The haplotype-resolved telomere-to-telomere genome and OMICS analyses reveal genetic responses to tapping in rubber tree by Chaochao Li, Yuan Yuan, Zhiyi Nie, Tingkai Wu, Zhiyuan Wang, Jiangxia Qiao, Zhi Deng, Xiaobo Wang, Dong Xu, Xu Wang, Shuo Cao, Bingqin Li, Zewei An, Wenguan Wu, Zhongxin Jin, Huasun Huang, Wei Hu, Yongfeng Zhou, Han Cheng

“…We present the haplotype-resolved, telomere-to-telomere, gap-free genome assembly of the cultivar CATAS 7-33–97, with both haplotypes containing complete telomeric and centromeric regions. Structural variations, including a 32.71 Mb inversion on chromosome 8, are identified. …”
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Unlocking winter maize potential: pioneering on-farm strategies for resilient yields in challenging climates by Raj Kumar Jat, Shubham Durgude, Vijay Singh Meena, Vijay Singh Meena, RK Sohane, RN Singh, Illathur R. Reddy, Suneel Kumar, S. Pazhanismy, Sunita Kumari Meena, Kumari Sharda, Susheel Singh, Rama Kant Singh, Rama Kant Singh, Seema Kumari, Seema Kumari, Pankaj Kumar, Govind Kumar, Amit Kumar Lenka, Asheesh Chaurasiya, Anup Das, Anil Kumar Jha, Dhananjay Pati Tripathi, Swati Sagar

“…The key factors evaluated for maize yields encompassed sowing windows, varietal performance, topography, seed treatment, earthing up, planting methods, spacing, tillage practices, irrigation, and nutrient management. Data was collected using a structured questionnaire that was validated by visiting on-farm experimentation at fields. …”
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Efficacy and safety of cannabidiol in a single-center pediatric drug-resistant epilepsy cohort: a retrospective study by Ambra Butera, Ambra Butera, Giulia Spoto, Graziana Ceraolo, Maria Grella, Ivana Giunta, Maria Ludovica Albertini, Carla Consoli, Caterina Sferro, Maria Spanò, Gabriella Di Rosa, Antonio Gennaro Nicotera

“…Clinical, demographic, and instrumental data were retrospectively extracted from the medical records and caregivers’ reports. …”
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Prognostic Role of Ultrasound Diagnostic Methods in Patients with Acute Decompensated Heart Failure by Zhanna D. Kobalava, Tolkacheva Veronika Vladimirovna, Sarlykov Bekbolot Kanatbekovich, Rena Sh Aslanova, Lapshin Artem Alekseevich, Nazarov Ivan Sergeevich, Smirnov Ilya Pavlovich, Maria V Vatsik-Gorodetskaya, Ghazaal Alavi Tabatabaei, Ibrahim Al-Zakwani, Mohammed Al Jarallah, Georgiana Luisa Baca, Peter A Brady, Rajesh Rajan, Bhavesh Talera

“…Clinical outcomes were assessed using a structured telephone survey method at 1, 3, 6, and 12 months after discharge. …”
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Conceptualizing the implementation of post-discharge malaria chemoprevention in Malawi using a co-design approach by Myness Kasanda Ndambo, Kristine Bærøe, Kamija Samuel Phiri, Isabel-Kazanga Chiumia, Inger Lise Teig, Bjarne Robberstad, Oddvar Martin Kaarbøe, Melf-Jakob Kühl, Jobiba Chinkhumba, Thandile Nkosi-Gondwe, Tinashe Alinafe Tizifa, Elisabeth Mamani-Mategula, Lumbani Munthali, Lucinda Manda-Taylor

“…Results The inductive analysis yielded four overarching themes from the data. These key themes are PDMC adaptability, trialability, implementability, and sustainability. …”
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Performance of GPT-4 for automated prostate biopsy decision-making based on mpMRI: a multi-center evidence study by Ming-Jun Shi, Zhi-Xiang Wang, Shuang-Kun Wang, Xuan-Hao Li, Yan-Lin Zhang, Ying Yan, Ran An, Li-Ning Dong, Lei Qiu, Tian Tian, Jia-Xin Liu, Hong-Chen Song, Ya-Fan Wang, Che Deng, Zi-Bing Cao, Hong-Yin Wang, Zheng Wang, Wei Wei, Jian Song, Jian Lu, Xuan Wei, Zhen-Chang Wang

“…GPT-4 generated structured reports with given prompts. The performance of GPT-4 was quantified using confusion matrices, and sensitivity, specificity, as well as area under the curve were calculated. …”
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A qualitative study on dietary behaviors and nutritional management needs of patients with active systemic lupus erythematosus (系统性红斑狼疮活动期患者饮食行为与营养管理需求的质性研究)... by SONG Weiting (宋炜婷), LI Yuwei (李雨蔚), QIAO Caicai (乔彩彩), WANG Sa (王飒), XUE Fei (薛菲), WANG Yuzhen (王玉珍), MIAO Huali (苗华丽)

“…Methods A descriptive study was conducted, involving semi-structured interviews with 10 patients with active SLE and 6 healthcare providers. …”
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Phenotypically distinguishable eosinophilic cells do not impact epithelial functions in a triple-culture in vitro intestinal model by Christoph Benkstein, Laurin Mosig, Daniel Vondran, Heidi Schlichting, Lea Kissing, Bente Wohlert, Ida Gensmer, Larissa Nogueira de Almeida, Peter König, Peter König, Kerstin Fibelkorn, Anna Kordowski, Stefanie Derer-Petersen, Christian Sina, Christian Sina, Yves Laumonnier, Yves Laumonnier

“…In-depth characterization by real-time PCR, flow cytometry, functional assay and electron microscopy showed that only butyrate and dibutyryl-cAMP generated phenotypically distinct eosinophilic cells with different activation statuses, marked by differential expression of surface markers CD11c and CD62L, increased expression of eosinophil specific genes, and development of eosinophilic structural features. Then, a triple-culture system encompassing the enterocytic cell line Caco-2 and the secretory cell line HT29-MTX complemented with eosinophilic differentiated cells was established. …”
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Feasibility study of single-image super-resolution scanning system based on deep learning for pathological diagnosis of oral epithelial dysplasia by Zhaochen Liu, Zhaochen Liu, Peiyan Wang, Peiyan Wang, Nian Deng, Hui Zhang, Hui Zhang, Fangjie Xin, Xiaofei Yu, Xiaofei Yu, Mujie Yuan, Mujie Yuan, Qiyue Yu, Yuhao Tang, Keke Dou, Jie Zhao, Bing He, Jing Deng, Jing Deng

“…Both the DS30R and Nikon scanners demonstrate high sensitivity and specificity for detecting structural features in OED pathological images; however, DS30R excels at identifying certain cellular features. …”
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Evidence based consensus statements for digital tools to address youth mental health literacy by Stephana Julia Moss, Sonia Siddiqui, Cristina Zuniga Chacon, Cynthia Sriskandarajah, Maia Stelfox, Ben Gaunce, Micaela Harley, Stacie Smith, Sofia B. Ahmed, Kathryn Birnie, Donna Halperin, Scott Halperin, Christine Hampson, Jia Hu, Laura Leppan, Angie Nickle, Kristine Russell, Andrea Soo, May Solis, Henry T. Stelfox, Sharon Straus, Perri R. Tutelman, Quincy Wiele, Kirsten M. Fiest, Nicole Racine, Jeanna Parsons Leigh

“…Phase II consisted of a modified Delphi consensus process to determine evidence-based consensus statements (N = 352 (13% youth) Round 1; N = 87 (33% youth) Round 2). Semi-structured focus groups in Phase III were conducted to refine the consensus statements (N = 16, 25% youth) and design the digital mental health literacy tool (N = 24, 33% youth). …”
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Quality of Surface and Groundwater in the Sierra de Amula Region, Jalisco, Mexico by Oscar Raúl Mancilla-Villa, Fabiola Villafaña-Castillo, Álvaro Can-Chulim, Rubén Darío Guevara-Gutiérrez, José Luis Olguín-López, Elia Cruz-Crespo, Jonas Alan Luna-Fletes, Juan Uriel Avelar-Roblero

“…Each sample was evaluated for its pH, electrical conductivity, and ionic concentration (Ca²⁺, Mg²⁺, Na⁺, K⁺, CO₃²⁻, HCO₃⁻, CI⁻, SO₄²⁻). For data analysis, we determined the ionic concentrations and the salinity and sodicity indexes, including the electrical conductivity, pH, sodium adsorption ratio (SAR), and cationic ratio of soil structural stability (CROSS). …”
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A qualitative analysis on the awareness of noise pollution and occupational protection among staff members of the Central Sterile Supply Department (消毒供应中心工作人员对噪声污染的认知及职业防护质性分析)... by HUANG Xuanxuan (黄玄玄), FU Li (付立), SUN Ziwen (孙子文), YU Jiang (于江), ZHANG Shenhong (张沈弘), ZHANG Tianqiang (张天强)

“…Methods Through face-to-face and semi-structured interviews, 10 staff members from the Central Sterile Supply Department were interviewed in depth. …”
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Risk factors for low knowledge and negative attitudes among caregivers of children with autism spectrum disorder in Iraq: a multi-centre cross-sectional study by Hiba Qahttan Khaleel Al-Juhaishi, Hiba Qahttan Khaleel Al-Juhaishi, Osamah Abbas Jaber, Osamah Abbas Jaber, Faris Lami, Faris Lami, Shatha Mohammed Jasim, Shatha Mohammed Jasim, Nahid Dehghan Nayeri, Nahid Dehghan Nayeri, Mahdi Shafiee Sabet, Mahdi Shafiee Sabet, Ghaith Al-Gburi, Ghaith Al-Gburi

“…Before educational interventions can address these issues, local knowledge and attitudes must be assessed, including the identification of high-risk groups that require prioritisation.MethodsBetween February 17 and September 10, 2024, Al-Subtain Academy for Autism and Baghdad’s National Centre for Autism and Child Psychiatry conducted a cross-sectional study. Data was collected using a structured questionnaire developed based on the Autism Stigma and Knowledge Questionnaire (ASK-Q) and a review of previous studies. …”
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Identifying the Components of Iranian Adolescent Compassionate Mind Training Program by Somayeh Mohebi, Iraj Shakerinia, Mahnaz Khosrojavid, Abbas Abolghasemi, Abbas Ali Hossein Khanzadeh:

“…Participants included university professors and school counsellors with extensive experience in relevant fields. Data was collected through semi-structured interviews, focusing on the essential components of compassion training for adolescents, distinguishing features of Iranian adolescents, and additional elements needed for cultural adaptation. …”
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Investigating the Mediating Role of Self-Efficacy in the Relationship between Job Demand and Role Ambiguity with Employee Well-Being by Mahnaz Rahimi, Mohammad Hassani, Hasen Ghalavand

“…</p> <p style="text-align: left;"><strong>3.Methodology</strong></p> <p style="text-align: left;">In this article, we intended to model the factors affecting employees' well-being with the mediation of self-efficacy using a quantitative approach and structural equation modeling. For this purpose, we first determined the independent, dependent and mediating variables based on the backgrounds. …”
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Impact of mealtime social experiences on student consumption of meals at school: a qualitative analysis of caregiver perspectives by Leah Elizabeth Chapman, Wendi Gosliner, Deborah A Olarte, Monica Daniela Zuercher, Lorrene D Ritchie, Dania Orta-Aleman, Marlene B Schwartz, Michele Polacsek, Christina E Hecht, Kenneth Hecht, Anisha I Patel, Punam Ohri-Vachaspati, Margaret Read, Juliana FW Cohen

“…Setting: Semi-structured interviews were conducted with United States (U.S.) caregivers over ZoomTM in English and Spanish during the 2021–2022 school year. …”
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Interpersonal and systemic factors in initiating, developing and maintaining collaborations between European neurosurgical departments and institutions in low-resources settings: A... by Nicolò Marchesini, Vicki M. Butenschoen, Andreas K. Demetriades, Said Idrissa Ahmada, Fazlul Hoque, Thomas Kapapa, Patrick D. Kamalo, Pablo González-López, Rupavathana Mahesperan, Ondra Petr, Wilco Peul, Nicephorus Boniface Rutabasibwa, Ellianne J. dos Santos Rubio, Abenezer Tirsit Aklilu, Jake Timothy, Enoch O. Uche, Magnus Tisell

“…Data were collected virtually between May and August 2024. …”
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Metabolic Acidosis Is Associated With an Accelerated Decline of Allograft Function in Pediatric Kidney Transplantation by Agnieszka Prytula, Rukshana Shroff, Ineke van Gremberghe, Kai Krupka, Justine Bacchetta, Elisa Benetti, Ryszard Grenda, Isabella Guzzo, Nele Kanzelmeyer, Bahar Büyükkaragöz, Birgitta Kranz, Hülya Nalçacıoğlu, Jun Oh, Lars Pape, Mohan Shenoy, Anne-Laure Sellier-Leclerc, Burkhard Tönshoff, Gema Ariceta, Atif Awan, Sevcan Bakkaloğlu, Marjolein Bonthuis, Charlotte Bootsma Robroeks, Antonia Bouts, Martin Christian, Marlies Cornelissen, Ali Duzova, Nasrin Esfandiar, Luciana Ghio, Ryszard Grenda, Isabella Guzzo, Maria Herrero Goni, Julien Hogan, Nattaphorn Hongsawong, Nele Kanzelmeyer, Aysun Karabay Bayazit, Gülşah Kaya Aksoy, Noel Knops, Linda Koster Kamphuis, Daniella Levy Erez, Victor Lopez-Baez, Alvaro Madrid, Stephen Marks, Anette Melk, Luisa Murer, Lars Pape, Licia Peruzzi, Edita Petrosyan, Evgenia Preka, Nikoleta Printza, Andreea Liana Rachisan, Ann Raes, Mohan Shenoy, Oguz Soylemezoglu, Luca Dello Strologo, Ana Teixeira, Rezan Topaloglu, Markus Weitz, Jakub Zieg, Galia Zlatanova, Christian Patry, Jerome Harambat, Ayşe Ağbaş, Varvara Askiti, Marina Avramescu, Justine Bacchetta, Sevcan Bakkaloglu, Marjolein Bontuis, Caroline Booth, Laurene Dehoux, Giacomo Dizazzo, Dorota Drozdz, Ismail Dursun, Michaela Gessner, Jaap Groothoff, Giuliana Guido, Isabella Guzzo, Aysun Karabay Bayazit, Guenter Klaus, Linda Koster-Kamphuis, Alexander Lalayiannis, Maren Leifheit-Nestler, Sinha Manish, Chiara Matteucci, Jun Oh, Ozan Ozkaya, Edita Petrosyan, Christine Pietrement, Agnieszka Prytula, George Reusz, Franz Schaefer, Claus Peter Schmitt, Anne Schön, Fatma Lale Sever, Stella Stabouli, Serra Sürmeli Döven, Camilla Tondel, Enrico Verrina, Enrico Vidal, Dean Wallace, Zainab Arslan, M. Bald, H. Fehrenbach, D. Haffner, M. Hansen, C. Hempel, U. John, G. Klaus, J. König, B. Lange-Sperandio, D. Müller, J. Oh, L. Pape, M. Pohl, K. Sauerstein, G. Schalk, H. Staude, P. Strotmann, L.T. Weber, M. Weitz, L. Berta, K. Heindl-Rusai

“…Methods: This registry study collected data up to 10 years posttransplant. Survival analysis for a composite end point of graft loss or estimated glomerular filtration rate (eGFR) ≤ 30 ml/min per 1.73 m2 or ≥50% decline from eGFR at month 3 posttransplant was performed. …”
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Epithelial-to-mesenchymal transition is an active process in the large airways of patients with asthma-COPD overlap and partially abrogated by inhaled corticosteroid treatment: a b... by Surajit Dey, Wenying Lu, Prabuddha S. Pathinayake, Maddison Waters, Greg Haug, Josie Larby, Heinrich C. Weber, Peter A. B. Wark, Peter A. B. Wark, Mathew Suji Eapen, Sukhwinder Singh Sohal

“…Additionally, the degree of RBM fragmentation was evaluated, a key tissue structural marker of EMT.ResultsCompared to healthy controls and asthmatics, ACO had the greatest fragmentation of RBM (P &lt; 0.01). …”
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Factors associated with knowledge, attitudes, and practices of mixed crop-livestock farmers on Crimean-Congo hemorrhagic fever (CCHF) and other zoonoses in Burkina Faso by Abdoul Kader Ilboudo, Michel Dione, Ard M. Nijhof, Martin H. Groschup, Ousmane Traoré, Guy S. Ilboudo, Zekiba Tarnagda, Madi Savadogo, Bernard Bett

“…Consenting participants aged six and above were randomly included, and a structured questionnaire that collected socio-economic data, knowledge, attitudes, and practices concerning CCHF and other zoonoses was administered. …”
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