Development of a multiplex loop-mediated isothermal amplification (LAMP) method for differential detection of Mycobacterium bovis and Mycobacterium tuberculosis by dipstick DNA chromatography by Mwangala L. Akapelwa, Thoko F. Kapalamula, Lavel C. Moonga, Precious Bwalya, Eddie S. Solo, Joseph Y. Chizimu, Jeewan Thapa, Kyoko Hayashida, Bernard M. Hang'ombe, Musso Munyeme, Aki Tamaru, Takayuki Wada, Shiomi Yoshida, Takuya Kodera, Mistuo Kawase, Stephen V. Gordon, Keiko Yamada, Chie Nakajima, Yasuhiko Suzuki

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Development of a technique for DNA detection and identification of toxigenic strains of Clostridium botulinum types A, B, E by the Real-Time PCR method by D. S. Yanov, A. V. Kuznetsovskiy, M. I. Dubrovin, A. V. Mironin, N. V. Onuchina, A. V. Filippov

“…The main obstacle to development of a technique for the detection and identification of DNA of toxigenic strains is the high variability of the genes responsible for the synthesis of botulinum toxin. …”
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Development of an Integrating Sphere-Based Wide-Range Light Source System for the Linearity Evaluation of a Photodetector Used in Radiation Detection and Bioanalysis Instruments by Tetsuro Matsumoto, Akihiko Masuda, Minoru Tanabe, Seiya Manabe, Hideki Harano, Kazuki Niwa

“…We developed a compact wide-range light source system for evaluating the linearity of photomultiplier tube (PMT) output. …”
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Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus by Liang Ma, Haoyan Zhu, Yongwei Jiang, Xiaomu Kong, Peng Gao, Yi Liu, Meimei Zhao, Guoxiong Deng, Yongtong Cao

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Topic Words-Based Multilingual Hateful Linguistic Resources Construction for Developing Multilingual Hateful Content Detection Model Using Deep Learning Technique by Naol Bakala Defersha, Kula Kekeba Tune, Solomon Teferra Abate

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Non-destructive detecting and annual duplicate verification of loquat fruit’s total soluble solids based on self-developed portable near-infrared spectrometer by WEI Yuqing, WANG Yuning, LI Shaojia, SUN Chongde, WU Di

“…A self-developed portable non-destructive quality detector was used to collect spectral data of ‘Baiyu’ loquat fruit harvested in 2018 and 2019, respectively, and then chemometric analysis methods were used to build detection model for total soluble solids (TSS) of loquat fruit. …”
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Development of an On-Column Trace Enrichment Method for the Determination of Sub-μg/L Bisphenol A in Bottled Water by RP-HPLC with Fluorescent Detection by Kevin C. Honeychurch

“…A simple extraction-free, on-column trace enrichment liquid chromatographic method for the determination of trace levels of bisphenol A (BPA) in bottled water samples has been developed. It was found possible to determine ng/L (ppt) levels of BPA by the direct introduction of 6 mL of sample water to the HPLC column utilising fluorescence detection (Exλ = 274 nm, Emλ = 314 nm). …”
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Development and Investigation of Diagnostic Efficiency of a Test Kit for the Detection of IgM-antibodies to Individual Antigens of Rubella Virus by Immunoblotting (Western Blot) by S. G. Mardanly, A. S. Avdonina

“…To date, in the Russian Federation, there is no such kit of reagents, which makes its development quite actual. Aim. The aim of this research was the development of a Russian test kit for detecting IgM-antibodies to individual rubella virus antigens by immune blotting (Western blot format). …”
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Development and characterization of gold nanoparticle-based colorimetric sensing assay functionalized with L-cysteine for the detection of calcium carbide in ripened mango and banana fruits by Aishwarya Dixit, Sonam Sonwal, Ashutosh Upadhyay, Vivek K. Bajpai, Yun Suk Huh, Shruti Shukla

“…Therefore, in the present study, a novel and cost-effective detection technique was developed for the detection of CaC2 using L-cysteine functionalized gold nanoparticles (AuNPs). …”
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Development of LC-MS/MS and GC-MS/MS Methods for the Detection of Ethyl Glucuronide (EtG) and Ethyl Palmitate (EtPa) in Hair by Sharnette Ashiru, Ethan Webster, Benjamin Barrett, Mathew Wade, Brian Rooney

“…In this paper, we present a validation process aimed at developing sensitive methods for detecting EtG and EtPa in hair samples. …”
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Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2 by Yun Hee Baek, Jihye Um, Khristine Joy C. Antigua, Ji-Hyun Park, Yeonjae Kim, Sol Oh, Young-il Kim, Won-Suk Choi, Seong Gyu Kim, Ju Hwan Jeong, Bum Sik Chin, Halcyon Dawn G. Nicolas, Ji-Young Ahn, Kyeong Seob Shin, Young Ki Choi, Jun-Sun Park, Min-Suk Song

“…With the recent emergence of the novel SARS-CoV-2, the availability of rapid, sensitive, and reliable diagnostic methods is essential for disease control. Hence, we have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the specific detection of SARS-CoV-2. …”
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Detecting Redundant Health Survey Questions by Using Language-Agnostic Bidirectional Encoder Representations From Transformers Sentence Embedding: Algorithm Development Study by Sunghoon Kang, Hyewon Park, Ricky Taira, Hyeoneui Kim

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DEVELOPMENT AND VALIDATION OF 3AB-ELISATEST-SYSTEM FOR DETECTION OF ANTIBODIES TO FMD VIRUS NONSTRUCTURAL PROTEINS IN BLOOD SERA FROM CATTLE AND SMALL RUMINANTS by A. S. Yakovleva, A. V. Kanshina, A. V. Scherbakov, Ye. S. Orlova

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Bridging the gap between the public’s knowledge and detection of marine non-indigenous species through developing automated image classification applications for marine species by Peng Zhou, Peng Zhou, Xue-Qing He, Zhi-Yi Tu, Dong Sun, Chun-Sheng Wang, Hong-Bin Shen, Xiaoyong Pan

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Development of a Triplex Real-Time PCR Method for the Simultaneous Detection of Porcine Circovirus 2, 3, and 4 in China Between 2023 and 2024 by Yanhong Chen, Yi Lu, Dongfan Li, Ling Dong, Yang Zeng, Zhijing Mei, Ahmed H. Ghonaim, USAMA, Zhixian Yu, Shuo Zhang, Ping Bai, Wentao Li, Xuexiang Yu, Qigai He

“…Results: A TaqMan triple real-time PCR method was developed, demonstrating excellent specificity, sensitivity, and repeatability, with limits of detection (LODs) of 53.3 copies/µL, 12.0 copies/µL, and 13.8 copies/µL for PCV2, PCV3, and PCV4, respectively. …”
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Non-invasive breath testing to detect colorectal cancer: protocol for a multicentre, case–control development and validation study (COBRA2 study) by Michael G. Fadel, James Murray, Georgia Woodfield, Ilaria Belluomo, Ivan Laponogov, Aaron Parker, Valerio Converso, James K. Ellis, Pete Wheatstone, Julie Hepburn, Chris Groves, Kevin Monahan, Brian P. Saunders, Patrik Španěl, Kirill Veselkov, Amanda J. Cross, Christos Kontovounisios, Linda D. Sharples, George B. Hanna, COBRA2 Collaborators

“…This protocol describes the COBRA2 study which aims to develop and validate the clinical prediction model (CPM) in the detection of CRC based on the breath test. …”
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Development of sensor based on electrochemical impedance spectroscopy for the detection of Vitamin D3 using molecularly imprinted polymer@MoSe2 nanosheets by A. Archana, Sandhya Rani Majhi, Harshulika Singh, Pratima R. Solanki

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Development and validation of a multiplex RT-qPCR method for the simultaneous detection of influenza type A, B and SARS-COV-2 viruses by Samira Karimkhani, Ehsan Lotfi, Fatemeh Karamali, Mahsa DarestaniFarahani, Reza Keikha, Mahmood Barati

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Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology. by R Hoare, K D Thompson, T Herath, B Collet, J E Bron, A Adams

“…They were also used to develop a novel detection method based on Luminex (Bio-Plex) bead-based flow cytometric technology for the detection of ISAV in the plasma of Atlantic salmon (Salmo salar L.) smolts experimentally infected with ISAV. …”
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Development of a Multiplex Real-Time PCR Assay for the Detection of Eight Pathogens Associated with Bovine Respiratory Disease Complex from Clinical Samples by Fuxing Hao, Chunhao Tao, Ruilong Xiao, Ying Huang, Weifeng Yuan, Zhen Wang, Hong Jia

“…In this study, a novel multiplex fluorescence-based quantitative polymerase chain reaction (qPCR) assay was developed for the simultaneous detection of eight major pathogens associated with BRDC. …”
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