NAMPT and NNMT released via extracellular vesicles and as soluble mediators are distinguished traits of BRAF inhibitor resistance of melanoma cells impacting on the tumor microenvi... by Beatrice Ghezzi, Irene Fiorilla, Ágata Carreira, Francesco Recco, Leonardo Sorci, Lidia Avalle, Alessia Ponzano, Francesca Mazzola, Alberto Maria Todesco, Nicoletta Tommasi, Massimiliano Gasparrini, Vito Giuseppe D’Agostino, Flavio Mignone, Alessandro Provenzani, Valentina Audrito

“…Lastly, we proposed for the first time a tetrameric NNMT:TLR4 binding model offering a plausible structural and mechanistic basis for their association. Our functional results indicated that exogenous NNMT treatment is able to trigger NF-κB pathway, one of the main TLR4-dependent signaling, sharing this cytokine-like properties with NAMPT, and opening a future deeper exploration of its functional role in the extracellular space. …”
Get full text

Identification and validation of a KRAS-macrophage-associated gene signature as prognostic biomarkers and potential therapeutic targets in melanoma by Beichen Cai, Beichen Cai, Beichen Cai, Qian Lin, Qian Lin, Qian Lin, Ruonan Ke, Ruonan Ke, Ruonan Ke, Jiaqi Yu, Jiaqi Yu, Jiaqi Yu, Lu Chen, Lu Chen, Xuejun Ni, Xuejun Ni, Hekun Liu, Xiuying Shan, Xiuying Shan, Biao Wang, Biao Wang, Biao Wang

“…Experimental validation of gene expression was performed via immunohistochemistry (IHC) and functional assays in gene-modified melanoma cell lines.ResultsTwenty-two genes involved in KRAS signaling were identified as critical for patient survival. …”
Get full text

Increased protein expression of interleukin-10 and its signalling molecules in colon cancer progression: potential prognostic and therapeutic targets by Akhmed Aslam, Bassem Refaat, Riyad A. Almaimani, Ahmad A. Obaid, Abdulrahman Mujalli, Wesam F. Farrash, Mohamed E. Elzubier, Shakir Idris, Afnan Salaka, Ahmed H. Almalki, Mofareh Y. Alkhaldi, Hassan A. Asiri, Mohammad A. Baqassi, Ali M. Alhanash, Othman M. Hakmi, Faisal Minshawi

“…The data were then stratified according to clinical stages (early-stage I/II vs. late-stage III/IV) and tumour sites (right-sided cancers; RSCs vs. left-sided cancers; LSCs). Functional effects of biologically active IL-10 protein (0.1, 1, and 40 ng/ml), anti-IL10Rα monoclonal antibody (0.1, 1, and 40 ng/ml), and a single concentration of a specific STAT3 inhibitor (2 µM) on cell cycle and apoptosis were assessed in HT29 and SW620 CRC cell lines, along with the expression of key regulatory molecules. …”
Get full text

ALOXE3 expression predicts poor prognosis and modulates immune infiltration in colon adenocarcinoma by Liwen Zhao, Liya Zhao, Siyu Ye, Shengnan Jing, Han Yang, Yixin Qi, Zhaofeng Li, Zaiqing Jiang, Xuechuan Yan, Ke Wang, Yong-Jing Gao, Tianzhen He

“…Additionally, immune cell infiltration associated with LOX expression was explored using the TIMER and TISIDB databases. For functional validation, ALOXE3 was either overexpressed or silenced via shRNA in colon cancer cell lines, and its effects on tumor progression were assessed through in vitro proliferation assays and in vivo xenograft models. …”
Get full text

Disruption of glutamine transport uncouples the NUPR1 stress-adaptation program and induces prostate cancer radiosensitivity by Uğur Kahya, Vasyl Lukiyanchuk, Ielizaveta Gorodetska, Matthias M. Weigel, Ayşe Sedef Köseer, Berke Alkan, Dragana Savic, Annett Linge, Steffen Löck, Mirko Peitzsch, Ira-Ida Skvortsova, Mechthild Krause, Anna Dubrovska

“…The level of gene expression was analyzed by RNA sequencing and RT-qPCR in the established cell lines or patient-derived tumor and adjacent non-cancerous tissues. …”
Get full text

S1PR4-dependent effects of Etrasimod on primary human myeloid immune cell activation by Fiona K. Sailer, Megan A. Palmer, Blerina Aliraj, Jan Heering, Jan Heering, Andreas Brockmann, Mohammed A. F. Elewa, Mohammed A. F. Elewa, Aissa Röhrig, Aissa Röhrig, Ewgenij Proschak, Ewgenij Proschak, Ewgenij Proschak, Dariusz T. Stepniak, Simeon Ramsey, Bernhard Brüne, Bernhard Brüne, Andreas Weigert

“…Regarding receptor dynamics, we show that Etrasimod induces internalization of S1PR4.ConclusionTaken together, our data show that S1PR4 takes on an essential role in the regulation of various immunological functions, and that Etrasimod can act as a superagonist/functional antagonist of S1PR4.…”
Get full text

Targeting TUBB2B inhibits triple-negative breast cancer growth and brain-metastatic colonization by Qingling He, Jianyang Hu, Fung-Yin Ngo, Huiqi Zhang, Lin He, Hao Huang, Tan Wu, Yilin Pan, Zihan Yang, Yuanyuan Jiang, William C. Cho, Wah Cheuk, Gary M. Tse, Julia Y. Tsang, Mengsu Yang, Liang Zhang, Xin Wang, Pui-Chi Lo, C. Geoffrey Lau, Y. Rebecca Chin

“…Mechanistically, we identify eukaryotic translation elongation factor 1 alpha 1 (eEF1A1) as a novel interacting partner of TUBB2B, revealing a previously unexplored role of TUBB2B in translational regulation. In line with its neural-related functions, TUBB2B overexpression in TNBC cells activates astrocytes, which in turn upregulate TUBB2B in tumor cells. …”
Get full text

Integrated system for screening tumor-specific TCRs, epitopes, and HLA subtypes using single-cell sequencing data by Yi Li, Weiguo Zhang, Xuelian Song, Xianyao Wang, Yanshu Ding, Chenfei Yin, Tianyuan Ren

“…By using this system, we have successfully identified a functional, HPV-specific TCR, demonstrating the potential of our approach for the efficient screening of tumor-specific TCRs to advance TCR-T immunotherapy.…”
Get full text

Generation of non-genetically modified, CAR-like, NK cells by Martine Pugnière, Bruno Robert, Pierre Martineau, Loïs Coënon, Jessy Présumey, Emilie Rigal, Hortense Courot, Caroline Multrier, Sara Zemiti, Jennifer Lambour, Marion de Toledo, Guillaume Bossis, Guillaume Cartron, Bénédicte Fauvel, Martin Villalba

“…We have generated the new technology “Pin”, which enables the arming of modified monoclonal antibodies (mAbs) onto the CD16a of ex vivo expanded NK (eNK) cells.MethodsEx vivo eNK were prepared from umbilical cord blood cells and expanded using interleukin (IL)-2/IL-15 and Epstein-Barr virus (EBV)-transformed B-lymphoblastoid feeder cells. mAbs were engineered with four substitutions called Pin mutations to increase their affinity to CD16a. eNK were incubated with anti-CD20 or anti-CD19 Pin-mAbs to generate “armed” eNK and were used to assess effector functions in vitro on cancer cell lines, lymphoma patient cells and in vivo.Results CD16a/Pin-mAb interaction is stable for several days and Pin-mAb eNK inherit the mAb specificity and exclusively induce ADCC against targets expressing the cognate antigen. …”
Get full text

Integrative analysis of T cell-associated markers in Ewing sarcoma reveals prognostic signatures and immune dynamics by Umair Ali Khan Saddozai, Umair Ali Khan Saddozai, Chenxu Liu, Chenxu Liu, Fei Yan, Zhendong Lu, Muhammad Babar Khawar, Muhammad Babar Khawar, Muhammad Usman Akbar, Saadullah Khattak, Haibo Sun, Haibo Sun, Ping Yang

“…Differentially expressed genes (DEGs) intersected with T cell markers identified a total of 174 T cell-associated genes. Functional enrichment analysis and molecular subtyping were performed to explore immune-related pathways. …”
Get full text

Long noncoding RNA LINC00339 promotes osteoporosis development via modulating of regulator CDC42 by binding PARP1 by Dong-Li Zhu, Yan Zhang, Xiao-Yu Zhang, Zi-Han Qiu, Ke Li, Xiao-Rong Zhou, Zhen-Zhen He, Xiao-Feng Chen, Shan-Shan Dong, Wen Tian, Ya-Kang Wang, Tie-Lin Yang, Bo Yang, Yan Guo

“…Our previous investigation provided preliminary evidence that single nucleotide polymorphisms (SNPs) may functionally interact with the LINC00339, potentially contributing to the pathogenesis and progression of osteoporosis through undefined molecular pathways. …”
Get full text

Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice by Anindya Ghosh, Isabelle Chénier, Yat Hei Leung, Abel K. Oppong, Marie-Line Peyot, S. R. Murthy Madiraju, Irina Al-Khairi, Jehad Abubaker, Fahd Al-Mulla, Marc Prentki, Mohamed Abu-Farha

Get full text

Factors contributing to increased Neonatal Mortality in Grand Kru County, Liberia, January 2017-December 2021 by Jimmy Lawubah, Chukwuma David Umeokonkwo, Himiede Wede Sesay, Babalola Joseph Obafemi, Faith Tina Whesseh, Leroy Maximore, Francis Bobway, Boye Nuyelleh, Lily Marie Sanvee-Blebo, Maame Amo-Addae

“…Birth asphyxia and neonatal sepsis were the leading causes of neonatal deaths, while late community referral, lack of functional incubators and delayed ambulance services for patients' referral were leading factors contributing to neonatal mortality in Grand Kru County.…”
Get full text

Blockchain Research and Development Activities Sponsored by the U.S. Department of Energy and Utility Sector by Sydni Credle, Nor Farida Harun, Grant Johnson, Jeremy Lawrence, Christina Lawson, Jason Hollern, Mayank Malik, Sri Nikhil Gupta Gourisetti, D. Jonathan Sebastian-Cardenas, Beverly E. Johnson, Tony Markel, David Tucker

“…The second dimension, <i>Blockchain Properties</i>, captures the specific needs and functionalities provided by Blockchain technology. The aim was to capture blockchain’s applicability and functionality: where and why blockchain? …”
Get full text

Elaboración, validez de contenido y fiabilidad del instrumento de observación SOCTOD-BSR para el análi-sis de acciones en baloncesto en silla de ruedas (Development, content validi... by Adrián García-Fresneda, Verónica Muñoz-Arroyave, Xavier Iglesias, Apostolos Theodorou, David Suárez Iglesias, Alba Pardo Fernández

“…Keywords: psychometric properties, observational methodology, functional classification, line-up formations, parasport. …”
Get full text

PO80 | Heparin-induced thrombocytopenia (HIT): a monocentric experience with danaparoid

“…Aims and Methods: We report five HIT cases diagnosed with 4T score ≥4, positive immunologic anti-PF4 IgG (HemosIL Acustar), and positive functional test (P-selectin expression by flow cytometry). …”
Get full text

PYK2 promotes cell proliferation and epithelial-mesenchymal transition in endometriosis by phosphorylating Snail1 by Lu Liu, Lan Liu, Chenjing Yue, Shiyu Du, Jiayu Liu, Zhenhai Yu

“…Protein extracts from both ectopic tissues and cells were subjected to Western blot and co-immunoprecipitation (Co-IP) interaction validation. Functional assays (proliferation/migration/invasion) were performed using EESC and 11Z cell lines with triplicate biological replicates. …”
Get full text

Comparison of human-induced pluripotent stem cell-derived macrophages with peripheral blood-derived macrophages using single-cell genomics by ZHANG Yutong, HOU Guojun, SHEN Nan

“…GO analysis of DEGs showed enrichment in the molecular functions such as ubiquitin-like protein ligase binding, cellular components such as the nuclear speck and nuclear envelope, and biological processes such as the regulation of translation. …”
Get full text

Pathohistological properties and histomorphometric characteristics of cancellous bone tissue in iliobiopsies of patients with osteogenesis imperfecta by V. V. Grigorovsky, A. M. Zyma

“…In OI-patients trabecular bone tissue had the complex pattern formed by basophylic cemental lines which outlined interstitial microstructures of lamellar and woven kind. …”
Get full text

Phenolic supplements: testing an approach to limit Sphagnum decomposition in a Sphagnum farming system by Talal Asif, Line Rochefort, Chris Freeman, Christian Dunn, Hojeong Kang, Mélina Guêné-Nanchen

Get full text