Exploring the functional role of POU5F1 using CRISPR RNP electroporation of zygote (CRISPR-EZ) in buffaloes by Meeti Punetha, Dharmendra Kumar, Suman Choudhary, Sheetal Saini, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, P. S. Yadav, T. K. Datta

“…In conclusion, direct electroporation of the CRISPR RNP component to the earlier stage of the zygote efficiently created mutations in the POU5F1 gene. We developed an easy and straightforward protocol for gene editing, which could serve as a valuable method for studying the functional genomics of the buffalo embryos. …”
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Transformation of the regulation of liability for tax offenses in the Russian Federation by E. L. Gulkova, E. A. Pshenichnova

“…The problems of tax administration in the sphere of taxation have been considered, as well as the prospects and directions offurther development and expansion of the applied methods of management of tax relations in the Russian Federation have been determined. …”
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Physicomathematical modeling of the formation features of fillet welds of bridge metal structures under submerged-arc welding by A. A. Mosin, V. A. Erofeev, M. A. Sholokhov

“…Comparison of the numerical analysis results with actual data on welding modes under the production of bridge metal structures shows that the existing fillet welding technologies have already reached their maximum efficiency rate. Further productivity gain is possible by forming oversized legs only with multiarc or multielectrode welding methods.…”
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FBMC vs. PAM and DMT for High-Speed Wireline Communication by Jeremy Cosson-Martin, Jhoan Salinas, Hossein Shakiba, Ali Sheikholeslami

“…Finally, we present a method to emulate wireline links while reducing the emulator complexity and simulation time by one to two orders of magnitude over conventional over-sampled techniques. …”
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Transition from the industrial clusters to the smart specialization: a case study by Yelena Petrenko, Elena Vechkinzova, Viktor Antonov

“…Kazakhstan faced the problem of falling industrial production and decrease in efficiency of former managerial methods of territorial development. …”
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Conception of Automated Control Traffic System Development in the Republic of Belarus by D. V. Kapsky, E. N. Kot

“…The proposed classification permits to structurize control methods and algorithms for every system and prepare a list of technical means for systems of various level. …”
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The Fisher Information Matrix as a Relevant Tool for Sensor Selection in Engine Health Monitoring by S. Borguet, O. Léonard

“…Engine health monitoring has been an area of intensive research for many years. Numerous methods have been developed with the goal of determining a faithful picture of the engine condition. …”
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Reducing time and computing costs in EC-Earth: an automatic load-balancing approach for coupled Earth system models by S. Palomas, M. C. Acosta, G. Utrera, E. Tourigny

“…</p> <p>The performance achieved depends on the coupling approach, as well as on the number of parallel resources and scalability properties of each component. Determining the appropriate number of resources to use for each component in coupled ESMs is crucial for efficient utilization of the high-performance computing (HPC) infrastructures used in climate modeling. …”
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Les ressources de l’environnement : au cœur de la construction des savoirs professionnels en situation de travail et de la protection de la santé by Céline Chatigny

“…The development of « operational resources » (useful and usable) is then the essence of strategies for developing efficient work methods (effective and safe). This is demonstrated by a comparative study in two employment sectors, where the working conditions are learning conditions, and where the margin of maneuver for implementing learning activities is different. …”
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Research of transformation changes in the noncommercial organizations’ management process in the conditions of military aggression by Olena Kanishchenko, Nataliia Chupryna, Hanna Dotsenko, Vira Zhuravel, Mariia Khudomaka

“…The stages of the transformation of the educational process in wartime conditions must be investigated and their relevance determined in one or another situation. Their number is determined under the influence of many factors, but the main one is ensuring personal safety and preserving the health of people and animals involved in the transformation process. …”
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Technical effectiveness of insecticides against oriental fruit mothand paech twigborerin peachorchards of the South of Ukraine by І. Yudytska, Yu. Klechkovskyi

“…Methods. Field methods. Technical effectiveness of insecticides was determined in peach orchards of Redhaven and Zolota Moskva cultivars. …”
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Performance Analysis in Production Systems with Uncertain Data: A Stochastic Data Envelopment Analysis Approach by Seyedeh Fatemeh Bagheri, Alireza Amirteimoori, Sohrab Kordrostami, Mansour Soufi

“…Previous methods for determining the projection points of inefficient DMUs have only focused on one objective and other features have been ignored. …”
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Mixed Land use Pattern in Deteriorated and Inefficient Neighborhoods of Region Eighteen of Tehran and its Role in Sustainable Neighborhood Regeneration by Mojtaba Roustaei, Ahmad Pour Ahmad, Nafiseh Marsousi, esmaeil ali akbari

“…The 18th Region of Tehran, as a part of such tissues of the country, is not exception to these problems and attention to efficient planning for their reconstruction can, while creating value, stimulate the process of tissue regeneration and solve problems within They will also meet part of the future development needs of Tehran and, as a result, prevent the growth of the outskirts of the city, whose infrastructure requires high costs. 2-Materials and Methods In the first step, information on the current status of the area and, deteriorated and inefficient urban areas is extracted from the basic maps of the detailed plan and land use of the area and field harvesting, by GIS software. …”
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Multirobot Coverage Path Planning Based on Deep Q-Network in Unknown Environment by Wenhao Li, Tao Zhao, Songyi Dian

“…Online decision-making relies on the sliding-view method and probability statistics to deal with the nonstandard size and unknown environments and the deduction method to improve the efficiency of coverage. …”
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Back-Analysis of Parameters of Jointed Surrounding Rock of Metro Station Based on Random Forest Algorithm Optimized by Cuckoo Search Algorithm by Xinping Guo, Annan Jiang, Xiang Liu

“…The efficiency and accuracy in determining mechanical parameters of joint of rock mass have significant influences on the safety of construction. …”
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A Highly Accurate Technique to Obtain Exact Solutions to Time-Fractional Quantum Mechanics Problems with Zero and Nonzero Trapping Potential by Muhammad Imran Liaqat, Adnan Khan, Md. Ashraful Alam, M. K. Pandit

“…The recurrence and absolute error of the four problems are analyzed to evaluate the efficiency and consistency of the presented method. …”
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Rapid detection and typing of Staphylococcus aureus based on nanopore Cas9-targeted sequencing by PAN Shufan, YANG Donglei, WANG Pengfei

“…Appropriate crRNAs were screened, the cleavage system was optimized, and both the cleavage reaction time and Cas9 RNP synthesis temperature were determined based on the results of Cas9 RNP cleavage efficiency testing. …”
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Detection of Streptococcus pneumoniae serotypes causing invasive and non-invasive infections using whole-genome sequencing by Mironov К.O., Gaponova I.I., Korchagin V.I., Mikhailova Yu.V., Shelenkov A.A., Chagaryan A.N., Ivanchik N.V., Platonov A.E., Kozlov R.S., Akimkin V.G.

“…The whole-genome sequences were obtained and strains serotypes were determined, while the efficiency of serotype determination by software was 97% (serotypes of 8 strains were determined ambiguously). …”
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Optimal segmentation and improved abundance estimation for superpixel-based Hyperspectral Unmixing by Qiang Guan, Tongyu Xu, Shuai Feng, Fenghua Yu, Kai Song

“…In the superpixel-based HU method, this study proposes a segmentation scale determination method to improve the accuracy of endmembers and fully constrained least squares based on distance strategy (D-FCLS) to improve the efficiency of abundance estimation. …”
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Effectiveness of The Umbrella Collaboration Versus Traditional Umbrella Reviews for Evidence Synthesis in Health Care: Protocol for a Validation Study by Beltran Carrillo, Marta Rubinos-Cuadrado, Jazmin Parellada-Martin, Alejandra Palacios-López, Beltran Carrillo-Rubinos, Fernando Canillas-Del Rey, Juan Jose Baztán-Cortes, Javier Gómez-Pavon

“…The implementation of new methodologies in evidence synthesis requires validation. This study will determine whether TU can match the accuracy and comprehensiveness of TURs while offering benefits in terms of efficiency and user accessibility. …”
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