FIRM image analysis: A machine learning workflow for quantifying extracellular matrix components from electron microscopy images. by Nicholas T Gigliotti, Justin Lee, Emily H Mang, Giancarlo R Zambrano, Mitra L Taheri

“…The extracellular matrix (ECM) is a complex network of biomolecules that plays an integral role in the structure, processes, and signaling mechanisms of cells and tissues. …”
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From isolation to detection, advancing insights into endothelial matrix-bound vesicles by Sahimy Ayus-Martinez, William Meza-Morales, Jesus Jimenez-Osorio, Maria Buendia-Otero, Luis López, Lisandro Cunci, Donald O. Freytes, Camilo Mora

“…Matrix-bound vesicles (MBVs), an integral part of the extracellular matrix (ECM), are emerging as pivotal factors in ECM-driven molecular signaling. …”
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Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique by Toru Hiratsuka, Emanuel Rognoni

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Entanglement microscopy and tomography in many-body systems by Ting-Tung Wang, Menghan Song, Liuke Lyu, William Witczak-Krempa, Zi Yang Meng

“…Here, we employ a protocol, dubbed entanglement microscopy, to reveal the multipartite entanglement encoded in the full reduced density matrix of the microscopic subregion in spin and fermionic many-body systems. …”
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Evaluation of lymphangiogenesis in acellular dermal matrix by Mario Cherubino, Igor Pellegatta, Federico Tamborini, Michele Cerati, Fausto Sessa, Luigi Valdatta

“…Results: We found remarkable wound contraction in defects that healed by secondary intention while minor wound contraction was observed in defects treated with ADM. At day 7, optical microscopy revealed a more plentiful cellularity in the granulation tissue compared with the dermal regeneration matrix. …”
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Label-free rat brain traumatic penumbra imaging based on multiphoton fluorescence microscopy by Shanghai Jiang, Pan Guo, Li Ai, Yuancan Li, Le Chen, Jie Zhong, Hua Yang, Hong Lu

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Open-top Bessel beam two-photon light sheet microscopy for three-dimensional pathology by Won Yeong Park, Jieun Yun, Jinho Shin, Byung Ho Oh, Gilsuk Yoon, Seung-Mo Hong, Ki Hean Kim

“…Herein, we developed open-top two-photon light sheet microscopy (OT-TP-LSM) for intraoperative 3D pathology. …”
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Leveraging automated time-lapse microscopy coupled with deep learning to automate colony forming assay by Anusha Klett, Anusha Klett, Dennis Raith, Dennis Raith, Paula Silvestrini, Paula Silvestrini, Paula Silvestrini, Matías Stingl, Jonas Bermeitinger, Avani Sapre, Avani Sapre, Avani Sapre, Martin Condor, Roman Melachrinos, Mira Kusterer, Alexandra Brand, Guido Pisani, Guido Pisani, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Marie Follo, Marie Follo, Jesús Duque-Afonso, Roland Mertelsmann, Roland Mertelsmann

“…To overcome these limitations, we developed an Artificial Intelligence (AI)-assisted automated CFA combining time-lapse microscopy for real-time tracking of colony formation.MethodsUsing B-acute lymphoblastic leukemia (B-ALL) cells from an E2A-PBX1 mouse model, we cultured them in a collagen-based 3D matrix with cytokines under static conditions in a low volume (60 µl) culture vessel and validated its comparability to methylcellulose-based media. …”
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Imaging pathology in archived cornea with Fuchs’ endothelial corneal dystrophy including tissue reprocessing for volume electron microscopy by Sayo Maeno, Philip N. Lewis, Robert D. Young, Yoshinori Oie, Kohji Nishida, Andrew J. Quantock

“…Here we report observations by light, transmission and volume scanning electron microscopy on changes in the endothelium and matrix associated with the characteristic deformations of Descemet’s membrane, termed guttae. …”
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The polymerization efficiency of a bulk-fill composite based on matrix-modification technology by Tarek M. Elshazly, Christoph Bourauel, Moustafa N. Aboushelib, Dalia I. Sherief, Dalia I. El-Korashy

“…ObjectivesTo evaluate the polymerization efficiency of a matrix-modified bulk-fill composite, and compare it to a conventional composite which has a similar filler system. …”
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Decellularised Sheep Tendon Derived Extracellular Matrix: An In-vitro Biochemical and Histological Analysis by Vazeeha Afrin Syed, S Balaji Ganesh, Gurumoorthy Kaarthikeyan, S Chitra

“…Introduction: Demineralised ovine cartilage is derived from sheep cartilage with its mineral content removed, preserving the collagen-rich organic matrix. It provides an osteoconductive scaffold that supports cell attachment, proliferation, and differentiation. …”
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Collectin-11 promotes fibroblast proliferation and modulates their activation status and extracellular matrix synthesis by Wan-Bing Chen, Wan-Bing Chen, Bo Cao, Gang Li, Gang Wang, Kun-Yi Wu, Ting Zhang, Ning Ma, Wuding Zhou, Ke Li, Ke Li

“…The immunofluorescence and molecular docking experiments were used to detect the binding of CL-11 to EGFR/TGFRII. ResultsIn this study, we demonstrate that CL-11 not only promotes fibroblast proliferation but also modulates their activation status and extracellular matrix (ECM) synthesis. …”
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Targeting the integrin beta 1‐focal adhesion kinase axis with artemisinin: Biophysical disruption of cell adhesion, migration, and invasion in tongue cancer by Laurensia Danis Anggradita, Joo Hyun Kim, Min‐Kyu Kim, Ji Won Son, Mohd Farhan, Jothilin Subitsha Alex Jeberson, Ali Taghizadeh, Hae‐Won Kim, Jae Hong Park, Jaemoon Yang, Hyung Kwon Byeon, Sung Sik Hur, Myung Jin Ban, Yongsung Hwang

“…Moreover, artemisinin treatment induces focal adhesion rearrangement and diminishes the cell's capacity to generate traction stress, consequently restraining cell migration on the matrix, as determined via traction force microscopy. …”
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STRUCTURAL MODIFICATION OF NEW FORMATIONS IN CEMENT MATRIX USING CARBON NANOTUBE DISPERSIONS AND NANOSILICA by B. M. Khroustalev, S. N. Leonovich, G. I. Yakovlev, I. S. Polianskich, O. Lahayne, J. Eberhardsteiner, G. Skripkiunas, I. A. Pudov, E. A. Karpova

“…Scanning electron microscopy and X-ray microanalysis with detection in IR spectra have revealed that adding of multi-walled carbon nanotubes dispersion together with nanodispersed silica provides structuring of rather dense shell of hydrated new formations along cement matrix on the surface of solid phase. …”
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Cryogenically structured gelatin-based hydrogel as a resorbable macroporous matrix for biomedical technologies by A. M. Grigoriev, Yu. B. Basok, A. D. Kirillova, V. A. Surguchenko, N. P. Shmerko, V. K. Kulakova, R. V. Ivanov, V. I. Lozinsky, A. M. Subbot, V. I. Sevastianov

“…Albumin secretion and ammonia metabolism when HepG2 cells were cultured on the gelatin sponge were detected. Conclusion. The use of a matrix made from macroporous cryogenically structured gelatin-based hydrogel for tissue engineering products is shown to be promising using a cell-engineered liver construct as a case.…”
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Assessment of the Presence of Microplastics in Stabilized Sewage Sludge: Analysis Methods and Environmental Impact by Jagoda Worek, Kamil Kawoń, Joanna Chwiej, Katarzyna Berent, Rosa Rego, Katarzyna Styszko

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Improving the mechanical properties of fiber-reinforced polymer composites through nanocellulose-modified epoxy matrix by Mustafa Kuyumcu, Cenk Kurtulus, Mustafa Ciftci, Mehmet Atilla Tasdelen

“…Additionally, the distribution of CNC in hybrid nanocomposites is also investigated by scanning and transmission electron microscopies. It is noted that the homogenous dispersion of CNCs in the epoxy matrix and their diameters varied from 10 to 100 nm are detected at higher magnification.…”
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KLF6-mediated glutamine metabolism governs odontogenic differentiation and matrix mineralization of dental pulp stem cells by Wenzhi Wu, Zekai Xu, Yulian Zhang, Xiatong Zhang, Xiaoyuan Huang, Zhijian Xie, Zhuo Chen

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The extracellular matrix protein type I collagen and fibronectin are regulated by β-arrestin-1/endothelin axis in human ovarian fibroblasts by Ilenia Masi, Flavia Ottavi, Valentina Caprara, Danila Del Rio, Martina Kunkl, Francesca Spadaro, Valerio Licursi, Loretta Tuosto, Anna Bagnato, Laura Rosano’

“…The expression of Col1 (COL1A1) and FN (FN1) were detected by western blotting (WB), quantitative real time-polymerase chain reaction (qRT-PCR), immunofluorescence (IF), and confocal laser scanning microscopy (CLSM) in cells and tumor tissue sections from mice xenografts, while the transcription of COL1A1 was detected by luciferase reporter gene assay. …”
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Microbial Biofilm Detection and Differentiation by Dual Staining Using Maneval’s Stain by B Nirmala, Balram Omar

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