MPicker: visualizing and picking membrane proteins for cryo-electron tomography by Xiaofeng Yan, Shudong Li, Weilin Huang, Hao Wang, Tianfang Zhao, Mingtao Huang, Niyun Zhou, Yuan Shen, Xueming Li

“…Abstract Advancements in cryo-electron tomography (cryoET) allow the structure of macromolecules to be determined in situ, which is crucial for studying membrane protein structures and their interactions in the cellular environment. However, membranes are often highly curved and have a strong contrast in cryoET tomograms, which masks the signals from membrane proteins. …”
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Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology by Casper Berger, Helena Watson, James H. Naismith, Maud Dumoux, Michael Grange

“…Abstract Cryo focused ion beam lamella preparation is a potent tool for in situ structural biology, enabling the study of macromolecules in their native cellular environments. However, throughput is currently limited, especially for thicker, more biologically complex samples. …”
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Stochastic gene expression in auxin signaling in the floral meristem of Arabidopsis thaliana by Shuyao Kong, Byron Rusnak, Mingyuan Zhu, Adrienne H. K. Roeder

“…Finally, we propose spatial averaging as a mechanism that buffers cellular gene expression noise, allowing the formation of robust global expression patterns. …”
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Spatial heterogeneity and peptide availability determine CTL killing efficiency in vivo. by Thea Hogan, Ulrich Kadolsky, Sim Tung, Benedict Seddon, Andrew Yates

“…Our analyses demonstrate how population-averaged parameters in models of immune responses can be dissected to account for both spatial and cellular heterogeneity.…”
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Polysaccharide Hydrogels Support the Long-Term Viability of Encapsulated Human Mesenchymal Stem Cells and Their Ability to Secrete Immunomodulatory Factors by Fahd Hached, Claire Vinatier, Pierre-Gabriel Pinta, Philippe Hulin, Catherine Le Visage, Pierre Weiss, Jérôme Guicheux, Aurélie Billon-Chabaud, Gaël Grimandi

“…Human adipose-derived MSCs (hASC) were encapsulated either in alginate or in Si-HPMC, and the cellularized particles were cultured for up to 1 month. …”
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pytom-match-pick: A tophat-transform constraint for automated classification in template matching by Marten L. Chaillet, Sander Roet, Remco C. Veltkamp, Friedrich Förster

“…We benchmarked pytom-match-pick on public datasets, demonstrating improved classification and localization of macromolecules like ribosomal subunits and proteasomes that led to fewer artifacts in subtomogram averages. This tool promises to advance visual proteomics by improving the efficiency and accuracy of macromolecule detection in cellular contexts.…”
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Mapping grey and white matter activity in the human brain with isotropic ADC-fMRI by Arthur P. C. Spencer, Jasmine Nguyen-Duc, Inès de Riedmatten, Filip Szczepankiewicz, Ileana O. Jelescu

“…Apparent diffusion coefficient (ADC) offers an alternative fMRI contrast sensitive to transient cellular deformations during neural activity, facilitating detection of both grey and white matter activity. …”
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Standards: Exposure Limits for Brief High Intensity Pulses of Radiofrequency Energy Between 6 and 300 GHz by Kenneth R. Foster, Ilkka Laakso, Quirino Balzano

“…This Standards paper considers limits of the International Commission on Nonionizing Radiation (ICNIRP) and Institute of Electrical and Electronics Engineers (IEEE C95.1-2019) on “brief exposures” to radiofrequency energy (RF-EMF), that vary significantly within the 6 minute averaging time specified in the limits. This situation arises in two important contexts: exposures from cellular base stations employing Multiple Input Multiple Output (MIMO) antennas with beamforming that sweep beams around the service area, and exposure to pulsed RF-EMF with high peak power but low duty cycle. …”
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Peripubertal DEHP exposure impairs reproductive function in adult male mice by inducing damage to Sertoli cells and disrupting the blood-testis barrier by XU Guiyong, XU Guiyong, ZHANG Lu, ZHANG Lu, SUN Fengqiong, SUN Fengqiong

“…In the 25, 125, 250 and 500 mg/kg DEHP exposure groups, the thickness of the seminiferous epithelium was obviously decreased, with more cellular vacuolization and notable epithelial atrophy and degeneration. …”
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HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION (HPAEC–PAD) AND PHYSICOCHEMICAL ANALYSIS OF HONEY: EVALUATION OF THE COMPOSITION AND THE QUALITY by MABROUKA BOUACHA, SANA BESNACI, MINA KOUCH, AKILA ABDI, PAUL SCHWEITZER

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One‐shot 13C15N‐metabolic flux analysis for simultaneous quantification of carbon and nitrogen flux by Khushboo Borah Slater, Martin Beyß, Ye Xu, Jim Barber, Catia Costa, Jane Newcombe, Axel Theorell, Melanie J Bailey, Dany J V Beste, Johnjoe McFadden, Katharina Nöh

“…Abstract Metabolic flux is the final output of cellular regulation and has been extensively studied for carbon but much less is known about nitrogen, which is another important building block for living organisms. …”
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Exploring Imaging Applications of a Red-Emitting π-Acceptor (π-A) Pyrene-Benzothiazolium Dye by Chathura S. Abeywickrama, Enya Huang, Wenhui Yan, Michael A. Vrionides, Paaramitha Warushavithana, Kristen A. Johnson, Robert V. Stahelin, Yi Pang, Tomoyasu Mani, Kaveesha J. Wijesinghe

“…The optical properties of BTP are studied in intracellular environments through advanced fluorescence microscopy techniques, with BTP exhibiting a noticeable shift toward blue (λ_em ≈ 590 nm) emissions in cellular lysosomes. The averaged photon arrival time (AAT)-based studies exhibit two different emissive populations of photons, indicating the probe’s dynamic equilibrium between two distinctively different lysosomal microenvironments. …”
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GluA1 Phosphorylation Alters Evoked Firing Pattern In Vivo by Balázs Barkóczi, Gábor Juhász, Robert G. Averkin, Imre Vörös, Petra Vertes, Botond Penke, Viktor Szegedi

“…Phosphorylation of GluA1 regulates channel properties and trafficking. The firing rate averaged over several hundred ms is used to monitor cellular input. …”
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Expanding our understanding of (mal)adapted stress physiology in psychiatric disorders: achieving single-cell characterisation of steroids and neuropeptides by Katrina Z. Edmond, Natalie Matosin

“…The emergence of single-cell and spatial technologies now offers unprecedented insight into the precise cellular, molecular and spatial context in which neuroactive steroid and neuropeptide signalling occurs. …”
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Statistical data analysis methods in Brillouin spectroscopy: Tutorial by Christopher G. Poulton, Hadi Mahmodi, Matthew D. Arnold, Luke McAlary, Lezanne Ooi, Irina Kabakova

“…Since the majority of subcellular and extracellular matrix components are significantly smaller than 1 μm, many cell and tissue components become averaged in a single imaging voxel. This, in turn, presents a challenge for data analysis of Brillouin microscopy measurements collected from heterogeneous samples, especially since the cellular components often have similar and closely positioned spectral features. …”
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Analysis of Satellite and In Situ Optical Proxies for PIC and POC During GEOTRACES GP15 and GP17‐OCE Transects From the Subarctic North Pacific to the Southern Ocean by Yunhao Li, James K. B. Bishop, Phoebe J. Lam, Daniel C. Ohnemus

“…South of the APF, both satellite and birefringence sensor greatly overestimated PIC by factors of >25 and 12, respectively, compared to sample PIC which averaged 15 nM. The unanticipated discovery of a non‐carbonate particle birefringence source coupled with a microscopic investigation of pump samples leads us to hypothesize that internal reflection within bubbles and/or cellular structures of heavily silicified colony‐forming diatoms (Fragilariopsis and Pseudo‐nitzschia) is the cause for anomalous birefringence and adds to backscattered satellite radiances.…”
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Spatio-temporal dynamics of urbanization and environmental sustainability: A predictive modelling approach to forecasting land use transitions in Vellore, India by Sai Saraswathi Vijayaraghavalu, Kumaraguru Arumugam, Sakshi Dange

“…Future simulations using Cellular Automata and Artificial Neural Networks project that, by 2050, urban areas will constitute 27.1 %, whereas forest and agricultural land will decline further to 48.91 % and 9.15 %, respectively. …”
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Pharmacokinetics of Pegaspargase with a Limited Sampling Strategy for Asparaginase Activity Monitoring in Children with Acute Lymphoblastic Leukemia by Cristina Matteo, Antonella Colombini, Marta Cancelliere, Tommaso Ceruti, Ilaria Fuso Nerini, Luca Porcu, Massimo Zucchetti, Daniela Silvestri, Maria Grazia Valsecchi, Rosanna Parasole, Luciana Vinti, Nicoletta Bertorello, Daniela Onofrillo, Massimo Provenzi, Elena Chiocca, Luca Lo Nigro, Laura Rachele Bettini, Giacomo Gotti, Silvia Bungaro, Martin Schrappe, Paolo Ubezio, Carmelo Rizzari

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Non-invasive mapping of ventricular action potential waveforms reconstructed from clinical unshielded magnetocardiography. Potential diagnostic application and current limitations by Riccardo Fenici, Marco Picerni, Peter Fenici, Donatella Brisinda

“…Waveforms derived from 90-s averaged uMCG signals were comparable to those obtained with 300-s averages. …”
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Mortality impact, risks, and benefits of general population screening for ovarian cancer: the UKCTOCS randomised controlled trial by Usha Menon, Aleksandra Gentry-Maharaj, Matthew Burnell, Andy Ryan, Jatinderpal K Kalsi, Naveena Singh, Anne Dawnay, Lesley Fallowfield, Alistair J McGuire, Stuart Campbell, Steven J Skates, Mahesh Parmar, Ian J Jacobs

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