In Vitro Structure–Activity Relationship Stability Study of Antisense Oligonucleotide Therapeutics Using Biological Matrices and Nucleases by Jelena Lovrić, Jingjing Yan, XueQing Li, Tony Karlsborn, Mattias Bood, Anders Dahlén, Constanze Hilgendorf, Deepak Kumar Bhatt

“…Stability studies of ASOs with varying phosphorothioate/phosphodiester (PS/PO) content were conducted using nucleolytic matrices (snake venom 3′‐exonuclease phosphodiesterase I [PDEI], mouse serum, and mouse liver homogenate) and analyzed by gel electrophoresis and liquid chromatography with ultraviolet and mass spectrometry detection (LC‐UV/MS). Both sequence composition and backbone chemistry play important roles in influencing the stability of ASOs. …”
Get full text

Translocation of probiotics via gut–lung axis enhanced pulmonary immunity of weaned piglets exposed to low concentrations of ammonia by Jiajun Yang, Jing Wang, Peng Shang, Zongliang Liu, Bo Zhang, Dongsheng Yang, Hao Zhang

“…More Lactobacillus reuteri and anaerobic probiotic bacteria were detected in the lungs. Conclusion: The colonization of Lactobacillus salivarius and Lactobacillus reuteri in the membrane of the ileum optimized the ileal microbial composition, enrolled other probiotic bacteria translating to the lung, improved the abundance of pulmonary microbiota, and enhanced immunity after exposure to low concentrations of ammonia.…”
Get full text

Condensed phase membrane introduction mass spectrometry: A new frontier for the real-time monitoring of hazardous chemical migration from food contact materials by Maurizio Piergiovanni, Veronica Termopoli, Cristian Maffezzoni, Nicolò Riboni, Viviana Consonni, Federica Bianchi, Monica Mattarozzi, Davide Ballabio, Maria Careri

“…A full factorial experimental design was applied to optimize five response variables by varying the acceptor phase flow-rate and composition, stirring, temperature, and membrane length. …”
Get full text

Advancing CH₄ and N₂O retrieval strategies for NDACC/IRWG FTIR observations with the support of airborne in situ measurements by I. Ortega, J. W. Hannigan, B. C. Baier, K. McKain, D. Smale

“…Ground-based high-resolution Fourier-transform infrared (FTIR) observations, employed by collaborative international initiatives like the Infrared Working Group (IRWG) within the Network for the Detection of Atmospheric Composition Change (NDACC), play a vital role in retrieving the atmospheric amounts of these gases. …”
Get full text

Preliminary Qualitative Analysis of Plant Samples by High-performance Thin-layer Chromatography for the Presence of Steroid Sapogenins of Some Representatives of the Dioscoreacae,... by A. E. Sukhanov, I. A. Krylov, V. V. Sepp, K. S. Bakulin

“…This work is exploratory in nature, assessing the presence of certain saponins by their sapogenins in selected extracts. We will optimize the HPLC separation procedure and choose other detection methods to unambiguously assess the co-presence of the studied sapogenins with approximately the same staining shades after derivatization and matching retention indices: there are pairs of steroidal sapogenins that have the same retention, we decided to group them in mixtures so that there would be separation within the group and subsequent comparison with the original extracts would make it possible to identify those or other sapogenins in such extracts.…”
Get full text

A novel motion key frame extraction and video stream classification based on reinforcement learning and feature fusion by Hongbo Cui, Tao Feng, Jinhui Zheng

“…Finally, the attention mechanism is used to calculate the importance of video frames, and reinforcement learning is used to extract and optimize key frames. The experimental results show that the new algorithm can solve the problem of error detection in the key frame extraction of motion video, and performs well in the detection of video frames containing key actions. …”
Get full text

A Spatial–Frequency Combined Transformer for Cloud Removal of Optical Remote Sensing Images by Fulian Zhao, Chenlong Ding, Xin Li, Runliang Xia, Caifeng Wu, Xin Lyu

“…A composite loss function, incorporating Charbonnier loss and Structural Similarity Index (SSIM) loss, is employed to optimize model training and balance pixel-level accuracy with structural fidelity. …”
Get full text

Greenness assessment profile of experimentally designed chromatographic method for the simultaneous analysis of ofloxacin and racecadotril in the presence of racecadotril impuritie... by Nada Nabil, Ghada A. Sedik, Hala E. Zaazaa, Sally S. El-Mosallamy

“…Experimental design using face-centred composite design was utilized during method optimization to obtain the highest level of resolution with the fewest number of experimental trials for the determination of ofloxacin (OFLU) and racecadotril (RAC) in the presence of RAC impurities. …”
Get full text

A Data Reconciliation-Based Method for Performance Estimation of Entrained-Flow Pulverized Coal Gasification by Yan Zhang, Kai Yue, Chang Yuan, Jiahao Xiang

“…Accurate performance estimation of the entrained-flow pulverized coal gasification unit is essential for production scheduling and process optimization, but these are often hindered by inaccurate or insufficient measurements in the industrial system. …”
Get full text

Multi-Template Molecularly Imprinted Polymers Coupled with a Solid-Phase Extraction System in the Selective Determination of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Envir... by David Aurelio-Soria, Giaan A. Alvarez-Romero, Maria E. Paez-Hernandez, I. Perez-Silva, Miriam Franco-Guzman, Gabriela Islas, Israel S. Ibarra

“…This methodology provides limits of detection from 3.00 to 12.00 µg L⁻¹ for the studied NSAIDs. …”
Get full text

Assessment of Microsatellite Genetic Diversity of Cultured Eriocheir sinensis Populations from Jiangsu and Anhui by Yuting HU, Jun LING, He JIANG, Huan WANG, Tingshuang PAN, Guoqing DUAN, Huaxing ZHOU, Min YANG, Tong LI

“…Furthermore, each population had a genetic composition similar to those of the three genetic lineage sources. …”
Get full text

Results of a Prospective Study Concerning the Clinical Efficiency of Recent Russian-Made Specialized Foods without Phenylalanine by T. V. Bushueva, T. E. Borovik, K. S. Ladodo, L. M. Kuzenkova

“…The chemical composition of the ration was controlled using dietology methods.Results: 57 children at the age of 14 days to 15 years with phenylketonuria (which was detected in neonatal screening) were picked for this study. …”
Get full text

Quantitative determination of the components of the mixture – ear drops “Melavet” by the method of high-performance liquid chromatography by V. V. Parchenko, D. Datsiuk, V. Hunchak, V. Martynyshyn

“…Therefore, the relevance of the research lies in finding the quantity of the composition of the created mixture – ear drops “Melavet” by the HPLC method with mass detection of the initial components. …”
Get full text

Multi-country and intersectoral assessment of cluster congruence between pipelines for genomics surveillance of foodborne pathogens by Verónica Mixão, Miguel Pinto, Holger Brendebach, Daniel Sobral, João Dourado Santos, Nicolas Radomski, Anne Sophie Majgaard Uldall, Arkadiusz Bomba, Michael Pietsch, Andrea Bucciacchio, Andrea de Ruvo, Pierluigi Castelli, Ewelina Iwan, Sandra Simon, Claudia E. Coipan, Jörg Linde, Liljana Petrovska, Rolf Sommer Kaas, Katrine Grimstrup Joensen, Sofie Holtsmark Nielsen, Kristoffer Kiil, Karin Lagesen, Adriano Di Pasquale, João Paulo Gomes, Carlus Deneke, Simon H. Tausch, Vítor Borges

“…Still, we identified non-negligible differences in outbreak detection and demonstrated how a threshold flexibilization favors the detection of similar outbreak signals by different laboratories. …”
Get full text

Relationship between Patterned Grounds Evolutions and Spatial and Temporal Changes in the Chemical Compounds of SAHL ABAD Playa Salt Flats (Case Study: Khorasan province) by Mahdi Saghafi

“…Therefore, in pattern grounds, with less changes or relatively stable of the playa surface, the chemical composition of gypsum is overcome, with the most significant patterns due to the presence of gypsum chemical composition. 4-Conclusion The evaluation of pattern grounds according to the changes made in chemical compounds to detect environmental changes or other changes in the playa surface is an important issue that can indicate the desertification status and the severity of the risks of the expansion of the playa. …”
Get full text

Enhancing wheat flour origin traceability by using laser-induced breakdown spectroscopy and Raman spectroscopy by Minghui Gu, Chao Liu, Hansong Huang, Xin Zhang, Jiguo Li, Qingbin Jiao, Liang Xu, Mingyu Yang, Xin Tan

“…With the increasing demand for the multi-dimensional composition detection and rapid traceability of wheat flour, this study presents the application of a combination of laser-induced breakdown spectroscopy (LIBS) and Raman Spectroscopy for traceability of wheat flour from five provinces of China, including Henan, Shanxi, Anhui, Shandong, and Hebei. …”
Get full text

Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota by Jana Mittelstrass, Renate Heinzelmann, René Eschen, Martin Hartmann, Quirin Kupper, Salome Schneider, Simone Prospero, Iva Franić

“…Conclusions Illumina and Oxford Nanopore Technologies metabarcoding captured similar host-dependent diversity patterns despite observed differences in numbers and composition of fungi. Short-read metabarcoding might be optimal for fungal biodiversity studies due to higher sequencing depths and resultant breadth of diversity. …”
Get full text

Energy-resolved neutron imaging and diffraction including grain orientation mapping using event camera technology by Tsviki Y. Hirsh, Andrew F. T. Leong, Adrian S. Losko, Alexander Wolfertz, Daniel J. Savage, Tim T. Jäger, John Rakovan, James J. Wall, Alexander M. Long, Sven C. Vogel

“…Energy-resolved imaging, on the other hand, offers both spatial and spectral information by detecting Bragg edges and neutron absorption resonances, which enables detailed mapping of microstructure and isotopic composition. …”
Get full text

Computational modeling of superparamagnetic nanoparticle-based (affinity) diagnostics by Loïc Van Dieren, Loïc Van Dieren, Loïc Van Dieren, Loïc Van Dieren, Loïc Van Dieren, Antoine A. Ruzette, Vlad Tereshenko, Haïzam Oubari, Haïzam Oubari, Haïzam Oubari, Yanis Berkane, Yanis Berkane, Jonathan Cornacchini, Jonathan Cornacchini, Filip Thiessen EF, Filip Thiessen EF, Filip Thiessen EF, Filip Thiessen EF, Curtis L. Cetrulo, Curtis L. Cetrulo, Curtis L. Cetrulo, Curtis L. Cetrulo, Korkut Uygun, Korkut Uygun, Alexandre G. Lellouch, Alexandre G. Lellouch, Alexandre G. Lellouch, Alexandre G. Lellouch, Alexandre G. Lellouch, Alexandre G. Lellouch

Get full text

Prospects of artificial intelligence for the sustainability of sugarcane production in the modern era of climate change: An overview of related global findings by Rajan Bhatt, Akbar Hossain, Debjyoti Majumder, Mandapelli Sharath Chandra, Rajiv Ghimire, Muhammad Faisal Shahzad, Krishan K. Verma, Amarinder Singh Riar, Vishnu D. Rajput, Mauro Wagner Oliveira, Adel Nisi, Riyadh S. Almalki, Viliam Bárek, Marian Brestic, Sagar Maitra

“…By analysing biochemical composition, assessing soil quality, projecting yields, predicting productivity, identifying illnesses, and predicting productivity, artificial intelligence (AI) has greatly improved sugarcane cultivation. …”
Get full text