A Benchmark of methods for SARS-CoV-2 whole genome sequencing and development of a more sensitive method by Anthony Bayega, Anthony Bayega, Sarah J. Reiling, Sarah J. Reiling, Ju Ling Liu, Ju Ling Liu, Isabelle Dubuc, Annie Gravel, Louis Flamand, Louis Flamand, Jiannis Ragoussis, Jiannis Ragoussis, Jiannis Ragoussis

“…Here, we aimed to compare efficiencies of SARS-CoV-2 whole genome sequencing approaches using synthetic SARS-CoV-2 genome and six cell culture SARS-CoV-2 variants titrated to represent samples at high, medium, and low viral load. …”
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Noncanonical Poly(A) Polymerase TENT4 Drives Expression of Subgenomic Hepatitis A Virus RNAs in Infected Cells by You Li, Ankit Gupta, Brian N. Papas, David Aponte-Diaz, Jayden M. Harris, Ichiro Misumi, Jason K. Whitmire, Craig E. Cameron, Marcos Morgan, Stanley M. Lemon

“…These findings reveal a novel picornaviral subgenomic RNA species while highlighting mechanistic differences in the manner in which HAV and HBV exploit the host ZCCHC4-TENT4 complex for their replication.…”
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The multidisciplinary approach to the treatment of paediatric chronic pain: a qualitative analysis through focus groups by Aldana Paola Ercolano, Sabrina Galvan, Tessa Louise Marchesan, Alessia Gavioli, Gerardo Papa, Mariarita Todaro, Lidia Berforini D’Aquino, Alessia Zanatta, Andrea Vianello

“…While confirming the efficacy of the holistic approach, the results reveal the need for more efficient coordination between professionals and early involvement of rehabilitation specialists. …”
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Surface functionalization of titanium implants with controlled-release glutathione conjugate for antioxidant and osteogenesis by Huichuan Xu, Kosuke Nozaki, Ziniu Yu, Nobuyuki Kawashima, Takeshi Ueno, Huaze Wu, Hao Wu, Noriyuki Wakabayashi

“…X-ray photoelectron spectroscopy revealed differences in the chemical composition, accompanied by concurrent sulfur and phosphorus peaks, indicating the synthesis and covalent binding of the VPA-GSH conjugates onto the Ti surfaces. …”
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Frenemy: adaptive temperate phage_SAP_1432 supports Staphylococcus aureus survival in changing temperatures by Ting-ting Liu, Peng-cheng Gao, Jie-wen Cui, Wu-bin Wang, Fu-ying Zheng, Xue-rui Li, Yue-feng Chu

“…In this study, we investigated the influence of the heat-resistant temperate phage phage_SAP_1432 on its bacterial host Staphylococcus aureus Q1432 at different temperatures. Thermal host performance curves at a multiplicity of infection of 0.001 showed that the phage killed the bacterium efficiently from 20 to 39°C and from 47 to 50°C. …”
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Changes in the energy metabolism of the gills of Litopenaeus vannamei under carbonate alkalinity stress and recovery conditions by Meng Xiao, Meng Xiao, Yuxiu Nan, Yuxiu Nan, Jitao Li, Yun Wang, Ruijie Zhu, Yafei Duan, Yafei Duan

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Exploring ribosome biogenesis in lung adenocarcinoma to advance prognostic methods and immunotherapy strategies by Zipei Song, Yuheng Wang, Miaolin Zhu, Pengpeng Zhang, Zhihua Li, Xin Geng, Xincen Cao, Jianan Zheng, Jianwei Tang, Liang Chen

“…Cells were scored based on the expression patterns of 331 genes associated with RiboSis across different cell types, and monocle2 was employed to analyze the developmental trajectory of CD4+ T cells. …”
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DA-FIS: A high-speed dynamic adaptive fault injection server framework for reliable FPGA-based embedded systems by Fatimah Alhayan, Gaganjot Kaur, Sultan Alanazi, Mohammed Burhanur Rehman, Wahida Mansouri, Da’ad Albalawneh, Ali Alqazzaz, Hanadi Alkhudhayr

“…This article introduces the dynamic adaptive fault injection server (DA-FIS), a high-speed, scalable, and resource-efficient fault injection framework designed to overcome these limitations. …”
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Solid Surface Vitrification Is Better than Slow Freezing for the Long-Term Preservation of Testicular Fragments from Prepubertal Collared Peccaries (<i>Pecari tajacu</i> Linnaeus,... by Andréia M. Silva, Ana G. Pereira, Gabriel S. C. Bezerra, Yuri G. Matos, Luana G. P. Bezerra, Alexsandra F. Pereira, Moacir F. Oliveira, Pierre Comizzoli, Alexandre R. Silva

“…These collective findings suggest that the combination of SSV with 6 M cryoprotectants is the most efficient for the cryopreservation of testes from prepubertal collared peccaries.…”
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Development of a Conditional Generative Adversarial Network Model for Television Spectrum Radio Environment Mapping by Oluwatobi Emmanuel Dare, Kennedy Okokpujie, Emmanuel Adetiba, Olabode Idowu-Bismark, Abdultaofeek Abayomi, Raymond Jules Kala, Emmanuel Owolabi, Udeme Christopher Ukpong

“…To efficiently use the finite wireless communication resource (radio spectrum), a Radio Environment Map (REM) is needed to monitor, analyse and provide rich awareness of spectrum activities in a radio propagating environment. …”
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Combining inhibition of galectin-3 with and before a therapeutic vaccination is critical for the prostate-tumor-free outcome by Carolina Tiraboschi, Lucas Gentilini, Carla Velazquez, Enrique Corapi, Felipe Martín Jaworski, José Daniel Garcia Garcia, Yorfer Rondón, Anne Chauchereau, Diego José Laderach, Daniel Compagno

“…In the case of immunotherapy, tumors develop robust immune escape mechanisms that abolish any protective response, and to date why prostate cancer is one of the most resistant diseases remains unresolved.Methods By using a combination of clinical data to study the transcriptome of metastasis samples from patients with castration-refractory prostate cancer, and state of the art cellular and molecular biology assays in samples from tumor-bearing mice that have been submitted to surgical resection of the tumor before receiving a vaccination, we answered several essential questions in the field of immunotherapy for prostate cancer. We also used two different methods to inhibit the expression of galectin-3 (Gal-3) in tumor cells: a stable RNA interference method to control the expression of this galectin efficiently only in tumor cells, and low and non-cytotoxic doses of docetaxel to easily transfer our findings to clinical settings.Results Herein, we show for the first time that Gal-3 expressed by prostate tumor cells is the main immune checkpoint responsible for the failure of vaccine-based immunotherapy. …”
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Seasonal, regional, and vertical characteristics of high-carbon-monoxide plumes along with their associated ozone anomalies, as seen by IAGOS between 2002 and 2019 by T. Lebourgeois, T. Lebourgeois, B. Sauvage, P. Wolff, B. Josse, V. Marécal, Y. Bennouna, R. Blot, D. Boulanger, H. Clark, J.-M. Cousin, P. Nedelec, V. Thouret

“…</p> <p>Indian CO anomalies have different characteristics depending on the season, as the wet and dry phases of the monsoon have a strong impact on the transport of the pollutant in this region.…”
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Characteristics of stratified coal reservoirs in Liupanshui coalfield of Guizhou Province and exploration and development direction of coalbed methane by QIU Wenci, SANG Shuxun, GUO Zhijun, HAN Sijie, ZHOU Xiaozhi, ZHOU Peiming, WU Zhangli, SANG Guoyun, ZHANG Binbin, GAO Wei

“…By studying the reservoir characteristics of tectonic coal in Guizhou, adaptable exploration and development technologies are proposed, providing a theoretical basis for the safe and efficient development of tectonic coal reservoirs in the province. …”
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Strategies and Factors to Promote Research in Primary Care in Portugal: A Cross-Sectional Study by Maria Beatriz Morgado, Carolina Penedo, Rubina Correia, Sandra Diniz Amaral, Paulo Jorge Nicola, Margarida Gil Conde

“… Introduction: Research is crucial for building an efficient health system care. This reality is particularly evident in primary care, the cornerstone of healthcare services. …”
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Characterization of a recombinant Sendai virus vector encoding the small ruminant lentivirus gag-P25: antiviral properties in vitro and transgene expression in sheep by Álex Gómez, Idoia Glaria, Irati Moncayola, Irache Echeverría, Javier Arrizabalaga, Ana Rodríguez-Largo, Ignacio de Blas, Delia Lacasta, Estela Pérez, Marta Pérez, Alicia De Diego, Ricardo De-Miguel, Benhur Lee, Lluís Luján, Ramsés Reina

“…Additionally, to characterize the effect of the SRLV restriction in transduced OSF, the SRLV DNA load was quantified at different times post-transduction and post-infection with strain EV1. …”
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Exploring the Realization of Creative Dimensions within the Metaverse: The Case of Tabriz Metropolis by Saied Jaffari namvar, Hossein Nazmfar, Ibrahim Taghav

“…This study showed different results in different models. The results indicate that regions 5 and 8 rank the most suitable regions, while regions 9 and 6 are identified as having the least favorable conditions in terms of the evaluated indicators. …”
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Design of an improved model using federated learning and LSTM autoencoders for secure and transparent blockchain network transactions by R. Vijay Anand, G. Magesh, I. Alagiri, Madala Guru Brahmam, Balamurugan Balusamy, Chithirai Pon Selvan, Haya Mesfer Alshahrani, Masresha Getahun, Ben Othman Soufiene

“…Our proposed framework is effective according to the interim outcomes where the aggregation of local models occurred without data leakage, detected anomalies very efficiently, managed models very transparently, with privacy of data at a very high level, and easily detected fraudulent transactions. …”
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Performance of Large Language Models ChatGPT and Gemini on Workplace Management Questions in Radiology by Patricia Leutz-Schmidt, Viktoria Palm, René Michael Mathy, Martin Grözinger, Hans-Ulrich Kauczor, Hyungseok Jang, Sam Sedaghat

“…Our study revealed that LLMs have the potential to improve workplace management in radiology by assisting with various tasks, making these processes more efficient without requiring specialized management skills.…”
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Point-of-care ultrasound clinical workflow pilot: design and adoption of a simplified automated workflow within EHR for all clinical settings by Viveta Lobo MD, Ashley Erin Hall MD, Zahra-Ghazi Askar MD, Luyao Shen MD

“…Over the first month, this solution was utilized 750 times by 67 different clinicians with 17 individual PoCUS orders and 4 combination PoCUS orders. …”
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Validation of a questionnaire to assess complexity of palliative care needs in primary care in Malaga, Spain: a study protocol by Jose Miguel Morales-Asencio, Rafael Gomez-Garcia, Pilar Barnestein-Fonseca, Francisca Leiva-Fernandez, Auxiliadora Fernández-López, Maria Luisa Martín-Roselló, Virginia Patricia Aguiar-Leiva

“…This protocol was conducted without patient or public participation.Ethics and dissemination This study evaluates a novel, co-designed tool to diagnose PC complexity to inform practice recommendations for a more efficient allocation of resources that may be included in future clinical practice guidelines. …”
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