CLIC2 regulates immunosuppression and macrophage differentiation in genomically stable gastric cancer by Viviana Longo, Pellegrino Mazzone, Giovanni Calice, Pietro Zoppoli, Giuseppina Di Paola, Giuseppe Cesta, Margherita Luongo, Claudia Sabato, Sabino Russi, Simona Laurino, Tiziana Notarangelo, Giuseppe Patitucci, Chiara Balzamo, Valeria Lucci, Elena Amendola, Giuseppina Amodio, Paolo Remondelli, Valentina Pagliara, Maria Rita Milone, Roberta Guadagno, Cristofaro De Stefano, Ferdinando De Vita, Geppino Falco, Francesco Albano

“…Abstract Chloride intracellular channels (CLICs) are a family of six evolutionarily conserved proteins with diverse functions. Previously, we identified CLIC2, as the fifth-ranked master regulator associated with diffuse-type gastric cancer (dGC) showing increased expression in tumors. …”
Get full text

Elevated THOC5 expression in liver cancer and its implications for tumor progression and therapeutic response by Jin-Shu Pang, Jin-Shu Pang, Yu Yang, Yu Yang, Li Liang, Yu-Jia Zhao, Jin-Dan Li, Li-Peng Li, Xue-Xin Wei, Rui-Zhi Gao, Rong Wen, Yun He, Hong Yang, Hong Yang, Hong Yang, Xiu-Mei Bai, Xiu-Mei Bai

“…Single-cell RNA sequencing, in-house RNA-seq, immunohistochemistry, and proteomic analysis were employed to further validate THOC5 expression and prognosis in LIHC. Functional assays, including wound-healing and Transwell migration, were performed following THOC5 knockdown in LIHC cell lines. …”
Get full text

ROS-responsive drug delivery system with enhanced anti-angiogenic and anti-inflammatory properties for neovascular age-related macular degeneration therapy by Jiayi Zuo, Yining Pan, Yuanli Wang, Wei Wang, Haojie Zhang, Si Zhang, Youru Wu, Jiangfan Chen, Qingqing Yao

“…Therefore, combinational therapeutics with anti-angiogenic, reactive oxygen species (ROS)-scavenging and anti-inflammatory functions will broaden therapeutic effects and reduce side effects. …”
Get full text

Demethylzeylasteral inhibits proliferation and metastasis of osteosarcoma cells by modulating the PI3K/AKT/Autophagy pathways by Xuhui Yuan, Jiayu Li, Bo Yu, Feng Cai, Binqi Chen, Jun Liu, Yuanxiang Peng, Duo Zeng, Qi Liao, Lang Liu

“…Purpose: This study focused on the anti-tumor properties of DEM on OS cells as well as the potential mechanisms. Methods: OS cell lines (MG63 and 143B) were exposed to varying concentrations of DEM, followed by assessment of diverse cell functions. …”
Get full text

Integrated spatial and single cell transcriptomics identifies PRKDC as a dual prognostic biomarker and therapeutic target in hepatocellular carcinoma by Wenpeng Pang, Yunyong Wang, Xiaohang Lu, Minpeng Li, Fuli Long, Songlin Chen, Yuan Yu, Mingfen Li, Hongsheng Lin

“…Copy number variation (CNV) analysis revealed significant genomic instability, with PRKDC exhibiting both amplifications and deletions across chromosomal regions, underscoring its role in tumorigenesis. Functional overexpression of PRKDC in HCC cell lines enhanced cell proliferation, migration, and altered cell cycle dynamics, with a notable increase in the G2/S phase. …”
Get full text

Transcriptomic Profiling Reveals the Involvement of the Phenylpropanoid–Lignin Pathway in the Response of Maize Roots to Zinc Stress by Ying Zhou, Tianyu Gu, Yan Gao, Jingtao Qu, Hongjian Zheng, Yuan Guan, Jiashi Peng

“…Zinc (Zn) is an essential micronutrient required for plants to perform various metabolic functions, and plant responses to Zn deficiency have been extensively studied. …”
Get full text

Predicting hepatocellular carcinoma outcomes and immune therapy response with ATP-dependent chromatin remodeling-related genes, highlighting MORF4L1 as a promising target by Chao Xu, Litao Liang, Guoqing Liu, Yanzhi Feng, Bin Xu, Deming Zhu, Wenbo Jia, Jinyi Wang, Wenhu Zhao, Xiangyu Ling, Yongping Zhou, Wenzhou Ding, Lianbao Kong

“…Experimental validation was conducted using quantitative real-time Polymerase Chain Reaction (qRT-PCR), Western blotting, and functional assays in HCC cell lines and xenograft models. …”
Get full text

PAQR5 drives the malignant progression and shapes the immunosuppressive microenvironment of hepatocellular carcinoma by activating the NF-κB signaling by Ruida Yang, Huanhuan Wang, Cong Wu, Yu Shi, Hanqi Li, Xinyue Bao, Yuqian Yang, Shaoshan Han, Xue Yang, Jie Tao, Hao Sun, Shaobo Wu, Liankang Sun

“…Utilizing data from bulk, single-cell, and spatial transcriptomics cohorts, this study systematically assessed the expression patterns, immune landscape, and functional characteristics of PAQR5 across different levels of resolution in HCC. …”
Get full text

Developing transcriptomic biomarkers for TAVO412 utilizing next generation sequencing analyses of preclinical tumor models by Ying Jin, Peng Chen, Huajun Zhou, Guangmao Mu, Simin Wu, Zhengxia Zha, Bin Ma, Chao Han, Mark L. Chiu, Mark L. Chiu

“…TAVO412 has multiple mechanisms of action for tumor growth inhibition that include shutting down the EGFR, c-Met, and VEGF signaling pathways, having enhanced Fc effector functions, addressing drug resistance that can be mediated by the crosstalk amongst these three targets, as well as inhibiting angiogenesis. …”
Get full text

Age-linked DNA methylation and gene expression patterns in parameningeal head and neck alveolar rhabdomyosarcoma reveal CDK9 as a promising therapeutic target by Sara Patrizi, Silvia Vallese, Sabina Barresi, Matteo Cassandri, Isabella Giovannoni, Lucia Pedace, Luana Abballe, Flavia Vinciarelli, Celeste Antonacci, Alessandra Stracuzzi, Barbara Mancini, Ida Russo, Angela Di Giannatale, Rossella Rota, Rita Alaggio, Franco Locatelli, Giuseppe Maria Milano, Evelina Miele

“…We validated the biological relevance of molecules of interest through functional experiments on rhabdomyosarcoma cell lines. …”
Get full text

Anti-colorectal cancer effect of total minor ginsenosides produced by lactobacilli transformation of major ginsenosides by inducing apoptosis and regulating gut microbiota by Yunjiao Shen, Yunjiao Shen, Yansong Gao, Ge Yang, Zijian Zhao, Yujuan Zhao, Lei Gao, Shengyu Li

“…Total minor ginsenosides (TMG) were obtained through the fermentation of major ginsenosides with Lactiplantibacillus plantarum, and potential anticancer effects of TMGs on the mouse colon cancer cell line CT26.WT, in vitro and in vivo, were investigated.Materials and MethodsWe employed the Cell Counting Kit-8 (CCK-8), TdT-mediated dUTP nick end labeling (TUNEL), and Western blot analysis in vitro to explore the anti-proliferative and pro-apoptotic functions of TMG in CT26.WT cells. …”
Get full text

GLUT3 enhances chemosensitivity in glioblastoma by transporting temozolomide and capecitabine by Honglin Diao, Yuxin Sun, Xiaojia Zhou, Qikai Wang, Mingyue Wang, Keyu Chen, Zhihua Huang, Jianlei Wei, Zeping Li, Yaxin Lou, Zebin Mao, Wenhua Yu

“…Temozolomide (TMZ), the sole first-line chemotherapeutic agent for GBM, faces substantial challenges in overcoming this resistance. …”
Get full text

Cytotoxic Activity and Senescence Modulatory Effect of Hesperetin on Triple-Negative Breast Cancer Cells and Kidney Cells Co-Treatment with Cisplatin by Anif Nur Artanti, Riris Istighfari Jenie, Rumiyati Rumiyati, Edy Meiyanto

“…Hesperetin (HST), a flavanone found in various citrus fruits, exhibits bioactive properties, functioning as an antioxidant, anti-inflammatory, and anticancer agent. …”
Get full text

Decoding the mannose receptor-mAb interaction: the importance of high-mannose N-glycans and glycan-pairing by Julia Baumeister, Maximilian Meudt, Sybille Ebert, Frank Rosenau, Boris Mizaikoff, Michaela Blech, Kristina M. J. Aertker, Fabian Higel

“…During the development process of therapeutic monoclonal antibodies (mAbs), it is crucial to control (critical) quality attributes such as N-glycosylation influencing pharmacokinetics (PK) and Fc effector functions. Previous reports have shown that mAbs containing high-mannose N-glycans are cleared faster from blood circulation, leading to reduced half-lives. …”
Get full text

The effect of chronic, non-specific low back pain on superficial lumbar muscle activity: a protocol for a systematic review and meta-analysis by Alison B Rushton, Nicola R Heneghan, Deborah Falla, Andy Sanderson, Eduardo Martinez Valdes, Alessio Gallina

“…Several studies have investigated this activity using surface electromyography, in varied muscles and during a number of functional activities. Due to differences in the applied methodology, the results have been difficult to compare, and previous reviews have been limited in scope. …”
Get full text

High-quality bonds: serine acetyltransferase 2 gene revealed by GWAS is associated with grain protein content in spring durum wheat by Aleksey Ermolaev, Ludmila Bespalova, Varvara Korobkova, Aleksey Yanovsky, Lubov Nazarova, Aleksandra Kroupina, Anastasia Chernook, Aleksandra Mudrova, Viktoria Voronezhskaya, Viktoria Voronezhskaya, Pavel Kroupin, Gennady Karlov, Mikhail Divashuk, Mikhail Divashuk

“…Through the analysis of linkage disequilibrium decay rate, and functional gene ontology annotation, the serine acetyltransferase 2 gene involved in cysteine biosynthesis was identified as a candidate gene for GPC. …”
Get full text

ANP32E expression in pancreatic cancer is associated with impaired gemcitabine efficacy and poor patient prognosis by Xiaohong Liu, Yelin Zhao, Li Zhang, Junting Wang, Liaoxin Luo, Shihui Zhang, Qin Zhu, Yuchen Shi, Chenyu Yuan, Qifeng Xiao, Mengran Xiong, Yuanyuan Duan, Hebing Chen, Hongjuan Yao, Lin Cai, Jianwei Zhang, Guangxi Li, Liang Li

“…Methods: We collected clinical characteristics and treatment information from a total of 75 PDAC patients to assess the association of ANP32E expression via immunohistochemical (IHC) staining with overall survival (OS) in patients who were or were not treated with gemcitabine-based chemotherapy, followed by a clinical replication study with transcriptomic data from the TCGA database and functional validation experiments involving the knockdown of ANP32E in the Hup-T3 and SU86.86 human pancreatic cancer cell lines. …”
Get full text

The contribution of the CRP/CD64 axis to renal cancer progression by inducing protumor activation of tumor‐associated macrophages by Cheng Pan, Yukio Fujiwara, Hiromu Yano, Toshiki Anami, Yuki Ibe, Lianbo Li, Yuji Miura, Takanobu Motoshima, Shigeyuki Esumi, Junji Yatsuda, Taizo Hibi, Tomomi Kamba, Yoshihiro Komohara

“…Results A co‐culture study using human macrophages and RCC cell lines showed that CRP‐stimulated macrophages secrete IL‐6, which induces RCC proliferation via STAT3 activation. …”
Get full text

Advances in the study of artemisinin and its derivatives for the treatment of rheumatic skeletal disorders, autoimmune inflammatory diseases, and autoimmune disorders: a comprehens... by Zhiyong Long, Wang Xiang, Wei Xiao, Yu Min, Fei Qu, Bolin Zhang, Liuting Zeng

“…Artemisinin and its derivatives are widely recognized as first-line treatments for malaria worldwide. Recent studies have demonstrated that artemisinin-based antimalarial drugs, such as artesunate, dihydroartemisinin, and artemether, not only possess excellent antimalarial properties but also exhibit antitumor, antifungal, and immunomodulatory effects. …”
Get full text

Allelic Variation of Hd17 for Rice Heading Date is Caused by Natural Selection by Zifeng Yang, Yun Li, Jin Liu, Shuiqing Wu, Xuelin Wang, Min Guan, Yanyun Li, Haitao Zhu, Guifu Liu, Shaokui Wang, Guiquan Zhang

“…The Hd17-2 allele was caused by the functional nucleotide polymorphism (FNP) of C to T at position 1016 of the gene and exhibits delay heading. …”
Get full text