An immune-focused supplemental alignment pipeline captures information missed from dominant single-cell RNA-seq analyses, including allele-specific MHC-I regulation by Sebastian Benjamin, GW McElfresh, Maanasa Kaza, Gregory J. Boggy, Benjamin Varco-Merth, Benjamin Varco-Merth, Sohita Ojha, Shana Feltham, William Goodwin, William Goodwin, Candice Nkoy, Candice Nkoy, Derick Duell, Derick Duell, Andrea Selseth, Tyler Bennett, Aaron Barber-Axthelm, Nicole N. Haese, Nicole N. Haese, Helen Wu, Courtney Waytashek, Carla Boyle, Jeremy V. Smedley, Jeremy V. Smedley, Caralyn S. Labriola, Caralyn S. Labriola, Michael K. Axthelm, Michael K. Axthelm, R. Keith Reeves, R. Keith Reeves, Daniel N. Streblow, Daniel N. Streblow, Jonah B. Sacha, Jonah B. Sacha, Afam A. Okoye, Afam A. Okoye, Scott G. Hansen, Scott G. Hansen, Louis J. Picker, Louis J. Picker, Benjamin N. Bimber, Benjamin N. Bimber

“…These issues are frequently hidden or ignored, yet are highly relevant to immunologic data, where balancing selection has generated many polygenic gene families not accurately represented in a ‘one-size-fits-all’ reference genome.MethodsHere we present nimble, a tool to supplement standard RNA-seq pipelines. …”
Get full text

Abstract 221: EARLY EXPERIENCE OF EMBOGUARD BALLOON GUIDE CATHETER IN LARGE VESSEL OCCLUSION THROMBECTOMY by Bishow C. Mahat, Raul G. Nogueira, Mohamed Doheim, Priya Nidamanuri, Bharat Pillai, Michael J. Lang, Matthew T. Starr, Nirav R. Bhatt, Marcelo Rocha, Bradley Gross, Alhamza R. Al‐Bayati

“…Symptomatic intracranial hemorrhage was reported in 6 cases (10.5%), death at discharge was reported in 9 cases (15.8%), and access site complications were reported in 3 cases (5.3%). …”
Get full text

Race based disparities in clinical and financial outcomes associated with major elective and emergent surgery by Saad Mallick, MD, Sara Sakowitz, MS MPH, Syed Shahyan Bakhtiyar, MD MBE, Nam Yong Cho, BS, Troy Coaston, BS, Esteban Aguayo, MD, Peyman Benharash, MD

“…Methods: All adult hospitalizations for AAA, CABG, and COL were identified using the 2011–2020 National Inpatient Sample. …”
Get full text

The burden and care of orofacial cleft in Rwanda: a scoping review by Jimoh Amzat, Julienne Murererehe, Peace Uwambaye, Lauriyah Yusuf, Olusegun Olaopa, Afeez Salami, Ingabire Eliane Harerimana, Marie Claire Ineza, Eustache Ntigura, Jenipher Ober-Oluoch, Marie Aimee Dusenge, Dorcas Doree Nishimwe, Vestine Abimana, Gabriel Tuyishime, Danilo Zambrano Milanes, Akinyele Adisa, Ruwan Duminda Jayasinghe, Kehinde Kazeem Kanmodi

“…This scoping review aims to identify knowledge gaps, establish research agendas, and inform decision-making for policy redirection in Rwanda regarding OFCs. Methods The design and reportage of this scoping review were based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews. …”
Get full text

Long‐term efficacy (at and beyond 1 year) of gastric peroral endoscopic myotomy for refractory gastroparesis: A systematic review and meta‐analysis by Francesco Vito Mandarino, Alberto Barchi, Noemi Salmeri, Francesco Azzolini, Ernesto Fasulo, Giuseppe Dell'Anna, Edoardo Vespa, Emanuele Sinagra, Jeremie Jacques, Silvio Danese

“…Abstract Introduction Although gastric peroral endoscopic myotomy (G‐POEM) has shown substantial efficacy in patients with medically refractory gastroparesis (GP), comprehensive long‐term data on its effectiveness are lacking. Methods We conducted a systematic review and meta‐analysis including observational studies assessing long‐term efficacy after G‐POEM in patients with refractory GP. …”
Get full text

Carboxymethyl-lysine is a Prominent Target of Circulating IgA in IgA Nephropathy by Sara Alibrandi, Talita Aguiar, Mattea Ausmeier, Paolo Molinari, Enrico Fiaccadori, Umberto Maggiore, Nicholas Chun, Maria Lanau, Mario Perez-Arnedo, Joaquin Manrique, Paolo Cravedi, Emmanuel Zorn

“…We hypothesized that pathogenic IgA results from an immune response to abnormal protein modifications. Methods: We used an advanced enzyme-linked immunosorbent assay (ELISA) platform to assess serum IgA and Gd-IgA reactivity to 93 posttranslational modifications and other chemical adducts in 28 patients with biopsy-proven IgAN and 22 healthy controls. …”
Get full text

Measurement properties of health-related quality of life instruments for people living with scoliosis in China: A systematic review. by Liu-Qing Jing, Qi-Xing Shen, Yi-Lin Jin, Shao-Jie Du, Ting-Zhen Liu, Ni Chen, Guo-Dong Wang, Wen Mo, Jie Ye, Jin-Hai Xu

“…This systematic review examined the frequency of use of HR-QoL outcome measurement instruments among patients with scoliosis in China and synthesized evidence on their measurement properties.<h4>Methods</h4>Stage 1: We searched databases including MEDLINE, EMBASE, Scopus, PubMed, and the Web of Science from inception to December 2023 to identify studies that examined HR-QoL among patients with scoliosis in China. …”
Get full text

The Impact of physical activity and related factors on the risk of melanoma development and progression of the disease - A literature review by Joanna Szuba, Magdalena Rędaszka, Oliwia Barańska, Małgorzata Chańko, Justyna Drożdżał, Adam Krzaczkowski, Emilia Niedzieska, Zofia Szular, Tymon Wojczulis, Katarzyna Ziemek

“…It also examines sun-protection awareness, behaviors, and melanoma risk among outdoor athletes. Materials and methods In 2025, a literature review was conducted using PubMed, Web of Science, and Google Scholar, focusing on English-language studies analyzing the link between amateur or professional physical activity and melanoma. …”
Get full text

Diagnostic utility of single-locus DNA methylation mark in Sotos syndrome developed by nanopore sequencing-based episignature by Takeshi Mizuguchi, Nobuhiko Okamoto, Taiki Hara, Naoto Nishimura, Masamune Sakamoto, Li Fu, Yuri Uchiyama, Naomi Tsuchida, Kohei Hamanaka, Eriko Koshimizu, Atsushi Fujita, Kazuharu Misawa, Kazuhiko Nakabayashi, Satoko Miyatake, Naomichi Matsumoto

“…Among these differential DNA methylation sites, a single-locus DNA methylation mark at part of the NSD1 CpG island (CpGi) was subsequently studied in an additional 22 cases with a NSD1 point mutation or a 5q35 submicroscopic deletion involving NSD1. …”
Get full text

Meta Analysis of Methylenetetrahydrofolate Reductase (MTHFR) C677T polymorphism and its association with folate and colorectal cancer by Meng Ye, Guojie Xu, Liming Zhang, Zhihui Kong, Zhenhua Qiu

“…In this meta-analysis, the effects of MTHFR genotype and folate intake on colorectal cancer incidence were analyzed. Methods We searched PubMed,Embase, Web of Science, and CNKI database to identify relevant studies up to January 2024. …”
Get full text

Human IgM Antibodies to Malondialdehyde Conjugated With Albumin Are Negatively Associated With Cardiovascular Disease Among 60‐Year‐Olds by Divya Thiagarajan, Anna G. Frostegård, Sudhir Singh, Mizanur Rahman, Anquan Liu, Max Vikström, Karin Leander, Bruna Gigante, Mai‐Lis Hellenius, Bo Zhang, Roman A. Zubarev, Ulf de Faire, Susanna L. Lundström, Johan Frostegård

“…Methods and Results In a 5‐ to 7‐year follow‐up of 60‐year‐old men and women from Stockholm County previously screened for cardiovascular risk factors (2039 men, 2193 women), 209 incident CVD cases (defined as new events of coronary heart disease, fatal and nonfatal myocardial infarction, ischemic stroke, and hospitalization for angina pectoris) and 620 age‐ and sex‐matched controls were tested for IgM anti‐MDA by ELISA. …”
Get full text

Sleep and mental status as key factors to asthenopia in Chinese adults by Na Lin, Xin Chen, Xiao-Tian Wu, Fu-Yue Tian, Mao-Yuan Yang, Yong-Shun Liu, Fan Lyu, Ru-Zhi Deng

“…AIM: To investigate the occurrence of eye asthenopia in Chinese adults and pinpoint the factors contributing to it using a 17-item Asthenopia Survey Questionnaire (ASQ-17). METHODS: A total of 2509 Chinese adults aged 18 and above from 30 regions in China participated in a cross-sectional online survey in February 2020. …”
Get full text

From bench to bedside: elucidating VEGF(R) inhibitor-related heart failure in cancer treatment by Shengkun Peng, MinHong Cai, Hongyu Kuang, Anqi Lin, Qinghua Ma, Xiaoqin Dai, Peng Luo, Yijun Liu, Guo Zhang, Yifeng Bai

“…Abstract Background Vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) inhibitors play a pivotal role in treating various tumors; however, the clinical characteristics and molecular mechanisms of their associated heart failure (HF) remain incompletely understood. Methods We investigated the epidemiological characteristics of VEGF or VEGFR inhibitors [VEGF(R)i]-related heart failure (VirHF) using the global pharmacovigilance database Vigibase. …”
Get full text

Access and coverage of malaria services in Haiti in the context of elimination: a scoping review of the literature by Kenny Moise, Aude Melody Achille, Roubens Duvilaire, Jimmy Fedna, Roody Thermidor, Bertiny Bourdeau, Jessica Maureen Nestor, Gérald Lerebours, Jean Hugues Henrys, Christian Raccurt

“…Conclusion Research and reports on malaria surveillance, diagnosis, and treatment access and coverage in Haiti are limited; however, the available sources reveal significant shortcomings and challenges in implementing WHO-recommended policies and strategies for malaria elimination. Future studies should aim to fill this gap and investigate the determinants of the persistence of malaria cases in certain municipalities and departments of the country.…”
Get full text

Effect of acupoint catgut embedding for simple obesity in adults: A randomized controlled double-blind trial by Qiwang Yang, Nenggui Xu, Lidong Guo, Hui Li, Xiaoyan Li, Qingmei Zeng, Wenyue Zhang, Shiping Li, Wei Yi

“…Methods: In this randomized controlled, double-blind trial, 120 patients with simple obesity were initially allocated to verum ACE (n = 60) or sham ACE group (n = 60) .One participant allocated to the verum group was excluded during screening for failing to meet BMI criteria (<24 kg/m²), resulting in a final cohort of 59 verum and 60 sham cases completing the 12-week intervention and 12-week follow-up. …”
Get full text

Exploration of the role and mechanism of Rhizoma Paridis total saponins in osteosarcoma based on SPI1/LCN2-mediated ferroptosis by Ge Yang, Fenghui Li, Xiongke Hu, Miao Li, Yaoxi Liu, Guanghui Zhu, Qian Tan

“…BackgroundOsteosarcoma (OS) is a highly aggressive bone malignancy with limited therapeutic options and poor prognosis, particularly in cases of recurrence or metastasis. Recent studies have identified ferroptosis as a promising therapeutic target in OS, with the SPI1/LCN2 regulatory axis emerging as a critical modulator of this process. …”
Get full text

Prognostic Value of Trop‐2 Expression in Nonmetastatic Triple‐Negative Breast Cancer and Correlation With Emerging Biomarkers by William Jacot, Marie‐Christine Chateau, Simon Thezenas, Séverine Guiu, Nelly Firmin, Virginie Lafont, Gwendal Lazennec, Charles Theillet, Florence Boissière‐Michot

“…However, clinicopathological correlations with Trop‐2 protein expression levels remain limited in TNBC patients. Methods Here we assessed by immunohistochemistry (IHC) using the mouse monoclonal anti‐Trop‐2 antibody (Enzo, Cat. …”
Get full text

Transcatheter aortic valve implantation (TAVI) prostheses in vitro - biofilm formation and antibiotic effects by Torgny Sunnerhagen, Thomas Bjarnsholt, Klaus Qvortrup, Henning Bundgaard, Claus Moser

“…Background: Transcatheter aortic valve implantation (TAVI) is a percutaneous catheter-based treatment of aortic stenosis as an alternative to open heart valve surgery. In cases of TAVI endocarditis, the treatment possibilities may be limited as surgical removal of the infected valve may be associated with a high risk in elderly, comorbid or frail patients. …”
Get full text

The Long-Term Omalizumab Therapy in Children with Severe Persistent Uncontrolled Asthma: Evaluation of the Outcomes According to the Data of the Hospital Patient Registry by Elena A. Vishneva, Leyla S. Namazova-Baranova, Elena A. Dobrynina, Anna A. Alekseeva, Vladimir I. Smirnov, Julia G. Levina, Vera G. Kalugina, Kamilla E. Efendieva, Konstantin S. Volkov

“…Moreover, it allows conducting a continuous monitoring in a studied group. Objective: to analyze the effectiveness and safety of a long-term target therapy with Omalizumab (more than 4 years) in children with severe persistent uncontrolled asthma based on the electronic clinical cases database (registry). …”
Get full text

A machine learning model for predicting lymph node positivity in ovarian cancer: development, validation, and clinical application by QingYong Guo, Jinji Wang, Ru Chen, LiPing Hu, Wenqiang You

“…However, accurately predicting lymph node positivity remains challenging due to the disease’s heterogeneity and the limitations of traditional models in handling high-dimensional and imbalanced data.MethodsA retrospective analysis was conducted using the SEER database (2000–2021), including 26,844 OC patients with complete clinical information. …”
Get full text