The development and validation of a microneutralization assay for the detection and quantification of anti-yellow fever virus antibodies in human serum by Katherine Fries, Ping Luo, Rebekah Baldwin, Raechel Goldberg, Ivan Ordonez, Lingyi Zheng, James Huleatt, Louis Devlin

“…Here, we describe the development and validation of a new Vero cell-based YF microneutralization (MN) assay, with immunostaining readout, for the detection and quantification of YF virus-neutralizing antibodies in human serum. …”
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Development and validation of Burkholderia pseudomallei-specific real-time PCR assays for clinical, environmental or forensic detection applications. by Erin P Price, Julia L Dale, James M Cook, Derek S Sarovich, Meagan L Seymour, Jennifer L Ginther, Emily L Kaufman, Stephen M Beckstrom-Sternberg, Mark Mayo, Mirjam Kaestli, Mindy B Glass, Jay E Gee, Vanaporn Wuthiekanun, Jeffrey M Warner, Anthony Baker, Jeffrey T Foster, Patrick Tan, Apichai Tuanyok, Direk Limmathurotsakul, Sharon J Peacock, Bart J Currie, David M Wagner, Paul Keim, Talima Pearson

“…Currently available molecular assays for B. pseudomallei detection lack rigorous validation across large in silico datasets and isolate collections to test for specificity, and none have been subjected to stringent quality control criteria (accuracy, precision, selectivity, limit of quantitation (LoQ), limit of detection (LoD), linearity, ruggedness and robustness) to determine their suitability for environmental, clinical or forensic investigations. …”
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Development of an efficient mixed mode heart disease detection model using machine learning and deep learning techniques by Reddy C. Ravi Shankar, A Niranjan, Kumari P.V. Gopi, Rajendra Babui Y.

“…This is probing the researchers for development of accurate and efficient diagnostic systems. …”
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Development of a highly sensitive reporter gene cell line for detecting estrogenic activity (the ER Isjaki assay) by Aline Colonnello Montero, Geeta Mandava, Agneta Oskarsson, Johan Lundqvist

“…In this study, the optimal experimental conditions for developing a novel and highly sensitive reporter gene assay were established. …”
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Development of a Key Method for the Optimization of Port Vessel Detection Based on an Improved Multi-Structural Morphology Approach by Bernard Marie Tabi Fouda, Wenjun Zhang, Jacques Atangana, Helene Carole Edima-Durand

“…It is known that some vessels do not comply with maritime rules, particularly in port and safety zones; these must be detected to avoid incidents. For these reasons, in this study, we propose and develop an improved multi-structural morphology (IMSM) approach to eliminate all of this noise and interference so that vessels can be accurately detected in real time. …”
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Developing a Prediction Model for Real-Time Incident Detection Leveraging User-Oriented Participatory Sensing Data by Md Tufajjal Hossain, Joyoung Lee, Dejan Besenski, Branislav Dimitrijevic, Lazar Spasovic

“…Effective incident detection is essential for emergency response and transportation management. …”
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Development of a duplex ddPCR assay for detection of the endangered European eels in the diet of the invasive European catfish by Castagné Paul, Martignac François, Santoul Frédéric, Blanchet Simon, Loot Géraldine

“…Here, we develop a ddPCR assay to detect predation events of a native endangered fish species, the European eel (Anguilla anguilla) from stomach content of an invasive predator, the catfish (Silurus glanis). …”
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Development of high sensitive real-time PCR to detect mustard and other allergens of the family Brassicaceae in food samples by K. A. Kurbakov, V. N. Zhulinkova, M. Yu. Minaev

“…As mustard is an allergen, it is necessary to control its presence. The development of PCR test-systems for its detection is complicated by the fact that this condiment can be made from seeds of various plant species (Brassica juncea, Brassica nigra, Sinapis alba) of the family Brassicaceae that are not closely related. …”
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Development of a Grape Cut Point Detection System Using Multi-Cameras for a Grape-Harvesting Robot by Liangliang Yang, Tomoki Noguchi, Yohei Hoshino

“…To reduce the labor force for the harvesting job, in this study, we developed a robot harvester for the vine grapes. In this paper, we proposed an algorithm that using multi-cameras, as well as artificial intelligence (AI) object detection methods, to detect the thin stem and decide the cut point. …”
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Development of Polydopamine–Chitosan-Modified Electrochemical Immunosensor for Sensitive Detection of 7,12-Dimethylbenzo[a]anthracene in Seawater by Huili Hao, Chengjun Qiu, Wei Qu, Yuan Zhuang, Zizi Zhao, Haozheng Liu, Wenhao Wang, Jiahua Su, Wei Tao

“…Therefore, the development of novel, efficient detection techniques is imperative. …”
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Development and performance analysis of a PIPS-based detection system for ultra-low Strontium-90 measurement in seawater by Younghak Kim, Hyuncheol Kim, Mee Jang, Jaehyeon Seo, Gahyun Kim, Hyemi Cha

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The Development of TIM-Barrel Based Multi-Epitope Protein for <i>Toxoplasma gondii</i> Serological Detection in Cats by Preeyanuch Thongpoo, Jiravich Methawiroon, Bandid Mangkit, Rucksak Rucksaken, Metita Sussadee, Warin Rangubpit, Sasimanas Unajak, Sathaporn Jittapalapong, Eukote Suwan

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Development of Enzyme-Mediated Duplex Exponential Amplification Assay for Detection and Identification of <i>Meloidogyne enterolobii</i> in Field by Bingxue Sun, Bo Gao, Rongyan Wang, Shulong Chen, Xiuhua Li, Yonghao Dong, Juan Ma

“…We thus developed a method for detecting <i>M. enterolobii</i> based on enzyme-mediated duplex exponential amplification (EmDEA) technologies to address this issue. …”
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Development of a Fully Automated Microfluidic Electrochemical Sensor on the ESSENCE Platform for Rapid Detection of Single-Stranded DNA by Niranjan Haridas Menon, Maryom Rahman, Sagnik Basuray

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Development of a Gas Chromatography-Time-of-Flight Method for Detecting Glucosinolate Metabolites and Volatile Organic Compounds in Kimchi by Ho Jin Kim, Mi Jin Lee, Suel Hye Hur, Min Hee Jeong

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Development of a SYBR Green-Based RT-qPCR Assay for the Detection and Quantification of Lone Star Virus by Megan Burch, Jeremy Bechelli

“…Two SYBR green-based RT-qPCRs were developed and validated for the detection and quantification of LSV. …”
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Development of an In-Field Method for the Detection of Barium in Various Water Samples Using Differential Pulse Anodic Stripping Voltammetry by S. Ridgway, M. Wajrak

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Development and Validation of a UHPLC-MS/MS Method for Ciprofol Detection in Plasma: Application in Clinical Pharmacokinetic Studies by Zhu J, Cao Y, Zhang X, Xu B

“…The flow rate was maintained at 0.4 mL·min⁻¹, and the column temperature was set at 40°C. Detection was performed using electrospray ionization (ESI) in negative ion mode with multiple reaction monitoring (MRM). …”
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Descriptive Report of Microorganisms Detected in Cultures of COVID-19 Patients Developing Secondary Infection After Tocilizumab Use by Dena Firouzabadi, Sahar DehghanKhalili, Saeed DehghanKhalili

“…Seventy-seven patients (74.8%) in the tocilizumab group developed secondary infection, irrespective of the culture origin. …”
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An alternative colorimetric RT-LAMP assay for the rapid detection of SARS-CoV-2: development and validation in Thailand by Wiphat Klayut, Thanee Wongchai, Nattagarn Chuenchom, Payu Bhakdeenuan, Supranee Bunchoo, Sopa Srisungngam, Sirilada Suphankong, Benjawan Phetsuksiri, Ballang Uppapong, Janisara Rudeeaneksin

“… Introduction: COVID-19, an emerging infectious disease caused by SARS-CoV-2, continues to be a global public health threat. The development of a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) can extend the availability of simple, reliable molecular tests for the rapid detection of COVID-19. …”
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