Development and validation of mPCR-CEFA for detecting multiple deletion and non-deletion thalassemia genotypes by Jingping Xu, Jingping Xu, Baoyan Ren, Qixun Fang, Kangfeng Lin, Kangfeng Lin, Xingan Xing, Xingan Xing, Jingting Lin

“…Rapid and accurate genotyping is essential for molecular screening and prenatal genetic diagnosis to prevent the birth of individuals with severe forms of the disease.MethodsWe developed a multiplex PCR-capillary electrophoresis fragment analysis (mPCR-CEFA) method to detect 16 α-thalassemia and 24 β-thalassemia genotypes simultaneously. …”
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Development of Polyphenol–Metal Film-Modified Colored Porous Microspheres for Enhanced Monkeypox Antigen Detection by Wei-Zhi Zhang, Chen-Fei Zhang, Shou-Nian Ding

“…In this article, we developed a novel class of colored microspheres designed for application in a lateral flow immunoassay (LFIA) platform targeting MPXV-specific biomarkers. …”
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Development of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air by Tomoyo Yoshinaga, Yoshinori Ando, Yumi Sato, Takeru Kishida, Masaaki Kitajima

“…Abstract Several studies have successfully detected SARS-CoV-2 in air samples. However, most of these studies focused on validating the air collection method, and there was no report on the development of a virus detection method. …”
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Push-Up Detector Applications Using Quality Function Development and Anthropometry for Movement Error Detection by Ari Muzakir, Christofora Desi Kusmindari

“…In this way, nonstandard movements can be detected and corrected immediately. It has two motion sensors integrated with Arduino-based microcontroller. …”
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Development of a Smart System for Early Detection of Forest Fires based on Unmanned Aerial Vehicles by Georgi Hristov, Nikolay Zlatov, Plamen Zahariev, Chi Hieu Le, Diyana Kinaneva, Georgi Georgiev, Yavor Yotov, James Gao, Anh My Chu, Ho Quang Nguyen, Le Minh Huynh, Trung Thanh Bui, Mohd Nor Azmi Ab Patar, Jamaluddin Mahmud, Michael S. Packianather

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Detection of Gallbladder Disease Types Using a Feature Engineering-Based Developed CBIR System by Ahmet Bozdag, Muhammed Yildirim, Mucahit Karaduman, Hursit Burak Mutlu, Gulsah Karaduman, Aziz Aksoy

“…<b>Background/Objectives:</b> Early detection and diagnosis are important when treating gallbladder (GB) diseases. …”
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Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp. by Satyaki Ghosh, David L Straus, Christopher Good, Vipaporn Phuntumart

“…This study is the first to report the development of loop-mediated isothermal amplification (LAMP) for the detection of Saprolegnia spp. and compares it with quantitative PCR (qPCR). …”
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Comparative analysis of single-board computers for the development of a microarchitectural computing system for fire detection by D. A. Paulenka

“…Despite the fact that Raspberry Pi 4 Model B meets the terms of reference to the project as a computational basis, when developing an on-board microarchitectural computing system for detecting anomalous situations, it is worth using more powerful alternatives with built-in AI-accelerators (e.g., Radxa Rock 5 Model A) or with an additional external AI-accelerator (e.g., a combination of Cool Pi 4 Model B and Google Coral USB Accelerator). …”
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Development of a simple and quick immunochromatography method for detection of anti-HPV-16/-18 antibodies. by Fumiko Endo, Tsutomu Tabata, Daichi Sadato, Machiko Kawamura, Noriyuki Ando, Keisuke Oboki, Masako Ukaji, Kaoru Kobayashi, Yukuharu Kobayashi, Tomoaki Ikeda, Futoshi Shibasaki

“…In this study, we established an IC method to detect serum antibodies against oncogenic human papillomavirus (HPV)-16 and HPV-18 L1 proteins using recombinant L1 proteins produced by silkworms as antigens. …”
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Development and Validation of Approach for the Detection of Neutralizing Antibodies Against Insulin (Glargine) in Human Blood Plasma by N. B. Abramenko, P. I. Vnukova, E. S. Golovina, I. E. Makarenko, A. A. Mosikian, A. G. Nikiforova, P. V. Gremyakova, V. I. Kazey

“…Applying of cell-based methods one allows to determinate neutralizing antibodies to insulin.Aim. Development and validation methods for detection of neutralizing antibodies against insulin in human plasma.Materials and methods. …”
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Spatial Spillover Effect and Boundary Detection of County Tourism Economic Growth Promoting Economic Development by Liu Anle, Yang Chengyue, Ming Qingzhong, Wu Fenglin

“…Based on the Tourism Economic Growth Hypothesis and the "First Law of Geography" hypothesis, using panel data from 88 county units in Guizhou Province from 2010 to 2020, this study tested the spatial spillover effect of county tourism economic growth on economic development based on the spatial panel Durbin model double fixed effect model and detected its spatial attenuation boundary. …”
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Development of a toxin-selective immunotracer for in vivo detection of Clostridioides difficile infection by immunoPET by Mario González-Arjona, Lorena Cussó, Luis Alcalá, María Isabel González, Alexandra de Francisco, María Jesús Fernández-Aceñero, Dag Sehlin, Stina Syvänen, Emilio Bouza, Patricia Muñoz, Manuel Desco, Beatriz Salinas

“…Conclusions We successfully developed an immunoPET radiotracer targeting toxin B for detection of CDI. …”
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Development of A Real-Time PCR Assay for Plasmodiophora brassicae and Its Detection in Soil Samples by Jin-ping LI, Yan LI, Yan-xia SHI, Xue-wen XIE, Chai A-li, Bao-ju LI

“…A SYBR Green I real-time PCR assay was developed to detect and quantify Plasmodiophora brassicae ribosomal DNA (rDNA) and internal transcribed spacer (ITS). …”
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Development and Validation of RAA-CRISPR/Cas12a-Based Assay for Detecting Porcine Rotavirus by Siyu Huang, Longhuan Du, Song Liu, Qingcheng Yang, Changwei Lei, Hongning Wang, Liu Yang, Xin Yang

“…Subsequently, we developed a rapid PoRV detection method using a combination of recombinase-aided amplification (RAA) and CRISPR/Cas12a. …”
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Development of a New Indirect ELISA Test for the Detection of Anti-Feline Coronavirus Antibodies in Cats by Irene Ferrero, Sarah Dewilde, Paolo Poletti, Barbara Canepa, Enrica Giachino, Paola Dall’Ara, Joel Filipe

“…Among serological tests, the indirect immunofluorescent antibody test (IFAT), considered the gold standard, and the enzyme-linked immunosorbent assay (ELISA) are the most widely used to detect FCoV antibodies. The aim of this work was the development of FCoVCHECK Ab ELISA, a new rapid indirect test for the detection of FCoV antibodies in feline serum/plasma samples. …”
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MXene-Based Gas Sensors for NH₃ Detection: Recent Developments and Applications by Yiyang Xu, Yinglin Wang, Zhaohui Lei, Chen Wang, Xiangli Meng, Pengfei Cheng

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Application of change detection techniques driven by expert opinions for small-area studies in developing countries by Tanaka A. Mbendana, Anesu D. Gumbo, Simbarashe Jombo, Ephias Mugari, Evison Kapangaziwiri

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Development of a Novel, Non-Invasive Saliva Sampling Method for the Detection of Bovine Respiratory Viruses by Simona Baumann, Belinda Euring, Maxi Harzer, Mandy Eibisch, Andrea Lindner, Thomas W. Vahlenkamp, Kristin Heenemann

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Molecular detection and identification of Trichobilharzia: development of a LAMP, qPCR, and multiplex PCR toolkit by Jan Procházka, Zikmund Bartoníček, Roman Leontovyč, Petr Horák, Tomáš Macháček

“…Methods Tricho-LAMP and Tricho-qPCR were designed and optimised for Trichobilharzia DNA detection. A multiplex PCR assay was also developed and optimised to identify the three main species causing CD in Europe (Trichobilharzia franki, T. szidati, and T. regenti). …”
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The Influence of Sports Dance on the Physical and Mental Development of Contemporary College Students Based on Health Detection by Huali Huang

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