Multiple spacer sequence typing of Coxiella burnetii carried by ticks in Gansu, China by Ze-Yun Xu, Fang-Ni Wang, Rui Jian, Jing Xue, Ya-Chun Guo, Wen-Ping Guo

“…The 16S rRNA gene analysis showed that the novel C. burnetii variants detected in this study were closely related to other C. burnetii strains in the world. …”
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A novel monoclonal antibody differentiates highly pathogenic serotype 4 fowl adenovirus through targeting 188R in the Hexon by Ye Tang, Bai Xie, Shi Liu, Quan Xie, Weikang Wang, Junpeng Chen, Xiaoyan Tian, Junji Gan, Tuofan Li, Shengnan Wang, Zhimin Wan, Hongxia Shao, Aijian Qin, Jianqiang Ye

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PolyA polymorphisms in 3' flanking region of pig neuronal protein 3.1 gene and their relationships with meat traits by Yang Yun, Zeng Yongqing, Zhang Zhe, Xu Zhenggang, Chen Wei, Fang Guofeng, Wang Shoudong

“…The P311 is extensively distributed in the nervous and muscular systems and had the functions in myofibroblast differentiation and alveolar generation processes. It is also closely related to the fat deposition. The 3' flanking region doesn't code for protein, but it can affect the phenotype by some ways, such as the regulation of the degradation and translation rates of the mRNA. …”
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MMTSCNet: Multimodal Tree Species Classification Network for Classification of Multi-Source, Single-Tree LiDAR Point Clouds by Jan Richard Vahrenhold, Melanie Brandmeier, Markus Sebastian Müller

“…We propose a fully Multimodal Tree Species Classification Network (MMTSCNet) that processes Light Detection and Ranging (LiDAR) point clouds, Full-Waveform (FWF) data, derived features, and bidirectional, color-coded depth images in their native data formats without any modality transformation. …”
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Identification of pandemic ST147, ESBL-type β-lactamases, carbapenemases, and virulence factors in Klebsiella pneumoniae isolated from southern Peru by Elsa Gladys Aguilar-Ancori, Marishani Marin-Carrasco, Laura Isabel Campo-Pfuyo, Julia Griselda Muñiz-Duran, Abraham Espinoza-Culupú

“…Protease activity was detected in 19.8% of the strains, and lipase activity in 1.1%. …”
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LncRNA of peripheral blood mononuclear cells: HYMAI acts as a potential diagnostic and therapeutic biomarker for female major depressive disorder by Tianyi Bu, Jiarun Yang, Jiawei Zhou, Yeran Liu, Kexin Qiao, Yan Wang, Jili Zhang, Erying Zhao, Boakye Kwame Owura, Xiaohui Qiu, Zhengxue Qiao, Yanjie Yang

“…Meanwhile, a new perspective for future female MDD prevention, diagnosis and treatment, evaluation, detection, and intervention is provided.…”
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Narva noorte väärtused ja identiteet by Andu Rämmer, Maria Žuravljova, Anne Kivimäe, Maria Anderson

“…A social representation is a system of values, ideas and practices with a twofold function: first, to enable individuals to orient themselves in the everyday world and to master it; and second, to provide codes for social exchange or naming and classifying unambiguously the various aspects of their world and their individual group history. …”
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Strain-level genomic analysis of serotype, genotype and virulence gene composition of group B streptococcus by Zhen Zeng, Meng Li, Simin Zhu, Ke Zhang, Yifan Wu, Minzi Zheng, Yang Cao, Zhenyu Huang, Qinping Liao, Lei Zhang, Lei Zhang

“…However, the composition of virulence genes was more closely related to the phylogeny of GBS, among which simultaneous presence of Srr2 and HygA exhibit significant association with hypervirulence. …”
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Amugulang virus, a novel hantavirus harboured by small rodents in Hulunbuir, China by Xiaohu Han, Lianhong Zhang, Mingxuan Zhang, Qing Xin, Yongxiang Zhao, Ya Wen, Hua Deng, Jinguo Zhu, Qin Dai, Mei Han, Tianyu Yang, Saiji Lahu, Feng Jiang, Zeliang Chen

“…Metagenomic analysis identified three complete nucleic acid sequences from the S, M, and L segments of the Hantaviridae family, which were closely related to the Khabarovsk virus. The nucleotide coding sequences for S, M, and L (1392 nt, 3465 nt, and 6491 nt, respectively) exhibited similarities of 82.34%, 81.68%, and 81.94% to known sequences, respectively, while protein-level analysis indicated higher similarities of 94.92%, 94.41%, and 95.87%, respectively. …”
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Mitochondrial genome of Lonicera macranthoides: features, RNA editing, and insights into male sterility by Zhong Chen, Wei Zhuo, Yuqi Wang, Junpeng Qi, Li Liu, Sheng’E. Lu, Han Wang, Tao Sun, Liqiang Wang, Fengming Ren, Fengming Ren

“…They are semi-autonomous, maternally inherited, and closely linked to cytoplasmic male sterility (CMS) in plants. …”
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Comparative analysis of 18 chloroplast genomes reveals genomic diversity and evolutionary dynamics in subtribe Malaxidinae (Orchidaceae) by Lin Li, Wenting Yang, Qiang Liu, Xingyou Jiang, Kunlin Wu, Lin Fang, Mingzhi Li, Songjun Zeng, Shijin Li

“…Each genome contains 123–133 genes, including 74–86 protein-coding genes, 38 tRNA genes, 8 rRNA genes and 1–8 pseudogenes. …”
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Expression characteristics and transcriptional regulation analysis of VlCKX5 gene in grape by LIU Yiting, WANG Ruxin, ZHANG Haimeng, JING Pengwei, SHI Qiaofang, ZHAO Xiaochun, YU Yihe

“…Subsequently, the VlAGL6a gene was cloned into the pGADT7 vector to create the recombinant prey plasmid pGADT7-VlAGL6a, which was transfected into a yeast strain positive for the bait genome to perform yeast one-hybrid (Y1H) interaction detection. …”
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Modular and efficient assembly of CRISPR vectors and construction of an apple gene editing efficiency evaluation system by HOU Feifan, HUANG Chuanmi, HAO Pengbo, CONG Liu, BAI Tuanhui, SHI Jiangli, WANG Miaomiao, LIU Yu, ZHAO Yujie, WAN Ran, SHEN Yawen, ZHANG Hengtao, ZHANG Kunxi, ZHENG Xianbo, JIAO Jian

“…Conventional plasmids were converted into modified vectors compatible with the Golden Gate cloning system respectively. Subsequently, the e35S promoter, SpyCas9 coding sequence and NOS terminator were amplified by PCR and seamlessly assembled into pUC19-Esp3 Ⅰ-Bb vector to construct expression module A (e35S-Cas9-NOS); For targeted editing of apple PDS (Phytoene desaturase) gene, the specific gRNA (guide RNA) sequence was designed and constructed expression module B (AtU6-gRNA) through PCR amplification using primers containing Bsa Ⅰ restriction enzyme site; Based on the same strategy, the RUBY reporter element was amplified by primers with additional Bsa Ⅰ restriction enzyme site to form expression module C. …”
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Approaches used to prevent and reduce the use of restrictive practices on adults with learning disabilities: a realist review by Joy Duxbury, Alina Haines-Delmont, John Baker, Peter Baker, Gary Bourlet, Elaine Craig, James Ridley, Rachel Whyte, Beth Morrison, Michaela Thomson, Anthony Tsang, Tella Lantta

“…(How CQC Identifies and Responds to Closed Cultures. 2022. URL: How CQC identifies and responds to closed cultures – Care Quality Commission). …”
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A xyloglucan endotransglucosylase/hydrolase gene, IbXTH16, increases cold tolerance in transgenic sweetpotato by Tao Yu, Jiaquan Pan, Sitong Liu, Zitong Yang, Zhenlei Liu

“…In-depth studies on the gene functions underlying cold resistance are important for genetic engineering in sweetpotato.MethodsThe IbXTH16 gene was cloned using a homologous cloning approach. Its expression was detected in sweetpotato leaves subjected to low-temperature stress and brassinosteroid treatment. …”
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Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinus L.) by Ritesh Ghosh, Bosung Choi, Byoung-Kwan Cho, Hyoun-Sub Lim, Sang-Un Park, Hyeun-Jong Bae, Savithiry Natarajan, Hanhong Bae

“…Cinnamoyl-CoA reductase (CCR) is an important enzyme for lignin biosynthesis as it catalyzes the first specific committed step in monolignol biosynthesis. We have cloned a full length coding sequence of CCR from kenaf (Hibiscus cannabinus L.), which contains a 1,020-bp open reading frame (ORF), encoding 339 amino acids of 37.37 kDa, with an isoelectric point (pI) of 6.27 (JX524276, HcCCR2). …”
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HIV chromatin is a preferred target for drugs that bind in the DNA minor groove. by Clayton K Collings, Donald W Little, Samuel J Schafer, John N Anderson

“…The results demonstrated that the MGBDs inhibited the assembly of nucleosomes onto all of the HIV-1 segments in a manner that was proportional to the A-bias, but had no detectable effect on the formation of nucleosomes on control cloned fragments or genomic DNA from chicken and human. …”
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Regulatory puzzle of xyn1 gene (xylanase1) expression in Trichoderma reesei by Robert L Mach, Elisabeth Würleitner, Astrid R Stricker, Roman Rauscher, Christian Wacenovsky

“…The inverted repeat of GGCTAA closely resembles the consensus sequences of the cellulase and xylanase regulators Ace1, Ace2 and, Xyr1 (encoded by xyr1, cloned and characterised in this study) EMSA with heterologously expressed components of each factor and of the T. reesei Hap2/3/5 protein complex revealed that the basal transcriptional complex is formed by Xyr1 and the Hap2/3/5. …”
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The role of interruptions in polyQ in the pathology of SCA1. by Rajesh P Menon, Suran Nethisinghe, Serena Faggiano, Tommaso Vannocci, Human Rezaei, Sally Pemble, Mary G Sweeney, Nicholas W Wood, Mary B Davis, Annalisa Pastore, Paola Giunti

“…We have carried out an extensive study in which we have complemented DNA sequence determination with cellular and biophysical models. By sequencing cloned normal and expanded SCA1 alleles taken from our cohort of ataxia patients we have determined sequence variations not detected by allele sizing and observed for the first time that repeat instability can occur even in the presence of CAG interruptions. …”
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The complete mitochondrial genome of Epicauta sibirica Pallas, 1773 (Coleoptera: Meloidae) by Nan Yang, Suhua Zhang, Nashen Jin, Ning Wang, Zilong He, Yufang Xu, Kejian Lin

“…The genus Epicauta was detected as a monophyletic group and more closely related to the genus Lytta than other genera within Meloidae. …”
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