Anticodon sequence determines the impact of mistranslating tRNAAla variants by Ecaterina Cozma, Megha Rao, Madison Dusick, Julie Genereaux, Ricard A. Rodriguez-Mias, Judit Villén, Christopher J. Brandl, Matthew D. Berg

“…Transfer RNAs (tRNAs) maintain translation fidelity through accurate charging by their cognate aminoacyl-tRNA synthetase and codon:anticodon base pairing with the mRNA at the ribosome. …”
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Patient-derived induced pluripotent stem cells to study non-canonical splicing variants associated with Hypertrophic Cardiomyopathy by Joanna Jager, Marta Ribeiro, Marta Furtado, Teresa Carvalho, Petros Syrris, Luis R. Lopes, Perry M. Elliott, Joaquim M.S. Cabral, Maria Carmo-Fonseca, Simão Teixeira da Rocha, Sandra Martins

“…However, conventional genetic tests primarily focus on the detection of exonic and canonical splice site variation. …”
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Molecular characteristics of hepatitis B virus among students and pregnant women in Chad by Nalda Debsikréo, Nafissatou Leye, Gora Lo, Maire Dehainsala, Odan Debsikréo, Ndeye Aminata Diaw, Diao Ba, Diabou Diagne, Abdoulaye Souare, Ndeye Dieynaba Diouf, N’da Kouamé Nazaire Kouadio, Isaac Darko Otchere, Ali Mahamat Moussa, Ndèye Coumba Toure-Kane, Françoise Lunel-Fabiani

“…The comparative mutational analysis identified low intragroup genetic diversity (0.004%) between Chadian strains with only two loci (codon positions 126 and 141) having nonsynonymous mutations including (Y126H, Y126N, Y126C, L140I, Y141S, Y141R, Y141H, Y141F). …”
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A single donor cassette enables site-specific knock-in at either the αAmy3 or αAmy8 locus in rice cells via CRISPR/Cas9 by Desyanti Saulina Sinaga, Po-Yu Huang, Chun‑Kai Huang, Chung-An Lu, Li-Fen Huang

“…Under the control of αAmy3-signal peptide, rcRBD proteins was detected in both the soluble cellular protein fraction and culture medium, whereas expression driven by the αAmy8 promoter-signal peptide was exclusively detected in the culture medium of rice suspension cells. …”
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Identification of a Novel Allele of TaCKX6a02 Associated with Grain Size, Filling Rate and Weight of Common Wheat. by Jie Lu, Cheng Chang, Hai-Ping Zhang, Sheng-Xing Wang, Genlou Sun, Shi-He Xiao, Chuan-Xi Ma

“…A novel 29-bp InDel behind the stop codon was detected by DNA sequence analysis between the two alleles of TaCKX6a02-D1. …”
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Two de novo UBR1 variants in trans as a cause of Johanson-Blizzard syndrome by Lukas Strych, Tomas Zavoral, Pavla Komrskova, Tomas Vanecek, Ivan Subrt

“…The inclusion of 70 bp of the intronic sequence generated a frameshift and a premature termination codon leading to nonsense-mediated decay, as detected by qPCR. …”
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First report and evidence of multiple origins of diflubenzuron resistance alleles in Culex pipiens mosquito from Cyprus by Valentina Mastrantonio, Marlen Vasquez, Gregoris Notarides, Eleni Patsoula, Valentina Lucchesi, Flavio Piras, Romeo Bellini, Daniele Porretta

“…Results Our screening revealed the occurrence of the I1043F allele in all the districts analyzed. Notably, a new gene codon underlying the I1043F allele was detected. …”
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First two mitochondrial genomes for the order Filobasidiales reveal novel gene rearrangements and intron dynamics of Tremellomycetes by Qiang Li, Zhijie Bao, Ke Tang, Huiyu Feng, Wenying Tu, Lijiao Li, Yunlei Han, Mei Cao, Changsong Zhao

“…Comparative mitogenomic analysis revealed that the genetic contents, tRNAs, and codon usages of the two Filobasidium species differed greatly. …”
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Comparative Mitogenomes and Phylogenetic Analyses of Coccinellidae (Coleoptera: Coccinelloidea) by Qiaoqiao Liu, Pingzhou Zhu, Shiwen Xu, Chunyan Yang, Fan Song, Yufang Meng, Jinhong Zhou, Hailin Yang, Weidong Huang

“…The variable number tandem repeat (VNTR) was detected in the LNCR of Calvia chinensis and Oenopia kirbyi, indicating that the LNCR may be associated with the transcriptional regulation of the mitogenome. …”
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Evaluation of the morpho-physiological traits and the genetic diversity of durum wheat’s salt tolerance induced by silver nanoparticles by Sara B.H. Awaly, Neama H. Osman, Hend M. Farag, Ibrahim H. Yacoub, Mohamed Mahmoud-Aly, Nagwa I. Elarabi Elarabi, Dalia S. Ahmed

“…Likewise, the impact of AgNPs on the higher plants was significant at P ≤ 0.05. Thirty-seven start codon-targeted (SCoT) primers and forty-two sequences related amplified polymorphism (SRAP) primers were employed to assess the genetic influence of AgNPs on wheat cultivars. …”
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Gene expression study of durum wheat hybrids under water deficit conditions by Ahmed Mohsen, Khaled Adly Khaled, Ayman Hamdi Mahdi, Aladdin Hamwieh, Sherif El-Areed

“…The study aimed to assess the tolerance of six durum wheat cultivars to water deficit stress, develop new tolerant durum wheat hybrids, and detect gene expressions to tolerance water deficit stress, using start codon-targeted (SCoT) markers. …”
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De Novo Assembly and Comparative Analysis of the Mitochondrial Genomes for Six <i>Rubus</i> Species by Yujie Shi, Zhen Chen, Jingyong Jiang, Qianfan Li, Wei Zeng

“…These six species exhibit similar codon usage patterns. A large number of gene transfers were detected between organellar genomes of six <i>Rubus</i> species. …”
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Genetic variants of hepatitis B virus in patients with chronic hepatitis B by E. A. Elpaeva, O. E. Nikitina, M. M. Pisareva, I. V. Shilova, V. A. Greshnjakova, M. P. Grudinin, O. I. Kiselev

“…The substitution rtA181T in three patients resulted in formation of stop codon (sW172*) and premature termination of surface protein synthesis. …”
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Chloroplast genome features and intraspecific variation of Rosa praelucens by WANG Qigang1, CAO Shirui1,2, WANG Huichun1, MA Changle2, YAN Huijun1, QIU Xianqin1, JING Weikun1, JIAN Hongying1*

“…The genomes encoded 132 function genes, mainly related with photosynthesis and self-replication. A total of 27 155 codons, preferring using codon ending of A or U, were found in all the coding sequences.(2)A total of 36 repeats and 73 simple sequence repeats(SSRs)were detected in the chloroplast genome of R. praelucens. …”
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Different results despite high homology: Comparative expression of human and murine DNase1 in Pichia pastoris. by Jan-Ole Krischek, Hans Georg Mannherz, Markus Napirei

“…In addition to an impaired cleavage of the αMF pro-peptide, which we also detected previously for mDNase1, expression of hDNASE1 resulted in a 70-80 times lower yield although both orthologues share a high structural and functional homology. …”
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Comparative Genomics and Phylogenetic Analyses of Aquarius macrophyllus and Related Genera in Alismataceae Based on Plastome Data by Jie Li, Shengnan Wei, Jianan Ying, Qixiang Lu

“…Two sequence inversions of 5.6 and 6.4 kb were detected in Sagittaria and Caldesia, respectively. …”
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Cloning and identification of human colipase-like 3 and its homologous cDNAs by LU Yan, XUE Yong-chang, YU Peng, WANG Ping-zhang

“…Only hCLPSL3-v1 contained a complete open reading frame (ORF), encoding 159 amino acids, whereas the ORF of hCLPSL3-v2 was interrupted by PTC (premature stop codon) and it possibly was a substrate of nonsense-mediated mRNA decay. …”
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Current Genetic Models for Prediction of Primary Myelofibrosis by LB Polushkina, VA Shuvaev, MS Fominykh, YuA Krivolapov, EA Belyakova, ZP Asaulenko, V Motyko, LS Martynenko, MP Bakai, NYu Tsybakova, SV Voloshin, SS Bessmeltsev, AV Chechetkin, IS Martynkevich

“…JAK2, CALR, MPL mutations were detected in 55 (50 %), 28 (25.5 %), and 7 (6.4 %) out of 110 patients, respectively. …”
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Assembly and Comparative Analysis of the Complete Mitochondrial Genome of <i>Hippophae salicifolia</i> by Zhefei Zeng, Chunmin Mao, Zhuo Shang, Ngawang Norbu, Ngawang Bonjor, Xiaoyan Jia, Wei Li, Wenju Zhang, Junwei Wang, La Qiong

“…We identified extensive repetitive elements, including 188 SSRs, 20 tandem repeats, and 455 dispersed repeats, and explored their potential roles in genome evolution. Codon usage analysis showed a bias for codons ending in A or U, while RNA editing analysis revealed 415 sites that mostly convert hydrophilic to hydrophobic amino acids. …”
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Molecular surveillance of artemisinin resistance-linked PFK13 gene polymorphisms in Adamawa State, Nigeria by Mahmoud Suleiman Jada, Yusuf Umar, Aliyu Abdullahi Pela, Auwal Adamu, Hauwa Ahmed Zailani, Abdullahi Usman Wurochekke

“…Sequencing and sequence analysis were conducted to check for mutations at validated codon positions.   Results: Out of the 240 samples collected, RDT revealed 100% to be positive for P. falciparum while microscopy confirmed P. falciparum presence in 214 samples (89.17%). …”
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