Establishment of an experimental model of canine apocrine gland anal sac adenocarcinoma organoid culture using a three-dimensional culture method by Yuko Nagashima, Haru Yamamoto, Mohamed Elbadawy, Yusuke Ishihara, Issei Tsurukami, Amira Abugomaa, Masahiro Kaneda, Hideyuki Yamawaki, Tatsuya Usui, Kazuaki Sasaki

“…Organoids are three-dimensional cell cultures derived mainly from stem cells and can reproduce tissueʼs epithelial structure, function, and genetics, and thus, of great promise in precision medicine. …”
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Nutritional Composition, Physicochemical Properties, Antioxidant Activity, and Sensory Quality of <i>Matricaria chamomilla</i>-Enriched Wheat Bread by Khawla Kerbab, Ibtissem Sanah, Fairouze Djeghim, Nadjah Belattar, Valentina Santoro, Maria D’Elia, Luca Rastrelli

“…This study evaluates the effects of fortifying bread with different concentrations (3%, 10%, and 30%) of <i>Matricaria chamomilla</i> L. …”
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Visualizing the internalization and biological impact of nanoplastics in live intestinal organoids by Fluorescence Lifetime Imaging Microscopy (FLIM) by Irina A. Okkelman, Hang Zhou, Sergey M. Borisov, Angela C. Debruyne, Austin E. Y. T. Lefebvre, Marcelo Leomil Zoccoler, Linglong Chen, Bert Devriendt, Ruslan I. Dmitriev

“…We report a new method combining adult stem cell-derived small intestinal organoid cultures with live fluorescence lifetime imaging microscopy (FLIM) to study MNP interactions with gut epithelium. …”
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Model-assisted analysis on the response of tomato fruit growth to source-sink ratio regulated by water and nitrogen by Huiping Zhou, Jinliang Chen, Shaozhong Kang

“…The model was then assessed with the data sets from tomatoes grown under different water and N conditions. The results showed that, N deficit and water stress at fruit early /whole growth stage had significant limitation on plant and fruit growth, together with low stem water potential and water consumption. …”
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Design of a Non-Destructive Seed Counting Instrument for Rapeseed Pods Based on Transmission Imaging by Shengyong Xu, Rongsheng Xu, Pan Ma, Zhenhao Huang, Shaodong Wang, Zhe Yang, Qingxi Liao

“…Initially, the U-NET network was used to segment and remove the stem and beak from the pod image; subsequently, adaptive contrast enhancement was applied to adjust the contrast of the G-channel image of the pod to an appropriate range, effectively eliminating the influence of different varieties and maturity levels on the translucency of the pod skin. …”
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Astrocytes contribute to toll-like receptor 2-mediated neurodegeneration and alpha-synuclein pathology in a human midbrain Parkinson’s model by Fiona Weiss, Laura Hughes, Yuhong Fu, Cedric Bardy, Glenda M. Halliday, Nicolas Dzamko

“…Confocal imaging was used to assess lysosomal function and α-syn pathology in the different cell types, as well as DA neuron health and astrocyte activation. …”
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3D cell culture models: how to obtain and characterize the main models by М. М. Abdurakhmanova, A. A. Leonteva, N. S. Vasilieva, E. V. Kuligina, A. A. Nushtaeva

“…An organoid is a more complex 3D model, in which a self-organizing 3D structure is formed from stem cells (SCs) capable of self-renewal and differentiation within the model. …”
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VEGF and IL-6 correlation in POEMS: a potential upcoming marker of active disease and early autologous BMT response. by Luca Laurenti, Idanna Innocenti, Giulia Benintende, Annamaria Tomasso, Francesco Autore, Alberto Fresa, Florenzia Vuono, Silvia Baroni, Claudia Giannotta, Patrizia Chiusolo, Federica Sorà, Simona Sica

“…Methods: We performed a monocentric study, measuring serum levels of VEGF and IL-6 in 8 POEMS pts at different time points of the disease. Results: We observed elevated serum levels of both VEGF and IL-6 in three pts before transplant, while the day after the infusion of autologous stem cells, we observed a steep decrease of both serum markers. …”
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Cloning and expression analysis of sugar transporter protein gene MeSWEET17b in cassava by XUE Jingjing1,2, WEI Zhuowen1, LUO Xiuqin1, AN Feifei1,2*

“…RT-qPCR showed that the relative expressions of MeSWEET17b in leaf and stem had the greatest difference under drought stress. …”
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AI-assisted direct anterior approach versus posterolateral approach in total hip arthroplasty: a retrospective cohort study based on artifact-reduced CT 3D reconstruction by ShanBin Zheng, JiaQing Zhu, ZhiYuan Chen, Xun Cao, TianWei Xia, Chao Zhang, Ji Rong Shen

“…AI-assisted predictions exhibited smaller deviations in DAA for both parameters (FNA: 3.12 ± 5.88° vs. 5.59 ± 8.21°, p = 0.005; AA: 0.93 ± 7.54° vs. −4.34 ± 7.93°, p &lt; 0.001). No significant differences emerged in combined anteversion, acetabular abduction, or femoral stem alignment parameters (all p &gt; 0.05). …”
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In vitro models of microglia: a comparative study by Zoe Woolf, Taylor J. Stevenson, Kevin Lee, Blake Highet, Jena Macapagal Foliaki, Ramona Ratiu, Justin Rustenhoven, Jason Correia, Patrick Schweder, Peter Heppner, Maria Weinert, Natacha Coppieters, Thomas Park, Johanna Montgomery, Amy M. Smith, Michael Dragunow

“…Different in vitro microglia models have been established, including primary human and rodent cells, induced pluripotent stem cell (iPSC)-derived models, and immortalised cell lines. …”
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Macrophages and tumor progression: on the way to macrophage-specific therapy by N. V. Cherdyntseva, I. V. Mitrofanova, M. A. Buldakov, M. N. Stakheeva, M. R. Patysheva, M. V. Zavjalova, J. G. Kzhyshkowska

“…Approaches of simultaneous alteration of cancer stem cells and TAM to abolish chemoresistance and inhibit tumor progression are promising. …”
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Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material by Lakshmi Nidhi Rao, Aditya Shetty, Arun M. Isloor, Sooraj Nayak, Mohana Kumar, Jayaprakasha Shetty K, Muttanna Venkatesh

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Profiling of blood miRNAomes revealed the potential regulatory role of miRNAs in various lameness phenotypes in feedlot cattle by Zhe Pan, Wentao Li, Sonja Bialobzyski, Yanhong Chen, Eoin O’Hara, Hui-zeng Sun, Karen Schwartzkopf-Genswein, Le Luo Guan

“…The predicted functions of identified DE miRNAs among different lameness phenotypes were mainly related to Zinc-finger, muscle cell development, and host inflammatory responses. …”
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Integrin profile of circulating tumour cells in breast cancer patients by E. S. Grigoryeva, L. A. Tashireva, V. V. Alifanov, M. V. Zavyalova, V. M. Perelmuter

“…Numerous studies demonstrate evidence of increased expression of integrins on tumor cells in different types of cancer. Thus, β3 and αV integrins are associated with stem-like features of tumor cells, and β4 integrin as α6β4 heterodimer provides anchorage-independent survival of malignant mammary epithelial cells. …”
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Comparative analysis of anthocyanin content in various tomato samples in connection with breeding by E. A. Dzhos, M. I. Mamedov, O. N. Pyshnaya, N. A. Golubkina, O. V. Verba, A. A. Matyukina

“…Anthocyanin pigments can be synthesised in the stem, leaves, calyx, peel and pulp of tomato fruit. …”
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HIV Modulates Osteoblast Differentiation via Upregulation of RANKL and Vitronectin by Rosa Nicole Freiberger, Cynthia Alicia Marcela López, María Belén Palma, Cintia Cevallos, Franco Agustin Sviercz, Patricio Jarmoluk, Marcela Nilda García, Jorge Quarleri, M. Victoria Delpino

“…We focused on mesenchymal stem cells (MSCs) and osteoblasts, examining their susceptibility to different HIV strains (R5- and X4-tropic) and the subsequent effects on bone tissue homeostasis. …”
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Seahorse nanos3 plays essential roles in germ cell development in the absence of nanos2 by Lingzhen Chen, Lu Tang, Qiang Lin, Yanhong Zhang

“…In particular, they have distinct gonad structures and different patterns of gametogenesis development in both sexes. …”
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A cohort of mRNAs undergo high-stoichiometry NSUN6-mediated site-specific m5C modification by Yuan-Yuan Zhang, Cai-Tao Li, You-Jia Zhou, Hao Li, Jing Li, Qing-Ping Xiong, Wei Zhou, Wenze Huang, Qiangfeng Cliff Zhang, Yangfei Xiang, En-Duo Wang, Beisi Xu, Ru-Juan Liu

“…Here, we biochemically validate that NSUN6, a tRNA structure-dependent methyltransferase, independently catalyzes 5-methylcytidine (m5C) formation with robust activity on mRNA by recognizing the CUCCA motif in a certain stem-loop structure. NSUN6 employs different strategies to recognize tRNA and mRNA substrates. …”
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Integration of Stromal Cells and Hydrogel Below Epithelium Results in Optimal Barrier Properties of Small Intestine Organoid Models by Melis Asal, Maria Thon, Taco Waaijman, Hetty J. Bontkes, Sandra J. van Vliet, Reina E. Mebius, Susan Gibbs

“…Therefore, the aim of this study was to determine whether these stromal cells or ECM were beneficial or detrimental to barrier function when incorporated into an organotypic human small intestine model. …”
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