maaike Nederend, Jeanette H.W. Leusen, Koen Rovers, Emma Wassenaar, Robin Lurvink, Maarten Altelaar, Simon Nienhuijs, Rene Wiezer, Inne H.M. Borel Rinkes, Djamila Boerma, Geert J.P.L. Kops, Ignace de Hingh, Onno Kranenburg

“…The biobank contains biopsies from 3 PM per patient, repeatedly sampled prior to each treatment cycle, and ascites. …”
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Multiple myeloma with extramedullary plasmacytoma: pathogenesis and clinical case by M. V. Firsova, N. V. Risinskaya, M. V. Solovev, T. N. Obukhova, M. A. Kislitsyna, E. E. Nikulina, I. A. Yakutik, T. V. Abramova, A. B. Sudarikov, A. M. Kovrigina, L. P. Mendeleeva

“…The mutation profile of the KRAS, NRAS, BRAF genes was studied by Sanger sequencing in tumor samples of various localizations.Results. …”
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