Co-Production of KPC-2 and NDM-5 in a Carbapenem-Resistant Klebsiella Pneumoniae Clinical Isolate: Genetic Insights and Risks by Xia Y, Zhou P, Gao H, Wu X, Zhou Y, Han W, Wan C, Wu Q, Ding J, Yu F

“…Understanding the genetic drivers of resistance and transmission is critical.Methods:  A multidrug-resistant CRKP strain, KP3T58, co-harboring blaKPC-2 and blaNDM-5, was isolated from an ICU patient. …”
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Effect of Remimazolam versus Propofol on Hemodynamics in Elderly Hypertensive Patients Undergoing Gastroenteroscopy: A Multicenter, Randomized Controlled Clinical Trial by Gu Q, Zeng D, Lin D, Zou J, Sun J, Wang H, Chen S, Zhang H, Xu B, Liu W, Shu J

“…Qian Gu,1,2,* Deling Zeng,3,* Dayong Lin,4,* Jiang Zou,5,* Jiaxin Sun,1 Huaiming Wang,1 Shiwei Chen,2 Hongwei Zhang,1 Bo Xu,1 Wusong Liu,6 Jinjun Shu1 1Department of Anesthesiology, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 2Department of Anesthesiology, Xichang People’s Hospital, Xichang, People’s Republic of China; 3Health Management Center, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 4Department of Anesthesiology, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China; 5Department of Anesthesiology, Sichuan Women’s and Children’s Hospital/Women’s and Children’s Hospital, Chengdu Medical College, Chengdu, People’s Republic of China; 6Department of Endoscopy Center, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jinjun Shu, Department of Anesthesiology, Sichuan Cancer Hospital, No. 55, Section 4, South Renmin Road, Chengdu, 610041, People’s Republic of China, Email shujinjun@scszlyy.org.cn Wusong Liu, Department of Endoscopy Center, Sichuan Cancer Hospital, No. 55, Section 4, South Renmin Road, Chengdu, 610041, People’s Republic of China, Email liuwusong@scszlyy.org.cnPurpose: This study aimed to compare the effects of remimazolam and propofol on hemodynamics in elderly hypertensive patients undergoing gastroenteroscopy.Methods: In this multicenter, single-blind, randomized clinical trial, 220 hypertensive patients (65– 75 years) scheduled for gastroenteroscopy were randomly assigned to receive either remimazolam (group R, n=110; 0.3 mg/kg induction followed by 0.2– 1 mg/kg/h maintenance) or propofol (group P, n=110; 1.5 mg/kg induction followed by 2– 6 mg/kg/h maintenance), both combined with 0.1 μg/kg sufentanil. …”
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Analysis of the Regulatory Effects of lncRNAs on Kidney Tissue in Ascites Susceptible Broiler by Kasra Ahmadian, Mohammadreza Nassiry, Karim Hasanpur, Ali Javadmanesh

“…For this reason, the lncRNAs obtained from the RNA-seq data of the kidney tissue were investigated in the occurrence of ascites.Materials and Methods :700 one-day-old chicks from one of the paternal lines of a commercial strain were kept under standard conditions until 21 days old. …”
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Associations Among Obstructive Sleep Apnea, Thyroid Function and Morphology Changes by Xie Y, Zhang H, Cao Z, Zhou Y, Lu C, Yin L, Zhu S, Su Y, Niu X, Ma L, Yuan Y, Zhang Y, Wang Z, Liu H, Ren X, Shi Y

“…Yushan Xie,1,2,* Hongli Zhang,3,* Zine Cao,1,* Yanuo Zhou,1,2 Chendi Lu,1,2 Libo Yin,4 Simin Zhu,1,2 Yonglong Su,1,2 Xiaoxin Niu,1,2 Lina Ma,1,2 Yuqi Yuan,1,2 Yitong Zhang,1,2 Zitong Wang,1,2 Haiqin Liu,1,2 Xiaoyong Ren,1,2 Yewen Shi1,2 1Department of Otorhinolaryngology—Head and Neck Surgery, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Shaanxi Provincial Key Laboratory for Precision Diagnosis and Treatment of Otorhinolaryngology, Xi’an, People’s Republic of China; 3Department of Ultrasound, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of China; 4Otolaryngology Head and Neck Surgery Department, Xi’an Central Hospital, Xi’an, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaoyong Ren, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of China, Email renxiaoyong@sina.vip.com Yewen Shi, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of China, Email shiyewen59@outlook.comPurpose: This study unveils the complex interplay among obstructive sleep apnea (OSA), thyroid function, and morphological changes.Methods: Data from 1,102 patients were collected retrospectively from the Department of Otorhinolaryngology—Head and Neck Surgery of the Second Affiliated Hospital of Xi’an Jiaotong University from 2012 to 2023. …”
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Investigation of Smoking Cessation Status and Its Influencing Factors in Patients with Chronic Obstructive Pulmonary Disease by Fan M, Fang YJ, Chen J, Zhong X, Zhang N, Zeng Z, Xiao D, Qi X, Liang W, Li X, Gao Y, Li S, Su Z

“…This study aimed to investigate the status quo and influencing factors of smoking cessation in patients with SAD or COPD, which is crucial for improving prevention and treatment of chronic airway diseases.Patients and Methods: In this multicenter, prospective cohort investigation, questionnaire survey and one-year follow-up study were conducted in SAD and COPD patients with smoking history. …”
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Antimicrobial efficacy of methylene blue-functionalized graphene oxide-mediated photodynamic therapy against Enterococcus faecalis in root canal disinfection by Rizwan Jouhar, FCPS, Mohamad S. Halim, M.Res.Dent, Sayed A. Quadri, MD, Muhammad A. Ahmed, PhD, Faheem Shah, PhD

“…Abstract: Objective: This study assessed the antimicrobial efficacy of photodynamic therapy (PDT) using functionalized graphene oxide with methylene blue (MB/rGO) to achieve root canal disinfection against Enterococcus faecalis. Methods: In this in vitro study, E. faecalis biofilms were developed on 105 single-rooted teeth after decoronation over 21 days. …”
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Clinical Relevance of Elevated Serum Carcinoembryonic Antigen in Allergic Bronchopulmonary Aspergillosis/Mycosis: A Multicenter Retrospective Study by Ge H, Cai R, Chen X, Liu B, Hu X, Deng S, Li H, Dai L, Tang J, Tang H, Gong X, Wu C, Wang G, Li G, Liu B, Wang J, Tang Y, Li X, Feng J

“…Few studies on ABPA/M cases presenting with elevated serum CEA levels have been reported.Patients and Methods: A cohort of 115 patients diagnosed as ABPA/M were divided into two groups (CEA normal and CEA elevated). …”
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EfficientNetB0-Based End-to-End Diagnostic System for Diabetic Retinopathy Grading and Macular Edema Detection by Long X, Gan F, Fan H, Qin W, Li X, Ma R, Wang L, Hu R, Xie Y, Chen L, Cao J, Shao Y, Liu K, You Z

“…Xin Long,1– 5,* Fan Gan,1– 4,* Huimin Fan,1– 4,* WeiGuo Qin,6 Xiaonan Li,1– 4 Rui Ma,1 Leran Wang,1 Rui Hu,1 Yilin Xie,1 Lei Chen,7 Jian Cao,1– 4 Yinan Shao,1– 4 Kangcheng Liu,1– 4 Zhipeng You1– 4 1Department of Fundus Diseases, The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330000, People’s Republic of China; 2Jiangxi Province Division of National Clinical Research Center for Ocular Diseases, Nanchang, Jiangxi, 330000, People’s Republic of China; 3Jiangxi Clinical Research Center for Ophthalmic Disease, Nanchang, Jiangxi, 330000, People’s Republic of China; 4Jiangxi Research Institute of Ophthalmology and Visual Science, Nanchang, Jiangxi, 330000, People’s Republic of China; 5School of Mathematics and Computer Sciences, Nanchang University, Nanchang, Jiangxi, 330000, People’s Republic of China; 6Department of Cardiothoracic Surgery, The 908th Hospital of Chinese People’s Liberation Army Joint Logistic Support Force, Nanchang, Jiangxi, 330001, People’s Republic of China; 7The Second Clinical School of Medicine, Nanchang University, Nanchang, Jiangxi, 330031, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhipeng You, The Affiliated Eye Hospital, Jiangxi Medical College, Nanchang University, 463 Bayi Road, Nanchang, Jiangxi, 330006, People’s Republic of China, Tel +86 13970032654, Email yzp74@sina.comPurpose: This study aims to develop and validate a deep learning-based automated diagnostic system that utilizes fluorescein angiography (FFA) images for the rapid and accurate diagnosis of diabetic retinopathy (DR) and its complications.Methods: We collected 19,031 FFA images from 2753 patients between June 2017 and March 2024 to construct and evaluate our analytical framework. …”
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Effect of Wuzhuyu Decoction on alcoholic gastric ulcer and its mechanism by GAO Ruiyang, LI Jingyao, LI Pingxiang, GE Keli

“…Objective To investigate the effect of Wuzhuyu Decoction (WZYD) on alcoholic gastric ulcer and its mechanism. Methods A total of 120 female KM mice, aged 8 weeks, were randomly divided into normal control group (group A), model control group (group B), ranitidine group (group C), and low-, middle-, and high-dose WZYD groups (groups D-F), with 20 mice in each group. …”
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Understanding the Needs and Wishes of Older Adults in Interprofessional Treatment for Malnutrition and Sarcopenia: A Grounded Theory Study by Boxum SD, Van Exter SH, Reinders JJ, Drenth H, Van den Berg MG, Tieland M, Geluk-Bleumink A, Spoorenberg SL, Finnema E, Van der Wees PJ, Koenders N, Jager-Wittenaar H

“…This study aimed to understand the needs and wishes of community-dwelling older adults regarding interprofessional treatment for (risk of) malnutrition and sarcopenia.Methods: We conducted a grounded theory study. Data collection involved semi-structured interviews and focus groups with community-dwelling older adults who are undergoing treatment or have been treated for (risk of) malnutrition and/or sarcopenia. …”
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Identification of Pivotal ceRNA Networks Associated with Stanford-A Aortic Dissection via Integrated Bioinformatics Analysis by Hu Y, Liu Z, Qin Y, Wu N, Yang T, Cheng X, Wang C, Wang X

“…This study attempts to elucidate the underlying molecular mechanisms of TAAD through lncRNA-associated competitive endogenous RNA (ceRNA) networks.Methods: In this study, aortic vascular of 5 TAAD and 5 control (ischemic heart disease) were subjected to lncRNA and mRNA microarray analysis, and differentially expressed mRNAs (DEGs) and differentially expressed lncRNAs (DELs) were identified. …”
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SIAE-Mediated Loss of Sialic Acid Acetylation Contributes to Ulcerative Colitis by Bo S, Wang X, Qian J, Ma G, Ying Z, Hu D, Hou C, Ma J, Xu L, Yang S

“…This study investigates the role of glycosylation changes, specifically sialic acid de-acetylation, in UC progression.Methods: Tissue samples were obtained from patients with ulcerative colitis (UC) and colorectal cancer at the Second Affiliated Hospital of Soochow University. …”
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A Statistical Framework to Detect and Quantify Operator-Learning Curves in Medical Device Safety Evaluation by Ssemaganda HC, Davis SE, Govindarajulu US, Koola JD, Mao J, Westerman DM, Perkins AM, Speroff T, Ramsay CR, Sedrakyan A, Ohno-Machado L, Matheny ME, Resnic FS

“…The study blinded the analysis team to the data-generation process.Methods: We developed predictive models using generalized additive models and estimated LC parameters using the Levenberg-Marqualdt algorithm. …”
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Serum LEAP2 Levels Across the Spectrum of Metabolic Dysfunction-Associated Fatty Liver Disease: A Potential Noninvasive Biomarker for Severity Stratification by Huang X, Deng Z, Li X, Yan S, Zhong K, Yuan F, Liu L, Deng C, Liu T, Zhao R, Buhe A, Li T, Zhao H

“…Xinyang Huang,1,* Zihao Deng,1,* Xiaozhou Li,2 Songxin Yan,3 Kunjiang Zhong,1 Fengning Yuan,4 Ligang Liu,5 Chaolin Deng,6 Tingting Liu,7 Ruizhao Zhao,1 Amin Buhe,2 Tianxiong Li,2 Hao Zhao2 1Capital Medical University, Beijing, People’s Republic of China; 2Surgery Centre of Diabetes Mellitus, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, People’s Republic of China; 3Department of Clinical Laboratory, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, People’s Republic of China; 4Beijing Stomatological Hospital, Capital Medical University, Beijing, People’s Republic of China; 5Institute of Therapeutic Innovations and Outcomes (ITIO), College of Pharmacy, The Ohio State University, Columbus, OH, USA; 6Department of Hepatobiliary Surgery, Peking University People’s Hospital, Beijing, People’s Republic of China; 7Department of Hepatobiliary Surgery, Capital Medical University Affiliated Beijing Shijitan Hospital, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hao Zhao, Surgery Centre of Diabetes Mellitus, Capital Medical University Affiliated Beijing Shijitan Hospital, Haidian District, 10th Tieyi Road, Beijing, 100038, People’s Republic of China, Tel +86 010 6392 5772, Email 987622272@qq.com Tianxiong Li, Surgery Centre of Diabetes Mellitus, Capital Medical University Affiliated Beijing Shijitan Hospital, Haidian District, 10th Tieyi Road, Beijing, 100038, People’s Republic of China, Tel +86 010 6392 5772, Email doomart@sina.comPurpose: To evaluate circulating liver-expressed antimicrobial peptide 2 (LEAP2) as a potential noninvasive biomarker for the presence of metabolic dysfunction-associated fatty liver disease (MAFLD) and its progression to metabolic dysfunction-associated steatohepatitis (MASH).Patients and Methods: This prospective observational study enrolled obese patients with MAFLD, categorized into simple steatosis (SS) or MASH based on liver histopathology, along with healthy controls (HC). …”
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Vitamin D Alleviates Osteoarthritis Progression by Targeting Cartilage and Subchondral Bone via Myd88-TAK1-ERK Axis Suppression by Gao X, Min Y, Lin R, Liang D, Zhang M, Xiao Q, Lu Y, Zhang F, Xu B, Liu Y

“…Our integrated in vivo/in vitro study demonstrates VitD’s dual chondroprotective and osteogenic actions in OA.Methods: Sprague-Dawley rats (n=24) were divided into three groups: sham operation (Sham), OA model (OA), and OA+VitD treatment, with 8 rats in each group. …”
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RESULTS OF PREVENTIVE WORK IN CHILDREN WITH DIFFERENT FREQUENCY OF ACUTE RESPIRATORY VIRAL INFECTIONS FOLLOWING PREVENTIVE MEASURES IMMEDIATELY AND IN 5 YEAR LONG PERIOD by O.S. Pavlenkova

“…Purpose of this study is to investigate the Green-Vermilion hygiene index in children who are frequently infected with SARS in 5 years following their undergoing treatment and prevention measures. Materials and methods. 80 children aged 11-12 years who were under the medical observation 5 years ago are examined and divided into 4 groups. …”
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Subchronic Chlorpyrifos Exposure Induces Thyroid Follicular Cell Pyroptosis to Exacerbate Thyroid Toxicity by Modulating Nrf2/Keap1/NF-κB Pathway in Male Mice by Gu B, Chen Y, Xu H, Zhan K, Zhu K, Luo H, Huang Y, Zeng H, Zheng W, Tian K, Ruan H

“…This study aimed to investigate the subchronic effects of CPF on thyroid function and elucidate the underlying mechanisms of CPF-induced thyroid toxicity.Methods: The study utilized 4-week-old male C57BL/6J mice as experimental subjects. …”
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Glucagon-Like Peptide 1 Receptor (Glp1r) Deficiency Does Not Appreciably Alter Airway Inflammation or Gut-Lung Microbiome Axis in a Mouse Model of Obese Allergic Airways Disease an... by Kim YJ, Ihrie VM, Shi P, Ihrie MD, Womble JT, Meares AH, Granek JA, Gunsch CK, Ingram JL

“…Thus, we hypothesized that increased GLP-1R signaling following metabolic surgery in obese and allergen-challenged mice leads to gut/lung microbiome alterations, which together contribute to improved features of allergic airways disease.Methods: Male and female Glp1r-deficient (Glp1r−/−) and replete (Glp1r+/+) mice were administered high fat diet (HFD) to induce obesity with simultaneous intranasal challenge with house dust mite (HDM) allergen to model allergic airway disease with appropriate controls. …”
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Transcriptomic Insights into Adaptive Strategies of Klebsiella pneumoniae Co-Producing KPC-2 and NDM-5 Carbapenemases Under Meropenem Stress by Dai L, Zhang H, Cao Y, Ding J, Tang J, Xiao Z, Chen Z, Ling J, Zou M, Cao X, Lin L, Xu Z, Liu W, Chen D, Yuan P

“…Liting Dai,1,* Huimin Zhang,1,* Yingyi Cao,1,* Jiacheng Ding,1 Jiaxin Tang,2 Zhirou Xiao,2 Zhemei Chen,2 Jiahui Ling,1 Mengxue Zou,1 Xiwu Cao,1 Lijuan Lin,1 Ziheng Xu,1 Wanting Liu,1 Dingqiang Chen,1 Peibo Yuan1 1Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China; 2Department of Clinical Laboratory, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China*These authors contributed equally to this workCorrespondence: Peibo Yuan, Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China, Tel +86 15602335139, Email pbyuanpb@163.com Dingqiang Chen, Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, 510120, People’s Republic of China, Tel +86 15011943079, Email jyksys@126.comBackground: The emergence of Klebsiella pneumoniae strains that co-produce multiple carbapenemases poses significant threats to clinical management; however, the molecular adaptations driving their resilience remain poorly characterized.Methods: This study aimed to investigate the resistance and adaptive mechanisms of the blaKPC-2 and blaNDM-5 co-producing K. pneumoniae KP4 strains. …”
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Long COVID Symptoms and Five Dimensions of HRQoL: A Retrospective Regional Study of Patients Recovered from COVID-19 Infection in Saudi Arabia by Almoliky MA, Alsaif B, Saleh KA, Alkubati SA, Hassan SUN, Algahtani FD, Aldhmadi BKM, Assaggaf HM, Alzain MA

“…Mokhtar Abdo Almoliky,1,2,* Bandar Alsaif,3,* Khalil A Saleh,1,* Sameer A Alkubati,1,4,* Sehar-un-Nisa Hassan,3,* Fahad D Algahtani,3 Badr Khalaf Mubarak Aldhmadi,5 Hamza Mohammad Assaggaf,6 Mohamed Ali Alzain3 1Medical Surgical Nursing Department, College of Nursing; University of Ha’il, Ha’il, Saudi Arabia; 2Nursing Department, Faculty of Medicine and Health Sciences, Taiz University, Taiz, Yemen; 3Department of Public Health, College of Public Health and Health Informatics, University of Ha’il, Ha’il, Kingdom of Saudi Arabia; 4Nursing Department, Faculty of Medicine and Health Sciences, Hodeidah University, Hodeidah, Yemen; 5Department of Health Management, College of Public Health and Health Informatics, University of Ha’il, Ha’il, Saudi Arabia; 6Clinical Laboratory Sciences Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia*These authors contributed equally to this workCorrespondence: Sehar-un-Nisa Hassan, Department of Public Health, College of Public Health and Health Informatics, University of Ha’il, Ha’il, 81451, Kingdom of Saudi Arabia, Email s.nisa@uoh.edu.saBackground/Aim: 10– 20% of people infected with the coronavirus infection have long COVID symptoms, therefore, current research is the first regional assessment in Saudi Arabia to determine the relationship between long-term health complaints of COVID-19 survivors and health-related quality of life (HRQoL).Methods: The study population comprised COVID-19 infection cases registered in the Ha’il region of Saudi Arabia from the beginning of the COVID-19 pandemic until September 2022. …”
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