Expression of Acc-royalisin from royal jelly of Apis cerana cerana in Escherichia coli and preparation of its polyclonal antibody by DING Mei-hui, JIN Feng, SHEN Li-rong, CHEN Zheng-xian

“…The coding region of pre-pro-Acc-royalisin was amplified by PCR from cDNA library of the Chinese honeybee, Apis cerana cerana head, and was cloned into the vector pGEX-4T-2 for expression in Escherichia coli BL21. …”
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A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells by Xiaobing Han, Liangxin Chen, Peng Sun, Xiuqing Wang, Qian Zhao, Lingfeng Liao, Dejin Lou, Nan Zhou, Yujun Wang

“…Abstract The advance of high-throughput sequencing enhances the discovery of short ORFs embedded in long non-coding RNAs (lncRNAs). Here, we uncovered the production and biological activity of lncRNA-hidden polypeptides in lung adenocarcinoma (LUAD). …”
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Genome-wide proximity between RNA polymerase and DNA topoisomerase I supports transcription in Streptococcus pneumoniae. by María-José Ferrándiz, Pablo Hernández, Adela G de la Campa

“…Both enzymes occupied a narrow region close to the ATG codon. In addition, RNAP displayed a regular distribution throughout the coding regions. …”
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Nine complete chloroplast genomes of the Camellia genus provide insights into evolutionary relationships and species differentiation by Yanfei Cai, Min Tian, Yingjie Yang, Ziming Shi, Peifei Zhao, Jihua Wang

“…They consistently predicted 87 protein-coding genes, although variations were observed in the rRNA and tRNA genes. …”
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Plastome and transcriptome analysis reveals a degenerated photosynthesis-related pathway in the mycoheterotrophic vanilloid orchid Cyrtosia lindleyana by Shiou Yih Lee, Peishan Zou, Zhihui Chen, Kenji Suetsugu, Wenbo Liao

“…Results The plastome, despite its reduction to 89,414 base pairs, maintained its quadripartite structure. We detected 63 potentially intact genes, of which nine protein-coding genes exhibited predicted RNA-editing sites. …”
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First two mitochondrial genomes for the order Filobasidiales reveal novel gene rearrangements and intron dynamics of Tremellomycetes by Qiang Li, Zhijie Bao, Ke Tang, Huiyu Feng, Wenying Tu, Lijiao Li, Yunlei Han, Mei Cao, Changsong Zhao

“…Positive selection was observed in several protein-coding genes (PCGs) in the Agaricomycotina, Pucciniomycotina, and Ustilaginomycotina species, including cob, cox2, nad2, and rps3 genes. …”
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Mitochondrial genome analysis of the endangered Oreocharis esquirolii: insights into evolutionary adaptation and conservation by Lang Chen, Rong-Rong Yan, Chong-Yi Yang, Li-Zhen Ling, Xin-Xiang Bai, Qi-Fei Ren, Guo-Xiong Hu

“…Positive selection signals were detected in seven coding genes of the O. esquirolii mitogenome, with a potential impact on environmental adaptation. …”
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Investigation of growth traits in Turkish Merino lambs using multi-locus GWAS approaches: Karacabey Merino by Yalçın Yaman, Ramazan Aymaz, Murat Keleş, Yiğit Emir Kişi, Ecem Hatipoğlu, Arzu Özdemir, Elif Çetinkaya

“…Gene annotation revealed most associated SNPs were within or very close to protein-coding genes, suggesting a functional role in trait influence. …”
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Feasibility of decentralised deployment of Xpert MTB/RIF test at lower level of health system in India. by Neeraj Raizada, K S Sachdeva, Achuthan Sreenivas, Bhavin Vadera, R S Gupta, Malik Parmar, Shubhangi Kulsange, Ameet Babre, Rahul Thakur, Christen Gray, Ranjani Ramachandran, Umesh Alavadi, Mayank Ghedia, Balasangameshwara Vollepore, Puneet Dewan, Catharina Boehme, C N Paramsivan

“…<h4>Background</h4>Xpert MTB/RIF is an automated cartridge-based nucleic acid amplification test that has demonstrated its potential to detect tuberculosis and rifampicin resistance with high accuracy. …”
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Genetic Analysis of 'PAX6-Negative' Individuals with Aniridia or Gillespie Syndrome. by Morad Ansari, Jacqueline Rainger, Isabel M Hanson, Kathleen A Williamson, Freddie Sharkey, Louise Harewood, Angela Sandilands, Jill Clayton-Smith, Helene Dollfus, Pierre Bitoun, Francoise Meire, Judy Fantes, Brunella Franco, Birgit Lorenz, David S Taylor, Fiona Stewart, Colin E Willoughby, Meriel McEntagart, Peng Tee Khaw, Carol Clericuzio, Lionel Van Maldergem, Denise Williams, Ruth Newbury-Ecob, Elias I Traboulsi, Eduardo D Silva, Mukhlis M Madlom, David R Goudie, Brian W Fleck, Dagmar Wieczorek, Juergen Kohlhase, Alice D McTrusty, Carol Gardiner, Christopher Yale, Anthony T Moore, Isabelle Russell-Eggitt, Lily Islam, Melissa Lees, Philip L Beales, Stephen J Tuft, Juan B Solano, Miranda Splitt, Jens Michael Hertz, Trine E Prescott, Deborah J Shears, Ken K Nischal, Martine Doco-Fenzy, Fabienne Prieur, I Karen Temple, Katherine L Lachlan, Giuseppe Damante, Danny A Morrison, Veronica van Heyningen, David R FitzPatrick

“…Sequence analysis of the FOXC1 and PITX2 coding regions identified two plausibly pathogenic de novo FOXC1 missense mutations (p.Pro79Thr and p.Leu101Pro). …”
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Characterize the Complete Mitogenome of Semiaquilegia guangxiensis and Assess the Efficiency of the Mitochondrial Genes in Ranunculales Phylogeny by Zheng‐Juan Zhu, Xin‐Mei Qin, Peng‐Wei Li, Yong‐Bin Lu, Xiao‐Yuan Mo, Yuan Fang, Qiang Zhang

“…The results showed that the mitogenome was composed of two independent molecules, which had a total length of 522,981 bp, a GC content of 45.69%, and 58 genes including 34 protein‐coding genes (PCGs), 21 tRNA genes, and three rRNA genes. …”
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Preparation and application of polyclonal antibody against a cysteine-rich protein encoded by Chinese wheat mosaic virus by DAI Yuanxing, GUO Liuming, HE Jing, SHEN Zhengrong, GENG Yanfei, LÜ Mingfang, YUAN Zhengjie, LI Jing, ZHANG Hengmu

“…To further study CRP functions and CWMV infection mechanisms, the CRP coding region was amplified by reverse transcription-polymerase chain reaction (RT-PCR) from leaves of CWMV-infected wheat and cloned into the prokaryotic expression vector pET-32a. …”
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Pervasive within-host recombination and epistasis as major determinants of the molecular evolution of the foot-and-mouth disease virus capsid. by Luca Ferretti, Eva Pérez-Martín, Fuquan Zhang, François Maree, Lin-Mari de Klerk-Lorist, Louis van Schalkwykc, Nicholas D Juleff, Bryan Charleston, Paolo Ribeca

“…This population structure enabled the detection of extensive within-host recombination in the genomic region coding for structural proteins and allowed recombination rates between the two sub-populations to be estimated. …”
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Emerging roles of exosomal circRNAs in non-small cell lung cancer by Hongyuan Yin, Jiayi Shi, Shaoling Li, Qianhui You, Huici Zhu, Chinying Koo, Baonian Liu, Likun Hou, Chunyan Wu

“…Circular RNA (circRNA), a type of non-coding RNA possessing a covalently closed loop structure, substantial abundance, and tissue-specific expression patterns, is stably enriched in exosomes. …”
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Assembly and Analysis of the Mitochondrial Genome of <i>Hippophae rhamnoides</i> subsp. <i>sinensis</i>, an Important Ecological and Economic Forest Tree Species in China by Jie Li, Song-Song Lu, Yang Bi, Yu-Mei Jiang, Li-Dan Feng, Jing He

“…Phylogenetically, the Rosales order showed close relationships with Fagales, Fabales, Malpighiales, and Celastrales. …”
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Population genetics reveal potential threats from low maternal genetic diversity in wild Asian elephants in China by Minhui Shi, Yinping Tian, Yongjing Tang, Haimeng Li, Jishan Wang, Yue Ma, Xin Liu, Ahimsa Campos-Arceiz, Fei Chen, Tianming Lan

“…In addition, we extracted approximately 600 kb of non-coding genomic regions for a comparative analysis of the genetic structure between the nuclear and mitochondrial DNA. …”
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Aquificae overcomes competition by archaeal thermophiles, and crowding by bacterial mesophiles, to dominate the boiling vent-water of a Trans-Himalayan sulfur-borax spring. by Nibendu Mondal, Subhajit Dutta, Sumit Chatterjee, Jagannath Sarkar, Mahamadul Mondal, Chayan Roy, Ranadhir Chakraborty, Wriddhiman Ghosh

“…Among these 66, Fervidobacterium, Halomonas, Hydrogenobacter, Paracoccus, Sulfurihydrogenibium, Tepidimonas, Thermus and Thiofaba (or their close phylogenomic relatives) were presently detected as metagenome-assembled genomes (MAGs). …”
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Adaptive neuro-fuzzy inference systems for improved mastitis classification and diagnosis by Javad Shirani Shamsabadi, Saeid Ansari Mahyari, Mostafa Ghaderi-Zefrehei

“…However, due to the interplay of multiple evaluation criteria and the closely clustered fitted values, determining a definitive best model almost remained challenging In addition to improving udder health, milk quality, and economic viability, this research can contribute to ongoing soft computing efforts to improve mastitis detection and management in dairy cattle. …”
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Etiologic Characteristics of Influenza Epidemics on the Territory of Russia in the Period of Pandemic COVID-­19 in 2020–2023 by P. A. Petrova, N. I. Konovalova, A. Y. Boyarintseva, D. M. Danilenko, A. D. Vasilieva, T. N. Shelepanova, A. V. Prokopets, M. Y. Eropkin

“…The strains under study were very close to the strain A/Darwin/09/21, recommended by the WHO as the vaccine strain for the season 2022-2023. …”
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Species-specific RNA barcoding technology for rapid and accurate identification of four types of influenza virus by Shuai Jiang, Yunyun Ding, Gaili Zhao, Shunxing Ye, Shucan Liu, Yan Yin, Zeqi Li, Xiaoxiao Zou, Daolong Xie, Changqiao You, Xinhong Guo

“…Ultimately, all four types of IVs were visualized in a combination of one-dimensional and two-dimensional codes and stored in an online database named Influenza Virus Barcode Database (FluBarDB, http://virusbarcodedatabase.top/database/index.html ). …”
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