Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha by Senqin Hou, Shibin Yang, Wenqin Bai

“…Furthermore, using this developed CRISPR-Cpf1 system, triple genes were precisely integrated into the genome by one-step, enabling lycopene production in O. polymorpha. …”
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Amplitude-frequency characteristics of the seat vibration protection system with a three-segment static force characteristic with a section of quasi-zero stiffness by Korytov M.S., Sherbakov V.S., Kashapova I.E.

“…The design scheme has been developed, key parameters have been selected, and their study on vibration protection parameters has been carried out. …”
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Training of Oral and Maxillofacial Surgery Residents in Virtual Surgical Planning: A Feasibility Study Comparing Open-Source Freeware and Commercially Available Software for Mandib... by Bert Rombaut, Matthias Ureel, Benjamin Van der Smissen, Nicolas Dhooghe, Renaat Coopman

“…The duration (minutes) and the amount of mouse clicks (MCs) of every step in the workflow were recorded. Afterwards, the experience with VSP was investigated with the System Usability Scale (SUS) and a self-developed questionnaire. …”
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Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation. by Claes-Göran Reibring, Maha El Shahawy, Kristina Hallberg, Marie Kannius-Janson, Jeanette Nilsson, Seppo Parkkila, William S Sly, Abdul Waheed, Anders Linde, Amel Gritli-Linde

“…We showed that expression of CAs in developing teeth is not confined to cells involved in biomineralization, pointing at their participation in other biological events. …”
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Chaos control and sensitivity analysis of climate change under green gases and carbon omission utilizing caputo fractional operator by Aqeel Ahmad, Muhammad Suleman Khan, Dilber Uzun Ozsahin, Hijaz Ahmad, Arshad Munir, Taha Radwan

“…For the said purpose, numerical simulations under a two-step Lagrange polynomial technique are employed. …”
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Acute rheumatic fever and rheumatic heart disease: what's new? by D. Yu. Andriyashkina, N. A. Shostak, A. V. Aksenova, D. V. Abeldyaev

“…Rheumatic heart disease (RHD) is a preventable heart disease that remains endemic in developing countries. More than 30 million people in the world suffer from RHD, of which approximately 300,000 die every year, despite the fact that this disease is preventable and treatable. …”
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Optimal Design of a Sensor Network for Guided Wave-Based Structural Health Monitoring Using Acoustically Coupled Optical Fibers by Rohan Soman, Jee Myung Kim, Alex Boyer, Kara Peters

“…As a result, a sensor network with multiple sensing locations and a few actuators is feasible and cost effective. This paper develops a two-step methodology for the optimization of an actuator–sensor network harnessing the acoustic coupling ability of FBG sensors. …”
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Enhancing consistency of AHP-based expert judgements: A new approach and its implementation in an interactive tool by Shimon Frish, Irit Talmor, Omer Hadar, Maxim Shoshany, Aviad Shapira

“…The proposed tool has broad potential applications in various complex decision-making scenarios. In future developments, the tool could also be adapted to incorporate clustering techniques for managing complex criteria sets, or extended to support group decision making by assessing consensus levels and recommending minimal adjustments. • AHP-based pairwise comparisons of n criteria by experts are recorded in an n × n reiprocal preference matrix whose eigenvalu λmax is used to compute the consistency ratio (CR) of the resulting priority vector. • An iterative greedy algorithm developed to enhance the consistency of the solution in case CR>10% enforces minimal intervention by limiting the adjustment to single-step modifications on unaltered pairwise comparisons only. • The algorithm is implemented in an interactive, user-friendly software tool, ensuring transparency and expert engagement through real-time, traceable consistency refinement.…”
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Experience of Acceptance and Commitment Therapy for those with mild traumatic brain injury (ACTion mTBI): A qualitative descriptive study. by Josh W Faulkner, Jason Chua, Amabelle Voice-Powell, Deborah L Snell, Maree Roche, John Moffat, Suzanne Barker-Collo, Alice Theadom

“…Consequently, we developed ACTion mTBI, a 5-session ACT-informed intervention protocol. …”
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PLASMOpred: A Machine Learning-Based Web Application for Predicting Antimalarial Small Molecules Targeting the Apical Membrane Antigen 1–Rhoptry Neck Protein 2 Invasion Complex by Eugene Lamptey, Jessica Oparebea, Gabriel Anyaele, Belinda Ofosu, George Hanson, Patrick O. Sakyi, Odame Agyapong, Dominic S. Y. Amuzu, Whelton A. Miller, Samuel K. Kwofie, Henrietta Esi Mensah-Brown

“…Red cell invasion is a crucial step in the parasite’s life cycle, where the parasite invade human erythrocytes to sustain infection and ensure survival. …”
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Computer-vision based automatic rider helmet violation detection and vehicle identification in Indian smart city scenarios using NVIDIA TAO toolkit and YOLOv8 by Uttam U. Deshpande, Goh Kah Ong Michael, Sufola Das Chagas Silva Araujo, Vaidehi Deshpande, Rudragoud Patil, Ramchandra Alias Ameet Chate, Varun R. Tandur, Supreet S. Goudar, Shreya Ingale, Vaishnavi Charantimath

“…Experimental analysis shows that our proposed two-step model achieved a promising helmet detection accuracy of 98.56% and a 97.6% number plate detection accuracy of persons not wearing helmets. …”
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Normative values to assess functional fitness in older adults in a region of Chile by Marco Cossio-Bolaños, Rubén Vidal-Espinoza, Luis F. Castelli Correia de Campos, Jose Sulla-Torres, Camilo Urra-Albornoz, Fernando Alvear-Vasquez, Jose Fuentes-Lopez, Marcella Silva Ramos de Lazari, Rossana Gomez-Campos

“…In the 8-ft up-and-go test, discrepancies ranged from ∼1.1 to 4.4 s. In the aerobic 2-min step test, discrepancies ranged in both sexes from ∼21 to 41 repetitions. …”
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A randomized pilot study comparing the role of PEEP, O2 flow, and high-flow air for weaning of ventilatory support in very low birth weight infants by Chang-Yo Yang, Mei-Chin Yang, Shih-Ming Chu, Ming-Chou Chiang, Reyin Lien

“…Background: There is a lack of evidence to guide step-wise weaning of positive pressure respiratory support for premature infants. …”
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Characterizing the Temporally Dynamic Nature of Relative Growth Rates: A Kinetic Analysis on Nitrogen-, Phosphorus-, and Potassium-Limited Growth by Andrew Sharkey, Asher Altman, Yuming Sun, Thomas K. S. Igou, Yongsheng Chen

“…Developing precision models to describe agricultural growth is a necessary step to promote sustainable agriculture and increase resource circulation. …”
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A Model of Human Resource Management System Suitable for the New Generation Z in Executive Organizations with Thematic Analysis Approach by Moein Pourahmadi, Saeed Jafarinia, Yusuf Vakili, Akbar Hasanpour

“…Objective The continuity of a society is ensured by the process of socialization that transfers the social traditions of previous generations to new generations; that is, new generations never exactly repeat the social life of their predecessors step by step, but are always accompanied by criticism of some aspects of tradition or innovation. …”
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Theory of coal wall spalling and cooperative control by “support-destressing” in 10 m ultra-large mining height working face by Jiangbin LIU, Zhizeng HUANG, Qianjin LIU, Zhen ZHANG, Cundi HAN, Xingyu LIN, Rongshan MA, Zhen ZHAO

“…The failure of coal wall preferentially develops in the direction of the maximum horizontal stress unloading degree and the minimum lateral pressure coefficient. …”
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Classification and identification of yeast strain A82-2 and its inhibitory effect on Rhizopus stolonifer by ZHOU Xiaohong, YAN Dong, LU Zhaoxin, LU Fengxia, SHEN Chang, BIE Xiaomei

“…Regarding to classification and identification of strain A82-2, we used general classification methods such as observation of the yeasts morphology, and characterization of physiology and biochemistry, supplemented with molecular biology methods to analyze 26S rDNA sequence.First of all, using four different antifungal experiments step-by-step demonstrated the obvious inhibitory effect of yeast A82-2 on R. stolonifer. …”
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Mixed Reality Ultrasound-Guided Mini-ECIRS with Apple Vision Pro™ - First Case Report by Roberto Montoro Neto, Fabio C. Vicentini, Ricardo T.S. Ugino, Alexandre Danilovic, Giovanni S. Marchini, Fabio C.M. Torricelli, Carlos A. Batagello, Anderson B. Pellanda, Alexandre Silva, William C. Nahas, Eduardo Mazzucchi

“…Objective: To demonstrate the step-by-step technique of the Mini-Endoscopic Combined Intra-Renal Surgery (Mini-ECIRS) procedure guided by ultrasound and using mixed reality technology with the Apple Vision Pro (multiscreen and 3D reconstruction). …”
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Simulação no contexto de problemas mal estruturados by Antônio Sérgio de Souza, Marcos Augusto da Silveira, Mônica Santos de Pontes, Olympio de Andrade Jr, Wagner Barbosa

“…It is also dicussed the necessity of developing the conceptual modeling of the real system, not only as a separated step in the simulation process, but in a progressive and dynamic way, during the project's life cycle as an interface between the real system and simulation.…”
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Improving food packaging safety through cold plasma-treated edible films with Carum copticum extracts by Fatemeh Ghadiminejad, Akbar Esmaeili, Manzarbanou Asnaashariisfahani

“…Abstract This research addresses the growing need for sustainable food packaging solutions by developing composite edible films that integrate Carum copticum extract into a matrix of chitosan, polyethylene, fish gelatin, gum arabic, and corn starch. …”
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