Exploring the functional role of POU5F1 using CRISPR RNP electroporation of zygote (CRISPR-EZ) in buffaloes by Meeti Punetha, Dharmendra Kumar, Suman Choudhary, Sheetal Saini, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, P. S. Yadav, T. K. Datta

“…In conclusion, direct electroporation of the CRISPR RNP component to the earlier stage of the zygote efficiently created mutations in the POU5F1 gene. We developed an easy and straightforward protocol for gene editing, which could serve as a valuable method for studying the functional genomics of the buffalo embryos. …”
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Assessment of Minimum Miscibility Pressure for CO2 Injection by an Enhanced Peng-Robinson Equation of State using Different Approaches. A Case Study for an Iraqi Oil Field by Rusul H. Rabeeah, Mohammed S. Al-Jawad

“…There are many methods, such as experimental methods, that are accurate yet costly and time-consuming for determining the MMP; thus, researchers often turn to mathematical techniques, including equations of state and empirical correlations. …”
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FBMC vs. PAM and DMT for High-Speed Wireline Communication by Jeremy Cosson-Martin, Jhoan Salinas, Hossein Shakiba, Ali Sheikholeslami

“…Second, the data rate is determined using lab-measured results. And third, the performance when subject to notched channels is analyzed using simulation results. …”
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Design Process Framework for the Definition and Conceptual Design of Structurally Transforming Multi-Mode Products by Lark Sawyer, Jonathan Luntz, Kara Stark, Meghan Mojica, Karen Williams

“…The Problem Definition stage in this new framework involves identifying the external factor that determines the product’s modes and defining the functional requirements for the modes and transformation methods. …”
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Selection of the End-Expiratory Pressure for Mechanical Respiratory Support (Review) by R. Y. Ovsiannikov, K. M. Lebedinskii

“…The absence of «gold standard» for end-expiratory pressure optimization together with its obvious significant contribution to the efficiency and safety of respiratory support has driven the search for the optimal method of choosing its values for several decades.Aim of the review: to identify the optimal methods for determining the values of end-expiratory pressure based on the analysis of its positive and negative effects in the used strategies of mechanical respiratory support.Material and methods. …”
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PRACTICAL APPLICATION OF CLASSIFICATION OF PATHOLOGICAL POSTOPERATIVE SCARS OF FACE by Y. Melnychuk, R. Ogonovskyi, A. Kucher, A. Filipskyi

“…The results prove the practical advisability of the proposed classification and efficiency of own methods of early prevention of postoperative pathological scars formation of skin, which consist of complex application of extracorporeal shock wave therapy combined with Gel Strataderm Conclusions. …”
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Control system architecture of an intelligent agent based on a semiotic network by M. A. Rovbo, P. S. Sorokoumov

“…Therefore, the developed method can improve the efficiency of education. From the technical point of view, the relevance of the work is in the contribution to the development of an approach to controlling groups of robots or software agents with elements of social structures. …”
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Software lifecycle management based on the OMG Essence standard by Andrii Zelenkov, Ludmila Mandrikova, Yevhen Borovkov

“…The goal is to enhance the efficiency of team-based software development and to foster the advancement of practices, methods, and methodologies in software engineering. …”
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Effects of Commercial Probiotics on Sperm Traits, Intestinal Morphology, Gut Microflora, and Antioxidant Status in 65-Week-Old Ross 308 Broiler Breeder Roosters by Arash Hadavi, Farogh Kargar, Najeebullah Fayaz, Ali Tayebi pour, Muhammad Namazi zadgan

“…Sperm characteristics were assessed by collecting samples using the abdominal rub method every ten days. The eosin and nigrosine staining method determined the percentage of live and dead sperm. …”
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Response of Anti-Oxidative Stress Genes in Liver and Intestine in Broilers Fed Organic Acid and Silver Nanoparticles Coated on Zeolite in Starter Period by Fatemeh Asgharnezhad, Seyed Reza Hashemi, Saeed Hassani, Sharif Rostami

“…Extended Abstract Background: The use of food supplements to improve growth, optimal production and increase feed efficiency in feeding livestock and poultry has become a common thing as a suitable solution in using feed as well as possible. …”
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Bright Luminal Sign on High b-Value Diffusion-Weighted Magnetic Resonance Enterography Imaging as a New Biomarker to Predict Fibrotic Strictures in Crohn’s Disease Patients: A Retr... by Luca Pio Stoppino, Stefano Piscone, Ottavia Quarta Colosso, Sara Saccone, Paola Milillo, Nicola Della Valle, Rodolfo Sacco, Alfonso Reginelli, Luca Macarini, Roberta Vinci

“…These findings indicate that DWI hyperintensity can be considered as a good non-invasive indicator for the detection of severe intestinal fibrosis and may provide an efficient and accurate method for assessing fibrotic strictures. …”
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The concept of industrial quasi-market: Tracing the research programme evolution by Oksana V. Komarova, Natalya Yu. Yaroshevich

“…The findings refine the theoretical understandings of the industrial markets’ development, primarily of complex technological products, and allow designing methods for measuring the efficiency of market structures as well as industrial policy methods.…”
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Attention-based BiLSTM-XGBoost model for reliability assessment and lifetime prediction of digital microfluidic systems by Lifeng He, Qili Yang, Junxi Chen, Wenjing Liu, Zhijie Luo

“…The model achieved a mean absolute percentage error (MAPE) of 1.6464, Root mean squared error (RMSE) of 0.3667, mean absolute error (MAE) of 0.2557, and a coefficient of determination (R-squared) of 0.9949. Compared to baseline methods, the proposed BiLSTM-XGBoost model achieves the highest prediction accuracy, enabling effective health monitoring, problem identification, and failure prediction. …”
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Проблематика оценки эффективности государственного финансирования социальных инвестиционных проектов...

“…The conclusion about the need to use in determining the discount rate, a combined approach based on the consistent application of methods of economic-mathematical modeling and experimental evaluation obtained for a series ofsuccessiveperiods of the values of standard efficiency investments of federalfunds in the investment program of the regions.…”
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Methodology for forecasting average long-term damage in the economic justification of flood protection measures by Andrey V. Shalikovsky, Sergey G. Kosarev, Konstantin A. Kurganovich, Maxim A. Bosov, Alla V. Maslova, Evgenia Kh. Zykova, Alexey A. Solodukhin, Denis V. Kochev

“…An increase in damage from floods suggests the need to implement economically sound preventive measures, but currently in Russia, there are no approved methods for calculating the economic efficiency of water management measures. …”

Natural Deep Eutectic Solvents (NADESs) for the Extraction of Bioactive Compounds from Quinoa (<i>Chenopodium quinoa</i> Willd.) Leaves: A Semi-Quantitative Analysis Using High Per... by Verónica Taco, Dennys Almachi, Pablo Bonilla, Ixchel Gijón-Arreortúa, Samira Benali, Jean-Marie Raquez, Pierre Duez, Amandine Nachtergael

“…Given the interest in flavonoids from <i>Chenopodium quinoa</i> Willd. leaves, an efficient “green” extraction method was developed by investigating eight NADESs with defined molar ratios, i.e., malic acid-choline chloride (chcl)-water (w) (1:1:2, N1), chcl-glucose-w (5:2:5, N2), proline-malic acid-w (1:1:3, N3), glucose-fructose-sucrose-w (1:1:1:11, N4), 1,2-propanediol-chcl-w (1:1:1, N5), lactic acid-glucose-w (5:1:3, N6), glycerol-chcl-w (2:1:1, N7), and xylitol-chcl-w (1:2:3, N8). …”
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Reducing critical effects of torsional vibrations induced by PDC bits during rock destruction by B. A. Ovezov, K. O. Shcherbakova, L. R. Kalendarova, P. A. Romanov, D. A. Kuznetsova

“…To do this, it is necessary to evaluate the drilling efficiency of the DE bit, therefore, this article has evaluated the parameter depending on the size of the bit, which will determine the critical value of the drilling efficiency parameter.…”
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Analisis Penerimaan Learning Management System Institut Teknologi Garut Menggunakan Technology Acceptance Model by Asri Mulyani, Dede Kurniadi, Mita Hidayani Putri

“…Because the Learning Management System at the Garut Institute of Technology has just been used, a study entitled Learning Management System Acceptance Analysis of the Garut Institute of Technology uses the Technology Acceptance Model Method, to determine the measurement of the influence between constructs as well as a benchmark for adapting user acceptance to the system used. …”
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Reimplante e revascularização da ponta do dedo: revisão de literatura e relato de caso de amputação da ponta do dedo mínimo na criança de um ano de idade Fingertip replantation and... by Chang Yung Chia, Tomaz Nassif

“…The literature on this topic is scant, and a study on the most practical and efficient method for fingertip replantation in small children is warranted.…”
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Methanol fixation and tagmentation of RNA/DNA hybrids directly enable single-cell transcriptome sequencing by Tao Xu, Yicong Xu, Ziyang An, Yiheng Li, Jiawen Yang, Weixing Zhang, Jin Xu

“…In situ reverse transcription followed by RNA/DNA hybrids transposition was performed to test the efficiency of these reactions. The transposed fragments were sequenced and investigated to determine transcriptome capture efficiency. …”
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