A sensitive sample preparation pipeline for adventitious virus detection using Oxford Nanopore sequencing by Emmanuel K. Tsinda, Charles A. Swofford, James P.B. Strutt, José Sangerman, Raeuf Roushangar, Alexander Li, Lara M. Gomez, Aidan N. Bousquet, Caleb Neufeld, Jacqueline M. Wolfrum, Jongyoon Han, Rohan B.H. Williams, Anthony J. Sinskey, Paul W. Barone, Stacy L. Springs

“…To address this, we have developed a sensitive sample preparation workflow using concentration, nuclease treatment, and agnostic PCR methods to eliminate background signals and amplify viral contaminant reads, leading to a 3-log improvement in the limit of detection that is comparable to or better than short-read sequencing approaches. …”
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Evaluating the Efficacy of a Polymerase Chain Reaction Assay for Pneumocystis carinii Detection in Respiratory Samples by Sonu Kumari Agarwal, Harendra Kumar Thakur, Bansidhar Tarai

“…In this study, sputum and bronchoalveolar lavage (BAL) samples were proceeded for both staining and PCR for multi-copy mtLSU gene and single-copy dihydropteroate synthase polymorphisms (DHPS) fas gene of P. jirovecii. …”
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Enzyme Inhibition-Mediated Distance-Based Paper Biosensor for Organophosphate Pesticide Detection in Food Samples by Yulin Liu, Longzhan Dong, Qiognzheng Hu, Jingbo Chen, Mashooq Khan

“…This highlights the need to monitor OPs in food and environmental samples. This study introduces an enzyme inhibition-mediated distance-based paper (EIDP) biosensor designed for naked-eye visual detection of OPs in food samples. …”
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Detection and Whole-Genome Analysis of tigecycline resistant Escherichia coli in poultry and meat samples in Türkiye by Cemil Kürekci, Xiaoyu Lu, Seyda Sahin, Büşra Gülay Celil, Yeşim Soyer, Filiz Yeniköylü, Zhiqiang Wang, Ruichao Li

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Association of Molecular Detections of Microsporidia in Stool Samples with Clinical and Immunological Parameters in Ghanaian HIV Patients by Hagen Frickmann, Fred Stephen Sarfo, Betty Roberta Norman, Albert Dompreh, Shadrack Osei Asibey, Richard Boateng, Edmund Osei Kuffour, Konstantin Tanida, Veronica Di Cristanziano, Torsten Feldt, Kirsten Alexandra Eberhardt

“…Although the etiological relevance of the detection of microsporidia in human stool samples remains uncertain, the immunological status of patients has been posited as an important determinant of potential clinical impact of these parasites. …”
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Detection of human herpesvirus 7 in conjunctival samples collected from individuals recovering from conjunctivitis by Andrew T.E. Hartwick, Christina E. Morettin, Jennifer S. Harthan, Meredith Whiteside, Ellen Shorter, Spencer D. Johnson, Mary K. Migneco, Christian K. Olson, Julia B. Huecker, Tammy Than, Mae O. Gordon

“…None of the samples tested positive for HSV-1, HSV-2, HHV-6A or HHV-6B.ConclusionThis data provides further evidence that Human Herpesvirus 7 can be present in the eye, as HHV-7 was detected in a subset of conjunctival samples obtained from individuals recovering from non-adenoviral conjunctivitis. …”
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Molecular detection of clarithromycin-resistant Helicobacter pylori in stool samples using real-time PCR by Menna allah Mohamed, Rania Abdel Halim, Névine Kassem, Eman kamel, sally saber, Engy Elsayed, samar rashad

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In-House Immunoglobulin Y-Based Immunoassay for Detecting Benzo[a]pyrene in Grilled Pork Samples by Peerapong Jeeno, Sumed Yadoung, Marninphan Thongkham, Pichamon Yana, Udomsap Jaitham, Sakaewan Ounjaijean, Zhen-Lin Xu, Korawan Sringarm, Surat Hongsibsong

“…The objective of this study is to create an immunoassay for the detection of benzo[a]pyrene utilizing immunoglobulin Y antibodies. …”
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Detecting Long-Term Spatiotemporal Dynamics of Urban Green Spaces with Training Sample Migration Method by Mengyao Wang, Pan Li, Chunyu Wang, Wei Chen, Zhongen Niu, Na Zeng, Xingxing Han, Xinchao Sun

“…UGSs coverage in sample areas increased from 5% to 38%, resulting in more reliable trend detection. …”
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Development of A Real-Time PCR Assay for Plasmodiophora brassicae and Its Detection in Soil Samples by Jin-ping LI, Yan LI, Yan-xia SHI, Xue-wen XIE, Chai A-li, Bao-ju LI

“…Naturally and artificially infested soil samples containing different concentrations of P. brassicae were detected. …”
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Human papillomavirus DNA detection in cervical samples from women of reproductive age in Mogadishu, Somalia by Bashiru Garba, Maryan A Sh Nur, Maryam Adawe, Mohamed H Adam, Jamal H Mohamoud, Hassan A Dahie, Mohamed M Ahmed, Abdirahman K Mohamud, Sadio A Mohamed, Fartun AH Orey, Jihaan Hassan, Hodo A Asowe, Najib I Dirie

“…Results: Overall, 31.7% (60/189) of cervical samples were positive for HPV DNA. Out of this, 19.6% were high risk-HPV (hrHPV), 13.8% were HPV16, and 5.3% were HPV18. …”
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Detection and Profiling of Antibiotic Resistance among Culturable Bacterial Isolates in Vended Food and Soil Samples by Susan W. Muriuki, Johnstone O. Neondo, Nancy L. M. Budambula

“…This study aimed at detecting and profiling antibiotic resistance of bacterial contaminants in vended food and the environment. …”
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How, What, and Where You Sample Environmental DNA Affects Diversity Estimates and Species Detection by Anish Kirtane, Leif Howard, Caitlin E. Beaver, Margaret E. Hunter, Gordon Luikart, Kristy Deiner

“…In comparison, the beta diversity of plant eDNA was less impacted by the sampling method. We found no clear difference in detection for the invasive species targets based on the eDNA sampling method. …”
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Methods for the detection of tumor-specific single nucleotide somatic mutations in plasma cDNA samples by L. M. Dyakov, O. M. Krivtsova, P. A. Khesina, I. F. Kustova, N. A. Dyakova, N. S. Muge, N. E. Kudashkin, Yu. I. Patyutko, N. L. Lazarevich

“…We used normal and tumor tissue, as well as blood plasma samples from patients with hepatocellular carcinoma, and various methods for detecting single-nucleotide somatic mutations: real-time polymerase chain reaction (PCR) with intercalating dye or with TaqMan probes, droplet digital PCR and high-throughput sequencing of target amplicons.Results. …”
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A novel electrochemical immunosensor based on biomaterials for detecting carcinoembryonic antigen biomarker in serum samples by Sajedeh Sobhanparast, Payam Shahbazi-Derakhshi, Jafar Soleymani, Amir Amiri-Sadeghan, Alireza Herischi, Nader Chaparzadeh, Younes Aftabi

“…The results highlight the potential clinical applicability of this electrochemical immunosensor for detecting CEA levels in human serum samples.…”
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Iron pyrite nanocrystals as a sustainable nanozyme for colorimetric detection of ceftriaxone sodium in environmental samples by Seyyed Morteza Seyyed Alavi, Mohammad Amjadi, Jamshid L. Manzoori

“…Applicability of these sensors was studied for analysis of various water samples, demonstrating their great potential for real analytical applications.…”
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Detection Of Shiga Toxin Genes Presence in Escherichia coli Isolated from Chicken Fecal Samples by Sura J. Mohammed, Ali Iedani

“…The results of this technique indicated that 84 (77%) of them were Escherichia coli found in the examined samples. All isolates were undergone a polymerase chain reaction to detect Shiga-toxins genes (stx1, stx2). …”
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Comparing sputum, nasopharyngeal swabs, and combined samples for respiratory bacterial detection using multiplex PCR by Keun Ju Kim, Seung Gyu Yun, Myung-Hyun Nam, Chang Kyu Lee, Yunjung Cho

“…Multiplex quantitative PCR (qPCR) is commonly used for pathogen detection in respiratory samples. However, the optimal specimen selection for detecting bacterial pathogens is not well-explored. …”
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Spectrophotometric and cloud point extraction methods to detect Quercetin Dihydrate in supplement formulations and urine samples by Sadeem Subhi Abed, Mayasa Mansour Mohammed

“… The accurate detection and quantification of quercetin dihydrate (QRC) are vital for quality control, pharmacokinetic studies, and bioavailability assessments in pharmaceutical and biological samples. …”
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Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection by Fu-chao Chen, Lin-hai Wang, Jun Guo, Xiao-ya Shi, Bao-xia Fang

“…A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. …”
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