A Simplified Guide RNA Synthesis Protocol for SNAP- and Halo-Tag-Based RNA Editing Tools by Daniel Tobias Hofacker, Sebastian Kalkuhl, Jana Franziska Schmid, Shubhangi Singh, Thorsten Stafforst

“…This targeting is facilitated by short guide RNAs (gRNAs) complementary to the target transcript, which are chemically modified with benzylguanine or chloroalkane moieties to enable covalent binding to the respective self-labeling enzymes. …”
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Conceptual basis for the development of guidance for the use of biomarkers of effect in regulatory risk assessment of chemicals by European Food Safety Authority (EFSA), Antonio Hernández‐Jerez, Susanne Hougaard Bennekou, Laurentius (Ron) Hoogenboom, Henry Mcardle, Christina Pieper, Tanja Schwerdtle, Hendrik Van Loveren, Zainab Al Harraq, Cristina Croera, Anna Christodoulidou, Agnès De Sesmaisons, Chantra Eskes, Sara Levorato, Silvia Valtueña Martínez, Georgia Bompola, Lucian Farcal

“…In addition, relevant activities, initiatives and knowledge in this area were collected and analysed within a complementary mapping study. The outcome of this phase aimed to create a structured basis for future guidance, to identify challenges and to recommend a way forward for its development. …”
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Model Conceptual of Culture and Health-Seeking Behavior Among Mothers of Children with Stunting: A narrative review by Pipit Festi Wiliyanarti, Idham Choliq, Uswatun Hasanah

“…Mothers with limited health literacy may need help comprehending the nuances of nutrition and malnutrition in children. …”
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Vertex models capturing subcellular scales in epithelial tissues. by Zoë Lange, Franziska Matthäus, Mingfeng Qiu

“…Our review emphasizes the need for more comparative, systematic studies that identify specific classes of vertex models which share a set of well-defined properties, as well as a more in-depth discussion of modeling choices and their biological motivations.…”
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Diet and prey selectivity in co-occurring eelpout fish and bythograeid crabs in a deep-sea hydrothermal vent community by Deidric B. Davis, Nancy Smith

“…Our results emphasize the need to combine stomach contents, stable isotope analysis, and other complementary methodologies, to elucidate the role of predators in deep-sea food webs. …”
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Ginsenoside Rg3 Adjunctively Increases the Efficacy of Gefitinib Against NSCLC by Regulating EGFR Copy Number by Xinyi Lv, Yuehan Song, Tianhua Liu, Dingdan Zhang, Xinpeng Ye, Qingqing Wang, Rongrong Li, Jiayi Chen, Shujing Zhang, Xue Yu, Chunying Hou

“…In view of their low efficacy and susceptibility to drug resistance, there is an urgent need to find strategies to increase their efficacy and reduce the incidence of drug resistance. …”
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PARATEXTUALITY OF M. PRODANOVYCH`S NOVEL “COLLECTION”: AUTHOR`S CONCEPT, HISTORICAL AND CULTURAL CONTEXT by Nataliia L. Bilyk

“…They define the contours of the need for responsiveness and justice, and it turns out that the desire for good and humanity is potentiated.…”
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The effect of an educational intervention for university students on violence against the elderly using gamification: a non-randomized clinical trial by Juliana Ribeiro da Silva Vernasque, Miriam Fernanda Sanches Alarcon, Daiana Bonfim, Paula Sales Rodrigues, Eduardo Federighi Baisi Chagas, Maria José Sanches Marin

“…Conclusion: the gamified intervention was effective in stimulating protective attitudes and ethical perceptions of violence against the elderly, but there was no significant association with the knowledge variable. There is a need for more comprehensive studies that can provide complementary evidence to the results of this study.…”
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Legal Dogmatic Analysis on the Protection of Canines and Felines through the Use of Neutrosophic N-alectics by Jeannette Amparito Urrutia Guevara, Samantha Naomi Barreno Sailema, Niurka de los Ángeles Mejía Toscano, Jimmy Fernando Ramírez Coque

“…Animal welfare and animal rights protection laws are complementary yet antagonistic, and shortcomings exist irrespective of a partial imposition of biocentric ideals. …”
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A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications by Filippo Fronza, Roberto Verardo, Claudio Schneider

“…This protocol, by optimizing the buffer composition throughout the process, eliminates the need for buffer exchanges thereby reducing the risk of protein precipitation. …”
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Assessment of tannery effluents quality treated by electrocoagulation and ozonation: Physicochemical and ecotoxicological characterization. by Edwar Aguilar-Ascón, Liliana Marrufo-Saldaña, Julio Barra-Hinojosa, Robert Buleje-Del-Carpio

“…Despite significant pollutant removal, the results highlight that treated effluents may still present residual toxicity, emphasizing the need for complementary treatment strategies to achieve true environmental safety.…”
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Bioactive Plant Compounds as Alternatives Against Antifungal Resistance in the <i>Candida</i> Strains by Thainá dos Santos Dantas, Janaina Carla Barbosa Machado, Magda Rhayanny Assunção Ferreira, Luiz Alberto Lira Soares

“…This review underscores the potential of medicinal plants as complementary or alternative strategies to conventional antifungal therapies, emphasizing the need for multidisciplinary research to overcome these hurdles and harness the therapeutic potential of natural products.…”
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Emerging digital factual storytelling in English language learning: Investigating multimodal affordances by Wawan Gunawan, Silmy Arizatul Humaira, Tri Indri Hardini, Mahardhika Zifana

“…This study indicates the need to emphasize the purpose of text and to afford multimodal features to support the achievement of communication purposes.…”
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Advances in the Cognitive Management of Chronic Pain in Children through the Use of Virtual Reality Combined with Binaural Beats: A Pilot Study by Laia Riera, Sebastià Verger, Pedro J. Montoya, Francisco J. Perales

“…Chronic pain affects the quality of life of those affected. The need to investigate alternative and complementary methods to the pharmacological one to alleviate chronic pain is evident, so virtual reality and binaural tones have become a topic of interest in this field in recent years. …”
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Review on fish Polyculture practices: Sustainability, innovations and challenges by Dinesh Raj Pant, Jai Kumar

“…The benefits of polyculture  include increased fish production without the need for additional land and improved ecological and economic sustainability. …”
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Effects of oriental medicine music therapy in an ovarian cancer patient with So-Eum-type constitution: a case report by Seung-Hyun Lee, Eunhye Song, Seul-Ki Kim

“…The cancer incidence in Korea has been increasing, although there is a serious lack of supportive care for the treatment and management of the rapidly increasing number of cancer patients, and there is an immense need for therapeutic interventions to support cancer patients. …”
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Evaluating the spatiotemporal dynamics of agrometeorological droughts in semi-arid Gwayi agroecosystems (1990–2020) using multiple indices by Bright Chisadza, Onalenna Gwate, Simon Peter Musinguzi

“…Spatial analysis revealed a pronounced north-south drought gradient, with the southwestern region being particularly vulnerable, underscoring the need for targeted interventions.…”
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A Meta-Analysis of the Relationship Between Digital Maturity and Digital Transformation by Nurul Wahdaniyah, Achmad Nurmandi, Muhammad Younus

“…A key limitation lies in the absence of empirical validation, which underscores the need for future research using primary data and integrating complementary databases. …”
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Usefulness of Hemodynamic Sensors for Physiologic Cardiac Pacing in Heart Failure Patients by Eraldo Occhetta, Miriam Bortnik, Paolo Marino

“…Using sensors that have different attributes but that work in a complementary manners offers distinct advantages. However, complicated sensors interactions may occur. …”
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Essential Pieces of the Puzzle: The Roles of VEGF and Dopamine in Aging by Melanie B. Thompson, Sanjay P. Tirupattur, Nandini Vishwakarma, Laxmansa C. Katwa

“…This review describes existing evidence on the independent and potentially complementary roles of VEGF and dopamine in aging, emphasizing their influence on maintaining or improving neurovascular health. …”
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