Development and Validation of One-Step Reverse Transcription-Droplet Digital PCR for Plum Pox Virus Detection and Quantification from Plant Purified RNA and Crude Extract by Giorgia Bertinelli, Lorenza Tizzani, Marta Luigi, Simona Monticelli, Vincenza Ilardi

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A novel approach for detecting Salmonella enterica strains frequently attributed to human illness—development and validation of the highly pathogenic Salmonella (HPS) multiplex PCR assay by Dayna M. Harhay, Kerry D. Brader, Tatum S. Katz, Gregory P. Harhay, James L. Bono, Joseph M. Bosilevac, Tommy L. Wheeler

“…The data presented lay the groundwork for development of rapid commercial assays for the detection of SoC.…”
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Sentiment Analysis Using a Large Language Model–Based Approach to Detect Opioids Mixed With Other Substances Via Social Media: Method Development and Validation by Muhammad Ahmad, Ildar Batyrshin, Grigori Sidorov

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Development of an updated loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Ralstonia syzygii subsp. celebesensis (Rsc), the causative agent of banana blood disease by Kaennuea Thipsukon, Thanyasiriwat Thanwanit, Rattanapolsan Ladawan, Ponpang-Nga Pancheewan, Sangdee Aphidech, Kawicha Praphat

“…This study developed and assessed a loopmediated isothermal amplification (LAMP), which detected as few as 10 cells per reaction, significantly surpassing conventional PCR, which required a minimum of 10³ cells per reaction. …”
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Development and implementation of a TaqMan triplex real-time PCR assay for concurrent detection of pseudorabies virus, porcine teschovirus 1, and Streptococcus suis 2 by Ranran Lai, Ranran Lai, Chen Yang, Chen Yang, Lili Wu, Lili Wu, Lili Wu, Weisheng Wu, Lulu Li, Wei Liu, Zheng Yan, Diankun Yu, Shengzhi Ren, Zhiqiang Hu, Xiaowen Li, Xiaowen Li, Xiaowen Li, Xiaowen Li

“…Therefore, it is essential to develop a diagnostic method with high sensitivity and specificity that can simultaneously detect and differentiate these viral and bacterial agents.Materials and methodsA triplex real-time PCR assay using TaqMan probes was developed to simultaneously detect PRV, PTV1, and SS2. …”
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Development and Application of a Multiplex Reverse Transcription–Droplet Digital PCR Assay for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus in Bivalve Shellfish by Maolin Wei, Jinfeng Wang, Yan Wang, Libing Liu, Xiangdong Xu, Jianchang Wang

“…In this study, a multiplex reverse transcription–droplet digital PCR (RT-ddPCR) assay based on the MS2 phage as a process control virus (PCV) was developed to achieve the simultaneous detection of hepatitis A virus (HAV) and hepatitis E virus (HEV) in bivalve shellfish. …”
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Development of a triplex TaqMan probe-based real-time PCR assay for simultaneous detection of swine influenza virus, porcine reproductive and respiratory syndrome virus, and porcine circovirus type 2 by MengJie Wang, Yu Pan, Wengjie Ma, Xingrong Wang, Changyou Xia, Yue Wang

“…Results To this end, we developed and validated a triplex TaqMan probe-based real-time PCR assay for the simultaneous detection and differentiation of SIV, PRRSV, and PCV2. …”
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Detecting Vietnamese fake news by Duc Vinh Vo, Phuc Do

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Detecting Vietnamese fake news by Duc Vinh Vo, Phuc Do

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Development and Validation of a Method for the Analysis of Multiple Pesticides in Fishery Products Using Gas Chromatography with Micro-Electron Capture Detection and Gas Chromatography–Tandem Mass Spectrometry by Myungheon Kim, Mihyun Cho, Changkyo Seo, Jaebin Im, Changhyeon Park, Yoonmi Lee, Mi-Ra Jo, Yong-Sun Moon, Moo-Hyeog Im

“…This study aims to develop a simultaneous analytical method for detecting 19 pesticides, including 4,4′-DDD, in fishery products using gas chromatography with micro-electron capture detection (GC-μECD) and gas chromatography–tandem mass spectrometry (GC-MS/MS). …”
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Review of respiratory syndromes in poultry: pathogens, prevention, and control measures by Huixin Liu, Sijia Pan, Chenchen Wang, Wenwen Yang, Xiaofang Wei, Yang He, Ting Xu, Kaichuang Shi, Hongbin Si

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Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement... by Nurul Affiqah Edora Mohd-Azhar, Haslawati Baharuddin, Mohamad-Sufiyan Salmi, Noor Faizah Ismail, Amatul-Samahah Md. Ali, Syazwan Saidin, Adibah Abu-Bakar

Subjects: “…Development of species-specific eDNA primer for the detection of the Asian arowana (Scleropages formosus)…”
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Development and validation of a lateral flow immunoassay using colloidal gold for the identification of feline herpesvirus type 1 by Jianzhong Wang, Jicheng Qiu, Yiduo Liu, Xianglin Ma, Xiaojie Wu, Xiaoguang Li, Wei Mao

“…In conclusion, the ICLF strip developed in this study represents a reliable, highly specific, and stable diagnostic tool for the rapid detection and management of FHV-1 infections in cats.…”
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Development of Smart pH-Sensitive Collagen-Hydroxyethylcellulose Films with Naproxen for Burn Wound Healing by Elena-Emilia Tudoroiu, Mădălina Georgiana Albu Kaya, Cristina Elena Dinu-Pîrvu, Lăcrămioara Popa, Valentina Anuța, Mădălina Ignat, Emilia Visileanu, Durmuș Alpaslan Kaya, Răzvan Mihai Prisada, Mihaela Violeta Ghica

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2016 ESC POSITION PAPER ON CANCER TREATMENTS AND CARDIOVASCULAR TOXICITY DEVELOPED UNDER THE AUSPICES OF THE ESC COMMITTEE FOR PRACTICE GUIDELINES by Editorial article

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EDUCATION ABOUT BREAST CANCER IN THE COMMUNITY AT NGLUYU HEALTH CENTER, NGANJUK by Gondo Mastutik, Dyah Fauziah, Anny Setijo Rahaju, Alphania Rahniayu, Ridholia, Nila Kurniasari, Etty Hari Kusumastuti, Khafidhotul Ilmiah

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Multi-class fruit ripeness detection using YOLO and SSD object detection models by Pooja Kamat, Shilpa Gite, Harsh Chandekar, Lisanne Dlima, Biswajeet Pradhan

“…Abstract Accurate fruit ripeness detection is critical to reducing post-harvest losses and improving quality control in agricultural systems. …”
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Rice Disease Detection: TLI-YOLO Innovative Approach for Enhanced Detection and Mobile Compatibility by Zhuqi Li, Wangyu Wu, Bingcai Wei, Hao Li, Jingbo Zhan, Songtao Deng, Jian Wang

“…As a key global food reserve, rice disease detection technology plays an important role in promoting food production, protecting ecological balance and supporting sustainable agricultural development. …”
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Simultaneous detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacteria in smear-negative sputum by a multiplex PCR assay: a clinical feasibility s... by Long Xie, Jing Cao, Yong Yang, Shao-Long Jiang, Jin-Lin Tan, Jia Zhang, Ze-Fan Ruan, Li Chen, Da-Yong Xu, Zhong Chen, Ming-Xiang Huang, Xi-Wen Jiang

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Detecting Important Features and Predicting Yield from Defects Detected by SEM in Semiconductor Production by Umberto Amato, Anestis Antoniadis, Italia De Feis, Anastasiia Doinychko, Irène Gijbels, Antonino La Magna, Daniele Pagano, Francesco Piccinini, Easter Selvan Suviseshamuthu, Carlo Severgnini, Andres Torres, Patrizia Vasquez

“…This study investigates the link between the defects detected by a Scanning Electron Microscope (SEM) and the electrical failure of the final semiconductors, with two main objectives: (a) to identify the best layers to inspect by SEM; (b) to develop a model that predicts electrical failures of the semiconductors from the detected defects. …”
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