Optimization and validation of a real-time PCR method for the simultaneous detection of Lactococcus garvieae and Streptococcus agalactiae in fish by Taona Zinyakasa, Farisai Chidzwondo, Tatenda Makawa, Sitokozile Sibanda, Exnevia Gomo, Tivapasi Musa, Elizabeth Gori

“…Currently, available pathogen diagnostic tools use biochemical and microbiological methods beleaguered by very low accuracy, reproducibility and specificity. …”
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Methodical approaches to the economic assessment of costs in the operation of cars with axial load of 27 tons at the section Kachkanar—Smychka by G. A. Granovskaya, A. I. Safonova, O. A. Suslov, N. S. Okhotnikov

“…Algorithms for calculating changes in the cost of fuel and energy costs for train traction and maintenance of the track infrastructure on the site during the operation of trains formed from cars with an axial load of 27 tons are given, as well as methods for determining the initial data for the calculation.Authors provide values of the coefficient reflecting the change in the impact of vertical and horizontal forces on the railway line when passing freight cars with an axial load of 27 tons compared to analogue cars, and the coefficient of change of the main specific resistance to motion separately for loaded and empty cars.Developed calculation algorithms and methods for obtaining baseline data allow an economic assessment of changes in infrastructure maintenance costs and fuel and energy resources for the operation of trains formed from cars with an axial load of 27 tons compared to those formed from cars with a load of 23.5 tons at the experimental section Kachkanar—Smychka.The cost change assessment carried out in 2017 shows a generally definite economic effect, while there is a reduction in costs associated with the consumption of electricity for train traction as a result of the operation of the estimated cars in the experimental section and an increase in the cost of maintaining the track superstructure and the roadbed, which is quite expected for the conditions of the organization of traffic with increased axial loads.…”
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An Analog Sensor Signal Processing Method Susceptible to Anthropogenic Noise Based on Improved Adaptive Singular Spectrum Analysis by Zhengyang Gao, Shuangchao Ge, Jie Li, Wentao Huang, Kaiqiang Feng, Chenming Zhang, Chunxing Zhang, Jiaxin Sun

“…ASSA addresses the challenge of determining the optimal number of decomposition layers, eliminating the parameter adjusting process and enhancing the measurement efficiency of sensor systems. …”
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Urine Catheter Sterilization from Adhered Biofilm Using Bimetallic Nanoparticles Synthesized Using the Plasma Reduction Method by Mohammed F. Al–Marjani, Fatima J. Hassan, Raghad S. Mohammed, Nisreen Kh. Abdalameer, Javed Iqbal

“…Objective: This study investigates the antibiofilm properties of MgO/ZnO and CuO/ZnO nanoparticles at concentrations of 10 and 40 µg/mL, respectively, against common bacterial pathogens, specifically Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus, and their potential to sterilize urinary catheters. Methods: A 96-well microtiter plate was used to determine sublethal dosage levels (Sub-MIC) and minimum inhibitory concentrations (MIC) to evaluate antibacterial activity. …”
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Some Philosophical Problems of Studying Complex Systems in the Universe by A. V. Nesteruk, A. V. Soldatov

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Research on automatic identification method for pipeline girth weld defects based on X-ray images and sparse representation by Shaohui JIA, Yaping LI, Weixin GAO, Yunchao PENG, Xinjian ZHANG, Yuxia WANG

“…Conclusion The proposed defect identification method demonstrates its capability to greatly improve the detection quality and efficiency of potential safety hazards in pipeline girth welds, underscoring its promising prospects in industrial applications.…”
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Effects of Different Rearing Methods on the Intestinal Morphology, Intestinal Metabolites, and Gut Microbiota of Lueyang Black-Bone Chickens by Shuang Zeng, Linqing Shao, Mingming Zhao, Ling Wang, Jia Cheng, Tao Zhang, Hongzhao Lu

“…The intestinal morphology, intestinal metabolites, and gut microbiota are critical determinants of nutrient utilization efficiency and immune health in poultry. …”
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A Method for the Isolation of Thymoquinone from Black Caraway Seed Oil (<i>Nigella sativa</i> L.) by A. K. Whaley, Y. V. Burtseva, E. V. Kuldyrkaeva, A. S. Novosad, N. L. Babak, E. V. Zhokhova, A. O. Whaley, M. Yu. Goncharov, I. I. Terninko, G. P. Yakovlev

“…The proposed express and efficient method for the isolation of thymoquinone through steam distillation provides a yield of the target component of 0.26 %. …”
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Post-evaluation model for 500 kV substation engineering projects using the fuzzy comprehensive evaluation method by Jianquan Chen, Yi Zhuang, Shaoyong Chen, Zhongtian Zhang, Yu Zhang

“…First, the analytic hierarchy process is employed to determine the weights of each evaluation indicator, taking into account factors such as planning effectiveness, preliminary achievements, bid management efficiency, construction performance, project outcomes and benefits, social and environmental impacts, and project sustainability. …”
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Development of a method for calculation of demand for medical personnel in the healthcare of the Russian Federation, using a mathematical model by V. V. Omelyanovsky, T. V. Semenova, D. V. Lukyantseva, D. V. Fedyaev, E. A. Shalaeva

“…The study is aimed to determine and test the approaches to the development of a method for calculation of the demand for medical personnel in the healthcare in the Russian Federation, using a mathematical model of human resource for healthcare HRH balancing.Materials and methods. …”
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A method for modifying carbonaceous materials with manganese carbonate to module the formation of singlet oxygen via activation with Peroxymonosulfate by Camila Mosquera-Olano, Carolina Quimbaya, Santiago López-Pérez, Elkin Castellón-Castrillón, Sandra Navarro, John Rojas, Jorge Acosta, Ricardo A. Torres-Palma, Yenny P. Ávila-Torres

“…This method is significant as follows: • Low-cost carbonaceous materials activated with PMS demonstrated significant efficiency in degrading organic molecules, utilizing singlet oxygen as the primary oxidant. • Theoretical computational approaches investigate the interaction between material and molecule. • The role of carbonate ions in influencing singlet oxygen production in materials derived from residual biomass was examined.…”
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Comparative Study of Fish Diversity in Wanquan River Based on Environmental Technology and Traditional Fishing Gear Survey Methods by Jun LEI, Yuanyuan SU, Lianzheng YIN, Ruohan ZENG, Ping LI, Yongqiang QIN, Xingwei CAI, Xiaoping DIAO

“…Environmental DNA (eDNA) technology involves analyzing biological communities by enriching DNA retained in the environment and using high-throughput sequencing technology. This method offers several advantages, including minimal environmental disturbance, time and effort efficiency, and allowing rapid monitoring of fish diversity and spatial distribution. eDNA technology has been widely used in biodiversity monitoring, population distribution, biomass assessment, and feeding analysis. …”
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Photocatalytic Degradation of Acid Black 210 dye Using Fixed Bed Reactor Containing Zinc Oxide Nanoparticles: Effects and Box–Behnken Optimization by Zahraa A. Najm, Mohammed A. Atiya, Ahmed K. Hassan

“…The optimized parameters for the fixed-bed system were with a removal efficiency of 69% determined as 2.25 mL/min, 1.21 cm, 30 mg/L, and 15 W/m2 for flow rate, ZnO-NPs height, AB210 concentration, and UV intensity respectively. …”
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Acoustic emission localization model of concrete material based on convolutional neural network by DENG Yongdong, ZHOU Jingren, LU Xiang, CHEN Jiangkang

“…Therefore, the selection of the initial value of iteration is particularly important for the traditional iterative localization method. Moreover, the traditional iterative localization methods are greatly affected by the number of sensors and environmental noise, resulting in unstable localization efficiency. …”
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Optimization of texture design parameters for reduction of friction coefficient based on design of experiments and the modified acceleration gradient method by Hideto ODA, Takahiko KURAHASHI, Demófilo MALDONADO CORTÉS, Laura PEÑA-PARÀS

“…In this study, we investigate a method of determining texture design parameters based on design of experiments and the modified acceleration gradient method. …”
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Computational modelling of head-related transfer function by Andrzej B. DOBRUCKI, Przemysław PLASKOTA

“…Measuring the Head-Related Transfer Function (HRTF) is an efficient method that takes into consideration the influence of the human body on the sound spectrum. …”
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Multi-UAV Cooperative Air Combat Target Assignment Method Based on VNS-IBPSO in Complex Dynamic Environment by Yiyuan Li, Weiyi Chen, Shukan Liu, Guang Yang, Fan He

“…Then, by incorporating the analytic hierarchy process and entropy method, we determine the normalized threat value of the target considering both objective situation data characteristics and decision-maker preferences. …”
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Multiliteracies meet new methods: The case for digital writing in English education case study in G-7 countries. by Xiaoying Hu

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Multi-Parameter Structural Optimization of Shale-Hydrocarbon-Dissolvable Ball Seat Slips Based on Safety and Performance Assessment Methods by Shuang Jing, Anle Mu, Zhen Chen, Xiaoyang Ying, Nengpeng Chen, Qingjie Ran

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Determinación del tiempo de deshumidificación en un almacén soterrado en las condiciones climáticas y geohidrológicas de Cuba. // Determination of dehumidifier time in underground... by Ma. D. Andrade Gregori, R. Hernández Rubio, M. Piedra Díaz

“…In the<br />proposed method calculation, the efficiency is used as fundamental economic indicator of the dehumidification process.…”
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