Integrating GPT-Based AI into Virtual Patients to Facilitate Communication Training Among Medical First Responders: Usability Study of Mixed Reality Simulation by Rodrigo Gutiérrez Maquilón, Jakob Uhl, Helmut Schrom-Feiertag, Manfred Tscheligi

“…MethodsWe developed an MR simulation of a traffic accident with a VP. ChatGPT (OpenAI) was integrated into the VP and prompted with verified characteristics of accident victims. …”
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Integrative single-cell and spatial transcriptomics uncover ELK4-mediated mechanisms in NDUFAB1+ tumor cells driving gastric cancer progression, metabolic reprogramming, and immune... by Yuwei Sun, Wenyang Nie, Zhikai Xiahou, Xiaojing Wang, Wenjia Liu, Zongkai Liu, Zhiheng Lin, Zhaidong Liu

“…Cell type identification was accomplished using the tool of Seurat, and the spatial distribution of cell types was revealed through the Robust Cell Type Decomposition technique. CellChat was used to analyze the interactions between key cell subtypes and other cells, and the “StLearn” package was employed to investigate spatial cell communication in depth. …”
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scRNA-Seq reveals age-dependent microglial evolution as a determinant of immune response following spinal cord injury by Lufei Shao, Yueliang Chang, Jinfang Liu, Leilei Lin, Long Chang, Jialin Zhang, Zhibin Lan, Honglai Zhang, Xiaolei Chen

“…Spinal cord injury (SCI) is a debilitating condition of the central nervous system (CNS) that leads to severe impairments in sensory and motor functions. Previous studies have pointed out that patient age is a critical factor influencing SCI prognosis. …”
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Digital augmentation of aftercare for patients with anorexia nervosa: the TRIANGLE RCT and economic evaluation by Janet Treasure, Katie Rowlands, Valentina Cardi, Suman Ambwani, David McDaid, Jodie Lord, Danielle Clark Bryan, Pamela Macdonald, Eva Bonin, Ulrike Schmidt, Jon Arcelus, Amy Harrison, Sabine Landau

“…Carers were invited to a biweekly chat group which followed a similar format to the groups above. …”
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Decoding the hypoxic tumor microenvironment in colorectal cancer for prognostic modeling and therapeutic target discovery by Xiao-Cui Duan, Yan Zhou, Fan Feng, Hai-Bo Jiang, Mei-Lin Wang, Zhe Han, Hong-Fei Pang, Yu-Hang Liu, Heng-Zhe Jia, Meng- He, Hong-Pan Xu, Yuan-Yuan Wang

“…Cell–cell communication networks were inferred using CellChat, and transcription factor regulatory modules were reconstructed via SCENIC and GRNBoost2. …”
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TSPAN4+ fibroblasts coordinate metastatic niche assembly through migrasome-driven metabolic reprogramming and stromal-immune crosstalk in pancreatic adenocarcinoma by Qingwen Hu, Qingwen Hu, Jiali Chen, Yang Liu, Yang Liu, Haiqing Chen, Haotian Lai, Lai Jiang, Xuancheng Zhou, Shengke Zhang, Jinbang Huang, Hao Chi, Bo Li, Xiaolin Zhong

“…Cell communication was assessed using CellCall and CellChat. Spatial transcriptomic analysis was conducted with RCTD and MISTy tools to investigate cell interactions within the TME. …”
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Large language models meet user interfaces: The case of provisioning feedback by Stanislav Pozdniakov, Jonathan Brazil, Solmaz Abdi, Aneesha Bakharia, Shazia Sadiq, Dragan Gašević, Paul Denny, Hassan Khosravi

“…A significant portion of the current use of LLMs by educators has involved using conversational user interfaces (CUIs), such as chat windows, for functions like generating educational materials or offering feedback to learners. …”
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Fall risk perception toolkit based on “digital human” for community-dwelling older patients with stroke: development and validation by Jiang Nan, Yi Hou, Manyao Sun, Xueying Huang, Jing Gao, Xueqiong Zou, Zhongyi Chen, Yuyu Jiang

“…Moreover, the design of the WeChat mini program increased its generalizability to promote more successful health care delivery. …”
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Harnessing single-cell and multi-omics insights: STING pathway-based predictive signature for immunotherapy response in lung adenocarcinoma by Yang Ding, Dingli Wang, Dali Yan, Jun Fan, Zongli Ding, Lei Xue

“…Advanced bioinformatics analyses using R packages (Seurat, CellChat) revealed transcriptomic heterogeneity, intercellular communication networks, and immune landscape characteristics. …”
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Multi-omics analyses of the heterogenous immune microenvironment in triple-negative breast cancer implicate UQCRFS1 potentiates tumor progression by Yuhui Tang, Aiqi Xu, Zhongbiao Xu, Jindong Xie, Wei Huang, Liulu Zhang, Yitian Chen, Lu Yang, Shasha Du, Kun Wang

“…Methods The thorough analyses of different cell types within TME were conducted on the self-tested single-cell RNA sequencing dataset which contained nine TNBC treatment-naïve patients, including subclusters classification, CellChat algorithm, transcription factors (TFs) expression, pseudotime analysis and functional enrichment assay. …”
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Single‐cell RNA‐sequencing and spatial transcriptomic analysis reveal a distinct population of APOE− cells yielding pathological lymph node metastasis in papillary thyroid cancer... by Guohui Xiao, Rongli Xie, Jianhua Gu, Yishu Huang, Min Ding, Dongjie Shen, Jiqi Yan, Jianming Yuan, Qiong Yang, Wen He, Siyu Xiao, Haizhen Chen, Dan Xu, Jian Wu, Jian Fei

“…Pseudotime analysis and CellChat were performed to explore the the molecular mechanisms of the APOE in PTC progression. …”
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Multidimensional transcriptomics based to illuminate the mechanisms of taurine metabolism in immune resistance of pancreatic cancer by Zongshuai Qin, Zongshuai Qin, Guixiang Huang, Guixiang Huang, Jian Xu, Jian Xu, Lujuan Pan, Chaojun Lan, Chaojun Lan, Yuhuan Yang, Yuhuan Yang, Yixia Yin, Yueqiu Qin, Yueqiu Qin, Yueqiu Qin

“…The metabolic process of taurine has emerged as a crucial factor in modulating the functions and activities of immune cells. Intervening in taurine metabolism holds the potential to reshape the tumor immune microenvironment, thereby enhancing the ability of immune cells to recognize and eliminate tumor cells. …”
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Single-cell and spatial atlas of glioblastoma heterogeneity: characterizing the PCLAF+ subtype and YEATS4’s oncogenic role by Siqi Ma, Yuwei Sun, Shaowei Zheng, Yilong Fu, Liangyu Wang, Dun Liu, Henan Jiao, Xuqiang Zhu, Xueyuan Li, Dongming Yan, Di Chen, Zi Ye

“…Spatial transcriptomics integrated Multimodal Intersection Analysis, TransferData, and Robust Cell Type Decomposition techniques to characterize the spatial distribution patterns of key cell subtypes. CellChat was employed to assess intercellular communication networks. …”
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Preliminary evaluation of a gamified smartphone intervention (O2O-PEP) for enhancing HIV post-exposure prophylaxis uptake in men who have sex with men: pilot feasibility study by Tianyu Cui, Tianying Li, Luyao Gong, Kexiang Wang, Qianqian Luo

“…To address this issue, we developed an innovative, gamified mini-app embedded in WeChat, known as O2O-PEP (online-to-offline collaborative model for HIV postexposure prophylaxis), that targets PEP uptake. …”
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A scRNA-seq reference contrasting living and early post-mortem human retina across diverse donor states by Luning Yang, Yiwen Tao, Qi Pan, Tengda Cai, Yunyan Ye, Jianhui Liu, Yang Zhou, Yongqing Shao, Quanyong Yi, Zen Huat Lu, Lie Chen, Gareth McKay, Richard Rankin, Weihua Meng

“…Transcriptional dynamics were assessed using RNA velocity, and cell-cell signaling pathways were inferred with CellChat. Key findings were validated in independent samples from two additional donors (four samples) using the identical workflow. …”
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Left atrial single-cell transcriptomics reveals amphiregulin as a surrogate marker for atrial fibrillation by Yuya Suzuki, Takuo Emoto, Shunsuke Sato, Takeshi Yoshida, Mitsuhiko Shoda, Hiromi Endoh, Manabu Nagao, Tomoyo Hamana, Taishi Inoue, Tomohiro Hayashi, Eriko Nitta, Hiroki Konishi, Kunihiko Kiuchi, Mitsuru Takami, Kimitake Imamura, Masayuki Taniguchi, Masatoshi Inoue, Toshihiro Nakamura, Yusuke Sonoda, Hiroyuki Takahara, Kazutaka Nakasone, Kyoko Yamamoto, Kenichi Tani, Hidehiro Iwai, Yusuke Nakanishi, Shogo Yonehara, Atsushi Murakami, Ryuji Toh, Takenao Ohkawa, Tomoyuki Furuyashiki, Ryo Nitta, Tomoya Yamashita, Ken-ichi Hirata, Koji Fukuzawa

“…Myeloid cells in LA tissue are categorized into five macrophage clusters, three monocyte clusters, and others. Cell-Chat analysis revealed that monocytes and IL1B+ macrophages send epidermal growth factor (EGF) signals to fibroblasts. …”
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TINJAUAN ASPEK KELAYAKAN ELEMEN PEMBENTUK RUANG KOMUNAL DI TAMAN MONUMEN 45 KOTA PEKALONGAN by Moch Fathoni Setiawan, Andi Purnomo

“…The hard elements are referred to support the communal activity for the citizens of Pekalongan. They are functioned as for gathering and chatting, lounging, skateboarding and BMX styling. …”
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“Dictionary of immune responses” reveals the critical role of monocytes and the core target IRF7 in intervertebral disc degeneration by Peichuan Xu, Peichuan Xu, Kaihui Li, Jinghong Yuan, Jinghong Yuan, Jiangminghao Zhao, Jiangminghao Zhao, Huajun Pan, Huajun Pan, Chongzhi Pan, Chongzhi Pan, Wei Xiong, Wei Xiong, Jianye Tan, Jianye Tan, Tao Li, Tao Li, Guanfeng Huang, Guanfeng Huang, Xiaolong Chen, Xiaolong Chen, Xinxin Miao, Dingwen He, Xigao Cheng, Xigao Cheng

“…Subsequently, Immune Response Enrichment Analysis (IREA) was conducted on the key immune cells to determine their cytokine responses and polarisation states in IDD. “CellChat” package facilitated the analysis of cell-cell communication. …”
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The role and use of speech gestures in discourse by Nick CAMPBELL

“…The technology exists to recognise these discourse events, but we still lack a model of their function in the mutual transfer of information through speech interaction.…”
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Identification and validation of a KRAS-macrophage-associated gene signature as prognostic biomarkers and potential therapeutic targets in melanoma by Beichen Cai, Beichen Cai, Beichen Cai, Qian Lin, Qian Lin, Qian Lin, Ruonan Ke, Ruonan Ke, Ruonan Ke, Jiaqi Yu, Jiaqi Yu, Jiaqi Yu, Lu Chen, Lu Chen, Xuejun Ni, Xuejun Ni, Hekun Liu, Xiuying Shan, Xiuying Shan, Biao Wang, Biao Wang, Biao Wang

“…Cell-cell communication within the SKCM microenvironment was explored using the “CellChat” tool. Experimental validation of gene expression was performed via immunohistochemistry (IHC) and functional assays in gene-modified melanoma cell lines.ResultsTwenty-two genes involved in KRAS signaling were identified as critical for patient survival. …”
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