Optimizing RNA extraction methods for high-throughput transcriptome sequencing of formalin-fixed paraffin-embedded cardiac tissue specimens. by Nkechi Martina Odogwu, Jin Sung Jang, Sabrina Albertson, Clinton Hagen, Boyd Rasmussen, Oommen Saji, Timothy J Nelson, Wanek Program Preclinical Pipeline

“…Establishing a reliable and reproducible RNA extraction method is critical for generating high-quality transcriptome sequences for molecular assays. Here, the efficiency of four RNA extraction methods: Qiagen AllPrep DNA/RNA method (Method QP); Qiagen AllPrep DNA/RNA method with protocol modification on the ethanol wash step after deparaffinization (Method QE); CELLDATA RNA extraction (Method BP) and CELLDATA RNA extraction with protocol modifications on the lysis step (Method BL) was compared on 23 matching FFPE cardiac tissue specimens (n = 92).In comparing RNA quality metrics across FFPE RNA extract, nucleic acids extracted deploying Method QE and QP produced the highest RNA yield. …”
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Integrating image-generative AI into conceptual design in computer-aided design education: Exploring student perceptions, prompt behaviors, and artifact creativity by Wangda Zhu, Wanli Xing, Eddy Man Kim, Chenglu Li, Yuanzhi Wang, Yoon Yang and Zifeng Liu

“…By analyzing 365 prompts used for completing home tasks, we identified three types of prompt behaviors: generation, modification, and selection, as well as classified three types of workflows: exploration, two-step, and multi-step. …”
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Atmosphere regulation: unraveling effective strategies for creating high-performance iron ore/biochar composite nanomaterials in ball milling processes by Hui Zhang, Zi Cheng, Kai Hu, Boxiong Shen, Honghong Lyu, Jingchun Tang

“…Abstract Ball milling technology has become an important method for material modification due to its high efficiency, environmental protection and economy. …”
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Metal and metal oxide nanomaterials for heavy metal remediation: novel approaches for selective, regenerative, and scalable water treatment by David B. Olawade, David B. Olawade, David B. Olawade, Ojima Z. Wada, Bamise I. Egbewole, Oluwaseun Fapohunda, Abimbola O. Ige, Sunday Oluwadamilola Usman, Olawale Ajisafe

“…Nanomaterial-based approaches have emerged as promising solutions for heavy metal removal, offering enhanced selectivity, efficiency, and sustainability compared to traditional methods. …”
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New horizons in microbial fuel cell technology: applications, challenges, and prospects by Tikam Chand Dakal, Nitesh Singh, Amandeep Kaur, Prabhsangam Kaur Dhillon, Janvi Bhatankar, Ramovatar Meena, Rakesh Kumar Sharma, B. R. Gadi, Bikram Sen Sahu, Asmita Patel, Buddha Singh, Kajal Kumari

“…Recent innovations in nanotechnology have significantly enhanced the performance and efficiency of MFCs by improving electron transfer rates, expanding surface areas, and optimizing the properties of anode and cathode materials. …”
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Dynamics of free radical processes in the oral fluid of patients with chronic recurrent aphthous stomatitis at the first stage of therapy by O. V. Tarasova, A. V. Shumsky, O. N. Pavlova, O. N. Gulenko

“…Significant decrease of the studied indexes of oral liquid testifies to the efficiency of the first stage of therapy of aphthous stomatitis by antiseptic preparations and decrease of intensity of oxidative processes in oral liquid. …”
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Mechanistic understanding of GABA and trehalose in modulating plant response to drought stress by Ambika Choudhary, Eugenie Nepovimova, Vishnu D. Rajput, Tabarak Malik, Monika Choudhary, Nidhi Bhardwaj, Lukas Peter, Sunil Puri, Neetika Kimta

“…It enhances photosynthetic efficiency, regulates reactive oxygen species (ROS), and stabilizes cellular structures. …”
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Physically engineered extracellular vesicles targeted delivering miR-21-5p to promote renoprotection after renal ischemia-reperfusion injury by Di Wu, Wenjie Ma, Liucheng Wang, Chengcheng Long, Silin Chen, Jingyu Liu, Yiguan Qian, Jun Zhao, Changcheng Zhou, Ruipeng Jia

“…Furthermore, this membrane hybrid modification can be tailored and optimized for broader applications in treating other diseases.…”
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Research on Control Method of Electron Accelerator Based on Simulink Simulation by YUE Hongwei1, LI Zhongping2, 3, ZHOU Youwei2, CAO Shuchun2, 3, REN Jieru1, ZHANG Zimin2, 3, , ZHAO Yongtao1

“…Electron accelerators are widely used in material modification, disinfection and sterilization, sewage treatment and other fields. …”

In Situ Halide Vacancy Tuning of Low‐Dimensional Lead Perovskites to Realize Multiple Adjustable Luminescence Performance by Chen Sun, Chang‐Qing Jing, Dong‐Yang Li, Meng‐Han Dong, Ming‐Xue An, Zhong‐Hui Zhang, Cheng‐Yang Yue, Honghan Fei, Xiao‐Wu Lei

“…This is the pioneering discovery in perovskite chemistry for simultaneous modification of multi‐dimensional luminescent performances. …”
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Improved MobileVit deep learning algorithm based on thermal images to identify the water state in cotton by Kaijun Jin, Jihong Zhang, Ningning Liu, Miao Li, Zhanli Ma, Zhenhua Wang, Jinzhu Zhang, Feihu Yin

“…Thermal imaging combined with deep learning algorithms offers an efficient and non-invasive method for monitoring crop water status, facilitating precise irrigation management over large agricultural areas. …”
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Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing by Tianzi Wei, Jiaxuan Li, Xiang Lei, Risheng Lin, Qingyan Wu, Zhenfeng Zhang, Shimin Shuai, Ruilin Tian

“…Summary: Adenosine-to-inosine (A-to-I) RNA editing is a critical post-transcriptional modification that diversifies the transcriptome and influences various cellular processes, yet its regulatory mechanisms remain largely unknown. …”
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M6A-METTL3-dependent nuclear PANC754/PSPC1/H3K4me1 repression complex regulate immune evasive LGALS7 signal to enhance immunotherapy against colorectal cancer by Jianfeng Zhang, Guilian Cao, Feng Li, Siyu Tang, Chenxi Wu, Min Jiang, Jingxin Ye, Shaoqing Ju, Fei Qian, Weifeng Ding

“…Effect is that novel nuclear PANC754/PSPC1/H3K4me1 repression complex down-regulates the level “Don’t eat me” signal LGALS7 to improve the immune efficiency of ICB and induce NK or CTL cell to release perforin and cytokine to kill tumor cells. …”
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Research progress and prospect of underground utilization of coal-based solid waste functional materials by Jixiong ZHANG, Nan ZHOU, Hengfeng LIU, Yachen XIE, Zhihui SUN, Shenyang OUYANG

“…The current research status and development trends comprehensively demonstrate that coal-based solid waste functional materials can not only effectively improve the resource utilization efficiency of coal-based solid waste but also achieve large-scale disposal and value-added utilization of coal-based solid waste.…”
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The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR by Xiaoya Sun, Qinmei Tan, Yue Yang, Jia Wei, Xiaodie Zhou, Shanshan Gao, Fei Yang

“…As an effective and efficient way to regulate biological gene expression, N(6)-methyladenosine(m6A) modification plays an important role in liver injury caused by microcystin-LR(MC-LR) exposure. …”
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Enhanced nitrate reduction by HPMC-stabilized nanoscale zero-valent iron (H-NZVI): Synthesis, characterization and reaction kinetics by Da Ma, Xusheng Li, Huian Zou, Yi Qian, Mengqi Li, Wenchuan Xu

“…Nitrate reduction efficiencies by using iron powder, NZVI, H-NZVI-5, H-NZVI-10, and H-NZVI-20 were examined, and the results showed that a moderate amount of HPMC in modification can help prevent the aggregation of NZVI and achieve high reduction efficiency. …”
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Manganese‐Catalyzed Electrochemical Diazidation of Dehydroalanine Peptides by Xinwei Hu, Chengwei Zheng, Xiaotong Song, Balati Hasimujiang, Mu Chen, Zhixiong Ruan

“…Abstract Dehydroalanine (Dha), a readily introducible non‐canonical amino acid, is widely used for protein post‐translational modification (PTM) and site‐specific labeling of peptides and proteins. …”
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A Modified Porous Sponge with Selective Ability for Oil Removal from Oil-Water Mixtures by Junjie Cheng, Jie Hu, Jiawei Wang, Zefei Huang, Baoqian Ye

“…As oil and chemical spills pose a significant threat to the water environment, the need to develop efficient sorbent materials to remove oil and organic pollutants from water has arisen. …”
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Agricultural Waste–Derived Biochar for Antibiotics Removal in Aqueous Environment: A Comprehensive Outlook by Varalakshmi Varatharajan, Karthik V., Selvakumar Periyasamy

“…Biochar form agricultural waste can efficiently remove antibiotics from aquatic environments and is a renewable and cost-effective adsorbent. …”
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Impact of arm technique and elastic force on vertical jump performance in physical education students: a convergent validation study by Era Deva, Milaim Berisha

“…However, the optimal techniques for maximizing jump height and efficiency for both students and professional soccer players require more effective solutions. …”
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