Engineering tripartite gene editing machinery for highly efficient non-viral targeted genome integration by Hangu Nam, Keqiang Xie, Ishita Majumdar, Jiao Wang, Shaobo Yang, Jakob Starzyk, Danna Lee, Richard Shan, Jiahe Li, Hao Wu

“…This ‘tripartite editor with ssDNA optimized genome engineering (TESOGENASE) offers a safer, more efficient alternative to viral vectors for therapeutic gene modification.…”
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GeoMIP-Pattern – a pattern scaling dataset for efficient generation of custom geoengineering scenarios by Rodrigo Muñoz-Sánchez, Bernardo A. Bastien-Olvera, Oscar Calderón, Francisco Estrada Porrúa, Miguel Altamirano

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Evaluation of variability in cell-free DNA extraction efficiency from plasma and urine and spike-in normalization by Fanny Sandberg, Nicholas Kueng, Carlo Rodolfo Largiadèr, Ursula Amstutz

“…We found reproducible extraction efficiencies specific for each method with 84.1% (± 8.17) in plasma, 58.7% (± 11.1) for Zymo, as well as 30.2% (± 13.2) for Qseph based on the 180 bp CEREBIS (Construct to Evaluate the Recovery Efficiency of cfDNA extraction and BISulphite modification) spike-in. …”
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Recycling leather buffing dust into activated carbon for efficient turbidity removal from tannery wastewater by Md Mohsin, Md. Suzaul Karim, Md Ashikur Rahaman Noyon, Mst. Farhana Yeasmin, Md Mojeb Ali, Md. Elias Uddin

“…The maximum removal efficiency attained by the BD derived AC in removing turbidity was 98.39 % at pH 5, 2 g/L dosage and 25 min of contact time. …”
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Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems by Ebrahim Naderlou, Mojtaba Salouti, Bahram Amini, Ali Amini, Asghar Narmani, Ahmad Jalilvand, Reza Shahbazi, Saeid Zabihian

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A spoonful of L‐fucose—an efficient therapy for GFUS‐CDG, a new glycosylation disorder by René G Feichtinger, Andreas Hüllen, Andreas Koller, Dieter Kotzot, Valerian Grote, Erdmann Rapp, Peter Hofbauer, Karin Brugger, Christian Thiel, Johannes A Mayr, Saskia B Wortmann

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Efficient transformation and genome editing in a nondomesticated, biocontrol strain, Bacillus subtilis GLB191 by Yu Zhao, Zhenshuo Wang, Qian Wang, Bing Wang, Xiaoning Bian, Qingchao Zeng, Daowan Lai, Qi Wang, Yan Li

“…The elimination of dam and dcm modifications to mitigate the influence of host restriction-modification systems was integrated to further increase the electroporation efficacy. …”
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Regulatory role of stem-loop structures in Faba bean necrotic yellows virus replication efficiency by Muhammad Amir Qureshi, Muhammad Hassan Butt, Aamir Lal, Thuy T. B. Vo, Nattanong Bupi, Marjia Tabassum, Hyojin Im, Ahlim Lee, Amen Shamim, Sukchan Lee

“…Structural changes affected segment maintenance, emphasizing the need for precise stem-loop architecture for efficient replication. The findings provide novel insights into how FBNYV regulates genome integrity and segment functionality.…”
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A highly efficient heterologous expression platform to facilitate the production of microbial natural products in Streptomyces by Xiuling Wang, Ping Lin, Qiyao Shen, Xueyan Feng, Shouying Xu, Qijun Zhang, Yang Liu, Cailing Ren, Daojing Yong, Qiong Duan, Liujie Huo, Youming Zhang, Gang Li, Jun Fu, Ruijuan Li

“…Results We developed a highly efficient heterologous expression platform, named Micro-HEP (microbial heterologous expression platform), that uses versatile E. coli strains capable of both modification and conjugation transfer of foreign BGCs and optimized chassis Streptomyces strain for expression. …”
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Preparation and Performance of PAN/PS/PMMA Ternary Blend-Modified Fiber Membranes via Centrifugal Spinning for Lithium-Ion Batteries by Shunqi Mei, Feng Luo, Yi Xie, Bin Xu, Quan Zheng

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Research status and prospect of ultra-high speed laser cladding by WEI Chenjun, WANG Yutong, LI Jinghao, ZHAO Zhanyong

“…Ultra-high speed laser cladding technology is the surface modification technology developed in recent years. Due to the characteristics of low dilution rate, small heat-affected zone, and high efficiency, it has been favored by scholars at home and abroad. …”
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Comparative and functional genomic analysis of foreign DNA defense mechanisms in Enterococcus faecium by Alexandra L. Krause, Louise M. Judd, Ryan Wick, Timothy P. Stinear, Andrew H. Buultjens, Ian R. Monk

“…However, the deletion of one or both versions of ardA in E. faecium AUS0233 did not change the transformation efficiency with exogenous DNA, suggesting ArdA in E. faecium AUS0233 is not controlling type I RM. …”
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Advanced Strategies for Mitigating Catalyst Poisoning in Low and High Temperature Proton Exchange Membrane Fuel Cells: Recent Progress and Perspectives by Suyeon Choi, Injoon Jang, Sehyun Lee

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Recent advances in biomimetic nanodelivery systems for cancer Immunotherapy by Jiawei Yang, Xueqi Li, Tongyu Li, Jin Mei, Ying Chen

“…Due to its high targeting, maximum drug delivery efficiency and immune escape, BNDS has become one of the options for tumor immunotherapy in the future. …”
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Surface Engineering‐Induced d‐Band Center Down‐Regulation in High‐Entropy Alloy Nanowires for Enhanced Nanozyme Catalysis by Kunyang Feng, Hanting Wang, Song Zhou, Wei Zhang, Chonghai Gong, Yuxin He, Yusen Wang, Wenchong Dai, Jianbo Li, Zhengwei Zhang, Siqiao Li

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research Paper: Investigating the Improvement of the Hydrophilicity of PP Polymer by Cold Atmospheric Surface Plasma by Mohammad Mahdi Aghamolaei, Fariborz Taghizadeh Kenazeh, Hasan Mehdian, Kamal Hajisharifi

“…Polypropylene (PP), in particular, exhibits surface characteristics that require modification to enhance its hydrophilicity. This research aims to identify an effective method for altering the surface properties of this polymer. …”
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Slow Release Chlorine Dioxide Bactericide Card with Zeolite as Carrier by SUN Peng, TANG Guo-ming, YANG Yi-ning, WU Tong-tong, LU Ya-nan, YANG Bin, LIU Dan-qing, LIU Ying-chun, ZHANG Hongjun

“…Chlorine dioxide disinfectant is widely used in solution form because of its high efficiency, broad-spectrum sterilization ability and environmental friendliness.To address the problem that the application of gaseous chlorine dioxide in water disinfection is relatively small, a high-quality and inexpensive slow-release chlorine dioxide bactericide card was prepared with modified zeolite as the carrier, sodium chlorite as the chlorine dioxide precursor, and cupric nitrate and ferric nitrate as the catalyst.Taking full advantage of the strong permeability and penetration of gaseous chlorine dioxide, it relies on the reaction of water vapor and carbon dioxide in the air with sodium chlorite to achieve stable release of chlorine dioxide.The effects of zeolite modification process, zeolite species, zeolite particle size, the concentration of sodium chlorite and catalyst on chlorine dioxide release rate were further explored.The optimum particle size is 1-2 mm, the optimum calcination temperature is 500℃, and the optimum solution for soaking zeolite is 18% sodium chlorite solution.Adding 0.003 mol/L mixed catalyst of cupric nitrate and ferric nitrate into sodium chlorite soaking solution can improve the release rate of chlorine dioxide.The prepared bactericide card is valid at least for 40 days.…”
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Mesh Stiffness and Dynamic Modeling and Analysis of Modified Straight Bevel Gears by Ding Zhang, Ze-Hua Hu, Wen-Tao Liu, Jin-Yuan Tang, Zhou Sun, Zhao-Yang Tian

“…This paper establishes a dynamic model of the straight bevel gear (SBG) transmission system to accurately and efficiently evaluate the effects of different modification strategies on the vibrational characteristics of SBGs. …”
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Polymer lipid hybrid nanoparticles for phytochemical delivery: challenges, progress, and future prospects by Iqra Rahat, Pooja Yadav, Aditi Singhal, Mohammad Fareed, Jaganathan Raja Purushothaman, Mohammed Aslam, Raju Balaji, Sonali Patil-Shinde, Md. Rizwanullah

“…In addition, the surface modification strategies to improve overall therapeutic efficacy by site-specific delivery of encapsulated phytochemicals are also discussed. …”
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Study on phosphorous-doped cobalt-nitrogen-carbon catalysts for electrocatalytic reduction of carbon dioxide by LI Bingyi, YE Jinze, YE Qingyu, LI Weizhe, LI Feixiang, QIU Ming*

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