Dietary geniposide supplementation alleviates hepatic lipid deposition and oxidative stress induced by high-fat diet in juvenile tiger puffer, Takifugu rubripes by Haoran Sun, Yonghui Wang, Yinghui Liu, Yi Liu, Junjie Zhang

“…The results showed that no significant differences in growth performance were observed among the three groups (P > 0.05). …”
Get full text

Molecular and immune landscape of melanoma: a risk stratification model for precision oncology by Miao Sun, Junliang Wu

“…Results ssGSEA-based classification revealed significant differences between high and low immune groups in terms of immune infiltration and HLA gene expression. …”
Get full text

Peripheral T lymphocyte immune characteristics dictate response to transarterial chemoembolization in unresectable hepatocellular carcinoma by Lei Peng, Qi Liang, Peng Fei Rong, Shengwang Zhang, Huan Chen, Huaping Liu, Xiaoqian Ma, Wei Wang

“…Conclusion: Our study demonstrated significant differences in the immune characteristics of peripheral T lymphocytes between the Non-Response and Response groups. …”
Get full text

Assessing molecular changes underlying isopropylated phenyl phosphate (IPP)-induced larval sensorimotor response deficits in zebrafish by Sunil Sharma, Alfredo Rojas, Abhishek Gour, Rosemaria Serradimigni, Connor Leong, Abhisheak Sharma, Subham Dasgupta

“…Interestingly, neither of the organs showed differences in 5-mC levels and the brains also did not show substantial transcriptomic effects. …”
Get full text

Dysregulated lipid metabolism in a retinal pigment epithelial cell model and serum of patients with age-related macular degeneration by Ana Álvarez-Barrios, Lydia Álvarez, Pilar Sáenz de Santa María, Montserrat García, Jorge R. Álvarez-Buylla, Rosario Pereiro, Héctor González-Iglesias

“…Four lipids showed significant differences compared to controls: GlcCer(d16:1/18:0) (1.23-fold increase, adj. p value < 0.001), PE(19:1(9Z)/22:2(13Z,16Z)) (0.34-fold decrease, adj. p value < 0.001), PE(15:0/20:3(5Z,8Z,11Z)) (0.66-fold decrease, adj. p value < 0.05), and PC(22:2(13Z,16Z)/13:0) (0.71-fold decrease, adj. p value < 0.05). …”
Get full text

Identification of lipid metabolism-related genes in myocardial infarction: implications for diagnosis and therapy by Qiang Wang, Xian Wu, Bo Yu

“…Materials and methods Endothelial cell transcriptomes from MI patients and controls were downloaded from the Gene Expression Omnibus (GEO) database. …”
Get full text

Calcium-Induced Regulation of <i>Sanghuangporus baumii</i> Growth and the Biosynthesis of Its Triterpenoids by Zengcai Liu, Ying Yu, Shiyuan Wang, Li Zou

“…The observed differences in triterpenoid content were mainly attributed to the activation of the terpenoid backbone biosynthesis pathway and the <i>AACT</i> gene. …”
Get full text

No indications of weight gain associated DNA methylation changes in patients with anorexia nervosa by Luisa Sophie Rajcsanyi, Miriam Kesselmeier, Christopher Schröder, Carolin Schmelting, Triinu Peters, Manuel Föcker, Isabelle Kraft, Jasmin Beygo, Elsa Leitão, Michael Zeschnigk, Johanna Giuranna, Beate Herpertz-Dahlmann, Jochen Seitz, Martina de Zwaan, Wolfgang Herzog, Stefan Ehrlich, Stephan Zipfel, Katrin Giel, Karin Egberts, Roland Burghardt, Bettina Budeus, Johannes Hebebrand, Bernhard Horsthemke, Anke Hinney

Get full text

Decoding disease–specific ageing mechanisms through pathway-level epigenetic clock: insights from multi-cohort validationResearch in context by Pan Li, Jijun Zhu, Shenghan Wang, Haowen Zhuang, Shunjie Zhang, Zhongting Huang, Fuqiang Cai, Zhijian Song, Yuxin Liu, Weixin Liu, Sebastian Freidel, Sijia Wang, Emanuel Schwarz, Junfang Chen

“…Cross-omics validation using transcriptomic data further supported the model's biological relevance (Rho = 0.70, MAE = 7.21). …”
Get full text

Elevated NK and T cell-associated cytokines in plasma are associated with serological response to influenza vaccination by Harry Pickering, Michael A. Carlock, Monica Cappelletti, David W. Gjertson, Ted M. Ross, Ted M. Ross, Ted M. Ross, Elaine F. Reed

“…IntroductionNumerous pre-vaccination factors are known to be associated with differential responses to influenza vaccination, including age, prior infection, vaccination history, immune cell frequencies, and transcriptomic profiles. However, plasma chemokines and cytokines are relatively unexplored. …”
Get full text

Multi-omics mechanical analysis of gut microbiota, carboxylic acids, and cardiac gene expression interaction triggering diabetic cardiomyopathy by Meixin Shi, Bingbing Zhao, Wenjie Cai, Hui Yuan, Xiao Liang, Zhitao Li, Xinyu Liu, Ye Jin, Xi Liu, Can Wei

“…In this study, metagenomics, metabolomics, and transcriptomics were chosen to sequence the changes in gut microbiota, serum metabolite levels, and differentially expressed genes (DEGs) in leptin receptor-deficient db/db mice and analyze the correlation between serum metabolites and gut microbiota or DEGs. …”
Get full text

Molecular characterization and prognostic modeling associated with M2-like tumor-associated macrophages in breast cancer: revealing the immunosuppressive role of DLG3 by Ziqiang Wang, Jing Zhang, Huili Chen, Xinyu Zhang, Kai Zhang, Feiyue Zhang, Yiluo Xie, Hongyu Ma, Linfeng Pan, Qiang Zhang, Min Lu, Hongtao Wang, Chaoqun Lian

“…Consensus clustering analysis identified three molecular subtypes with distinct clinical features, and we explored potential differences in genomic mutations, pathway enrichment, and immune infiltration in patients between subtypes. …”
Get full text

Nuclear hormone receptors control fundamental processes of human fetal neurodevelopment: Basis for endocrine disruption assessment by Katharina Koch, Kevin Schlüppmann, Saskia Hüsken, Louisa Merit Stark, Nils Förster, Stefan Masjosthusmann, Jördis Klose, Arif Dönmez, Ellen Fritsche

“…This study presents novel findings on cellular and molecular hormone actions in human fetal NPCs, highlights major species differences, and illustrates the Neurosphere Assay’s relevance for detecting endocrine MoAs, supporting its application in human-based ED-DNT risk assessment.…”
Get full text

Tracing the evolutionary pathway of SARS-CoV-2 through RNA sequencing analysis by Mostafa Rezapour, Sean V. Murphy, David A. Ornelles, Patrick M. McNutt, Anthony Atala

“…Our first objective was to examine differences in gene expression dynamics using Generalized Linear Models with Quasi-Likelihood F-tests and the Magnitude-Altitude Scoring (GLMQL-MAS) technique, followed by Gene Ontology (GO) and pathway analyses. …”
Get full text

Tripartite Motif-Containing 1 Influences the Prognosis of Cervical Cancer by Ge Zhang, Huan Chen, Yin Tao

“…Methods: TRIM11 expression patterns in CC were investigated through integrated bioinformatics analyses using two independent cohorts: transcriptomic data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) dataset GSE67522. …”
Get full text

Regulatory effects of the bidirectional crosstalk between hepatic gene expressions and gut microbiota on the productive performances and egg quality of induced molting treated laye... by Fuguang Xue, Pengyun Ma, Yunlei Li, Xiaoke Zhang, Xinying Xu, Zhong Ma, Yanyan Sun, Jingwei Yuan, Hui Ma, Jinsong Pi, Huadong Wu, Ling Li, Yuanqi Qu, Jilan Chen

“…Results showed that the egg production rate showed no significant difference between CON and FM groups before FM treatment, while effectively increased after FM treatment. …”
Get full text

Integrative analysis of m6A-SNPs and single-cell RNA sequencing reveals key drivers of endocrine combined with CDK4/6 inhibitor therapy resistance in ER+ breast cancer by Ruijie Ming, Ruijie Ming, Han Zhang, Huan Wu, Fangbiao Zhan, Fangbiao Zhan, Xiaoping Huang, Xiaoping Huang, Huawen Liu, Huawen Liu

“…This study explores the role of N6-methyladenosine (m6A) modifications, influenced by m6A-SNPs, in shaping therapy resistance, utilizing single-cell RNA sequencing to delineate the underlying molecular mechanisms.MethodsWe integrated genome-wide association study data with single-cell transcriptomic profiles from ER+ breast cancer patients, focusing on differences between resistant and sensitive responses to CDK4/6 inhibitors. m6A-SNPs were identified and analyzed for their impact on gene expression and interactions with RNA-binding proteins, with a particular focus on their roles within key cellular pathways.ResultsThe study identified crucial m6A-SNPs associated with therapy resistance. …”
Get full text

Bacteroides uniformis-generated hexadecanedioic acid ameliorates metabolic-associated fatty liver disease by Da-Ya Zhang, Da Li, Shi-Ju Chen, Li-Jun Zhang, Xu-Li Zhu, Fa-Di Chen, Chen Chen, Qi Wang, Yiping Du, Jian-Xin Xiong, Shi-Mei Huang, Xiao-Dong Zhang, Yan-Ting Lv, Fan Zeng, Run-Xiang Chen, Xianfeng Huang, Fengjiao Mao, Shuo Zhou, Qicen Yao, Yuliang Huang, Runyu Chen, Ying Mo, Yunqian Xie, Yue-Hong Jiang, Zhai Chen, Cui-Yi Mo, Jia-Jia Chen, Fei-Hu Bai

“…The mechanisms and microbial-derived metabolites involved in MAFLD were further investigated through multi-omics analyses in vitro and in vivo. Distinct differences were identified in both the microbial community structure and metabolomic profiles between MAFLD patients and healthy controls. …”
Get full text

Distinct Patterns of Smooth Muscle Phenotypic Modulation in Thoracic and Abdominal Aortic Aneurysms by Chien-Jung Lin, Campbell Keating, Robyn Roth, Yasar Caliskan, Mustafa Nazzal, Vernat Exil, Richard DiPaolo, Divya Ratan Verma, Kishore Harjai, Mohamed Zayed, Chieh-Yu Lin, Robert P. Mecham, Ajay K. Jain

“…However, given the summative nature of bulk RNA studies, these findings represent the totality of gene expression without regards to the differences in cellular composition. Single-cell RNA sequencing provides an opportunity to interrogate cell-type-specific transcriptomes. …”
Get full text

Depletion of regulatory T cells enhances the T cell response induced by the neoantigen vaccine with weak immunogenicity by Ruichen Huang, Qiao Zhou, Jiajun Liu, Yang Xia, Yang Jiao, Bi Zhao, Tangtao Feng, Haosu Zhou, Xiuyan Song, Hao Qin, Jun Wang, Lan Cheng, Yunye Ning, Qinying Sun, Yanfang Liu, Xiaoping Su, Yuchao Dong, Wei Zhang

“…We constructed two types of tumor models and investigated differences in therapy efficacy in the four groups (PBS, vaccine, CD25mAb+CTX and combination) at the genetic and protein levels. …”
Get full text