Cross-lineage 5-methylcytosine methylome profiling reveals methylated divergence among Toxoplasma gondii tachyzoites of the three major clonal lineages by Xiao-Nan Zheng, Hong-Yu Song, Hany M. Elsheikha, Chen-Ran Tian, Xing Tian, Qing Liu, Wen-Bin Zheng, Xing-Quan Zhu

“…Among the emerging layers of gene regulation, 5-methylcytosine (m5C) has emerged as a pivotal post-transcriptional modification in eukaryotes. …”
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Single-cell transcriptomics reveals cellular dynamics and chemokine CXCL2-mediated smooth muscle cell proliferation in arterial repair by Xingxiao Huang, Xingxiao Huang, Yan Ping, Yan Ping, Qiuli Sun, Qiuli Sun, Mingjun Yu, Mingjun Yu, Chao Yang, Xiao Liu, Long Wang, Jinyu Huang, Jinyu Huang

“…And distinct subpopulations within these cell types were identified, each exhibiting unique functional characteristics and temporal dynamics during repair. (2) Notably, we identified an inflammatory SMC subpopulation (SMC2) that actively secretes chemokine CXCL2, which promotes SMC proliferation and mediates arterial remodeling after injury, suggesting its potential as a therapeutic target.ConclusionsThis study provides a comprehensive cell atlas of the injured artery, offering valuable insights into the complex cellular interactions, signaling pathways and immune responses involved in orchestrating vascular repair. …”
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Knockout of thyroid hormone receptor alpha a (thraa) enhances cardiac regeneration in zebrafish through metabolic and hypoxic regulation by Man Yee Cheung, Chunmei Jiang, Imtiaz Ul Hassan, Hui Wang, Donghao Guo, Daniel Wuyang Dio, Huan Yan, Jianmin Sun, Xufeng Qi, Dongqing Cai, Wei Ge, Sheue-yann Cheng, Wai-Yee Chan, Hui Zhao

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Identification and Mechanistic Studies of Key Genes in Thalamic Hemorrhage Pain by Multi-omics by Chen Yang, Ju Gao, Yaqun Li, Yinggang Xiao, Tianfeng Huang

“…Background: Thalamic hemorrhage pain (THP), a subtype of central post-stroke pain (CPSP), commonly develops following ischemic or hemorrhagic injury to the thalamus. …”
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The influence of evidence-based nutritional support plans on the nutritional status and adverse effects of radiotherapy in individuals with nasopharyngeal carcinoma by Xiaomei Fan, Xiaomei Fan, Huixia Cui, Haibo Peng, Haibo Peng, Shasha Liu, Li Jiang

“…All patients completed radiotherapy, and no significant difference was observed in radiotherapy interruption rates between groups (control group: 6 interruptions; intervention group: 1 interruption; p > 0.05). Post-radiotherapy QoL scores demonstrated that the intervention group achieved superior outcomes in physical, role, emotional, cognitive, and social functioning (p < 0.05).ConclusionImplementing evidence-based nutritional support programs has the potential to prevent the decline in nutritional status among NPC patients receiving radiotherapy, reduce the occurrence of treatment-related side effects, and enhance overall quality of life.…”
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The features of the human mesenteric lymph node histogenesis in the first half of the prenatal period of ontogenesis by M. I. Dub, , E. G. Alieva

“…Features of the mesenteric lymph nodes morphogenesis are not well understood due to the different functions of these organs [1, 2]. There is still no consensus on the initial stages of the mesenteric lymph nodes. …”
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Improving reading competence in aphasia with combined aerobic exercise and phono-motor treatment: Protocol for a randomized controlled trial. by Olga Boukrina, Elizabeth B Madden, Brian M Sandroff, Xiangqin Cui, Abubakar Yamin, Yekyung Kong, William W Graves

“…The study is structured as a Phase I/II clinical trial and compares the effects of AET plus PMT to a control condition of stretching plus PMT on reading and other language outcomes including naming, auditory comprehension, and spontaneous speech. Additionally, it investigates the immediate and sustained impacts of the intervention on CBF, functional connectivity, and task-evoked brain activity. …”
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Understanding the effectiveness and underlying mechanisms of lifestyle modification interventions in adults with learning disabilities: a mixed-methods systematic review by Dikshyanta Rana, Sophie Westrop, Nishant Jaiswal, Evi Germeni, Arlene McGarty, Louisa Ells, Phillippa Lally, Michael McEwan, Craig Melville, Leanne Harris, Olivia Wu

“…This included 16 RCTs, 2 controlled pre–post, 13 uncontrolled pre–post and 2 case-control studies. …”
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The Theoretical Approaches to Defining the Essence of the Concept of «National Security» and Its Components by Khaustova Viktoriia Ye., Trushkina Nataliia V.

“…The conception of the State policy for ensuring national security in Ukraine should be comprehensive, take into account both the impact and possible negative consequences of exogenous threats and hazards, contain strategic measures to determent or eliminate them, handle risks and protect critical infrastructure facilities. …”
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Impact of the COVID-19 pandemic on outcomes of acute ischemic stroke patients treated with endovascular therapy: A multicenter Canadian study. by Shenghua Zhu, Ammar Alam, Rebecca Thornhill, Vered Tsehmaister-Abitbul, Grant Stotts, Hailey Pettem, Richard Aviv, Ronit Agid, Aleksandra Pikula, Jai Jai Shiva Shankar, Genevieve Milot, Brian Van Adel, Samuel Yip, Manraj Heran, Robert Fahed, Fabio Settecase, Marlise P Dos Santos

“…Patients were more likely to have favourable outcomes (mRS at 90 days score of ≤ 2) post-EVT pre-pandemic than pandemic (53% vs 44%; p = 0.02). …”
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Network Pharmacology Approaches to Myocardial Infarction Reperfusion Injury: Exploring Mechanisms, Pathophysiology, and Novel Therapies by Joy Das, Ashok Kumar Sah, Ranjay Kumar Choudhary, Rabab H. Elshaikh, Utpal Bhui, Shreya Chowdhury, Anass M. Abbas, Manar G. Shalabi, Nadeem Ahmad Siddique, Raji Rubayyi Alshammari, Navjyot Trivedi, Khoula Salim Ali Buwaiqi, Said Al Ghenaimi, Pranav Kumar Prabhakar

“…The pathophysiology of MIRI involves complex, interrelated mechanisms including oxidative stress, calcium overload, mitochondrial dysfunction, inflammatory responses, apoptosis, and dysregulated autophagy. Post-reperfusion recovery is further complicated by structural and functional abnormalities such as microvascular obstruction, endothelial dysfunction, and myocardial stunning. …”
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Mitigation of chemotherapy-induced gut dysbiosis and diarrhea by supplementation with heat-killed Bacteroides fragilis by Xinwen Yan, Xinlong Lin, Jianhua Wu, Lijun Zheng, Yangyang Liu, Fang Wu, Ying Lin, Yishi Lu, Chongyang Huang, Binhai Shen, Hongbin Liu, Ruo Huang, Fengyi Hou, Qian Zhou, Mengyao Song, Ke Liu, Fangqing Zhu, Sheng Li, Yuqing Lin, Wei Wang, Ping Li, Wangjun Liao, Fachao Zhi

“…Fecal samples from eligible participants underwent shotgun metagenomic sequencing to comprehensively profile the gut microbiome composition and function. …”
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Cryopreserved leukapheresis enables scalable and distributed CAR-T manufacturing: a multi-platform comparative study by Menglin Ren, Xiaodie Wang, Jiangrui Chen, Yuanjian Xu, Yan Sun, Tao Liu, Qijun Qian, Zenghui Xu

“…Initial viability (91.0%) was lower than fresh samples (99.0%), but functional recovery post-electroporation and compatibility with both platforms were confirmed. …”
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AVATAR Virtual Reality Social therapy (AVATAR_VRSocial) for distressing voices and their interference in social everyday life in early psychosis: protocol of a single-blind paralle... by Tom K J Craig, Silvia Schneider, Philippa Garety, Thomas Ward, Mar Rus-Calafell, Melina Luker, Maren Marzinzik, Phuong-Mi Nguyen, Tobias Teismann, Nils Ehrbar, Ekincan Tas, Xiao Chi Zhang, Clementine Edwards, Mark Huckvale

“…Introduction Around 70% of people with psychosis experience auditory verbal hallucinations (AVHs), which can cause distress and impair the social functioning of the individual. AVATAR therapy works by facilitating a ‘face-to-face’ dialogue between the person and a digital representation (avatar) of their persecutory voice. …”
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Impact on Outcome of Different Mechanisms, Baseline Degree and Changes of Mitral Regurgitation in Patients With Aortic Stenosis Who Underwent Transcatheter Aortic Valve Replacement by Francesco Melillo, Annamaria Tavernese, Vincenzo Rizza, Francesco Putortì, Alberto Preda, Francesco Ancona, Giacomo Ingallina, Stefano Stella, Antonio Colombo, Marco Ancona, Alaide Chieffo, Nicola Buzzatti, Alessandro Castiglioni, Michele De Bonis, Francesco Maisano, Matteo Montorfano, Eustachio Agricola

“…Echocardiographic comprehensive MR assessment was performed at baseline and within 3 months post TAVR. …”
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Early detection to improve outcome in people with undiagnosed psoriatic arthritis: the PROMPT research programme including RCT by Neil McHugh, Laura Bojke, Mel Brooke Turfrey, Rachel Charlton, Myka Ransom, Laura C Coates, Howard Collier, Claire Davies, Emma Dures, Philip Helliwell, Jana James, Anya Lissina, Vishnu B Madhok, Alison L Nightingale, Jonathan Packham, Catherine Smith, Eldon Spackman, Cerys Tassinari, William Tillett, Sarah T Brown

“…The primary objective of TUDOR was to determine whether the early detection of undiagnosed PsA in people with psoriasis by ES compared to SC improves outcome in physical function at 24 months post registration. Secondary objectives were to compare disease and impact of disease between people diagnosed with PsA in the ES and SC arms at 24 months post registration. …”
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The TgAMPK-TgPFKII axis essentially regulates protein lactylation in the zoonotic parasite Toxoplasma gondii by Chenghuan Li, Yang Zhao, Qilong Li, Ran Chen, Ying Feng, Xiaoyu Sang, Xiangrui Li, Bang Shen, Ning Jiang, Qijun Chen

“…By elucidating the cellular localization and functional importance of TgPFKII, as well as its regulation through AMPK-specific inhibitors, we provide comprehensive insights into the metabolic and signaling networks that underpin T. gondii pathogenicity. …”
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Exploring the differential responses of ecosystem services to urbanization: A perspective from the metropolitan area as a whole and internal service bundles by Xianxin Meng, Zhenxiang Ling, Yingbiao Chen, Xiaohao Zheng, Xianghua Shi, Junyu Kuang, Wentao Chen, Yifan Yang, Zhifeng Wu, Zihao Zheng

“…This study employs ecological models, machine learning techniques, and post-hoc interpretive models to comprehensively analyze the spatiotemporal responses of ecosystem services to urbanization. …”
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Honeybees fed D-galactose exhibit aging signs with changes in gut microbiota and metabolism by Guanzhou Zhou, Jiabin Hu, Mengqi Xu, Yiyuan Li, Ruqi Chang, Jiaqi Zeng, Wanyue Dan, Lihua Peng, Zikai Wang, Gang Sun, Fei Pan, Yunsheng Yang

“…The CV group was fed sucrose solution; the DG group was fed D-galactose solution; and the SB group was fed D-galactose and sodium butyrate solution. A comprehensive assessment was conducted on day 15 post-treatment, including survival analysis, starvation test, motor, learning and memory ability tests, malondialdehyde test, and Smurf test. …”
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Single-cell transcriptome analysis revealed a suppressive tumor immune microenvironment in EGFR mutant lung adenocarcinoma by Lei Yang, Qing Zhou, Bo Zhang, Yi-Long Wu, Zhi-Hong Chen, Wen-zhao Zhong, Fen Wang, Yun-Ting He, Song Dong, Xue-Wu Wei, Wei-Dong Chen, Xiao-Rong Yang, Xue-Meng Shang

“…Being another important factor to affect the efficacy of immunotherapy, tumor microenvironment (TME) characteristics of this subgroup of NSCLC are not comprehensively understood up to date. Hence, we initiated this study to describe the specific TME of EGFR-mutant lung adenocarcinoma (LUAD) from cellular compositional and functional perspectives to better understand the immune landscape of this most common subtype of NSCLC.Methods We used single-cell transcriptome sequencing and multiplex immunohistochemistry to investigate the immune microenvironment of EGFR-mutant and EGFR wild-type LUADs and determined the efficacy of immunotherapy. …”
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