Diagnostic utility of auramine O smear microscopy for the detection of non-tuberculous mycobacteria vs Mycobacterium tuberculosis complex in adult clinical samples: a 3-year retrospective cohort study (2019-2021) by Dr Leong Tung Ong, Dr Azwani Abdullah, Dr Thian Chee Loh, Dr Chandramathi Samudi Raju, Ms Jennifer Chong, Dr Nadia Atiya

“…These findings highlight the need for more accurate and reliable diagnostic techniques for detecting NTM and MTBC directly from clinical samples.…”
Get full text

A comparative study evaluating three line immunoassays available for serodiagnosis of equine Lyme borreliosis: Detection of Borrelia burgdorferi sensu lato-specific antibodies in serum samples of vaccinated and non-vaccinated horses. by Cornelia V Broeckl, Stephanie Hiereth, Reinhard K Straubinger

“…In this study, three LIAs available for detection of antibodies in equine serum samples were evaluated and compared. …”
Get full text

Macroepidemiological trends of Influenza A virus detection through reverse transcription real-time polymerase chain reaction (RT-rtPCR) in porcine samples in the United States over the last 20 years by Daniel C. A. Moraes, Guilherme A. Cezar, Edison S. Magalhães, Rafael R. Nicolino, Kinath Rupasinghe, Srijita Chandra, Gustavo S. Silva, Marcelo N. Almeida, Bret Crim, Eric R. Burrough, Phillip C. Gauger, Darin Madson, Joseph Thomas, Michael A. Zeller, Rodger Main, Mary Thurn, Paulo Lages, Cezar A. Corzo, Mattew Sturos, Hemant Naikare, Rob McGaughey, Franco Matias Ferreyra, Jamie Retallick, Jordan Gebhardt, Sara McReynolds, Jon Greseth, Darren Kersey, Travis Clement, Angela Pillatzki, Jane Christopher-Hennings, Beth S. Thompson, Melanie Prarat, Dennis Summers, Craig Bowen, Joseph Boyle, Kenitra Hendrix, James Lyons, Kelli Werling, Andreia G. Arruda, Mark Schwartz, Mark Schwartz, Paul Yeske, Deborah Murray, Brigitte Mason, Peter Schneider, Samuel Copeland, Luc Dufresne, Daniel Boykin, Corrine Fruge, William Hollis, Rebecca C. Robbins, Thomas Petznick, Kurt Kuecker, Lauren Glowzenski, Megan Niederwerder, Daniel C. L. Linhares, Giovani Trevisan

“…Of the total of 118,490 samples tested for IAV subtyping using RT-rtPCR, the most frequently detected subtypes were H1N1 (33.1%), H3N2 (25.5%), H1N2 (24.3%), H3N1 (0.2%), mixed subtypes (5.4%), and partial subtype detection (11.5%). …”
Get full text

SARFA-Net: Shape-Aware Label Assignment and Refined Feature Alignment for Arbitrary-Oriented Object Detection in Remote Sensing Images by Yan Dong, Minghong Wei, Guangshuai Gao, Chunlei Li, Zhoufeng Liu

Subjects: “…Arbitrary-oriented object detection (AOOD)…”
Get full text

Screening for Sexually Transmitted Infection Pathogens in Semen Samples by RW Peeling, J Embree

“…Although viral STI pathogens and Treponema pallidum -- the causative agent of syphilis -- may be detected in semen, their presence may be more sensitively detected through antibody testing of the donor. …”
Get full text

Sources of variability in metabolite measurements from urinary samples. by Qian Xiao, Steven C Moore, Simina M Boca, Charles E Matthews, Nathaniel Rothman, Rachael Z Stolzenberg-Solomon, Rashmi Sinha, Amanda J Cross, Joshua N Sampson

“…<h4>Conclusions</h4>The use of metabolomics in urine samples from epidemiological studies would require large sample sizes to detect associations with moderate effect sizes.…”
Get full text

Intraspecies associations from strain-rich metagenome samples by Evan B. Qu, Jacob S. Baker, Laura Markey, Veda Khadka, Chris Mancuso, A. Delphine Tripp, Tami D. Lieberman

“…Summary: Genetically distinct strains of a species can vary widely in phenotype, reducing the utility of species-resolved microbiome measurements for detecting associations with health or disease. While metagenomics theoretically provides information on all strains in a sample, current strain-resolved analysis methods face a tradeoff: de novo genotyping approaches can detect novel strains but struggle when applied to strain-rich or low-coverage samples, while reference database methods work robustly across sample types but are insensitive to novel diversity. …”
Get full text

Characterization of oral swab samples for diagnosis of pulmonary tuberculosis. by Rachel C Wood, Alfred Andama, Gleda Hermansky, Stephen Burkot, Lucy Asege, Mukwatamundu Job, David Katumba, Martha Nakaye, Sandra Z Mwebe, Jerry Mulondo, Christine M Bachman, Kevin P Nichols, Anne-Laure M Le Ny, Corrie Ortega, Rita N Olson, Kris M Weigel, Alaina M Olson, Damian Madan, David Bell, Adithya Cattamanchi, William Worodria, Fred C Semitala, Akos Somoskovi, Gerard A Cangelosi, Kyle J Minch

“…A qPCR targeting conserved bacterial ribosomal rRNA gene (rDNA) sequences was used to quantify bacterial biomass in samples. There was no detectable reduction in total bacterial rDNA signal over the course of 10 rapidly repeated tongue samplings, indicating that swabs collect only a small portion of the biomass available for testing. …”
Get full text

Improved sample preparation using bead mill homogenization by Madison E. Walbrun, Aamna Aijaz, Gabriella Ryan, John D. Bunting, Zachary P. Morehouse, Rodney J. Nash

“…Proper sample preparation is the key to having successful data analysis downstream. …”
Get full text

The structure of affective fluctuations in a non-clinical sample by N. N. Osipova, L. M. Bardenstein, G. A. Aleshkina, E. V. Dmitrieva

“…Upon a clinical and psychopathological examination according to ICD-10 criteria, class V, mental and behavioral disorders (F00-F99), including affective pathology, were not detected. According to the findings of the HCL-32 screening technique, 61.2% (n = 79) of respondents had a cumulative score above the threshold. …”
Get full text

Assessment of Sr-90 in water samples: precision and accuracy by Marcelo Bessa Nisti, Cátia H.R. Saueia, Bruna Castilho, Barbara P. Mazzilli

“…The parameters Minimum Detectable Activity (MDA) and Figure Of Merit (FOM) were determined for each method, the precision and accuracy were checked using 90Sr standard solutions.…”
Get full text

On the pursuit of reproducibility: the importance of large sample sizes in psychoimmunology by Manivel Rengasamy, Daniel Moriarity, Rebecca Price

“…We further assessed how statistical errors varied as related to analytic decisions (e.g., number of covariates) and characteristics related to study design (e.g., relationships within subgroups of patients). Only large samples (e.g., n = 1000 to n = 10,000) were sufficiently powered to detect PIM-depression associations and minimized overestimation of effect sizes (e.g., effect size inflation), and greater sample sizes were required as more covariates were included in analytic models. …”
Get full text

Ensuring representative sample volume predictions in microplastic monitoring by Richard K. Cross, Sarah L. Roberts, Monika D. Jürgens, Andrew C. Johnson, Craig W. Davis, Todd Gouin

“…We have developed the Representative Sample Volume Predictor (RSVP) tool, which standardises statistical prediction of sufficient sample volumes, to ensure microplastics are detected with a given level of confidence. …”
Get full text

Discussion on the Analysis Method of Iodide in Natural Water Samples by Xinyi SONG, Chunxue XU, Ziyi AN, Hongbin SUN, Zongding CHEN, Wei LIU, Yuqi ZHENG

“…BACKGROUNDAs the main source of iodine intake, water is of practical significance to detect the content of iodide accurately. At present, the common methods for the analysis of iodide in water samples include ion chromatography, gas chromatography, colorimetry, spectrophotometry. …”
Get full text

Case Report: Anomalous drainage vein sampling for diagnosing aldosterone-producing lesions undetectable by segmental adrenal venous sampling in a two-case series by Hiromitsu Tannai, Sota Oguro, Hiroki Kamada, Yuta Tezuka, Yoshikiyo Ono, Kei Omata, Kei Takase

“…Compared with conventional AVS, segmental AVS with additional sampling in adrenal tributaries can detect aldosterone-producing adenomas (APAs) with higher sensitivity. …”
Get full text

Performance Evaluation of a Fully Automated Molecular Diagnostic System for Multiplex Detection of SARS-CoV-2, Influenza A/B Viruses, and Respiratory Syncytial Virus by James G. Komu, Dulamjav Jamsransuren, Sachiko Matsuda, Haruko Ogawa, Yohei Takeda

“…Using saliva samples, the viral titer detection limits of geneLEAD/VIASURE and manual referent singleplex RT-qPCR assays were compared. …”
Get full text

Initial evaluation of a state-wide multi-method survey to estimate koala occupancy and abundance by Graeme R. Gillespie, Nicole Gallahar, Allen P. McIlwee, Benjamin Douglas Hope, Elliot B. Webb, Matthew Clements, Veronica F. Gama, Madison Casley, Tim S. Jessop

Subjects: Get full text

A Laser-Based SLAM Algorithm of the Unmanned Surface Vehicle for Accurate Localization and Mapping in an Inland Waterway Scenario by Yang Wang, Chao Liu, Jiahe Liu, Jinzhe Wang, Jianbin Liu, Kai Zheng, Rencheng Zheng

Subjects: Get full text

High-Performance Anion Exchange Chromatography Electrochemical Determination of Uric Acid as a Contamination Marker by Kevin C. Honeychurch

Subjects: Get full text

Analytical performance evaluation of a multiplex real-time RT-PCR kit for simultaneous detection of SARS-CoV-2, influenza A/B, and RSV by Chi-Sheng Tai, Ming-Jr Jian, Tai-Han Lin, Hsing-Yi Chung, Chih-Kai Chang, Cherng-Lih Perng, Po-Shiuan Hsieh, Hung-Sheng Shang

“…RNA standards were serially diluted and tested with the LabTurbo kit to determine the limit of detection (LOD). This cross-sectional study involved the analysis of 350 nasopharyngeal swab samples, which included 250 positive cases (50 cases each of influenza A, influenza B, and RSV, along with 100 cases of SARS-CoV-2) and 100 negative cases. …”
Get full text