Development of a Caco-2-based intestinal mucosal model to study intestinal barrier properties and bacteria–mucus interactions by Evelien Floor, Jinyi Su, Maitrayee Chatterjee, Elise S. Kuipers, Noortje IJssennagger, Faranak Heidari, Laura Giordano, Richard W. Wubbolts, Silvia M. Mihăilă, Daphne A. C. Stapels, Yvonne Vercoulen, Karin Strijbis

“…This novel in vitro intestinal model can facilitate studies into mucus and epithelial barrier functions and in-depth molecular characterization of pathogenic and commensal microbe–mucus interactions.…”
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Transcription factor ZNF266 suppresses cancer progression by modulating CA9-mediated intracellular pH alteration in lung adenocarcinoma by Shencheng Ren, Junkan Zhu, Guangyao Shan, Jiaqi Liang, Yunyi Bian, Han Lin, Haochun Shi, Binyang Pan, Guangyin Zhao, Huiqin Yang, Xiaolong Huang, Cheng Zhan, Di Ge, Guoshu Bi

“…To validate this finding, we conducted a retrospective analysis of nearly three thousand LUAD patients’ data from public databases and our institution. Functional studies were performed using cell lines, organoids, and xenograft models to assess the role of ZNF266 in LUAD progression. …”
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Green Synthesis of Zinc Oxide Nanoparticles with <i>Psidium cattleianum</i> Leaves Extracts as Reducing Agent: Influence of Extraction Method on Physicochemical and Biological Acti... by Christian Israel Padilla-Hernández, Jorge Manuel Silva-Jara, Martha Reyes-Becerril, Abril Fonseca-García, Luis Miguel Anaya-Esparza, Paulo Roberto Orozco-Sánchez, Juan José Rivera-Valdés, Mireille López-Orozco, Carlos Arnulfo Velázquez-Carriles, María Esther Macías-Rodríguez

“…This could be correlated with different functional groups via FTIR and with thermal events associated with thermogravimetric curves, where the total biomass weight loss was lower for nanoparticles using ultrasound extract (6.25%), followed by maceration (15.55%) and infusion (18.01%) extracts. …”
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Inflammation-induced lysosomal dysfunction in human iPSC-derived microglia is exacerbated by APOE 4/4 genotype by Marianna Hellén, Isabelle Weert, Stephan A. Müller, Noora Räsänen, Pinja Kettunen, Šárka Lehtonen, Michael Peitz, Klaus Fließbach, Mari Takalo, Marja Koskuvi, Stefan F. Lichtenthaler, Ville Leinonen, Alfredo Ramirez, Olli Kärkkäinen, Mikko Hiltunen, Jari Koistinaho, Taisia Rõlova

“…Methods Altogether, microglia-like cells (iMGs) from eight APOE3/3 and six APOE4/4 human induced pluripotent stem cell (iPSC) lines were used in this study. The responses of iMGs to Aβ42, LPS and IFNγ were studied by metabolomics, proteomics, and functional assays. …”
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Isolation and Characterization of L-Asparaginase-Producing Bacteria from the Arabian–Persian Gulf Region: First Report on <i>Bacillus xiamenensis</i> ASP-J1-4 as a Producer and Its... by Ghofran M. Al-Harbi, Essam Kotb, Abeer A. Almiman, Mahmoud M. Berekaa, Salwa Alhamad, Nada F. Alahmady, Meneerah A. Aljafary, Nadiyah M. Alqazlan, Reem I. Alyami, Joud M. Alqarni, Ebtesam Abdullah Al-Suhaimi

“…L-asparaginase (L-ASNase) functions as a chemotherapeutic enzyme with antitumor properties. …”
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USP7 promotes temozolomide resistance by stabilizing MGMT in glioblastoma by Jiabing Li, Xiaorong Feng, Zhaohui Liu, Yunfang Deng, Zhiming Sun, Bei Chen, Lihui Wu, Xiaolong Wang, Lin Miao, Liyuan Zeng, Lei Hu, Yuming He, Ying Sheng, Yue Liu, Yu Zhao

“…USP7 binds directly to MGMT via its UBL domain, counteracts K48-linked ubiquitin chains, and prevents MGMT proteasomal degradation. This functional relationship is further supported by their nuclear colocalization. …”
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Proteomic profiling improves prognostic risk stratification of the Sarculator nomogram in soft tissue sarcomas of the extremities and trunk wall by Madhumeeta Chadha, Sara Iadecola, Andrew Jenks, Valeriya Pankova, Yuen Bun Tam, Jessica Burns, Amani Arthur, Christopher P. Wilding, Liang Chen, Priya Chudasama, Dario Callegaro, Dirk C. Strauss, Khin Thway, Alessandro Gronchi, Robin L. Jones, Rosalba Miceli, Sandro Pasquali, Paul H. Huang

“…Results DNA replication and cell cycle proteins were upregulated in high‐risk versus very low‐risk patients. Evaluation of the functional effects of CRISPR‐Cas9 gene knockdown of proteins enriched in high‐risk patients using the cancer cell line encyclopaedia database identified candidate drug targets. …”
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Interleukin‐11 promotes lung adenocarcinoma tumourigenesis and immune evasion by Cristina Cirauqui, Laura Ojeda, Itziar Otano, Irene Pazos, Alba Santos, Eva M. Garrido‐Martín, Patricia Yagüe, Javier Ramos‐Paradas, Sonia Molina‐Pinelo, Giovanna Roncador, José Luis Solórzano, M. Teresa Muñoz, Patricia Cozar, Patricia Plaza, Rocío Suárez, Marta Jiménez, Roberto Moreno, Arantxa Rosado, Pablo Gámez, Ricardo García‐Luján, Jon Zugazagoitia, E. Alejandro Sweet‐Cordero, Mariano Barbacid, Amancio Carnero, Irene Ferrer, Luis Paz‐Ares

“…Methods Here, we used transcriptomic and digital spatial profiling analyses together with clinic data from two retrospective LUAD patient cohorts. LUAD cell lines genetically engineered to overexpress or to silence IL‐11 or its receptor (IL‐11RA) were used for in vitro functional analysis and for in vivo experiments. …”
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Construction of mitochondrial signature (MS) for the prognosis of ovarian cancer by Miao Ao, You Wu, Kunyu Wang, Haixia Luo, Wei Mao, Anqi Zhao, Xiaomeng Su, Yan Song, Bin Li

“…After univariate Cox analysis, prognostic genes were carried out for modeling mitochondria signature (MS) based on 101 combinations of 10 machine learning algorithms. Functional enrichment analysis was performed on this prognostic gene set. …”
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Elevated prolactin levels before endometrial transformation negatively impact reproductive outcomes in frozen embryo transfer cycles under hormone replacement therapy by Yaxin Wang, Fei Sheng, Li Cao, Jiaying Ni, Meishan Wang, Yan Yang, Jiating Wang, Jingyi Lu, Jian Zhou, Hongfang Shao

“…Abstract Introduction Prolactin (PRL) plays a key role in the regulation of reproductive functions. However, its impact on outcomes in infertility women undergoing assisted reproductive technology remains unclear. …”
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Novel multi-omics analysis revealing metabolic heterogeneity of breast cancer cell and subsequent development of associated prognostic signature by Peng Zhang, Cuicui Li, Fen Li, Jiezhong Wu, Kunpeng Hu, He Huang

“…Drug sensitivity prediction was performed via the OncoPredict tool. Functional assays investigated the oncogenic role of PDCD1 in breast cancer cell lines. …”
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Arsenic trioxide regulates DYNAP through hsa-mir-573 and inhibits the proliferation of laryngeal cancer by Yanru Ren, Xiao Yang, Yang Hui, Weiyao Chen, Yi Cheng, Ning Zhang, Tao Liu, Xinxin Yang, Xiaoyu Li

“…ATO significantly inhibited LSCC cell proliferation in a dose- and time-dependent manner. miRNA-sequencing identified hsa-miR-573 as significantly upregulated following ATO treatment, and functional studies demonstrated that hsa-miR-573 suppresses LSCC cell proliferation by directly targeting DYNAP. …”
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Oral propranolol for treatment of the subgroups of essential tremor: a systematic review and meta-analysis protocol by Wei Li, Hui Shen, Tian Zhang, Li Chen, Manyu Zhang, Lan Hu, Liu Yang, Yanan Peng, Shijun Gao, Zhibin Chen, Tan Wang, Zhenqiang Zhao

“…Only oral drugs (propranolol, primidone, topiramate, etc）are still the first-line treatment recommended by the Food and Drug Administration. …”
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Collagen prolyl 4-hydroxylase subunit α member-induced head and neck squamous cell carcinoma aggressiveness is antagonized by LLGL2 via redu... by Xu Miao, Shi Run, Yang Jie, Chen Heng, Liu Shihua, Yu Shupei, Li Sasa, He Wenqiang, Sy Man-Sun, Lu Mingjian, Zhang Huixia, Li Chaoyang

“…Silencing of LLGL2 in the human oral squamous cell line Cal-27 upregulates the expression of occludin and increases cancer cell invasion and migration. …”
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1,4‐Dioxane Induces Epithelial‐Mesenchymal Transition and Carcinogenesis in an Nrf2‐Dependent Manner by Ziwei Wang, Chitra Thakur, Zhuoyue Bi, Yiran Qiu, Wenxuan Zhang, Haoyan Ji, Arjun K. Venkatesan, Sashank Cherukuri, Ke Jian Liu, John D. Haley, Xinwei Mao, Jaymie Meliker, Fei Chen

“…Collectively, our findings suggest that 1,4‐D is a human carcinogen, with its carcinogenicity largely dependent on Nrf2 activation, which orchestrates the biogenesis of EVs with EMT‐promoting functions.…”
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Novel human single-domain antibodies exert potent anti-tumor activity by targeting EGF-like repeat epitope of EpCAM by Xiaofeng Zhou, Zhifang Liu, Weixiong Zhang, Lin Dai, Tao Chen, Zexiong Lin, Hong Pan, Qi Qi, Henry Wei

“…The effects of these sdAbs on cell proliferation, migration, invasion, and apoptosis were tested in vitro, and their anti-tumor activity was assessed in a xenograft model.ResultsFive fully human anti-EpCAM sdAbs were isolated, all of which specifically bound to the EpCAM peptide and showed selective binding to various cancer cell lines, but not to 293T and 3T3 cells. Functional assays demonstrated that these sdAbs significantly inhibited cancer cell proliferation, migration, and invasion, and induced apoptosis. …”
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MEK inhibitor PD0325901 upregulates CD34 expression in endothelial cells via inhibition of ERK phosphorylation by Chihiro Hosoda, Seiji Mitani, Asuka Sakata, Shogo Kasuda, Yu Onodera, Yoko Takabayashi, Midori Shima, Kohei Tatsumi

“…However, the number and quality of CD34-positive cells decrease owing to various external and internal factors; thus, an efficient method is needed to establish CD34-positive EPCs. The generation of functional cells by reprogramming, that is, manipulating cell fate via gene transfer and/or treatment with chemical compounds, has recently been reported. …”
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Genome and transcriptome analysis of Chinese wheat powdery mildew isolate 21–2 for discovery of important virulence determinants by Fansong Zeng, Bo Zhang, Bin Yuan, Minfeng Xue, Wenqi Shi, Shuangjun Gong, Tom Hsiang, Dazhao Yu, Lijun Yang

“…This study provides comprehensive genomic and transcriptomic insights into Chinese Bgt isolate 21–2, and reveals virulence determinants and their variants fundamental to pathogenesis. Future functional analysis of such genes will enhance our understanding of pathogenic mechanisms of powdery mildews.…”
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TOM40 as a prognostic oncogene for oral squamous cell carcinoma prognosis by Lifei Deng, Hong Ran, Dunhui Yang, Zhen Wang, Peng Zhao, Hengjie Huang, Yongjin Wu, Peng Zhang

“…A network of genes co-expressed with TOM40 was constructed and functionally annotated by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. …”
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Natural pigments: innovative extraction technologies and their potential application in health and food industries by Ayu Masyita, Gemala Hardinasinta, Ayun Dwi Astuti, Firdayani Firdayani, Dian Mayasari, Aki Hori, Ira Nur Ainun Nisha, Firzan Nainu, Takayuki Kuraishi

“…For example, delphinidin (30 µM) significantly inhibited the growth of three cancer cell lines (B16-F10, EO771, and RM1) by at least 90% after 48 h. …”
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