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    Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production by Yufei Dong, Zhen Chen

    Published 2025-06-01
    “…This study presents a comprehensive metabolic engineering approach to establish robust C. glutamicum strains for l-tryptophan biosynthesis, including: (1) identification of potential targets by enzyme-constrained genome-scale modeling; (2) enhancement of the l-tryptophan biosynthetic pathway; (3) reconfiguration of central metabolic pathways; (4) identification of metabolic bottlenecks through comparative metabolome analysis; (5) engineering of the transport system, shikimate pathway, and precursor supply; and (6) repression of competing pathways and iterative optimization of key targets. …”
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    Treatment of inoperable pancreatic adenocarcinoma with focused ultrasound and microbubbles in patients receiving chemotherapy by Margrete Haram, Rune Hansen, Ola Finneng Myhre, Stian Solberg, Naseh Amini, Bjørn Atle Angelsen, Catharina de Lange Davies, Eva Hofsli

    Published 2025-06-01
    “…Nine boluses of MBs were injected intravenously (i.v) with a 3.5 min interval. Patients were scheduled for two months of treatment. …”
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    Rational Design for Improving the Thermostability of Saccharomyces cerevisiae Nicotinamide Riboside Kinase 1 by WANG Yao, SHEN Taisong, LI Sichen, SHI Hongling, YAO Lunguang, TANG Cunduo

    Published 2024-12-01
    “…The results showed that after two rounds of mutagenesis, a mutant named T136P/S209A with significantly improved thermal stability and catalytic activity was obtained. Its optimal reaction temperature was increased to 45 ℃, and its half-life at 45 ℃ was 48.98 min, which was 4.2 times as high as that of the wild-type ScNRK1. …”
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