Semi-automated IT-scATAC-seq profiles cell-specific chromatin accessibility in differentiation and peripheral blood populations by Wei Jin, Jingchun Ma, Li Rong, Shengshuo Huang, Tuo Li, Guoxiang Jin, Zhongjun Zhou

“…We apply IT-scATAC-seq to mouse embryonic stem cells, capturing chromatin remodelling during early differentiation, and to human peripheral blood mononuclear cells, resolving cell-type–specific regulatory programs. …”
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The manufacture of AAV for gene therapy applications using a closed, semi-automated hollow-fiber bioreactor by Adrien Soula, Florian Leseigneur, Amna Anwar, Bilal Ozdoganoglu, Jagan Gurung, Hamza Bhatti, Juline Guenat, Quentin Bazot, Majahar Sayed, Carolina Pinto Ricardo, Lily Li, Katerina Farukshina, Tony Bou Kheir, Hadi Mirmalek-Sani, Gregory Berger, Julie Kerby, Jonathan Appleby, Michael Delahaye

“…In this system, Human Embryonic Kidney 293T cells were successfully expanded and transfected to produce an average crude AAV2 titer of 4.92 × 1014 viral particles and 6.81 × 1013 viral genomes from 1.2 L of harvested cell lysate. …”
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Measurement of the infection and dissemination of bluetongue virus in culicoides biting midges using a semi-quantitative rt-PCR assay and isolation of infectious virus. by Eva Veronesi, Frank Antony, Simon Gubbins, Nick Golding, Alison Blackwell, Peter Pc Mertens, Joe Brownlie, Karin E Darpel, Philip S Mellor, Simon Carpenter

“…At daily intervals 25 Culicoides of each species were removed from incubation, homogenised and BTV quantified in each individual using sqPCR (Cq values) and virus isolation on a KC-C. sonorensis embryonic cell line, followed by antigen enzyme-linked immunosorbent assay (ELISA). …”
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Image Processing Application for Pluripotent Stem Cell Colony Migration Quantification by Timofey Chibyshev, Olga Krasnova, Alina Chabina, Vitaly V. Gursky, Irina Neganova, Konstantin Kozlov

“…Manual extraction of trajectories of cell colonies in experimental image time series is labor intensive and subjective, thus the aim of the work was to develop a computer semi-automated protocol for colony tracking. …”
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Primordial germ cell-mediated chimera technology produces viable pure-line Houbara bustard offspring: potential for repopulating an endangered species. by Ulrich Wernery, Chunhai Liu, Vijay Baskar, Zhor Guerineche, Kamal A Khazanehdari, Shazia Saleem, Jörg Kinne, Renate Wernery, Darren K Griffin, Il-Kuk Chang

“…<h4>Background</h4>The Houbara bustard (Chlamydotis undulata) is a wild seasonal breeding bird populating arid sandy semi-desert habitats in North Africa and the Middle East. …”
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Microphthalmia and Disrupted Retinal Development Due to a LacZ Knock-in/Knock-Out Allele at the Vsx2 Locus by Napoli FR, Li X, Hurtado AA, Levine EM

“…Eye size quantification and immunohistology were used to describe the embryonic and postnatal retinal phenotypes of LacZ homozygous and heterozygous mice. …”
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Replication Past the 𝛾-Radiation-Induced Guanine-Thymine Cross-Link G[8,5-Me]T by Human and Yeast DNA Polymerase 𝜂 by Paromita Raychaudhury, Ashis K. Basu

“…The kind of mutations detected with hPol 𝜂 showed significant similarities with the mutational spectrum of G[8,5-Me]T in human embryonic kidney cells.…”
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