Technical note: Flow cytometry assays for the detection, counting and cell sorting of polyphosphate-accumulating bacteria by C. Bouquet, H. Billard, H. Billard, C. C. Bidaud, J. Colombet, J. Colombet, Y.-T. Chang, J. Artigas, I. Batisson, K. Benzerara, F. Skouri-Panet, E. Duprat, A.-C. Lehours

“…This requires high-throughput methods to characterize their structure and dynamics in ecosystems. A promising strategy is to combine the staining of intracellular polyphosphate granules and their subsequent detection by flow cytometry, enabling rapid data acquisition. …”
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Contextual factors in value-based decision support to enhance health technologies adoption: the case of biosimilars by Maximilian Otte, Maximilian Otte, Maximilian Otte, Zoltán Kaló, Zoltán Kaló, Zoltán Kaló, Hussain Abdulrahman Al-Omar, Meindert Boysen, Yingyao Chen, Ana Paula Beck Da Silva Etges, Ana Paula Beck Da Silva Etges, Guvenc Kockaya, Iñaki Gutierrez-Ibarluzea, Ahmed Mohammed Seyam, Deirdre Mullen, Deirdre Mullen, Hans-Peter Dauben, Hans-Peter Dauben, Hans-Peter Dauben

“…The MCDA framework offers a structured approach to operationalize these principles. …”
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Erythritol as a Next-Generation Sweetener: properties, metabolism, cardiovascular risk and gut health implications – A literature review by Julia Skowrońska-Borsuk, Martyna Narożniak, Adrianna Ewa Pękacka, Bartłomiej Czerwiec, Adam Borsuk, Joanna Katarzyna Pergoł, Malwina Wojtas, Julia Borkowska, Zuzanna Krupa, Julia Sposób

“…However, these findings are inconclusive and require further investigation. Emerging animal data indicate erythritol may modulate gut epithelial structure via microbiota-derived metabolites. …”
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Anti-Migratory Activity of Brazilin Chemodiversification on Breast Cancer Cells by Alberto Hernández-Moreno, Dania A. Nava-Tapia, Miriam D. Zuñiga-Eulogio, Jorge Bello-Martínez, Monserrat Olea-Flores, Tadeo Hernández-Moreno, Mario Ordoñez, Ana E. Zacapala-Gómez, Miguel A. Mendoza-Catalán, Napoleón Navarro-Tito

“…In this study, we isolated brazilin from the heartwood of <i>Haematoxylum brasiletto</i>; then, we performed a semi-synthesis by adding three methyl or acetyl groups to the core structure of brazilin. We confirmed the identity of brazilin and its derivatives by spectroscopic data (¹H NMR and ¹³C NMR) and measured their purity by optical rotation. …”
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An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies by Chenfang Miao, Menghan Zhang, Ying Zheng, Xiaoyan Lin, Shuangying Yang, Linlin Xu, Sitong Lu, Zhengjun Huang, Jianyong Huang, Yanjie Zheng, Shaohuang Weng

“…In this study, high quantum yield carbon dots (CA/EDA-CDs) were synthesized via a hydrothermal method using riboflavin as the carbon source, citric acid and ethylenediamine as functionalizing agents and dopants. The luminescent structure of CA/EDA-CDs, featuring a π-conjugated system (aromatic C=C and graphite N) and surface chromophores (C=N/C=O) connected via C-N, C-O, and hydrogen bonds, was controllably modulated by hydroxyl radical (·OH). …”
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Orthopaedic Consultation is Associated With Fewer Patient-Perceived Barriers to Total Joint Arthroplasty by Insa Mannstadt, BS, BA, J. Alex B. Gibbons, BA, Troy B. Amen, MD, MBA, Mangala Rajan, PhD, Sarah R. Young, PhD, Henry Tischler, MD, Michael L. Parks, MD, Mark Figgie, MD, Anne Bass, MD, Linda Russell, MD, Bella Mehta, MD, MS, Iris Navarro-Millán, MD, Susan M. Goodman, MD

“…Methods: We performed a post-hoc analysis of questionnaire responses based on data from a previous study that used semi-structured interviews with patients with advanced osteoarthritis. …”
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ASSESSMENT OF THE PSYCHOLOGICAL STATUS OF PATIENTS WITH GYNECOLOGICAL CANCER IN THE PROCESS OF PSYCHOLOGICAL REHABILITATION by E. V. Pestereva, V. A. Chulkova, T. Y. Semiglazova, E. G. Ulrich, O. A. Pestereva

“…Disturbing component in patients at the stage of «before operation» and dysphoric component in patients at the stage of «after surgery» were dominated on the background of ergopathic reactions to the disease in the structure of attitude to the disease. The high intensity of complaints of female patients about their health, including non-specific, indicating the psychosomatic component of the patient’s suffering was revealed. …”
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Inaccuracies of venous thromboembolism risk assessment and prevention practices among medically ill patients by Brandyn D. Lau, Aditya Bhave, Jennifer C. Yui, Rakhi Naik, Kathryn E. Dane, John Lindsley, Dauryne L. Shaffer, Peggy S. Kraus, Erik H. Hoyer, Elliott R. Haut, Michael B. Streiff

“…Leveraging existing structured data to autopopulate VTE risk assessments can assist providers in improving accuracy. …”
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Fundamental Parameters for Totally Eclipsing Contact Binaries Observed by TESS by Xu Ding, KaiFan Ji, ZhiMing Song, XueFen Tian, JinLiang Wang, ChuanJun Wang, QiYuan Cheng, JianPing Xiong

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Investigating an Erupting Metric-decimetric Radio Depression and Its Physical Origin by B. T. Wang, X. Cheng, J. Y. Yan, C. Xing, W. T. Fu, L. Wu, L. Deng, A. L. Lan, Y. Chen, C. Wang, M. D. Ding

“…At the radio band, even though the filament is difficult to identify in its early phase, it rapidly became distinct and formed a continuous loop-like dark structure during the eruption, i.e., so-called radio depression. …”
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Occupational health and safety regulations in China: development process, enforcement challenges, and solutions by Xinyu Xu, Xinyu Xu, Xinyu Xu, Peihua Ren, Peihua Ren, Wenyan Shi, Wenyan Shi, Feidan Deng, Feidan Deng, Qingfeng Wang, Qingfeng Wang, Shaoteng Shi, Shaoteng Shi, Jingchun Shen, Jingchun Shen, Guoqiang Dong, Jing Han, Jing Han, Jing Han, Jing Han

“…The purpose of this paper is to summarize the current health status of China’s occupational population and the dilemmas faced by prevention and treatment work.MethodsThe following databases were utilized: China National Knowledge Infrastructure (CNKI), Wanfang Data, PubMed, Web of Science, Scopus, and Google Scholar. …”
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Unveiling the charge density wave mechanism in vanadium-based Bi-layered kagome metals by Yi-Chen Yang, Soohyun Cho, Tong-Rui Li, Xiang-Qi Liu, Zheng-Tai Liu, Zhi-Cheng Jiang, Jian-Yang Ding, Wei Xia, Zi-Cheng Tao, Jia-Yu Liu, Wen-Chuan Jing, Yu Huang, Yu-Ming Shi, Soonsang Huh, Takeshi Kondo, Zhe Sun, Ji-Shan Liu, Mao Ye, Yi-Lin Wang, Yan-Feng Guo, Da-Wei Shen

“…Our finding not only comprehensively maps the electronic structure of V-based bi-layer kagome metals but also provides constructive experimental evidence for the unique origin of CDW in this system.…”
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Physical activity, screen time, and sleep quality of students during remote learning by Elias dos Santos Batista, Helder Xavier Bezerra, Gabriel Pereira Maciel, Alesandra Araújo de Souza, Marcela Rodrigues de Castro, Valter Cordeiro Barbosa Filho, Rodrigo Ramalho Aniceto

“…An online questionnaire was structured, including recall information about behaviors before and during remote learning. …”
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Optimal suturing techniques in patch-bridging reconstruction for massive rotator cuff tears: A finite element analysis by Yuting Zhong, Chengxuan Yu, Sijia Feng, Han Gao, Luyi Sun, Yunxia Li, Shiyi Chen, Jun Chen

“…The ultimate failure load, failure mode, stress distribution of each structure, and other biomechanical results of the different models were calculated and compared. …”
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Statistical modeling of the equine third metacarpal bone incorporating morphology and bone mineral density. by Helen Liley, Ju Zhang, Elwyn C Firth, Justin W Fernandez, Thor F Besier

“…The association between form and function builds upon previous two-dimensional observations of MC3 epiphyseal structure. It was expected that the main source of variation would be an increase in overall MC3 bone size, correlated to an increase in subchondral BMD. …”
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The role of offensive and creative priming videos in enhancing youth football players’ performance during small-sided games by Diogo Coutinho, Diogo Coutinho, Bruno Gonçalves, Bruno Gonçalves, Sara Santos, Sara Santos, Sigrid Olthof, Rafael Ballester Lengua, André Roca, Bruno Travassos, Bruno Travassos, Bruno Travassos

“…Coaches can leverage offensive priming to improve structured passing and positioning, while creative priming may inspire exploratory movements and innovative shooting attempts.…”
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Remote-sensing-based forest canopy height mapping: some models are useful, but might they provide us with even more insights when combined? by N. Besic, N. Picard, C. Vega, J.-D. Bontemps, L. Hertzog, J.-P. Renaud, J.-P. Renaud, F. Fogel, M. Schwartz, A. Pellissier-Tanon, G. Destouet, F. Mortier, F. Mortier, M. Planells-Rodriguez, P. Ciais

“…<p>The development of high-resolution mapping models for forest attributes based on remote sensing data combined with machine or deep learning techniques has become a prominent topic in the field of forest observation and monitoring. …”
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Transition from child to adult health services for young people with cerebral palsy in Ireland; implications from a mixed-methods study [version 2; peer review: 2 approved] by Owen Hensey, Amanda Breen, Aisling Walsh, Mary Owens, Claire Kerr, Sebastian Koppe, Grace Lavelle, Meriel Norris, Michael Walsh, Jennifer M. Ryan, Thilo Kroll, Jennifer Fortune

“…Methods A convergent parallel mixed-methods study was conducted. Data were collected from people with cerebral palsy aged 16–22 years, parents, and health professionals using surveys and semi-structured interviews, which were both informed by a framework of nine key transition practices. …”
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Investigation of an extensive outbreak of HIV infection among children in Sindh, Pakistan: protocol for a matched case–control study by Victoria Simms, Rashida Ferrand, Baseer Achakzai, Iqbal Azam, Amna R Siddiqui, Apsara Ali Nathwani, Syed H Abidi, Syed Faisal Mahmood, Sobiya Sawani, Abdul M Kazi, Aneeta Hotwani, Sikander Ali Memon, Jamila Soomro, Saqib Ali Shaikh, Quaid Saeed, Palwasha Khan, Fatima Mir

“…Following written informed consent from the guardian, a structured questionnaire will be administered to collect data on sociodemographic indices and exposure to risk factors for parenteral, vertical and sexual (only among those aged above 10 years) HIV transmission. …”
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Optimising Paediatric Transition to Intensive Care for Adults (OPTICAL): study protocol for a mixed method study by Padmanabhan Ramnarayan, Louise Rose, Julie Taylor, Christina Pagel, Jo Wray, Sarah E Seaton, Kate Oulton, Sonya Crowe, Rum Thomas, Qi Huang, Paul R Mouncey, Katherine Malbon, Anjalika Mallick, Charmaine Kohn, Sue Ben Abraham, Clare Windsor

“…To do this, we will: (1) link and analyse UK national data (years 2017–2024) on paediatric and adult ICU admissions, hospital inpatient, outpatient and emergency care visits and survival status, to determine the clinical characteristics and healthcare resource utilisation from teenage years to early adulthood of people admitted to an ICU as a young person (admission aged 14 and 15), and how these relate to ICU admissions after age 16; (2) conduct semistructured interviews, online forums and surveys with TYA patients, carers and health professionals to understand their experience of transition in ICU services; and (3) synthesise these strands of evidence and use a structured process of stakeholder engagement to propose potential targeted improvements as appropriate.Ethics and dissemination This study was approved by the East of England - Cambridge South Research Ethics Committee on 1 August 2024 (research ethics committee number 24/EE/0108), and the Health Research Authority Confidentiality Advisory Group (CAG) on 7 October 2024 (CAG number 24/CAG/0068). …”
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