Matrix-free human lung organoids derived from induced pluripotent stem cells to model lung injury by Bettina Budeus, Chiara Kroepel, Lisa Marie Stasch, Diana Klein

“…Abstract Background Organoids, as near-physiological 3D culture systems, offer new opportunities to study the pathogenesis of various organs in mimicking the cellular complexity and functionality of human organs. Method Here we used a quite simple and very practicable method to successfully generate induced pluripotent stem cell (iPSC)-derived human lung organoids (LuOrg) in a matrix-free manner as an alternative to the widely used preclinical mouse models in order to investigate normal lung damage in detail and as close as possible to the patient. …”
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Stem introducer designs and surgical technique play a significant role in achieving the optimal implant-cement interface by Bernard H. van Duren, Kevin Ilo, Sophie Walker, Mark Higgins, Benjamin V. Bloch, Andrew R. Manktelow, Hemant G. Pandit

“…The aim of this study was to assess movement when using different introducer designs. Methods: We compared four stem introducer designs used with two commonly used PTS stems. …”
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Cell-free regenerative medicine: identifying the best source of mesenchymal stem cells for skin therapy in Systemic Sclerosis by Filomena Napolitano, Valentina Giudice, Valentina Giudice, Vittoria D’Esposito, Nella Prevete, Pasqualina Scala, Amato de Paulis, Amato de Paulis, Carmine Selleri, Carmine Selleri, Pietro Formisano, Pietro Formisano, Francesca Wanda Rossi, Francesca Wanda Rossi, Nunzia Montuori, Nunzia Montuori

“…Understanding of the secretion profile of different MSCs is necessary to identify the most efficient and useful source for SSc treatment.MethodsWe analyzed the content of MSC-conditioned media (MSC-CM) obtained from MSCs isolated from adipose tissue (AT), bone marrow (BM), Wharton’s jelly (WJ), and cord blood (CB) by ELISA method, and their effects on the wound healing process by fibroblast proliferation, migration, and ECM deposition assays, to compare regenerative potential of different MSC populations.ResultsWJ-MSC-conditioned medium (CM) and BM-MSC-CM show a greater regenerative profile, compared to CB-MSC-CM and AT-MSC-CM, due to the abundance of growth factors and immunomodulatory cytokines and the effects on fibroblast functions. …”
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Different storage and freezing protocols for extracellular vesicles: a systematic review by Shahin Ahmadian, Negin Jafari, Amin Tamadon, Alireza Ghaffarzadeh, Reza Rahbarghazi, Mahdi Mahdipour

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Investigation of important morphological traits and grain yield of lentil under shading and bio-priming by feresteh darabi, Ali Hatami, Mohammad Javad Zarea, Rahim Naseri

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Deciphering transcriptome patterns in porcine mesenchymal stem cells promoting phenotypic maintenance and differentiation by key driver genes by Nadia Khaveh, Nadia Khaveh, René Buschow, Julia Metzger, Julia Metzger

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Equivalents of the neutrophil-to-lymphocyte ratio of circulating pool of stem and immature hematopoietic cells for assessing liver transplant status by A. N. Shutko, O. A. Gerasimova, N. V. Marchenko, I. I. Tileubergenov

“…Blood samples of 19 recipients with satisfactory graft function were examined by flow cytofluorometry at various time periods after LT using hematopoietic stem cell markers CD133, their CD31 derivatives, and alpha-fetoprotein (AFP), compared with the conventional NLR.Results. …”
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The Optimization of a Protocol for the Directed Differentiation of Induced Pluripotent Stem Cells into Liver Progenitor Cells and the Delivery of Transgenes by Irina Panchuk, Valeriia Kovalskaia, Natalia Balinova, Oxana Ryzhkova, Svetlana Smirnikhina

“…Primary human hepatocytes in 2D cultures rapidly dedifferentiate and lose their function, making their use as a reliable cell model challenging. …”
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Graded Smad2/3 activation is converted directly into levels of target gene expression in embryonic stem cells. by Marcela Guzman-Ayala, Kian Leong Lee, Konstantinos J Mavrakis, Paraskevi Goggolidou, Dominic P Norris, Vasso Episkopou

“…The above system and resource provide tools to study morphogen function in development.…”
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RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF–VEGF complex in extracellular matrix by Changjun Li, Gehua Zhen, Yu Chai, Liang Xie, Janet L. Crane, Emily Farber, Charles R. Farber, Xianghang Luo, Peisong Gao, Xu Cao, Mei Wan

“…Abstract Mesenchymal stem cells (MSCs) participate in the repair/remodelling of many tissues, where MSCs commit to different lineages dependent on the cues in the local microenvironment. …”
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Stem cell transplantation in traumatic spinal cord injury: a systematic review and meta-analysis of animal studies. by Ana Antonic, Emily S Sena, Jennifer S Lees, Taryn E Wills, Peta Skeers, Peter E Batchelor, Malcolm R Macleod, David W Howells

“…Spinal cord injury (SCI) is a devastating condition that causes substantial morbidity and mortality and for which no treatments are available. Stem cells offer some promise in the restoration of neurological function. …”
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Mesenchymal Stem Cell Plasticity: What Role Do Culture Conditions and Substrates Play in Shaping Biomechanical Signatures? by Marina Danalache, Lena Karin Gaa, Charline Burgun, Felix Umrath, Andreas Naros, Dorothea Alexander

“…Cell functionality, driven by remarkable plasticity, is strongly influenced by mechanical forces that regulate mesenchymal stem cell (MSC) fate. …”
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Do STEM education problem-solving tasks trigger learners’ epistemic curiosity? And why we should be astonished by Sebastian Stuppan, Markus Rehm, Tessa J. P. van Schijndel, Markus Wilhelm

“…Conclusions This study presents new evidence on the complex interactions between different epistemic emotions in STEM problem-solving situations, specifically in confrontation tasks. …”
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Microbial Community Characteristics and Underlying Drivers Along the Streams, Tributaries, and Main Stems of the Yangtze River Source Region by Futing Liu, Luyao Kang, Lele Lin, Sisi Yu

“…A comprehensive examination of the riverine microbial community composition and structure is crucial for understanding the functions of aquatic ecosystems. However, previous studies mainly focused on the spatial distribution of microorganisms in the main stems of rivers, ignoring the shifts in microbial communities along the streams, tributaries, and main stems of rivers. …”
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Probability of allogeneic hematopoietic stem cell transplant failure depending on the recipient's killer immunoglobulin-like receptor genotype by U. V. Maslikova, E. G. Khamaganova, M. Yu. Drokov, I. Yu. Urybin, E. D. Mikhaltsova, L. A. Kuzmina, E. N. Parovichnikova

“…Their role is to recognize "self" cells and block signals aimed at destroying their own cells. Knowledge of the functional activity of natural killers urged to studying the impact of mismatches between the inhibitory receptor gene and the ligand on the development of allogeneic hematopoietic stem cell transplant failure.The aim of research was to study the probability of the graft failure development in allogeneic hematopoietic stem cell transplantation depending on the recipient's killer immunoglobulin-like receptor genotype.Material and methods. …”
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CD73 Expression on Mesenchymal Stem Cells Dictates the Reparative Properties via Its Anti-Inflammatory Activity by Kezhe Tan, Hongtao Zhu, Jianfang Zhang, Weili Ouyang, Jianfeng Tang, Youming Zhang, Linlin Qiu, Xueqing Liu, Zhaoping Ding, Xiaoming Deng

“…The results highlight a heterogeneity of the CD73 expression that may be related to its catalytic products on the modulation of the local immune response and thus provide a possible explanation to the inconsistency of the regenerative results when different sources of donor cells were used in stem cell-based therapy.…”
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Clinical application of the tibial extension stem in primary TKA for knee varus deformity combined with tibial bone defect by Fei Yuan, Yankun Li, Xiaogang Shen, Xuepeng Zhu, Li Sun, Youliang Ren, Tao Guo, Bo Li

“…There was no significant difference in HKA Angle between the two groups before and after operation (p > 0.05). …”
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Genome-Wide DNA Methylation Analysis during Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Yangyang Cao, Haoqing Yang, Luyuan Jin, Juan Du, Zhipeng Fan

“…In conclusion, this study revealed the difference of methylated levels during the noninduced and induced osteogenic differentiation of BMSCs and provided useful information for future works to characterize the important function of epigenetic mechanisms on BMSCs’ differentiation.…”
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Investigating combined hypoxia and stemness indices for prognostic transcripts in gastric cancer: Machine learning and network analysis approaches by Sharareh Mahmoudian-Hamedani, Maryam Lotfi-Shahreza, Parvaneh Nikpour

“…Results: Hierarchical clustering identified six clusters among 375 TCGA GC patients, with significant survival differences between cluster 1 (low hypoxia, high stemness) and cluster 4 (high hypoxia, high stemness). …”
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Efficacy and safety of stem cell therapy for acute and subacute ischemic stroke: a systematic review and meta-analysis by Toshiya Osanai, Soichiro Takamiya, Yasuhiro Morii, Katsuhiko Ogasawara, Kiyohiro Houkin, Miki Fujimura

“…Abstract The efficacy of stem cell therapy for ischemic stroke in terms of functional outcomes remains unclear. …”
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