Construction of a novel inflammatory-related prognostic signature of acute myelocytic leukemia based on conjoint analysis of single-cell and bulk RNA sequencing by Yongfen Huang, Ping Yi, Ping Yi, Yixuan Wang, Lingling Wang, Yongqin Cao, Jingbo Lu, Kun Fang, Kun Fang, Yuexin Cheng, Yuqing Miao, Yuqing Miao

“…This study aims to construct a novel risk model for AML patient through comprehensive analysis of scRNA and bulk RNA data to optimize the precise treatment strategies for patients and improve prognosis.Methods and ResultsscRNA-seq classified cells into nine clusters, including Bcells, erythrocyte, granulocyte-macrophage progenitor (GMP), hematopoietic stem cell progenitors (HSC/Prog), monocyte/macrophagocyte (Mono/Macro), myelocyte, neutrophils, plasma, and T/NK cells. …”
Get full text

The journey of playing: a qualitative evaluation of ‘Maze Out,’ a serious game for eating disorders by Maria Mercedes Guala, Helene Nygaard Nielsen, Kim Bul, David Clinton, Martina Scarpelli, Regina Christiansen, Finn Skårderud, Anette Søgaard Nielsen

“…The game functioned as a transitional object, making the experience of EDs and their challenges more comprehensible and approachable. …”
Get full text

Targeted proteomics for absolute quantification of glucose transporter 1 in mammalian brain cells using liquid chromatography-mass spectrometry by Yash Mehta, Dhavalkumar Patel, Iqra Pervaiz, Ulrich Bickel, Abraham Jacob Al-Ahmad

“…Furthermore, we used such a method to provide absolute GLUT1 quantification in various brain endothelial cells, showing differences in protein expression. Finally, we also used this method to assess changes in GLUT1 protein levels during the differentiation of induced pluripotent stem cells (iPSCs) into astrocyte-like cells (iAstros) and brain microvascular endothelial cell-like cells (iBMECs). …”
Get full text

Daily routines, short-term priorities, and nurses’ role hamper self-management support in a hospital setting: A mixed methods study by Susanne van Hooft, Elke Berger, Cailey van Torenburg, AnneLoes van Staa

“…A paired t-test revealed a significant mean difference of 6.30 95 % CI [5.91–6.69] p ≤ 0.001 between nurses’ perceived self-efficacy (mean = 18.34/24) and their actual performance (mean = 12.03/24) in self-management support. …”
Get full text

Farmers’ knowledge and perception of finger millet blast disease and its control practices in western Kenya by Wilton Mbinda, Agnes Kavoo, Fredah Maina, Margaret Odeph, Cecilia Mweu, Naomi Nzilani, Mathew Ngugi

“…Abstract Background Finger millet blast disease, caused by Pyricularia oryzae, is a serious constrain of finger millet production which is threatening global food security especially to the resource poor smallholder farmers in arid and semi-arid regions. The disease adversely affects finger millet production and consumption due to its wide distribution and destruction in all finger millet growing areas of southern Asia and eastern Africa. …”
Get full text

Phenotypic characterization of Nguni goats in four agro-ecological zones of Limpopo province, South Africa. by Madumetja Cyril Mathapo, Joseph Thinawanga Mugwabana, Thobela Louis Tyasi

“…There was no difference in the qualitative traits of the goats in four agro-ecological zones. …”
Get full text

Comparison of computer-based cognitive stimulation efficacy with and without transcranial direct current stimulation (tDCS) on the quality of life of patients recovered from Covid-... by Alireza Lotfi, Alireza rajaei, mahdi Nayyeri

“…Background: Post-COVID-19 neurological sequelae may have a long-term negative impact on cognitive functions and quality of life in recovered patients. …”
Get full text

Black tea rich in polygonatum sibiricum polysaccharides inhibits osteoporosis in ovariectomized mice through immune regulation and gut microbiota suppression by Shiyu Gao, Yikang Yu, Jialu shu, Biqian Yang, Baiqiao Zhang, Jinhao Xu, Zhenwei Wang

“…This impact is postulated to stem from certain bioactive constituents within CBT that act via immune regulation and gut microbiota modulation.…”
Get full text

Effects of bacterial wilt on community composition and diversity of culturable endophytic fungi in Alpinia galanga by Jiahui Liu, Yuanyuan Wu, Jinru Lin, Mengxia Xie, Likai Chen, Liguo Wang

“…The RA of HDK_J leaves was 78.08% higher than that of stems. All diversity indices for HDK_J were higher than those for HDK_B, with significant differences. …”
Get full text

LABELING EXTRACELLULAR VESICLES FOR HIGHLY SENSITIVE FLOW CYTOMETRY ANALYSES

“…We have optimized a simple flow cytometry method, which, avoiding any pre-enrichment step, allows the identification, classification, enumeration, and separation, by fluorescence activated cell sorting, circulating EVs stemming from different parental cells. This protocol takes advantage of a lipophilic cationic dye (LCD) able to probe EVs, which has been combined with fluorescent phalloidin to exclude damaged elements and different mixes of specific monoclonal antibodies. …”
Get full text

Immunosuppressive role of CD11b+CD33+HLA‐DR− myeloid‐derived suppressor cells‐like blast subpopulation in acute myeloid leukemia by Shin Young Hyun, Eun Jung Na, Ji Eun Jang, Haerim Chung, Soo Jeong Kim, Jin Seok Kim, Jee Hyun Kong, Kwang Yong Shim, Jong In Lee, Yoo Hong Min, June‐Won Cheong

“…Patients with high MDSC‐like blasts at diagnosis showed substantially shorter overall survival and leukemia‐free survival relative to low MDSC‐like blasts patients, with subgroup analysis showing statistically significant differences in patients not receiving allogeneic hematopoietic stem cell transplantation. …”
Get full text

Alimentation et gestion de l’eau sur la colline de Fourvière à Lyon : les aménagements hydrauliques, la ville et ses monuments by Aldo Borlenghi, Éric Leroy

“…The analysis of the remains, and in particular the imposing size of some of the wells, allows us to deduce their public or semi-public function and, for the first time, the existence of hydraulic water drawing devices. …”
Get full text

Production capacity prediction of U-shaped horizontal wells for in-situ pyrolysis of tar-rich coal: Experimental and numerical simulation study by Zhen DONG, Yanpeng CHEN, Mengyuan ZHANG, Xinggang WANG, Lixin JIAO, Shuo FENG, Bo REN, Hao CHEN, Junjie XUE, Yufeng ZHAO, Diefang HUANG

“…CH4 and CO2 increase first and then decrease with temperature, the effect of confining pressure on the two sets of coal is different, and the application of confining pressure is more beneficial to coal gas production of Badaowan Formation coal. ③ Tar-rich coal pyrolysis product yield shows an S-shaped variation pattern of “slow increase - rapid increase - tends to stabilize” with increasing temperature, the Boltsmann function can better fit the law of tar-rich coal pyrolysis product yield. …”
Get full text

Point-of-care N-terminal pro-brain natriuretic peptide testing in patients with heart failure: data from the DREAM study by F. I. Belyalov, N. G. Lozhkina, O. A. Shtegman, R. N. Yagudina, O. V. Kankharey, I. V. Reshina, E. E. Sergeeva, D. F. Belyalov, N. S. Belyalova, Z. Kh. Zhilokov, A. V. Aleynikova, E. A. Medvedeva, A. A. Kharitonov

“…To compare the blood levels of N-terminal pro-brain natriuretic peptide (NTproBNP), determined using a Russian semi-quantitative point-of-care test and quantitative laboratory tests, depending on the presence and severity of heart failure (HF) and left ventricular (LV) function.Material and methods. …”
Get full text

Research of subpopulation CD3+ CD4- CD8- double-negative T lymphocytes in kidney transplant recipients by S. V. Zybleva, S. L. Zyblev

“…The first group included patients with the primary graft function and satisfactory late graft function. The second group included patients with the primary function and late graft dysfunction. …”
Get full text

Pathogenic KCNH2-G53S variant in the PAS domain influences the electrophysiological phenotype in long QT syndrome type 2 by Dasom Mun, Ji-Young Kang, Malgeum Park, Gyeongseo Yoo, Nuri Yun, YouMi Hwang, YouMi Hwang, Boyoung Joung

“…BackgroundLong QT syndrome type 2 (LQT2) is an arrythmia caused by loss-of-function mutations in KCNH2, leading to impaired Kv11.1 channel function.ObjectiveTo better understand LQT2, we examined the electrophysiological differences related to the G53S variant, which is located within the PAS domain of KCNH2, using patient-specific human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs).MethodsWe generated hiPSC-CMs from a patient harboring the KCNH2G53S variant and a healthy control using non-integrative Sendai virus-mediated reprogramming. …”
Get full text

The Feasibility of Using Tartary Buckwheat as a Se-Containing Food Material by Aleksandra Golob, Vekoslava Stibilj, Ivan Kreft, Mateja Germ

“…The aim of this investigation was thus to determine the concentration of Se in different edible parts of Tartary buckwheat treated with different concentrations of Na selenate using different techniques. …”
Get full text

Caregiver and care team perspectives of caregiver psychological distress and well-being during critical care hospitalization: a qualitative study by Amanda C. Blok, Lauren Gauntlett, Mayank Jayaram, Sarah L. Krein

“…Abstract Background Family caregiver psychological distress during an older adult’s critical care hospitalization can compromise their well-being and ability to function in a supportive role for patient recovery. …”
Get full text

Acute effect of proprioceptive exercises on balance, joint position sense and muscle strength among elder and adult by Mansour Sahebozamani, Abdolhamid Daneshjoo, Saeid Bahiraei, Shima Sheikhbahaie, Sanaz Namazi Zadegan, Faeze Firouzi, Elahe Nasrolahzade, Maliheh Soltanabi

“…This study aimed to investigate the immediate impact of proprioceptive exercises on balance, proprioception, and muscle strength in elderly individuals and adults. Methods: In a semi-experimental, 20 adults and 20 elderly individuals (with average ages of 27.20 and 65.73 years) were randomly selected. …”
Get full text

Genome wide identification of AGC kinase genes and their expression in response to heat and cold stresses in barley by Zohreh HAJIBARAT, Abbas SAIDI

“…Identifying overlaps between orthologous clusters can enable us to study the function and evolution of proteins in different species. …”
Get full text