Optimization of the optimal hormone formula for kiwifruit and analysis of its storage characteristics by Xiaoe He, Wei Qin, Qi He, Yunxiang Liu, Yan Wang, Rencai Wang, Hao Shi, Hao Shi

“…At 80 days of storage, the hardness, good fruit rate, VC content, and TAC of PEHC increased by 68.38%, 28.87%, 32.76 mg·100 g-1, and 20.00%, respectively, compared to the control. Whereas, the difference in SSC of PEHC compared to control was nosignificant. …”
Get full text

Dissecting the tumor microenvironment in primary breast angiosarcoma: insights from single-cell RNA sequencing by Peikai Ding, Shengbin Pei, Yi Zhai, Zheng Qu, Yazhe Yang, Xiaolong Feng, Qiang Liu, Xiangyu Wang, Wenxiang Zhang, Zhongzhao Wang, Xiangyi Kong, Jing Wang, Yi Fang

“…Single-cell RNA sequencing (scRNA-seq) was conducted to capture the transcriptomic profiles of individual cells within the tumor samples. …”
Get full text

Multiomics integration analysis identifies tumor cell-derived MIF as a therapeutic target and potentiates anti-PD-1 therapy in osteosarcoma by Yan Liao, Hao Yao, Weidong Chen, Yutong Zou, Ji Fang, Jiongfeng Zhang, Zehao Guo, Jian Tu, Junkai Chen, Zijun Huo, Lili Wen, Xianbiao Xie

“…Bioinformatics analyses highlighted the differences in immune infiltration patterns and activity between the groups. …”
Get full text

Molecular classification of head and neck squamous cell carcinoma based on hypoxia-related genes and clinical significance of STC2 by ZHU Jianing, WANG Tiantian, ZHANG Rui, SONG Hongquan

“…HNSCC was classified into three subclasses (C1, C2, and C3), and the molecular characteristics and prognostic differences of the subclasses were assessed by comparing the tumor mutation load, functional enrichment analysis, drug sensitivity, and clinical features among the subclasses. …”
Get full text

Machine learning-based diagnostic and prognostic models for breast cancer: a new frontier on the clinical application of natural killer cell-related gene signatures in precision me... by Yutong Fang, Rongji Zheng, Yefeng Xiao, Qunchen Zhang, Junpeng Liu, Jundong Wu

“…With the advent of machine learning (ML) techniques, predictive modeling based on NRGs may offer a new avenue for precision oncology.MethodsWe collected transcriptomic and clinical data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. …”
Get full text

Royal Jelly Enhances the Social Status of Submissive Rats by Restoring Balance to the Disturbed Gut–Brain Communication by Feng Zhu, Jinchun Xu, Tian Wang, Ruili Yang, Biao He, Hui-Li Wang, Yi Xu

“…All behavioral paradigms yielded consistent results, with no notable differences in body weight or anxiety levels. Regarding gut microbiome, vancomycin exposure caused the dysbiosis of the subject rats, which was partially reversed by treatment with royal jelly. …”
Get full text

The functional and clinical significance of nucleoporin NUP153 across human cancers: a systematic study based on multi-omics analysis and bench work validation by Youfu He, Youfu He, Qing Wang, Zhaorui Wang, Menghui Duan, Yu Zhou, Jing Huang, Qiang Wu, Feichang Wu, Chen Li

“…However, the role of NUP153 in cancer, especially its expression patterns, mutations, diagnostic value, and relationship with the tumour immune microenvironment, remains insufficiently explored.MethodsThis study analysed NUP153 expression data from public databases such as TCGA and GTEx. Expression differences between tumour and normal tissues were assessed using Wilcoxon signed-rank tests. …”
Get full text

Identification of prognostic long non-coding RNA and construction of competing endogenous RNA networks in pediatric sepsis by LIU Tiantian, ZHAO Yilin, NING Jingjing, ZHANG Yucai, WANG Chunxia

“…Objective·To screen a long non-coding RNA (lncRNA) signature and construct a competing endogenous RNA (ceRNA) network associated with the prognosis of pediatric sepsis based on the Gene Expression Omnibus (GEO) database, and explore their potential application value in the prognosis assessment of children with sepsis.Methods·Microarray data in GSE4607, GSE26440, GSE26378, and GSE9692 in the GEO database were used to compare the differences in lncRNA profiles between the survival and non-survival groups of children with septic shock. …”
Get full text

Novel Classification of Peroneal Tendon Disease Reveals Cellular and Molecular Phenotypes of the Tenosvnovium by Julia Matthias MD, Michael A. David PhD, Sara E. Buckley DO, Daniel J. Ross MD, Nathaniel Zona BA, Michael A. Hewitt B.A., Joshua Metzl MD, Daniel K. Moon MD, MS, MBA, Courtney Grimsrud MD, Douglas J. Adams PhD, Michael Zuscik PhD, Kenneth Hunt MD

“…Kruskal Wallis with Dunn’s post-hoc was used to detect differences in cellular density between PTD1-4 vs PTD0. …”
Get full text

Transcriptional regulatory network analysis of microglia in multiple sclerosis by CAI Qiangwei, SUN Feng, WU Wenyu, SHAO Fuming, GAO Zhengliang, JIN Shengkai

“…Gene set enrichment analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) was conducted on the DEGs to further explore the biological significance of these differences. Monocle3 was used for pseudotime analysis to study dynamic changes in microglia subpopulations during disease progression. …”
Get full text

Functional loss of rffG and rfbB, encoding dTDP-glucose 4,6-dehydratase, alters colony morphology, cell shape, motility and virulence in Salmonella Typhimurium by Subhashish Chakraborty, Pip Banerjee, Joel P. Joseph, Sanmoy Pathak, Taru Verma, Aagosh Kishor Karhale, Deepti Chandra, Mrinmoy Das, Pritam Saha, Dipankar Nandi

“…There was no significant differences in the growth of the four strains in minimal media at 37°C (nutritional deficiency), in LB at 42°C (high temperature), acidic pH or LB with 3–4% NaCl (high osmolarity; however the ΔrfbBΔrffG strain was hypersensitive to bile and cell wall-targeting antibiotics). …”
Get full text

Integrated multi-omics for potential biomarkers and molecular mechanism of persistent inflammatory refractory rheumatoid arthritis by Ping-Heng Zhang, Ya-Nan Bi, Xiao-Feng Zhao, Kang Chen, En-Sheng Chen, Chang-Hong Xiao

“…Of these, 18 mice per group were used for transcriptomic, proteomic, and metabolomic analyses; 6 for pathological examination and microCT imaging; and 6 for validation experiments. …”
Get full text

Sex and outcomes of patients with microsatellite instability-high and BRAF V600E mutated metastatic colorectal cancer receiving immune checkpoint inhibitors by Michael Hoffmeister, Hermann Brenner, Chiara Cremolini, Sara Lonardi, Filippo Pietrantonio, Julien Taieb, David Tougeron, Robyn L Ward, Marco Vitellaro, Thierry André, Pierre Laurent-Puig, Jitendra Jonnagaddala, Francesca Bergamo, Raghav Sundar, Claire Gallois, Michael J Overman, Jakob Nikolas Kather, Lisa Salvatore, Romain Cohen, Rossana Intini, Priya Jayachandran, Miriam Koopman, Javier Ros, Marwan Fakih, Vincenzo Nasca, Giacomo Mazzoli, Jeanine M L Roodhart, Filippo Ghelardi, Elena Elez, Durgesh Wankhede, Marta Ligero, Joseph Zhao, Koen Zwart, Jeroen Derksen, Nicholas Hawkins, Javier Carmona

“…Finally, we explored two public microarray and RNA-seq datasets from patients with non-metastatic or metastatic MSI-H CRC to gain translational insights on the association between sex, BRAF status and immune contextures/ICI efficacy.Results Although no differences were observed between females and males either overall or in the BRAF wild-type cohort, male sex was associated with inferior progression-free survival (PFS) and overall survival (OS) in the BRAF mutated cohort (in multivariable models, HR for PFS: 1.79, 95% CI: 1.13 to 2.83, p=0.014, and for OS: 2.33, 95% CI: 1.36 to 3.98, p=0.002). …”
Get full text

Spatial patterns and MRI-based radiomic prediction of high peritumoral tertiary lymphoid structure density in hepatocellular carcinoma: a multicenter study by Juan Chen, Xiong Chen, Kai Fu, Lan Zhou, Shichao Long, Mengsi Li, Linhui Zhong, Aerzuguli Abudulimu, Wenguang Liu, Deng Pan, Ganmian Dai, Yigang Pei, Wenzheng Li

“…This study aimed to elucidate biological differences related to pTLS density and develop a radiomic classifier for predicting pTLS density in HCC, offering new insights for clinical diagnosis and treatment.Methods Spatial transcriptomics (n=4) and RNA sequencing data (n=952) were used to identify critical regulators of pTLS density and evaluate their prognostic significance in HCC. …”
Get full text

Identification and role of CmLhcb2.1 in regulating low-light stress resistance in Chinese chestnut (Castanea mollissima) by Yong Yang, Yong Yang, Xuan Wang, Xuan Wang, Jing Liu, Meng Wang, Liyang Yu, Dongsheng Wang, Jingshi Li, Yi Lu, Jingzheng Zhang, Haie Zhang, Haie Zhang

“…CmLhcb2.1 and CmLhcb2.2 were grouped on the same branch with GhLhcb2.3 of upland cotton that involved in chlorophyll synthesis.The chestnut leaves exhibited phenotypic and transcriptomic differences under low and normal light conditions. …”
Get full text

Investigating the eye in Down syndrome as a window to Alzheimer’s disease: the REVEAL protocol – a clinical cross-sectional study by Henrik Zetterberg, Tunde Peto, Imre Lengyel, Kathryn Saunders, Sarah Atkinson, André Strydom, Julie-Anne Little, Elaine Murray, Jamie Mitchell, Aoife Mary Louise Hunter, Lajos Csincsik

“…Between-group differences will be assessed with an independent sample t-test and χ2 tests for normally distributed or binary measures, respectively. …”
Get full text

Attenuated immune surveillance during squamous cell transformation of pancreatic adenosquamous cancer defines new therapeutic opportunity for cancer interception by Jie Chen, Xianlong Chen, Xinyuan Chen, Shanyue Sun, Shuofeng Li, Shuangni Yu

“…Beyond differences in IC density, we observed closer spatial proximity of CD45RO+ and PD-1+CD3+CD8+ T cells to tumor cells within 10, 20 and 30 µm ranges in PASC compared with PDAC, with variations by histological subregion. …”
Get full text

Pre-challenge gut microbial signature predicts RhCMV/SIV vaccine efficacy in rhesus macaques by Hayden N. Brochu, Elise Smith, Sangmi Jeong, Michelle Carlson, Scott G. Hansen, Jennifer Tisoncik-Go, Lynn Law, Louis J. Picker, Michael Gale, Xinxia Peng

“…Despite vaccination group differences, by using newly developed compositional data analysis techniques, we identified a common gut microbial signature predictive of vaccine protection outcome across the three vaccination groups. …”
Get full text

The role of collagen type VI alpha 6 chain as a potential tumor suppressor in breast cancer: an immune regulation perspective by Jian-Di Li, Li-Li Deng, Jia-Yuan Luo, Chao-Hua Mo, Bang-Teng Chi, Wan-Ying Huang, Rong-Quan He, Di-Yuan Qin, Chang Song, Wen Zou, Gang Chen

“…Mouse models, spatial transcriptomic sequencing, and transcriptional regulation analyses were employed to elucidate the intimate relationship between COL6A6 expression and immune cell distribution. …”
Get full text

Screening of reference genes in Callosobruchus maculatus (Coleoptera: Bruchidae) fourth-instar larvae under high-temperature and antibiotic treatments(高温及抗生素处理下四纹豆象四龄幼虫内参基因的筛选)... by 耿加美(GENG Jiamei), 何云川(HE Yunchuan), 陈秋霖(CHEN Qiulin), 高扬(GAO Yang), 周瀛(ZHOU Ying), 祝增荣(ZHU Zengrong)

“…HSP70 expression levels showed significant differences (P＜0.05) between the high-temperature-treated group and the control group after normalization to RpL39_1 or RpL39_2. …”
Get full text