Development and Validation of a Combined RT-LAMP Assay for the Rapid and Sensitive Detection of Dengue Virus in Clinical Samples from Colombia by Leidy Hurtado-Gómez, Katherine Escorcia-Lindo, Juan Sebastian Rosero, Nataly Solano Llanos, Camilo Barrios Sánchez, Anderson Díaz Pérez, Yirys Díaz-Olmos, Jennifer García, Yesit Bello-Lemus, Leonardo C. Pacheco-Londoño, Antonio J. Acosta Hoyos, Lisandro A. Pacheco-Lugo

“…<b>Objective</b>: This study focuses on the development and validation of a single-tube reverse transcription loop-mediated isothermal amplification termed TURN-RT-loop-mediated isothermal amplification (LAMP) for the detection of DENV. …”
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Correction: FilmArray, an Automated Nested Multiplex PCR System for Multi-Pathogen Detection: Development and Application to Respiratory Tract Infection

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Development and synthesis of DNA-based label-free electrochemical biosensor for detection of Enterocytozoon bieneusi using screen-printed gold electrode by Sara Nemati, Amir Sadeghi, Seyyed Mehdi Khoshfetrat, Hanieh Mohammad Rahimi, Kobra Omidfar, Hamed Mirjalali

“…In this study, a label-free electrochemical genosensor was developed to detect Enterocytozoon bieneusi using specific probe and electrode substrate, Mxene-Ti3C2/AuNPs/PDA. …”
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Developing a Vital Signal Detection Electrode for Fabric Substrate Using a High-Performance Conductive Carbon-Based Ink by K. Chansaengsri, B. Tunhoo, K. Onlaor, T. Thiwawong

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Development of a method for measuring mass concentration of cyanides in soil samples using gas chromatography and tandem mass-selective detection by D. O. Korneev, M. A. Ponsov, A. A. Rodionov, L. V. Petrakova, I. V. Rybalchenko

“…The developed method involves the use of 2,3,4,5,6-pentafluorobenzyl bromide as a derivatizing agent and GC/MS/MS with methane negative ion chemical ionization. …”
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Development of an Immunochromatographic Test with Recombinant MIC2-MIC3 Fusion Protein for Serological Detection of <i>Toxoplasma gondii</i> by Jianzhong Wang, Yi Zhao, Jicheng Qiu, Jing Liu, Rui Zhou, Xialin Ma, Xiaojie Wu, Xiaoguang Li, Wei Mao, Yiduo Liu, Heng Zhang

“…This study aimed to design, develop, and evaluate a novel immunochromatographic test (ICT) using a recombinant MIC2-MIC3 fusion protein (rMIC2-MIC3) for detecting specific antibodies against <i>T. gondii</i>. …”
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Development and feasibility testing of an AI-powered chatbot for early detection of caregiver burden: protocol for a mixed methods feasibility study by Ravi Shankar, Anjali Bundele, Amanda Yap, Amartya Mukhopadhyay

“…Twenty primary caregivers of ESKD patients will be recruited to use BOTANIC for 12 weeks. BOTANIC, developed using Python and open-source libraries, will integrate with Telegram and utilize advanced NLP techniques to analyze caregiver conversations and detect signs of burden. …”
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Development of ELISA Using Phage-Displayed Stx2 Mini-Body for Detection of STEC Antigen in Field Farming Pig Samples by Jin Hur, Ho-Kyoung Jung, Jung-Ho Park, Anoth Maharjan, Seung-Won Park

“…The ELISA values of positive stool samples were >300 pg and <600 pg. In conclusion, we developed an innovative ELISA for the detection of STEC antigens in field pig farming samples. …”
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Development of polymerase chain reaction kit for detection of SARS-CoV-2 RNA in biological samples collected from animals by A. M. Timina, A. S. Yakovleva, M. V. Timanov, M. V. Biryuchenkova, Ye. S. Orlova

“…Today the key methodofCOVID-19 diagnosis is polymerase chain reaction, butall currently availabletest-kits are intended for the virus detection in humans. The paper demonstrates data on the development of thereal-timePCR-basedmethodforSARS-CoV-2 RNA detection in the biological samples collected from animals. …”
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Development of a rapid on-site detection method for largemouth bass virus based on RPA-CRISPR/Cas12a system by Na Li, Chunmei Dai, Yuelin Mao, Yimeng Zhang, Huiyuan Yan, Huijiao Wang, Yinghui Qin, Baicheng Huang, Xinjie Wang, Lunguang Yao

“…Rapid, simple, and reliable detection methods are essential for the timely identification of LMBV infections, enabling effective prevention and control measures.MethodsIn this study, a detection platform utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 12a (Cas12a) system was developed for LMBV. …”
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Development of a Rapid Epstein–Barr Virus Detection System Based on Recombinase Polymerase Amplification and a Lateral Flow Assay by Yidan Sun, Danni Tang, Nan Li, Yudong Wang, Meimei Yang, Chao Shen

“…In our research, we developed EBV detection systems: (1) a polymerase chain reaction (PCR)-based detection system, (2) a recombinase polymerase amplification (RPA)-based detection system, and (3) a combined RPA-lateral flow assay (LFA) detection system. …”
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A Laboratory-Developed Assay for the Simultaneous Detection of <i>Aspergillus fumigatus</i> and <i>Pneumocystis jirovecii</i> Pulmonary Pathogens by Margherita Cacaci, Debora Talamonti, Giulia Menchinelli, Damiano Squitieri, Riccardo Torelli, Elena De Carolis, Giulia De Angelis, Maurizio Sanguinetti, Brunella Posteraro

“…This study describes the development and validation of a real-time PCR-based laboratory-developed assay (LDA) on the Panther Fusion system for the simultaneous detection of <i>Aspergillus fumigatus</i> (AF) and <i>Pneumocystis jirovecii</i> (PJ) in bronchoalveolar lavage fluid (BALF) samples. …”
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Development of a Synthetic VP1 Protein Peptide-Based ELISA to Detect Antibodies Against Porcine Bocavirus Group 3 by Chao Gong, Hui He, Yuguang Fu, Baoyu Li, Bin Yang, Jianlong Li, Xiaodong He, Juncheng Han, Yi Zhang, Guangliang Liu, Qingyong Guo

“…Currently, effective serological methods for the detection of antibodies against PBoV G3 are limited. …”
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Ultrasound-detected osteophytes predict the development of radiographic and clinical features of hand osteoarthritis in the same finger joints 5 years later by Tore K Kvien, Hilde Berner Hammer, Ida K Haugen, Barbara Slatkowsky-Christensen, Alexander Mathiessen

“…Significant associations were found between ultrasound-detected osteophytes and development of both radiographic OA (OR=4.1, 95% CI 2.0 to 8.1) and clinical bony enlargement (OR=3.5, 95% CI 2.4 to 5.1) and also incident radiographic osteophytes (OR=4.2, 95% CI 2.1 to 8.5) and JSN (OR=5.3, 95% CI 2.1 to 13.4).Conclusion Ultrasound-detected osteophytes predicted incident radiographic and clinical hand OA 5 years later. …”
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Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA by Yinhua Zhang, Nathan A Tanner

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Development of a Visual Assay for Detection of Viable <i>Cronobacter sakazakii</i> Using RT-PSR and Hydroxynaphthol Blue Indicator by Peng Wang, Qiming Chen, Yikai Wang, Xueting Sun, Zhanmin Liu

“…<i>Cronobacter sakazakii</i> is a foodborne pathogen in powdered infant formula, which poses a significant risk to susceptible populations such as infants and the elderly. This study aims to develop a visual detection method for viable <i>C. sakazakii</i> using the reverse transcription-polymerase spiral reaction and hydroxynaphthol blue indicator. …”
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Development of real-time Internet of Things motion detection platform applying non-contact sensor based on open source hardware by Jung Woo Kim, Sang Hun Sul, Jae Boong Choi

“…The real-time Internet of Things motion detection platform has been developed to detect and monitor users in installed spaces. …”
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Development of a Multiplex TaqMan Assay for Rapid Detection of Groundnut Bud Necrosis Virus: A Quarantine Pathogen in the USA by Anushi Suwaneththiya Deraniyagala, Avijit Roy, Shyam Tallury, Hari Kishan Sudini, Albert K. Culbreath, Sudeep Bag

“…For the rapid and accurate detection of GBNV at points of entry, TaqMan reverse transcriptase–quantitative polymerase chain reaction (RT-qPCR) assays were developed and the results validated using conventional reverse transcriptase–polymerase chain reaction (RT-PCR) followed by Sanger sequencing. …”
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Capillary Electrophoresis with Diode Array Detection for the Quantification of Triptorelin and Lanreotide in Pharmaceutical Quality Control: Development, Validation, Greenness and Practicality Evaluation by Stefanik O., Mikus P., Piestansky J.

“…Therefore, this study aimed to develop a novel, green, and simple hydrodynamically open-system capillary zone electrophoresis method with diode-array detection (CZE-DAD) for the simultaneous analysis of lanreotide and triptorelin in a single electrophoretic run and to objectively evaluate the analytical technique’s greenness and practicality for application in the pharmaceutical QC settings.…”
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Development of an H&E on-block staining technique for collagen detection in cryo-fluorescence tomography imaging of frozen breast tissue samples by Erin P. Snoddy, Tien T. Tang, Natalie Fowlkes, Thomas Huynh, Kari J. Brewer Savannah, Alejandro Contreras, Gregory Reece, Kristy K. Brock

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