Elamipretide enhances post-thaw rooster sperm quality by mitigating oxidative stress and optimizing mitochondrial function during cryopreservation by Abouzar Najafi, Seyed Davood Sharifi, Ramin Farhadi, Shorsh Hussein Bapir

“…Mitochondrial activity peaked at 9 µmol/L (60.21 ± 1.92), reflecting enhanced cell vitality and function. However, the effects were diminished at 12 µmol/L, indicating toxicity at higher concentrations. …”
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Early life ozone exposure results in dysregulated innate immune function and altered microRNA expression in airway epithelium. by Candice C Clay, Kinjal Maniar-Hew, Joan E Gerriets, Theodore T Wang, Edward M Postlethwait, Michael J Evans, Justin H Fontaine, Lisa A Miller

“…Assessment of potential IL-6-targeting microRNAs miR-149, miR-202, and miR-410 showed differential expression in primary cultures based upon animal exposure history. Functional assays revealed that miR-149 is capable of binding to the IL-6 3' UTR and decreasing IL-6 protein synthesis in airway epithelial cell lines. …”
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FAM20B Gain-of-Function Blocks the Synthesis of Glycosaminoglycan Chains of Proteoglycans and Inhibits Proliferation and Migration of Glioblastoma Cells by Lydia Barré, Irfan Shaukat, Mohamed Ouzzine

“…Interestingly, we bring evidence that, in contrast to FAM20B, expression of the 2-phosphoxylose phosphatase XYLP increases, in a dose dependent manner, GAG chain synthesis and rescues the blockage of GAG chains synthesis induced by FAM20B. In line with previous reports, we found that <i>FAM20B</i> loss-of-function reduced GAG chain synthesis. …”
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Rural Water Point Functionality Estimates and Associations: Evidence From Nine Countries in Sub‐Saharan Africa and South Asia by Anna L. Murray, Gabriela Stone, Audrey R. Yang, Nicholas F. Lawrence, Hannah Matthews, Georgia L. Kayser

“…Water points in communities with fewer available water points, those with operational water management committees (which meet often, collect fees, and can name someone for maintenance), and those with financial savings were associated with higher functionality. As piped water system provision increases in line with international development goals, our results suggest that the benefits of providing higher levels of water service should be carefully considered alongside the technological, institutional, and financial investment required to operate and maintain more complex piped water systems in rural settings.…”
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Multi-omics analysis reveals cancer stemness function and establishes a predictive model for thyroid cancer prognosis and immunotherapy by Zhanrong Feng, Yue Xu, Ying Ding, Xiaoxiao Sun, Qian Zhao, Jingjing Zhu, Qiang Chen, Yang Zhang, Yilai Zhang

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Selective molecular inhibition of the HDAC6 ZnF-UBP binding domain impairs multiple myeloma cell function by Rafael Rincón, Isabel F. Coira, Antoine Richieu, Fedaa Attana, Muriel Urwyler, Shozeb Haider, Carole Bourquin, Philippe Bertrand, Muriel Cuendet

“…A multiple myeloma cell line containing the non-functional ZnF-UBP domain was then engineered. …”
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Ago HITS-CLIP expands understanding of Kaposi's sarcoma-associated herpesvirus miRNA function in primary effusion lymphomas. by Irina Haecker, Lauren A Gay, Yajie Yang, Jianhong Hu, Alison M Morse, Lauren M McIntyre, Rolf Renne

“…A detailed understanding of KSHV miRNA functions requires high-through putribonomics to globally analyze putative miRNA targets in a cell type-specific manner. …”
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Gene Localization and Functional Validation of <i>GmPDH1</i> in Soybean Against Cyst Nematode Race 4 by Yuehua Dai, Yue Zhang, Chuhui Li, Kun Wan, Yan Chen, Mengen Nie, Haiping Zhang

“…To identify the key genes conferring resistance to soybean cyst nematode race 4 (SCN4, <i>Heterodera glycines</i>), this study utilized 280 recombinant inbred lines (RILs) derived from the resistant cultivar Huipizhiheidou (HPD) and the susceptible cultivar Jindou23 (JD23). …”
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Trans-ancestry transcriptome-wide association and functional studies to uncover novel susceptibility genes and therapeutic targets for colorectal cancer by Lijuan Wang, Lidan Hu, Jing Sun, Jianhui Zhao, Siyun Zhou, Lexin Liu, Wei Yu, Yeting Hu, Dan Zhou, Xiangrui Meng, Zhongshang Yuan, Honghe Zhang, Susan Farrington, Maria Timofeeva, Kefeng Ding, Julian Little, Malcolm Dunlop, Evropi Theodoratou, Xue Li

“…We first conducted a trans-ancestry transcriptome-wide association study (TWAS) among 57,402 CRC cases and 119,110 controls, aiming to examine how altered gene expression influences CRC risk in European and Asian populations. Then, functional experiments in (i) CRC cell lines and (ii) tumor xenografts were conducted to examine potential underlying mechanisms involved in colorectal carcinogenesis. …”
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The Aryl Hydrocarbon Receptor (AhR) Is a Novel Gene Involved in Proper Physiological Functions of Pancreatic β-Cells by Shuhd Bin Eshaq, Jalal Taneera, Shabana Anjum, Abdul Khader Mohammed, Mohammad H. Semreen, Karem H. Alzoubi, Mohamed Eladl, Yasser Bustanji, Eman Abu-Gharbieh, Waseem El-Huneidi

“…This study aimed to investigate the expression of AhR in human pancreatic islets using publicly available RNA-sequencing (RNA-seq) databases and to explore its correlations with various metabolic parameters and key β-cell markers. Additionally, functional experiments were conducted in INS-1 cells, a rat β-cell line, to elucidate the role of Ahr in β-cell biology. …”
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Functional characterisation of CD8+ T cells mobilised with acute supramaximal high‐intensity interval exercise: implications for immune surveillance by Anna Strömberg, Mirko Mandić, Brennan J Wadsworth, Sebastian Proschinger, Seher Alam, Lisa MJ Eriksson, Laura Barbieri, Eric Rullman, Helene Rundqvist

“…Importantly, CD8+ T cells maintained their viability and expansion properties despite the metabolic challenges induced by exercise. Functionally, post‐exercise CD8+ T cells showed increased release of IFN‐gamma and an enhanced unspecific cell killing capacity as demonstrated by co‐culture with the immortalised cell line HEK293T. …”
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Functionalization of surfactant templated magnetite by chitosan and PEGylated/Chitosan – In vitro studies on drug loading, release and anti-proliferative activity by Nasima Arshad, Maliha Khan, Muhammad Waseem, Mohd Afzal, Abdullah Alarifi, Rahman Shah Zaib Saleem

“…The drug release was found greater at pH 5.0 for all functionalized MNPs. However, among all functionalized MNPs, maximum drug release was found to be 78.88% by MPCT-1 in acidic medium (pH 5.0). …”
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Expression of recombination antimicrobial protein PIL22-PBD-2 in Pichia pastoris and verification of its biological function in vitro by Xian Li, Pengfei Qiu, Menglong Yue, Ying Zhang, Congshang Lei, Jingyu Wang, Xiwen Chen, Xuefeng Qi

“…Additionally, given its strong ability to repair intestinal epithelial damage and maintain intestinal mucosal barrier function, porcine interleukin-22 (PIL-22) is a potential feed additive to combat intestinal damage caused by intestinal pathogens in piglets. …”
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Kyphoplasty as a Treatment Option for Traumatic Burst Fractures: A Case Series Evaluating Patient Outcomes and Functional Benefits by Anoop S. Chinthala, Barnabas Obeng-Gyasi, Trenton A. Line, Matthew K. Tobin, Gordon Mao, Bradley N. Bohnstedt

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Effective unilateral/bilateral robot-assisted training for upper limb motor function rehabilitation: a cross-sectional study by Guang Feng, Guang Feng, Guohong Chai, Guohong Chai, Jiaji Zhang, Jiaji Zhang, Tao Song, Tao Song, Changcheng Shi, Changcheng Shi, Jialin Xu, Jialin Xu, Guokun Zuo, Guokun Zuo

“…The objective of this study was to investigate the optimal robot-assisted training strategies for the upper limb functional recovery in hemiparetic stroke patients.ApproachA bilateral upper limb rehabilitation robot was employed to execute unilateral and bilateral training. …”
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The Phytochemical and Functional Characterization of the Aerial Parts of <i>Artemisa alba Turra</i> (<i>Asteraceae</i>) Grown in Romania by Mădălina Țicolea, Raluca Maria Pop, Marcel Pârvu, Lia-Oxana Usatiuc, Ana Uifălean, Valeria Alvarez Brito, Eva Fischer-Fodor, Floricuța Ranga, Crina Claudia Rusu, Maria Crisan, Bianca Bosca, Florinela Adriana Cătoi, Alina Elena Pârvu

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Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway by Mei Yang, Wangbin Xu, Chaofu Yue, Rong Li, Xian Huang, Yongjun Yan, Qinyong Yan, Shisheng Liu, Yuan Liu, Qiaolin Li

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Deficient AMPK-SENP1-Sirt3 signaling impairs mitochondrial complex I function in Parkinson’s disease model by Xiaoyu Sun, Jianyi Shen, Yimei Shu, Tianshi Wang, Lu He, Ruinan Shen, Yifan Zhou, Jinke Cheng, Suzhen Lin, Jianqing Ding

“…However, the pathway(s) through which MPTP impairs mitochondrial CI function remain to be elucidated. In this study, we aim to identify the molecular mechanisms through which MPTP modulates CI function and define the specific subunits of mitochondrial CI affected by MPTP. …”
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The integrative genomic and functional immunological analyses of colorectal cancer initiating cells to modulate stemness properties and the susceptibility to immune responses by Issam Tout, Salim Bougarn, Mohammed Toufiq, Neha Gopinath, Ola Hussein, Abbirami Sathappan, Evonne Chin-Smith, Fazulur Rehaman, Rebecca Mathew, Lisa Mathew, Kun Wang, Li Liu, Abdulrahman Salhab, Oleksandr Soloviov, Sara Tomei, Waseem Hasan, Sahar Da’as, Yosra Bejaoui, Nady El Hajj, Karama Makni Maalej, Said Dermime, Kakil Rasul, Paolo Dellabona, Giulia Casorati, Alice Turdo, Matilde Todaro, Giorgio Stassi, Soldano Ferrone, Xinhui Wang, Cristina Maccalli

“…Methods We conducted a multi-omics analysis—encompassing DNA methylation, total RNA sequencing, and microRNAs (miRNAs; N = 800) profiling on primary CICs and differentiated tumor cell lines, including autologous pairs. Functional immunological assays were performed to assess the impact of miRNA modulation. …”
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Gold complex QB1561 suppresses drug-resistant cancer cells by inhibiting TrxR and mitochondrial respiratory function by Hai-Ling Gao, Wenwen Ding, Zhi-Xin Shen, Qingbin Cui, Qingbin Cui

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