EfficientNetB0-Based End-to-End Diagnostic System for Diabetic Retinopathy Grading and Macular Edema Detection by Long X, Gan F, Fan H, Qin W, Li X, Ma R, Wang L, Hu R, Xie Y, Chen L, Cao J, Shao Y, Liu K, You Z

“…Xin Long,1– 5,* Fan Gan,1– 4,* Huimin Fan,1– 4,* WeiGuo Qin,6 Xiaonan Li,1– 4 Rui Ma,1 Leran Wang,1 Rui Hu,1 Yilin Xie,1 Lei Chen,7 Jian Cao,1– 4 Yinan Shao,1– 4 Kangcheng Liu,1– 4 Zhipeng You1– 4 1Department of Fundus Diseases, The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330000, People’s Republic of China; 2Jiangxi Province Division of National Clinical Research Center for Ocular Diseases, Nanchang, Jiangxi, 330000, People’s Republic of China; 3Jiangxi Clinical Research Center for Ophthalmic Disease, Nanchang, Jiangxi, 330000, People’s Republic of China; 4Jiangxi Research Institute of Ophthalmology and Visual Science, Nanchang, Jiangxi, 330000, People’s Republic of China; 5School of Mathematics and Computer Sciences, Nanchang University, Nanchang, Jiangxi, 330000, People’s Republic of China; 6Department of Cardiothoracic Surgery, The 908th Hospital of Chinese People’s Liberation Army Joint Logistic Support Force, Nanchang, Jiangxi, 330001, People’s Republic of China; 7The Second Clinical School of Medicine, Nanchang University, Nanchang, Jiangxi, 330031, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhipeng You, The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, Jiangxi, 330006, People’s Republic of China, Tel +86 13970032654, Email yzp74@sina.comPurpose: This study aims to develop and validate a deep learning-based automated diagnostic system that utilizes fluorescein angiography (FFA) images for the rapid and accurate diagnosis of diabetic retinopathy (DR) and its complications.Methods: We collected 19,031 FFA images from 2753 patients between June 2017 and March 2024 to construct and evaluate our analytical framework. …”
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Effect of Wuzhuyu Decoction on alcoholic gastric ulcer and its mechanism by GAO Ruiyang, LI Jingyao, LI Pingxiang, GE Keli

“…Objective To investigate the effect of Wuzhuyu Decoction (WZYD) on alcoholic gastric ulcer and its mechanism. Methods A total of 120 female KM mice, aged 8 weeks, were randomly divided into normal control group (group A), model control group (group B), ranitidine group (group C), and low-, middle-, and high-dose WZYD groups (groups D-F), with 20 mice in each group. …”
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Understanding the Needs and Wishes of Older Adults in Interprofessional Treatment for Malnutrition and Sarcopenia: A Grounded Theory Study by Boxum SD, Van Exter SH, Reinders JJ, Drenth H, Van den Berg MG, Tieland M, Geluk-Bleumink A, Spoorenberg SL, Finnema E, Van der Wees PJ, Koenders N, Jager-Wittenaar H

“…This study aimed to understand the needs and wishes of community-dwelling older adults regarding interprofessional treatment for (risk of) malnutrition and sarcopenia.Methods: We conducted a grounded theory study. Data collection involved semi-structured interviews and focus groups with community-dwelling older adults who are undergoing treatment or have been treated for (risk of) malnutrition and/or sarcopenia. …”
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Identification of Pivotal ceRNA Networks Associated with Stanford-A Aortic Dissection via Integrated Bioinformatics Analysis by Hu Y, Liu Z, Qin Y, Wu N, Yang T, Cheng X, Wang C, Wang X

“…This study attempts to elucidate the underlying molecular mechanisms of TAAD through lncRNA-associated competitive endogenous RNA (ceRNA) networks.Methods: In this study, aortic vascular of 5 TAAD and 5 control (ischemic heart disease) were subjected to lncRNA and mRNA microarray analysis, and differentially expressed mRNAs (DEGs) and differentially expressed lncRNAs (DELs) were identified. …”
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SIAE-Mediated Loss of Sialic Acid Acetylation Contributes to Ulcerative Colitis by Bo S, Wang X, Qian J, Ma G, Ying Z, Hu D, Hou C, Ma J, Xu L, Yang S

“…This study investigates the role of glycosylation changes, specifically sialic acid de-acetylation, in UC progression.Methods: Tissue samples were obtained from patients with ulcerative colitis (UC) and colorectal cancer at the Second Affiliated Hospital of Soochow University. …”
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A Statistical Framework to Detect and Quantify Operator-Learning Curves in Medical Device Safety Evaluation by Ssemaganda HC, Davis SE, Govindarajulu US, Koola JD, Mao J, Westerman DM, Perkins AM, Speroff T, Ramsay CR, Sedrakyan A, Ohno-Machado L, Matheny ME, Resnic FS

“…The study blinded the analysis team to the data-generation process.Methods: We developed predictive models using generalized additive models and estimated LC parameters using the Levenberg-Marqualdt algorithm. …”
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Serum LEAP2 Levels Across the Spectrum of Metabolic Dysfunction-Associated Fatty Liver Disease: A Potential Noninvasive Biomarker for Severity Stratification by Huang X, Deng Z, Li X, Yan S, Zhong K, Yuan F, Liu L, Deng C, Liu T, Zhao R, Buhe A, Li T, Zhao H

“…Xinyang Huang,1,* Zihao Deng,1,* Xiaozhou Li,2 Songxin Yan,3 Kunjiang Zhong,1 Fengning Yuan,4 Ligang Liu,5 Chaolin Deng,6 Tingting Liu,7 Ruizhao Zhao,1 Amin Buhe,2 Tianxiong Li,2 Hao Zhao2 1Capital Medical University, Beijing, People’s Republic of China; 2Surgery Centre of Diabetes Mellitus, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, People’s Republic of China; 3Department of Clinical Laboratory, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, People’s Republic of China; 4Beijing Stomatological Hospital, Capital Medical University, Beijing, People’s Republic of China; 5Institute of Therapeutic Innovations and Outcomes (ITIO), College of Pharmacy, The Ohio State University, Columbus, OH, USA; 6Department of Hepatobiliary Surgery, Peking University People’s Hospital, Beijing, People’s Republic of China; 7Department of Hepatobiliary Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hao Zhao, Surgery Centre of Diabetes Mellitus, Capital Medical University Affiliated Beijing Shijitan Hospital, Haidian District, 10th Tieyi Road, Beijing, 100038, People’s Republic of China, Tel +86 010 6392 5772, Email 987622272@qq.com Tianxiong Li, Surgery Centre of Diabetes Mellitus, Capital Medical University Affiliated Beijing Shijitan Hospital, Haidian District, 10th Tieyi Road, Beijing, 100038, People’s Republic of China, Tel +86 010 6392 5772, Email doomart@sina.comPurpose: To evaluate circulating liver-expressed antimicrobial peptide 2 (LEAP2) as a potential noninvasive biomarker for the presence of metabolic dysfunction-associated fatty liver disease (MAFLD) and its progression to metabolic dysfunction-associated steatohepatitis (MASH).Patients and Methods: This prospective observational study enrolled obese patients with MAFLD, categorized into simple steatosis (SS) or MASH based on liver histopathology, along with healthy controls (HC). …”
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Vitamin D Alleviates Osteoarthritis Progression by Targeting Cartilage and Subchondral Bone via Myd88-TAK1-ERK Axis Suppression by Gao X, Min Y, Lin R, Liang D, Zhang M, Xiao Q, Lu Y, Zhang F, Xu B, Liu Y

“…Our integrated in vivo/in vitro study demonstrates VitD’s dual chondroprotective and osteogenic actions in OA.Methods: Sprague-Dawley rats (n=24) were divided into three groups: sham operation (Sham), OA model (OA), and OA+VitD treatment, with 8 rats in each group. …”
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RESULTS OF PREVENTIVE WORK IN CHILDREN WITH DIFFERENT FREQUENCY OF ACUTE RESPIRATORY VIRAL INFECTIONS FOLLOWING PREVENTIVE MEASURES IMMEDIATELY AND IN 5 YEAR LONG PERIOD by O.S. Pavlenkova

“…Among the participants, who have frequent SARS cases, the best results were found in the children of 4th group and equalled was 1.83±0.16 points. The children of this group strictly followed the prevention complex we recommended. …”
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Subchronic Chlorpyrifos Exposure Induces Thyroid Follicular Cell Pyroptosis to Exacerbate Thyroid Toxicity by Modulating Nrf2/Keap1/NF-κB Pathway in Male Mice by Gu B, Chen Y, Xu H, Zhan K, Zhu K, Luo H, Huang Y, Zeng H, Zheng W, Tian K, Ruan H

“…This study aimed to investigate the subchronic effects of CPF on thyroid function and elucidate the underlying mechanisms of CPF-induced thyroid toxicity.Methods: The study utilized 4-week-old male C57BL/6J mice as experimental subjects. …”
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Expression of Astragalus membranaceus phenylalanine ammonia-lyase gene in Pichia pastoris by Zhang Jianhui, Wang Shoufeng

“…SDS-PAGE demonstrated that a sharp 78 ku protein which was equal to the predicted value was expressed in the supernatant in contrast to the parent pPIC9K. …”
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Glucagon-Like Peptide 1 Receptor (Glp1r) Deficiency Does Not Appreciably Alter Airway Inflammation or Gut-Lung Microbiome Axis in a Mouse Model of Obese Allergic Airways Disease an... by Kim YJ, Ihrie VM, Shi P, Ihrie MD, Womble JT, Meares AH, Granek JA, Gunsch CK, Ingram JL

“…Thus, we hypothesized that increased GLP-1R signaling following metabolic surgery in obese and allergen-challenged mice leads to gut/lung microbiome alterations, which together contribute to improved features of allergic airways disease.Methods: Male and female Glp1r-deficient (Glp1r−/−) and replete (Glp1r+/+) mice were administered high fat diet (HFD) to induce obesity with simultaneous intranasal challenge with house dust mite (HDM) allergen to model allergic airway disease with appropriate controls. …”
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The Role of Gene of the Tumor Necrosis factor-alfa G-308A Polymorphism in the Development of Congestive Heart Failure in Patients with Ischemic Heart Disease and Obesity by Kravchun P., Kadykova O., Ryndina N., Krapivko S.

“…Materials and research methods. Within the research a complex examination of 222 patients with IHD and obesity has been performed. …”
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Transcriptomic Insights into Adaptive Strategies of Klebsiella pneumoniae Co-Producing KPC-2 and NDM-5 Carbapenemases Under Meropenem Stress by Dai L, Zhang H, Cao Y, Ding J, Tang J, Xiao Z, Chen Z, Ling J, Zou M, Cao X, Lin L, Xu Z, Liu W, Chen D, Yuan P

“…Liting Dai,1,* Huimin Zhang,1,* Yingyi Cao,1,* Jiacheng Ding,1 Jiaxin Tang,2 Zhirou Xiao,2 Zhemei Chen,2 Jiahui Ling,1 Mengxue Zou,1 Xiwu Cao,1 Lijuan Lin,1 Ziheng Xu,1 Wanting Liu,1 Dingqiang Chen,1 Peibo Yuan1 1Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China; 2Department of Clinical Laboratory, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China*These authors contributed equally to this workCorrespondence: Peibo Yuan, Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China, Tel +86 15602335139, Email pbyuanpb@163.com Dingqiang Chen, Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China, Tel +86 15011943079, Email jyksys@126.comBackground: The emergence of Klebsiella pneumoniae strains that co-produce multiple carbapenemases poses significant threats to clinical management; however, the molecular adaptations driving their resilience remain poorly characterized.Methods: This study aimed to investigate the resistance and adaptive mechanisms of the blaKPC-2 and blaNDM-5 co-producing K. pneumoniae KP4 strains. …”
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Long COVID Symptoms and Five Dimensions of HRQoL: A Retrospective Regional Study of Patients Recovered from COVID-19 Infection in Saudi Arabia by Almoliky MA, Alsaif B, Saleh KA, Alkubati SA, Hassan SUN, Algahtani FD, Aldhmadi BKM, Assaggaf HM, Alzain MA

“…Mokhtar Abdo Almoliky,1,2,* Bandar Alsaif,3,* Khalil A Saleh,1,* Sameer A Alkubati,1,4,* Sehar-un-Nisa Hassan,3,* Fahad D Algahtani,3 Badr Khalaf Mubarak Aldhmadi,5 Hamza Mohammad Assaggaf,6 Mohamed Ali Alzain3 1Medical Surgical Nursing Department, College of Nursing; University of Ha’il, Ha’il, Saudi Arabia; 2Nursing Department, Faculty of Medicine and Health Sciences, Taiz University, Taiz, Yemen; 3Department of Public Health, College of Public Health and Health Informatics, University of Ha’il, Ha’il, Kingdom of Saudi Arabia; 4Nursing Department, Faculty of Medicine and Health Sciences, Hodeidah University, Hodeidah, Yemen; 5Department of Health Management, College of Public Health and Health Informatics, University of Ha’il, Ha’il, Saudi Arabia; 6Clinical Laboratory Sciences Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia*These authors contributed equally to this workCorrespondence: Sehar-un-Nisa Hassan, Department of Public Health, College of Public Health and Health Informatics, University of Ha’il, Ha’il, 81451, Kingdom of Saudi Arabia, Email s.nisa@uoh.edu.saBackground/Aim: 10– 20% of people infected with the coronavirus infection have long COVID symptoms, therefore, current research is the first regional assessment in Saudi Arabia to determine the relationship between long-term health complaints of COVID-19 survivors and health-related quality of life (HRQoL).Methods: The study population comprised COVID-19 infection cases registered in the Ha’il region of Saudi Arabia from the beginning of the COVID-19 pandemic until September 2022. …”
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Association of Neutrophil Percentage-to-Albumin Ratio with All-Cause and Cardiovascular Mortality in Maintenance Hemodialysis Patients: A Retrospective Study by Jiang H, Yuan M, Li L, Zhou S, Huang L, Su L, Bai Y

“…This study aims to investigate whether the neutrophil percentage-to-albumin ratio (NPAR), a novel biomarker linked to inflammation, increases the likelihood of both overall and cardiovascular mortality in these patients.Methods: This retrospective cohort study, conducted across multiple centers, utilized data from the China Renal Data System (CRDS) Database collected between 2010 and 2022. …”
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Therapeutic Effects of Fire Needling Acupuncture on Pain Relief and Cartilage Protection in MIA-Induced Knee Osteoarthritis Rats: The Role of Macrophage Polarization in Synovium an... by Wei J, Yang X, Ge X, Zhao L, Liu X, Zhang J, Zhou J, Zhang C, Li W, Li Z, Lyu T, Wang Y, Yuan F, Liu L, Li B

“…This study aimed to evaluate the therapeutic effects of fire needling acupuncture on subchondral bone in KOA.Methods: Sprague-Dawley rats were divided into three groups: control (CON), monosodium iodoacetate (MIA), and fire needling acupuncture (FNA) (n=6 per group). …”
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Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion by Lin Z, Deng C, Wang D, Lyu B, Pan J, Li J, Chen M, Zhang Z, Liang Z, Chen L, Feng S

“…Zhihe Lin,1,* Chuntao Deng,2,* Dan Wang,3,* Bo Lyu,1 Jiaying Pan,4 Jinlian Li,1 Minting Chen,1 Zhaoxia Zhang,5 Zibin Liang,6 Lei Chen,7 Shaoyan Feng1 1Department of Otolaryngology, Head and Neck Surgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 2Department of Radiation Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 3Department of Gastrointestinal Surgery, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 4Department of Otolaryngology, Eye and ENT Hospital, ENT Institute, Fudan University, Zhuhai, Guangdong, People’s Republic of China; 5Office of Organ Transplant Management, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 6Department of Thoracic Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 7Department of Neurosurgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shaoyan Feng, Email fengshaoyan97@163.com; fshaoy@mail.sysu.edu.cn Lei Chen, Email clzzzz@126.comPurpose: This study investigates IL-33 and IL-25 expression in post-radiation otitis media with effusion (POME), along with classic oxidative stress markers (MDA and SOD), to investigate radiation-induced oxidative stress and its association with ILC2-mediated chronic inflammation, providing basis for targeted therapies.Methods: Middle ear effusions (MEE) were collected from 35 irradiated nasopharyngeal carcinoma patients with otitis media with effusion (POME, 43 ears) and 20 non-irradiated conventional chronic otitis media with effusion (CCOME, 20 ears) patients. …”
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Evaluating the Effectiveness of Spousal Testing Among Syphilis-Infected Pregnant Women in the Context of Mother-to-Child Transmission Elimination: A Multicenter Study in Southeaste... by Xue X, Huang X, Liu G, Jiang X, Lin W, Chen Y, Huang C

“…This study assessed spousal testing rates among syphilis-positive pregnant women to guide public health interventions.Methods: This multicenter effectiveness study was conducted in Southeast China, utilizing data from the Chinese HIV/AIDS, Syphilis, and Hepatitis B Prevention Information System between 2018 to 2024. …”
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Lesion Location and Possible Etiology of Acute Unilateral Vestibulopathy by Zhang M, Wang J, Xue S, Liu S, Li K, Zhao T, Feng Y, Sui R, Yang B, Yang X

“…The study aimed to explore the lesion site and possible etiology of AUVP.Methods: This study is a retrospective study. Twenty-three AUVP patients who attended the neurology outpatient clinics of our hospital from January 2020 to March 2022 were included. …”
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