Understanding experiences of neglected tropical diseases of the skin: a mixed-methods study to inform intervention development in Ethiopia by Michael Marks, David Mabey, Ruth Canter, Elizabeth Allen, Charles Opondo, Jennifer Palmer, Iris Mosweu, Jacob Novignon, Sinéad Langan, Maria Zuurmond, Catherine Pitt, Stephen L Walker, Dorothy Yeboah-Manu, Tara Mtuy, Rachel L Pullan, Richard Phillips, Daniel Okyere, Anthony Ablordey, Samantha Owen, Portia Boakye Okyere, Kidist Bobosha, Katherine Halliday, Steve Walker, Galana Mamo Ayana, Rachel Pullan, Mirgissa Kaba, Tara B Mtuy, Yaw Amoako, Adwoa Asante-Poku, Abigail Agbanyo, Eric Koka, Joseph Timothy, Yohannes Hailemichael, Abebaw Yeshambel Alemu, Teklu Cherkose, Getachew Kebebew, Fikregabrail Aberra Kassa, Tedros Nigusse, Zenebu Begna, Abay Waday, Saba Lambert, Katherine Elizabeth Halliday, Endalamaw Gadisa, Vanessa Yardley, Collins Ahorlu, Anita Achiaa Gyekye, Emmanuel Afreh, Esther Amon, Abraham Kwabena Anang, Ruth Dede Tuwor, Giulia D’Odorico, Kibur Engidawork, Mehari Getaneh, Bernadette Morkporkpor, Emmanuel Narh, Dorcas Opai-Tetteh, Yohannes Michael, Amel Mohammed, Fewzia Shikur Mohammed, Shimelis Doni Nigusse, Maja Niksic, Edmond Okloo, Michael Oppong, Lucy Owusu, Ishaque Siam, Ingrid Sobel, Feleke Tilahum, Joseph Tuffour, Abay Woday, Abebaw Yeshambel, Adelaide Forkuo- Boadu, Derrick Kontoh, Jojo Cobbinah, Kibur Engdawork

“…Transmission discourses shaped different understandings of who was affected by leprosy and CL and expectations of behaviour during and after treatment. Many policy actors felt that existing supply chain interventions, decentralised treatment approaches and community engagement initiatives for leprosy could also benefit CL, but others also warned against increasing care-seeking unless CL treatment could be provided on a scale commensurate with the large burden they perceived.Conclusion Our findings demonstrate significant gaps in the provision of care for skin NTDs within primary healthcare, very different health-seeking patterns for leprosy and CL, and a need to develop new models of care, especially for CL.…”
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Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress by Min Peng, Hui Wang, Shuiping Wen, Zheng Liang, Zheng Huang, Bin Zhang, Tianchong Chen, Qingyun Liu, Qiangyong Li, Yuan Meng, Yuliu Huang, Chunling Yang, Digang Zeng, Manyuan Li, Weilin Zhu, Yongzhen Zhao

“…This study investigated the tissue-specific expression patterns of the heat shock protein genes hsp70, hsp90, and hsp90b in L. vannamei under low-temperature stress, and their correlation with cold tolerance. …”
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Big team science reveals promises and limitations of machine learning efforts to model physiological markers of affective experience by Nicholas A. Coles, Bartosz Perz, Maciej Behnke, Johannes C. Eichstaedt, Soo Hyung Kim, Tu N. Vu, Chirag Raman, Julian Tejada, Van-Thong Huynh, Guangyi Zhang, Tanming Cui, Sharanyak Podder, Rushi Chavda, Shubham Pandey, Arpit Upadhyay, Jorge I. Padilla-Buritica, Carlos J. Barrera Causil, Linying Ji, Felix Dollack, Kiyoshi Kiyokawa, Huakun Liu, Monica Perusquia-Hernandez, Hideaki Uchiyama, Xin Wei, Houwei Cao, Ziqing Yang, Alessia Iancarelli, Kieran McVeigh, Yiyu Wang, Isabel M. Berwian, Jamie C. Chiu, Dan-Mircea Mirea, Erik C. Nook, Henna I. Vartiainen, Claire Whiting, Young Won Cho, Sy-Miin Chow, Zachary F. Fisher, Yanling Li, Xiaoyue Xiong, Yuqi Shen, Enzo Tagliazucchi, Leandro A. Bugnon, Raydonal Ospina, Nicolas M. Bruno, Tomas A. D'Amelio, Federico Zamberlan, Luis R. Mercado Diaz, Javier O. Pinzon-Arenas, Hugo F. Posada-Quintero, Maneesh Bilalpur, Saurabh Hinduja, Fernando Marmolejo-Ramos, Shaun Canavan, Liza Jivnani, Stanisław Saganowski

“…However, such conclusions would be premature because other teams exhibited the opposite pattern. Taken together, results illustrate how big team science can be leveraged to understand the promises and limitations of machine learning methods in affective science and beyond.…”
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Low dietary calcium intake leads to a higher colorectal cancer burden in countries with low social development: findings from the Global Burden of Disease Study 2021 by Junhong Wu, Ziyi Zhang, Jiong Chen, Shiya Yu, Donglin Liu, Junhui Jiang, Tingting Liu, Hu Zhao, Hu Zhao, Yu Wang, Yu Wang

“…Joinpoint regression analysis was conducted to assess temporal patterns in disease burden. Estimated annual percentage changes (EAPCs) were calculated to quantify the rate of change in relevant indicators. …”
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PD-L1 and IFN-γ modulate Non-Small Cell Lung Cancer (NSCLC) cell plasticity associated to immune checkpoint inhibitor (ICI)-mediated hyperprogressive disease (HPD) by Stefania Angelicola, Francesca Giunchi, Francesca Ruzzi, Mariateresa Frascino, Mary Pitzalis, Laura Scalambra, Maria Sofia Semprini, Olga Maria Pittino, Chiara Cappello, Irene Siracusa, Ilaria Candida Chillico, Martina Di Noia, Cristian Turato, Silvia De Siervi, Francesco Lescai, Teresa Ciavattini, Giulia Lopatriello, Luca Bertoli, Hugo De Jonge, Luisa Iamele, Annalisa Altimari, Elisa Gruppioni, Andrea Ardizzoni, Marzia Rossato, Francesco Gelsomino, Pier-Luigi Lollini, Arianna Palladini

“…To assess cell plasticity and aggressiveness, cellular growth patterns were evaluated both in vitro and in vivo through 2D and 3D cell growth assays and patient-derived xenografts establishment. …”
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Comparative analysis of chloroplast genomes on Meliaceae species: insights into the evolution and species identification by Zixuan Nie, Zixuan Nie, Jikai Ma, Jikai Ma, Chengkun Wang, Ming Tang, Ting Jia, Ting Jia, Guoxiang Liao, Guoxiang Liao, Lu Zhang, Lu Zhang

“…Comparative analysis of cp genomes revealed that Meliaceae exhibits relative conservation in terms of cp genome size, inverted-repeat (IR) boundary, genome structure, HVRs and codon patterns. Although differences exist between the Cedreloideae and Melioideae subfamilies, the overall similarity remains notably high. …”
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Dissemination of KPC-2-producing carbapenem-resistant Klebsiella pneumoniae ST792 in Southern China by Cailing Wan, Cailing Wan, Meilan Li, Haojin Gao, Peiyao Zhou, Peiyao Zhou, Bingjie Wang, Junhong Shi, Li Shen, Weihua Han, Xinru Yuan, Jianbo Lv, Jianbo Lv, Yu Huang, Yu Huang, Ying Zhou, Fangyou Yu

“…Conjugation experiments demonstrated successful transfer of two accessory plasmids (p[G5]-2 and p[G5]-3) to Escherichia coli EC600, confirming their mobility and potential role in resistance gene dissemination.ConclusionThis study characterizes the nosocomial dissemination of KPC-2-producing K. pneumoniae ST792 strains, elucidating their antimicrobial resistance patterns and plasmid-mediated transmission mechanisms to inform infection control strategies. …”
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Immune changes in pregnancy: associations with pre-existing conditions and obstetrical complications at the 20th gestational week—a prospective cohort study by David Westergaard, Agnete Troen Lundgaard, Kilian Vomstein, Line Fich, Kathrine Vauvert Römmelmayer Hviid, Pia Egerup, Ann-Marie Hellerung Christiansen, Josefine Reinhardt Nielsen, Johanna Lindman, Peter Christoffer Holm, Tanja Schlaikjær Hartwig, Finn Stener Jørgensen, Anne Zedeler, Astrid Marie Kolte, Henrik Westh, Henrik Løvendahl Jørgensen, Nina la Cour Freiesleben, Karina Banasik, Søren Brunak, Henriette Svarre Nielsen

“…Distinct biomarker patterns were observed for different ethnicities. Within obstetric complications, IL-6 inversely correlated with pre-eclampsia risk, while birth weight to gestational age ratio was linked to markers including VEGF and PlGF. …”
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Dynamics of HBsAb persistence and associated BCR IgG-H CDR3 repertoire features in ultra-high versus extremely low responders to HBV vaccination by Ting-Wen Yang, Ting-Wen Yang, Li-Na Sun, Ting Zhang, Yu-Rong Pan, Jun Li, Yang-Yang Li, Zhi-Yong Han, Wan-Bang Sun, Xin-Sheng Yao, Xiao-Yan He

“…Peripheral blood samples were collected at four distinct phases: pre-vaccination, post-second vaccination, post-third vaccination, and during the follow-up period, to characterize the dynamic changes in the antibody repertoire.ResultsThe longitudinal analysis revealed distinct patterns in antibody response kinetics between the two groups. …”
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Exploring Effects of Age at the Onset of Myopia on Multiple Diseases Using Electronic Health Records by Xiayin Zhang, PhD, Shan Wang, MD, Yu Huang, PhD, Yanjie Xie, PhD, Ishith Seth, MD, Gabriella Bulloch, MD, Chunran Lai, MD, Yijun Hu, PhD, Xianwen Shang, PhD, Mingguang He, PhD, Zhuoting Zhu, PhD, Honghua Yu, PhD

“…The disease-trajectory analysis explored the sequential patterns in childhood-onset and adult-onset groups. …”
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Distribution of alkylamines in surface waters around the Antarctic Peninsula and Weddell Sea by A. Rocchi, A. Rocchi, M. F. Fitzsimons, P. Akenga, A. Sotomayor, E. L. Sà, Q. Güell-Bujons, M. Vila, Y. M. Castillo, M. Dall'Osto, D. Vaqué, C. Wohl, C. Wohl, C. Wohl, R. Simó, E. Berdalet

“…However, knowledge of the sea surface distribution patterns and factors modulating alkylamines remain limited due to their high reactivity and low concentrations, which hamper accurate measurements. …”
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Structure and Function Analyses of the Thioredoxin 2 and Thioredoxin Reductase Gene in Pacific White Shrimp (<i>Litopenaeus vannamei</i>) by Tong Xu, Pei-Hua Zheng, Ke-Er Luan, Xiu-Xia Zhang, Jun-Tao Li, Ze-Long Zhang, Wei-Yan Hou, Li-Min Zhang, Yao-Peng Lu, Jian-An Xian

“…Regarding tissue-specific expression patterns, <i>LvTrx2</i> showed its highest expression levels in hepatopancreas and gill. …”
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How well are implementation strategies and target healthcare professional behaviors reported? A secondary analysis of 204 implementation trials using the TIDieR checklist and AACTT... by Charlene Weight, Billy Vinette, Rachael Laritz, Meagan Mooney, Sonia A. Castiglione, Marc-André Maheu-Cadotte, Nikolas Argiropoulos, Kristin Konnyu, Christine E. Cassidy, Simonne E. Collins, Sonia Semenic, Nicola Straiton, Sandy Middleton, Natalie Taylor, Marie-Pierre Gagnon, Shuang Liang, Laura Crump, Olivia Di Lalla, Talia Meyers, Daniel N. Elakpa, Guillaume Fontaine

“…Descriptive statistics were used to summarize reporting patterns. Data were synthesized narratively and presented in tables, with trends illustrated via a scatterplot. …”
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Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata by Jacob F. Warner, Ryan Besemer, Alicia Schickle, Erin Borbee, Isabella V. Changsut, Koty Sharp, Leslie S. Babonis

“…Further application of these tools will enable functional analyses of embryonic patterning and morphogenesis across Anthozoa and open new frontiers in coral biology research.…”
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Characterization of full-length long noncoding RNAs and identification of virus-responsive lncRNAs in Sogatella furcifera by Yawen Ban, Yawen Ban, Ting Cui, Ting Cui, Lifei Zhao, Lifei Zhao, Xue Li, Xue Li, Qing Bai, Qing Bai, Qingfa Wu, Qingfa Wu

“…Stage-specific lncRNAs were predominantly expressed during embryogenesis (e.g., 93 transcripts between 0 and 72 h post-oviposition) and molting (e.g., 16 in pre-ecdysis), while sex-biased expression patterns emerged after 24 h. …”
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A novel ddPCR™ assay for eDNA detection and quantification of Greater Amberjack Seriola dumerilli and three congeners in US waters: challenges and application to fisheries independ... by P. Joana Dias, Ryan Lehman, Bryan L. Huerta-Beltrán, Ana Wheeler, Crystal L. Hightower, Jessica Heise, Theodore Switzer, Clint Rhode, J. Marcus Drymon, Lynne Stokes, Mark A. Albins, Sean Powers, Nicole Phillips

“…Both eDNA and camera gears detected the presence of S. dumerili and S. rivoliana at both sites and all sampling days, but not S. fasciata and S. zonata. eDNA concentration data was higher for S. dumerili than S. rivoliana at both sites for all sampling days, in line with abundance patterns obtained from camera gears. The highest correlation (r = 0.97) was obtained between the measures of eDNA between gear deployments and ROV. …”
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High spatial resolution crop type and land use land cover classification without labels: A framework using multi-temporal PlanetScope images and variational Bayesian Gaussian mixtu... by Minh Tri Le, Khuong H. Tran, Phuong D. Dao, Hesham El-Askary, Tuyen V. Ha, Taejin Park

“…The results demonstrated the robustness and effectiveness of the proposed framework in mapping crop types and LULC at 3–5 m pixels across various ecosystems, climate zones, and human-managed landscapes. The spatial patterns of PlanetScope-based maps were (1) highly comparable with all the reference datasets at 10–30 m spatial resolution and (2) better than the traditional GMM and K-means clustering methods. …”
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Improved tumor-type informed compared to tumor-informed mutation tracking for ctDNA detection and microscopic residual disease assessment in epithelial ovarian cancer by Mehdi Ben Sassi, Henri Azais, Charles Marcaillou, Sylvain Guibert, Emmanuel Martin, Jérôme Alexandre, Louise Benoit, Aurélien de Reynies, Emilie Laude, Cam Duong, Jacques Medioni, Bruno Borghese, Anne-Sophie Bats, Valerie Taly, Pierre Laurent-Puig

“…Our objective was to compare two ctDNA characterization strategies in EOC for assessing tumor burden during first-line treatment: a tumor-informed approach based on somatic mutations and a tumor-type informed approach utilizing DNA methylation patterns. …”
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A real-life evaluation of SNOT-22 domains in a cohort of CRSwNP patients treated with biologic therapies for 12 months by Giulia Anna Maria Luigia Costanzo, MD, Andrea Giovanni Ledda, MD, Giada Sambugaro, MD, Giuseppe Murdaca, MD, PhD, Cristiano Caruso, MD, PhDs, Silvia Canalis, MD, Paolo Serra, MD, Maria Pina Barca, MD, Stefano Del Giacco, MD, Davide Firinu

“…Conclusion: Our real-life study describes the kinetics over the first year of treatment with mAbs in CRSwNP, showing different patterns in reducing symptoms and improving Health Related Quality of Life (HRQoL). …”
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Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo. by Lisa Deignan, Marco T Pinheiro, Catherine Sutcliffe, Abbie Saunders, Scott G Wilcockson, Leo A H Zeef, Ian J Donaldson, Hilary L Ashe

“…The BMP signaling pathway has a conserved role in dorsal-ventral axis patterning during embryonic development. In Drosophila, graded BMP signaling is transduced by the Mad transcription factor and opposed by the Brinker repressor. …”
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