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Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens
Published 2025-12-01“…Furthermore, we collected 676 chicken samples from an established infection model to compare the performance of the TaqMan qPCR assay with traditional bacterial culturing and antibody-based detection approaches. With superior sensitivity and specificity, the newly developed method detected over 80% of positive chickens, significantly outperforming the two conventional methods. …”
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282
Development of a sugar-derived silver nanoparticle-based electrochemical immunosensor for sensitive D-dimer detection
Published 2025-01-01“…Here, we describe the development of a simple electrochemical immunosensor to detect DD. …”
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283
Development of Au-Nanoprobes Combined with Loop-Mediated Isothermal Amplification for Detection of Isoniazid Resistance in Mycobacterium tuberculosis
Published 2016-01-01“…Further, it does not require thermal cycles for MDR-TB detection.…”
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Development of an Improved Thiosulfate-Utilizing Denitrifying Bacteria-Based Ecotoxicity Test with High Detection Sensitivity and Reproducibility
Published 2024-10-01“…The present study aimed at developing a thiosulfate-utilizing denitrifying bacteria (TUDB)-based ecotoxicity test with high detection sensitivity and favorable reproducibility. …”
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287
Development of Dual ‘RT‐LAMP‐LFA’ Rapid Detection Technology With Gold Magnetic Nanoparticles for Influenza Virus
Published 2025-06-01“…This study developed a dual ‘RT‐LAMP‐LFA’ detection technology with gold magnetic nanoparticles for influenza virus. …”
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288
Development of Low-Stress Double-Pass Filter Device for Methane and Ethane Flammable Gas Detection System
Published 2024-10-01“…To meet the needs of industrial and environmental monitoring of methane and ethane flammable gases, a low-stress filter device for detecting methane and ethane simultaneously is developed based on the absorption spectra of methane and ethane and the thin-film design theory. …”
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Development of a Point-of-Care Immunochromatographic Lateral Flow Strip Assay for the Detection of Nipah and Hendra Viruses
Published 2025-07-01“…Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. …”
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291
Development of an internalin-based double-antibody sandwich quantitative ELISA for the detection of Listeria monocytogenes in slaughterhouse environments
Published 2025-03-01“…To enable the specific, rapid, and cost-effective detection of L. monocytogenes in environments and animal-based food, we developed a double-antibody sandwich quantitative ELISA (DAS-qELISA) method.MethodsThe method is based on monoclonal antibodies targeting internalin G (InlG), a surface protein of L. monocytogenes with demonstrated immunogenicity. …”
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Development and evaluation of fluorescent recombinase polymerase amplification (RPA)-based method for rapid detection of Necator americanus.
Published 2025-04-01“…Although several molecular assays are available for N. americanus, they have limitations in detecting mild N. americanus infections.<h4>Methods</h4>Fluorescent RPA primers and probes targeting the N. americanus internal transcribed spacer 2 (ITS2) gene were developed. …”
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Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection
Published 2021-07-01“…Furthermore, a quadruplex RT-PCR system was developed that could differentiate ToBRFV from other economically important viruses affecting tomatoes, such as TMV, tomato mosaic virus, and tomato spotted wilt virus. …”
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Development of a thrombin-antithrombin complex detection kit and study in venous thromboembolism complicated by cervical cancer
Published 2024-12-01“…Abstract Objective Our study successfully developed an assay kit for thrombin-antithrombin complex (TAT) and demonstrated the predictive value of plasma TAT concentration in the development of venous thromboembolism (VTE) in patients with cervical cancer. …”
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Field applicable all-in-one kit developed for rapid detection of ‘Candidatus Liberibacter asiaticus’ in plants and psyllids
Published 2025-07-01“…The applicability of the All-in-one kit for CLas detection was confirmed using plant or psyllid crude DNA extracts. …”
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SOFTWARE COMPONENT DEVELOPMENT FOR PARALLEL GATEWAYS DETECTION AND QUALITY ASSESSMENT IN BPMN MODELS USING FUZZY LOGIC
Published 2025-07-01“…The software component is developed using Python, as well as third-party libraries: Pandas, NumPy, and Matplotlib. …”
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Development and application of a whole transcriptome sequencing assay for the detection of gene fusions in clinical cancer specimens
Published 2025-05-01“…Methods We developed a novel WTS-based assay for the detection of gene fusions, MET exon 14 skipping and EGFR VIII alterations in clinical samples. …”
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