Selection and validation of stable reference genes in guava (Psidium guajava L.) for reliable and consistent gene expression analysis by Sandeep Kumar, Manoharan Muthukumar, Anju Bajpai, Amar Kant Kushwaha, Israr Ahmad, Yashi Bajpai, Anshuman Singh, Thukkaram Damodaran, Mala Trivedi

“…Background: Ideal stably expressing housekeeping gene to be used as internal control needs to be optimized for efficient gene expression analysis for accurate mRNA quantitation. …”
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Clinical potential and experimental validation of prognostic genes in hepatocellular carcinoma revealed by risk modeling utilizing single cell and transcriptome constructs by Hang Deng, Xu Wang, Zi-Ang Jiang, Jian Xu, Yu Zhang, Yao Zhou, Jun Gong, Xiang-Yu Lu, Yi-Fu Hou, Hao Zhang

“…B cells were identified as key cellular components with the highest interaction numbers and strengths with macrophages in both HCC and control groups. Prognostic genes were more highly expressed in the initial state of B cell differentiation. …”
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Development of a Real-time Near-surface Air Temperature, Humidity, and Wind Product with 10-min Resolution in China by Liao Zhihong, Han Shuai, Gu Junxia, Shi Chunxiang, Zhang Zhiqiang, Ruan Xin, Zhao Qi, Zhang Tao, Zhang Zhisen, Du Aodong, Xu Bin, Sun Shuai, Pang Zihao, Zhang Yu, Xu Yanqin, Yang Lei

“…Results indicate that these near-surface air temperature, relative humidity and wind in 10-min product can more finely characterize the process of these extreme weather events when compared with 1-h products, which improve the capability of meteorological operations from hourly to minutely level. …”
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Effect of Spirulina on the Rumen Microbiota and Serum Biochemical Parameters of Lambs by Zhijun Wang, Xiangdong Liu, Muqier Zhao, Weiqin Ma, Yuxuan Wang, Yushan Jia, Gentu Ge

“…These results suggest that the addition of Spirulina is more beneficial for serum biochemical parameters and rumen microbiota of lambs. …”
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Self-monitoring blood pressure in pregnancy: evaluation of women’s experiences of the BUMP trials by Alison Chisholm, Katherine L Tucker, Carole Crawford, Marcus Green, Sheila Greenfield, James Hodgkinson, Layla Lavallee, Paul Leeson, Lucy Mackillop, Christine McCourt, Jane Sandall, Hannah Wilson, Lucy C Chappell, Richard J McManus, Lisa Hinton

“…In the usual care arm, more women were already self-monitoring their blood pressure than expected. …”
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Duration of dam contact had a long effect on calf rumen microbiota without affecting growth by Laurianne Voland, Abimael Ortiz-Chura, Jeremy Tournayre, Bruno Martin, Matthieu Bouchon, Alessandra Nicolao, Alessandra Nicolao, Dominique Pomiès, Diego P. Morgavi, Milka Popova

“…We postulated that 4 weeks of cow-calf contact, a potentially more socially acceptable, and economically pragmatic, management practice, would induce a similar development of ruminal microbiota to that observed with separation at weaning, thereby conferring benefits on calf health and growth.MethodsWe studied three groups of 14 cow-calf pairs (Holstein and Montbéliarde breeds) with different cow-calf separation times: 4 weeks of contact with the mother (Mixed group), immediate separation (at birth, Control group) and delayed separation at weaning (11 weeks, Dam group). …”
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Dynamic selectout and voting-based federated learning for enhanced medical image analysis by Saeed Iqbal, Adnan N Qureshi, Musaed Alhussein, Khursheed Aurangzeb, Atif Mahmood, Saaidal Razalli Bin Azzuhri

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Improvement of accuracy and diagnostic significance of breast tumor fine-needle aspiration biopsy by miRNA analysis of material isolated from cytological smears by N. N. Kolesnikov, S. E. Titov, Yu. A. Veryaskina, A. V. Vladimirova, R. B. Samsonov, A. S. Artem’eva, V. I. Novik, L. M. Bershteyn, I. F. Zhimulev, A. V. Malek

“…MicroRNAs (miRNAs), regulatory molecules involved in control of virtually all physiologic and pathologic process, emerged as promising tumor markers. …”
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Association of dietary cholesterol intake with the risk of fatal cardiovascular events in individuals with type 2 diabetes by A. K. Kuntsevich, O. D. Rymar, S. V. Mustafina, G. I. Simonova, L. V. Shcherbakova, E. G. Verevkin, A. O. Shchetinina, S. K. Malyutina

“…The results of this study may suggest a possible association of high-cholesterol foods with an increased risk of death in people with type 2 diabetes, pointing to the need for people with diabetes to control their dietary cholesterol levels and more optimal type of diet for this population.…”
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Biochar Co-Applied with Lime Enhances Soil Phosphorus Availability via Microbial and Enzymatic Modulation of Paddy Soil by Yang Zhang, Caidi Yang, Jun Wang, Shenggao Lu

“…Biochar co-applied with lime increased the available P (Olsen-P by 169–209%) and inorganic P (Al-P by 53.4–161%, Fe-P by 96.3–198%, and Ca-P by 59.0–154%) more than biochar alone, compared to the control (CK). …”
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On the advisability of long-term use of low-dose glucocorticoids in elderly patients with rheumatoid arthritis by A. V. Gordeev, E. V. Matyanova, E. G. Zotkin, A. M. Lila

“…Long-term use of low-dose GCs in elderly RA patients was not associated with improved disease control in terms of disease activity or radiographic progression, nor with rational use of csDMARDs. …”
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Which novel teaching strategy is most recommended in medical education? A systematic review and network meta-analysis by Shuai-Long Zhang, Si-Jing Ren, Dong-Mei Zhu, Tian-Yao Liu, Lian Wang, Jing-Hui Zhao, Xiao-Tang Fan, Hong Gong

“…Abstract Aim There is no conclusive evidence which one is the optimal methodology for enhancing the quality and efficacy of learning for medical students. …”
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Impact Mechanisms of Different Ecological Forest Restoration Modes on Soil Microbial Diversity and Community Structure in Loess Hilly Areas by Gang Chen, Jinjun Cai, Weiqian Li, Yitong Liu, Yan Wu, Tongtong Wang

“…This work is mainly aimed at exploring a more reasonable and efficient ecological forest restoration mode and revealing synergistic restoration mechanisms. …”
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Effect of microbial fertilizers on soil microbial community structure in rotating and continuous cropping Glycyrrhiza uralensis by Daiyu Qiu, Daiyu Qiu, Xue Wang, Xue Wang, Kan Jiang, Kan Jiang, Gaoxia Gong, Gaoxia Gong, Fang Bao

“…The complex microbial agent significantly increased the relative abundance of soil dominant bacteria communities of continuous cropping and rotating G. uralensis by 3.11~11.54 percentage points, and significantly reduced the relative abundance of soil dominant fungal communities of continuous cropping G. uralensis by 1.57~8.93 percentage points, compared with the control. Of the three microbial fertilizers, the complex microbial agent had the most significant effects on optimizing the soil microbial community structure of continuous cropping and rotating G. uralensis. …”
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Correcting Tibiotalar Alignment Correlates with FAO Changes after Total Ankle Replacement by Ricardo Villar MD, Emily Teehan MS, Kira Lu, Ranqing Lan MS, Patricia Friedmann MS, Jensen Henry MD, Constantine Demetracopoulos MD

“…Submission Type: Ankle Arthritis Research Type: Level 3 - Retrospective cohort study, Case-control study, Meta-analysis of Level 3 studies Introduction/Purpose: Re-establishing anatomic ankle and foot alignment is critical to optimize clinical outcomes and longevity of total ankle replacement (TAR). …”
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Combined CT-based extracellular volume with pathologic indicators predicts early postoperative recurrence of gastric mesenchymal tumors by LU Hong, LU Hong, LI Haitao, CAI Ping

“…Methods A retrospective case-control trial was conducted on the imaging, clinical and pathological data of 110 patients with gastric mesenchymal tumors who were surgically resected at the First Affiliated Hospital of Army Medical University from January 2011 to August 2022. …”
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Utility of extended HPV genotyping for the triage of self-sampled HPV-positive women in a screen-and-treat strategy for cervical cancer prevention in Cameroon: a prospective study... by Pierre Vassilakos, Evelyn Tincho, Bruno Kenfack, Patrick Petignat, Ania Wisniak, Celine Broquet, François Marcel Ndam Nsangou, Michel Noubom, Emilien Jeannot

“…Objective To explore the utility of extended Human Papillomavirus (HPV) genotyping to detect cervical intraepithelial neoplasia grade 2 or more (CIN2+) in a ‘screen-and-treat’ strategy for HPV-positive women in low-resource settings.Design Prospective study of diagnostic accuracy.Setting The study took place in West Cameroon between September 2018 and March 2020.Participants 2014 women were recruited. …”
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Improving AI-Based Clinical Decision Support Systems and Their Integration Into Care From the Perspective of Experts: Interview Study Among Different Stakeholders by Godwin Denk Giebel, Pascal Raszke, Hartmuth Nowak, Lars Palmowski, Michael Adamzik, Philipp Heinz, Marianne Tokic, Nina Timmesfeld, Frank Martin Brunkhorst, Jürgen Wasem, Nikola Blase

“…Suggestions were heterogeneous and concerned improvements: (1) in the systems themselves (eg, implementing comprehensive system training involving [future] users; using a comprehensive and high-quality database; considering usability, transparency, and customizability; preventing automation bias through control mechanisms or intelligent design; conducting studies to demonstrate the benefit of the system), (2) on the user side (eg, training [future] physicians could contribute to a more positive attitude and to greater awareness and questioning decision supports suggested by the system and ensuring that the use of the system does not lead to additional work), and (3) in the environment in which the systems are used (eg, increasing the digitalization of the health care system, especially in hospitals; providing transparent public communication about the benefits and risks of AI; providing research funding; clarifying open legal issues, eg, those related to liability; and standardizing and consolidating various approval processes). …”
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Adjusting Aspergillus niger pellet diameter, population heterogeneity, and core architecture during shake flask cultivation by K. Engelbert, C. Deffur, T. C. Cairns, F. Zhang, T. Kheirkhah, H. Winter, S. Junne, P. Neubauer, H. Briesen, V. Meyer

“…Results In this study, we have developed shake flask cultivation conditions which enable reproducible macromorphological control of the multipurpose cell factory Aspergillus niger. …”
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Enhancing Soil Phosphorus Availability in Intercropping Systems: Roles of Plant Growth Regulators by Chunhua Gao, Weilin Kong, Fengtao Zhao, Feiyan Ju, Ping Liu, Zongxin Li, Kaichang Liu, Haijun Zhao

“…Under a 4-row corn and 6-row soybean strip intercropping system, three treatments were applied: a water control (CK), and two plant growth regulators—T1 (EC: ethephon [300 mg/L] + cycocel [2 g/L]) and T2 (ED: ethephon [300 mg/L] + 2-Diethyl aminoethyl hexanoate [10 mg/L]). …”
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