Optimization and validation of a real-time PCR method for the simultaneous detection of Lactococcus garvieae and Streptococcus agalactiae in fish by Taona Zinyakasa, Farisai Chidzwondo, Tatenda Makawa, Sitokozile Sibanda, Exnevia Gomo, Tivapasi Musa, Elizabeth Gori

“…Currently, available pathogen diagnostic tools use biochemical and microbiological methods beleaguered by very low accuracy, reproducibility and specificity. …”
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Methodical approaches to the economic assessment of costs in the operation of cars with axial load of 27 tons at the section Kachkanar—Smychka by G. A. Granovskaya, A. I. Safonova, O. A. Suslov, N. S. Okhotnikov

“…Algorithms for calculating changes in the cost of fuel and energy costs for train traction and maintenance of the track infrastructure on the site during the operation of trains formed from cars with an axial load of 27 tons are given, as well as methods for determining the initial data for the calculation.Authors provide values of the coefficient reflecting the change in the impact of vertical and horizontal forces on the railway line when passing freight cars with an axial load of 27 tons compared to analogue cars, and the coefficient of change of the main specific resistance to motion separately for loaded and empty cars.Developed calculation algorithms and methods for obtaining baseline data allow an economic assessment of changes in infrastructure maintenance costs and fuel and energy resources for the operation of trains formed from cars with an axial load of 27 tons compared to those formed from cars with a load of 23.5 tons at the experimental section Kachkanar—Smychka.The cost change assessment carried out in 2017 shows a generally definite economic effect, while there is a reduction in costs associated with the consumption of electricity for train traction as a result of the operation of the estimated cars in the experimental section and an increase in the cost of maintaining the track superstructure and the roadbed, which is quite expected for the conditions of the organization of traffic with increased axial loads.…”
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An Analog Sensor Signal Processing Method Susceptible to Anthropogenic Noise Based on Improved Adaptive Singular Spectrum Analysis by Zhengyang Gao, Shuangchao Ge, Jie Li, Wentao Huang, Kaiqiang Feng, Chenming Zhang, Chunxing Zhang, Jiaxin Sun

“…ASSA addresses the challenge of determining the optimal number of decomposition layers, eliminating the parameter adjusting process and enhancing the measurement efficiency of sensor systems. …”
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Urine Catheter Sterilization from Adhered Biofilm Using Bimetallic Nanoparticles Synthesized Using the Plasma Reduction Method by Mohammed F. Al–Marjani, Fatima J. Hassan, Raghad S. Mohammed, Nisreen Kh. Abdalameer, Javed Iqbal

“…Objective: This study investigates the antibiofilm properties of MgO/ZnO and CuO/ZnO nanoparticles at concentrations of 10 and 40 µg/mL, respectively, against common bacterial pathogens, specifically Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus, and their potential to sterilize urinary catheters. Methods: A 96-well microtiter plate was used to determine sublethal dosage levels (Sub-MIC) and minimum inhibitory concentrations (MIC) to evaluate antibacterial activity. …”
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Research on automatic identification method for pipeline girth weld defects based on X-ray images and sparse representation by Shaohui JIA, Yaping LI, Weixin GAO, Yunchao PENG, Xinjian ZHANG, Yuxia WANG

“…Conclusion The proposed defect identification method demonstrates its capability to greatly improve the detection quality and efficiency of potential safety hazards in pipeline girth welds, underscoring its promising prospects in industrial applications.…”
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A Method for the Isolation of Thymoquinone from Black Caraway Seed Oil (<i>Nigella sativa</i> L.) by A. K. Whaley, Y. V. Burtseva, E. V. Kuldyrkaeva, A. S. Novosad, N. L. Babak, E. V. Zhokhova, A. O. Whaley, M. Yu. Goncharov, I. I. Terninko, G. P. Yakovlev

“…The proposed express and efficient method for the isolation of thymoquinone through steam distillation provides a yield of the target component of 0.26 %. …”
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Post-evaluation model for 500 kV substation engineering projects using the fuzzy comprehensive evaluation method by Jianquan Chen, Yi Zhuang, Shaoyong Chen, Zhongtian Zhang, Yu Zhang

“…First, the analytic hierarchy process is employed to determine the weights of each evaluation indicator, taking into account factors such as planning effectiveness, preliminary achievements, bid management efficiency, construction performance, project outcomes and benefits, social and environmental impacts, and project sustainability. …”
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Development of a method for calculation of demand for medical personnel in the healthcare of the Russian Federation, using a mathematical model by V. V. Omelyanovsky, T. V. Semenova, D. V. Lukyantseva, D. V. Fedyaev, E. A. Shalaeva

“…The study is aimed to determine and test the approaches to the development of a method for calculation of the demand for medical personnel in the healthcare in the Russian Federation, using a mathematical model of human resource for healthcare HRH balancing.Materials and methods. …”
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A method for modifying carbonaceous materials with manganese carbonate to module the formation of singlet oxygen via activation with Peroxymonosulfate by Camila Mosquera-Olano, Carolina Quimbaya, Santiago López-Pérez, Elkin Castellón-Castrillón, Sandra Navarro, John Rojas, Jorge Acosta, Ricardo A. Torres-Palma, Yenny P. Ávila-Torres

“…This method is significant as follows: • Low-cost carbonaceous materials activated with PMS demonstrated significant efficiency in degrading organic molecules, utilizing singlet oxygen as the primary oxidant. • Theoretical computational approaches investigate the interaction between material and molecule. • The role of carbonate ions in influencing singlet oxygen production in materials derived from residual biomass was examined.…”
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Synergetic effects of multiple junction and surface hydroxyl in Cu/CuO/Cu2O/TiO2 heterostructures towards highly efficient photocatalysts for hydrogen generation by Riki Subagyo, Garcelina Rizky Anindika, Afif Akmal Aufkani, Lei Zhang, Hosta Ardhyananta, R.Y. Perry Burhan, Zjahra Vianita Nugraheni, Syafsir Akhlus, Hasliza Bahruji, Didik Prasetyoko, Diana Vanda Wellia, Atthar Luqman Ivansyah, Arramel, Yuly Kusumawati

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Optimization of texture design parameters for reduction of friction coefficient based on design of experiments and the modified acceleration gradient method by Hideto ODA, Takahiko KURAHASHI, Demófilo MALDONADO CORTÉS, Laura PEÑA-PARÀS

“…In this study, we investigate a method of determining texture design parameters based on design of experiments and the modified acceleration gradient method. …”
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Multi-UAV Cooperative Air Combat Target Assignment Method Based on VNS-IBPSO in Complex Dynamic Environment by Yiyuan Li, Weiyi Chen, Shukan Liu, Guang Yang, Fan He

“…Then, by incorporating the analytic hierarchy process and entropy method, we determine the normalized threat value of the target considering both objective situation data characteristics and decision-maker preferences. …”
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Numerical and analytical investigation of Jeffrey nanofluid convective flow in magnetic field by FEM and AGM by Mohammad ِDehghan Afifi, Ali Jahangiri, Mohammad Ameri

“…Solving and developing non-linear differential equations is done using finite element method (FEM) and Akbari-Ganji method (AGM). The novelty of this research lies in its application of these methods to investigate the effects of varying physical parameters on Jeffrey nanofluid flow, thereby filling a significant gap in the field of magnetohydrodynamics (MHD). …”
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MODEL OF DISTRIBUTION OF THE BUDGET OF THE PORTFOLIO OF IT PROJECTS TAKING IN-TO ACCOUNT THEIR PRIORITY by Anita V. Sotnikova

“…The essence of a method of analytical net-works consists in paired comparison of key indicators of efficiency concerning the purpose of realization of a portfolio and IT projects which are a part of a portfolio. …”
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Core Competency Assessment Model for Entry-Level Air Traffic Controllers Based on International Civil Aviation Organization Document 10056 by Yi Hu, Hanyang Shen, Bing Wang, Jichuan Teng, Chenglong Guo, Yanjun Wang

“…With the increasing air traffic flow, the workload of air traffic controllers is also growing, and their proficiency directly impacts civil aviation safety and efficiency. To address the lack of clear training objectives and inconsistent evaluation methods in the initial controller training at the Southwest Air Traffic Management Bureau, this study aimed to develop and validate a core competency model for initial air traffic controllers. …”
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Efficiency in stress echo service delivery without compromising on diagnostic accuracy: quality improvement project on the need to advise cessation of rate-controlling medication b... by Emil Tom John, Laya Hariharan, Muhammad (Syed Zohaib) Amjad, Attila Kardos

“…Objective: To assess the efficacy of dobutamine SE (DSE) in a cohort of patients where discontinuation of RCMx was not required, we conducted a 1-year review of outcomes in individuals evaluated for suspected coronary artery disease (CAD) Method: 227 consecutive patients underwent DSE between January 2022 and January 2023 in a single centre. …”
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The efficiency of metabolic impact of S-adenosylmethionine and meldonium on parameters of lipid profile and insulin resistance during comorbid course of nonalcocholic steatohepatit... by O. S. Khukhlina, A. A. Antoniv, O. S. Voievidka, O. B. Kuzminska

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Identification of truncation frequencies for BIW structures based on modal contribution analysis by Feitan Peng, Huabin Li, Fuwu Yan, Junyi Ye

“…To address the trade-off between modeling efficiency and accuracy in low- to mid-frequency dynamic analysis of Body-in-White (BIW) structures, this study proposes a frequency truncation method based on the modal contribution at points of minimum dynamic stiffness. …”
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The problem of creating and developing an integrated approach to standardization process of automated testing of turboexpanders by V. M. Mamedov, I. A. Arkharov

“…In accordance with the general classification of product testing, 9 types of research have been identified that are of scientific and practical interest in the experimental determination of the most relevant parameters of turboexpanders: isentropic efficiency, stability of the rotor and impellers, strength of impellers, vibration and noise characteristics.Conclusion. …”
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Spike Stall Precursor Detection in a Single-Stage Axial Compressor: A Data-Driven Dynamic Modeling Approach by Anish Thapa, Jichao Li, Marco P. Schoen

“…Operational safety and fuel efficiency are critical, yet often conflicting, objectives in modern civil get engine designs. …”
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