Hypermethylation of CDKN2A CpG island drives resistance to PRC2 inhibitors in SWI/SNF loss-of-function tumors by Xinghao Wang, Yajun Wang, Min Xie, Shichao Ma, Yilin Zhang, Lele Wang, Yangfeng Ge, Guobin Li, Mengxi Zhao, Sheng Chen, Chenxi Yan, Hailong Zhang, Wei Sun

“…Previous studies have suggested that PRC2 is important for the proliferation of tumors with SWI/SNF loss-of-function mutations. In the present study, we have developed an EED-directed allosteric inhibitor of PRC2 termed BR0063, which exhibits anti-proliferative properties in a subset of solid tumor cell lines harboring mutations of the SWI/SNF subunits, SMARCA4 or ARID1A. …”
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TiO2-ZnPc nanoparticles functionalized with folic acid as a target photosensitizer for photodynamic therapy against glioblastoma cells by Gustavo Jardón-Guadarrama, Ma Elena Manríquez-Ramírez, Citlali E. Rodríguez-Pérez, Araceli Díaz-Ruiz, María de los Ángeles Martínez-Cárdenas, Alfonso Mata-Bermudez, Camilo Ríos, Emma Ortiz-Islas

“…The TiO2 surface has been modified with different functional groups to achieve activation at longer wavelengths (visible light). …”
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Identification of prognostic hub genes and functional role of BAIAP2L2 in prostate cancer progression: a transcriptomic and experimental study by Xiangyang Zhan, Xiangyang Zhan, Xiangyang Zhan, Xiangyang Zhan, Wenkai Wang, Jie Lian, Yichun Li, Jianyi Gu, Jianyi Gu, Jianyi Gu, Jianyi Gu, Dongdong Guo, Dongdong Guo, Dongdong Guo, Dongdong Guo, Dongliang Xu, Dongliang Xu, Dongliang Xu, Dongliang Xu, Guanqun Ju, Guanqun Ju, Guanqun Ju, Guanqun Ju

“…In vitro knockdown experiments were conducted in PC3 and DU145 cell lines to assess BAIAP2L2’s functional role through assays for migration, colony formation, and proliferation.ResultsA total of 1,449 DEGs were identified, including 775 upregulated and 674 downregulated genes. …”
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Functional Role of Fatty Acid Synthase for Signal Transduction in Core-Binding Factor Acute Myeloid Leukemia with an Activating c-Kit Mutation by Ruimeng Zhuang, Bente Siebels, Konstantin Hoffer, Anna Worthmann, Stefan Horn, Nikolas Christian Cornelius von Bubnoff, Cyrus Khandanpour, Niklas Gebauer, Sivahari Prasad Gorantla, Hanna Voss, Hartmut Schlüter, Malte Kriegs, Walter Fiedler, Carsten Bokemeyer, Manfred Jücker, Maxim Kebenko

“…Functional implications, including cell proliferation, were assessed through Western blotting, mass spectrometry, and PamGene. …”
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RUBY® – a tetravalent (2+2) bispecific antibody format with excellent functionality and IgG-like stability, pharmacology and developability properties by Barnabas Nyesiga, Mattias Levin, Anna Säll, Anna Rosén, Kim Jansson, Sara Fritzell, Karin Hägerbrand, Dietmar Weilguny, Laura von Schantz

“…In all, the RUBY format has attractive mAb-like attributes and offers the possibility to mitigate many of the development challenges linked to other bsAb formats, facilitating both high functionality and developability.…”
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Curcumin derivative C210 induces Epstein–Barr virus lytic cycle and inhibits virion production by disrupting Hsp90 function by Linli Chen, Xiaojing Guo, Wen Lin, Yingying Huang, Suling Zhuang, Qianfeng Li, Jianhua Xu, Shengnan Ye

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Identification and functional validation of a new gene conferring resistance to Soybean Mosaic Virus strains SC4 and SC20 in soybean by Muhammad Muzzafar Raza, Huiying Jia, Muhammad Khuram Razzaq, Bowen Li, Kai Li, Junyi Gai

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SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation by Ryan T. Wagner, Ryan A. Hlady, Xiaoyu Pan, Liguo Wang, Sungho Kim, Xia Zhao, Louis Y. El Khoury, Shafiq Shaikh, Jian Zhong, Jeong-Heon Lee, Jolanta Grembecka, Tomasz Cierpicki, Thai H. Ho, Keith D. Robertson

“…Conclusions These findings unify genome-wide screening approaches with the latest genetic and pharmacologic modeling methodologies to reveal an entirely novel epigenetic approach to individualize therapies against a challenging loss-of-function SETD2 mutation in cancer.…”
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Applying Unbiased, Functional Criteria Allows Selection of Novel Cyclic Peptides for Effective Targeted Drug Delivery to Malignant Prostate Cancer Cells by Anna Cohen, Maysoon Kashkoosh, Vipin Sharma, Akash Panja, Sagi A. Shpitzer, Shay Golan, Andrii Bazylevich, Gary Gellerman, Galia Luboshits, Michael A. Firer

“…Peptides from the internalized clones were then subjected to a panel of biochemical and functional tests that led to the selection of several peptide candidates. …”
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The combined effect of Pdx1 overexpression and Shh manipulation on the function of insulin‐producing cells derived from adipose‐tissue stem cells by Mahmoud Hashemi Tabar, Mohammad Reza Tabandeh, Eskandar Moghimipour, Dian Dayer, Ata A. Ghadiri, Elham Allah Bakhshi, Mahmoud Orazizadeh, Mohammad Ali Ghafari

“…The functionality of IPCs in vitro was analyzed through dithizone (DTZ) staining and ELISA. …”
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Using Plant DNA Barcodes and Functional Traits to Assess Community Assembly of Quercus Forests at Different Scales in the Semiarid Loess Plateau of China by YongFu Chai, TingTing Tian, Luyao Wang, Junxin Wei, Yao Xu, Peiliang Liu, Chengcheng Xiang, Ming Yue

“…Utilizing two plastid DNA genes (rbcL and matK) and one nuclear DNA gene (internal transcribed spacer, ITS), we first constructed the phylogenies of 147 woody species from 98 line transects in the forest areas of the Loess Plateau and subsequently measured three functional traits. …”
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RETRACTED ARTICLE: LRPPRC regulates malignant behaviors, protects mitochondrial homeostasis, mitochondrial function in osteosarcoma and derived cancer stem-like cells by Ziyi Zhao, Yingwei Sun, Jing Tang, Yuting Yang, Xiaochao Xu

“…Conclusion Taken together, these findings indicated that LRPPRC exerts critical roles in regulating mitochondrial homeostasis, mitochondrial function and tumorigenesis in osteosarcomas and osteosarcoma-derived CSCs. …”
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Genome-wide identification of ARID-HMG related genes in citrus and functional analysis of FhARID1 in apomixis and axillary bud development by Xietian Song, Yin Zhou, Zhen Cao, Nan Wang, Xiaoyu Tian, Lijun Chai, Zongzhou Xie, Junli Ye, Xiuxin Deng

“…Yeast two-hybrid and MS/MS spectra further indicated that FhARID1 function requires casein kinase II-mediated post-transcriptional phosphorylation. …”
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tRF-5028c disrupts trophoblast function in recurrent spontaneous abortion by inhibiting CRKL-mediated Rap1 signaling pathway by Jialyu Huang, Jiawei Wang, Shuang Wang, Xiangpeng Xiong, Ruiyin Jiang, Chaoyi Xiong, Lu Wang, Lingling Huang, Yan Zhao, Zheng Fang, Xiaoyan Ai, Jiaying Lin

“…Conclusions tRF-5028c upregulation impaired trophoblast function to facilitate RSA development by directly targeting CRKL-mediated Rap1 pathway. …”
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Cloning and Functional Analysis of <i>BraTSD2</i> Associated with Root Swelling in Turnip (<i>Brassica rapa</i> L.) by Wenyue Huang, Shifan Zhang, Fei Li, Hui Zhang, Rifei Sun, Guoliang Li, Shujiang Zhang

“…In this study, we cloned the turnip root swelling-related gene <i>BraTSD2</i> (<i>TUMOROUS SHOOT DEVELOPMENT 2</i>) and performed a preliminary functional analysis. The bioinformatics analysis of <i>BraTSD2</i> indicated that the protein is likely to possess a transmembrane feature. …”
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Identifying trade-offs and synergies among land use functions using an XGBoost-SHAP model: A case study of Kunming, China by Kun Li, Junsan Zhao, Yongping Li, Yilin Lin

“…The interaction between ecological function (EF) and production function (PF), as well as between living function (LF) and PF, showed a convex function relationship with evident boundary effects. …”
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Comparative efficacy of mind–body exercise for pain, function, quality of life in knee osteoarthritis: a systematic review and network meta-analysis by Kaixia Gao, Jinmeng Tao, Guangyuan Liang, Chen Gong, Lin Wang, Yuling Wang

“…Randomized clinical trials comparing MBE interventions for pain, physical function and quality of life (QoL) in KOA patients were eligible. …”
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Comprehensive prognostic gene identification and functional characterization of GRAMD1A in Wilms tumor: development of risk prediction models and therapeutic implications by Qiang Zeng, Junfeng Tao, Lilu Qin, Yong Zeng, Zhong Liu, Mingxian Xu, Linshan Zeng

“…External validation confirmed key hub genes, while functional assays in WT cell lines (WiT-49) assessed the role of GRAMD1A in tumor behavior.ResultsA total of 3,395 DEGs were identified, with 1,564 upregulated and 1,831 downregulated genes. …”
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Targeting CD16A on NK cells and GPC3 in hepatocellular carcinoma: development and functional validation of a therapeutic bispecific antibody by Liu Chen, Yuankui Zhu, Mingqian Feng, Mingqian Feng, Dianbao Zuo, Guoping Chen, Kangkang Ji, Kangkang Ji

“…It demonstrated potent, dose-dependent cytotoxicity against GPC3+ HCC lines (HepG2/Huh7/Hep3B, IC50 = 15–35 ng/mL) via NK cell activation, surpassing conventional antibody-dependent cellular cytotoxicity (ADCC). …”
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Synergistic Effects of Low-Frequency Ultrasound and Therapeutic Agents on Endothelial and Renal Cells: Emphasis on Cell Functionality, Oxidative Stress, and Inflammatory Markers by Ieva Čiapienė, Joris Vėžys, Vaiva Lesauskaitė, Indrė Matulevičiūtė, Ugnė Meškauskaitė, Vilius Skipskis, Arvydas Strazdauskas, Sonata Trumbeckaitė, Algimantas Bubulis, Vytautas Jūrėnas, Vytautas Ostaševičius, Vytenis Tamakauskas, Vacis Tatarūnas

“…Cell viability and wound-healing assays assessed cellular function, while nitric oxide (NO) and reactive oxygen species (ROS) assays were used to evaluate redox signaling. …”
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