Streamlined miRNA loading of surface protein-specific extracellular vesicle subpopulations through electroporation by Corinna Torabi, Sung-Eun Choi, Thomas R. Pisanic, Michael Paulaitis, Soojung Claire Hur

“…Conclusions Our streamlined method integrates magnetic bead-based selection with electroporation, enabling rapid and efficient loading of miRNA into CD81+ EVs. …”
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Conditional knockout of C/EBPβ in epidermis results in dysregulated lipid biosynthesis and a defect in skin barrier function. by Kevin J Mills, Michael Doyle, John S House, John G Witherspoon, Daniel Krakko, Whitney L Stutts, Jessie R Chappel, Jonathan R Hall, Erin S Baker, Robert C Smart

“…CCAAT/enhancer binding protein-β (C/EBPβ) is a basic leucine zipper transcription factor that is abundantly expressed in epidermal keratinocytes of skin. …”
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Research Progress on the Functional Regulation Mechanisms of ZKSCAN3 by Jianxiong Xu, Xinzhe Li, Jingjing Xia, Wenfang Li, Zhengding Su

“…Structurally, ZKSCAN3 is characterized by its conserved DNA-binding zinc finger motifs, a SCAN domain mediating protein–protein interaction, and a KRAB repression domain implicated in transcriptional regulation. …”
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miR-221 is a prognostic marker and promotes the proliferation and migration of esophageal squamous cell carcinoma by inhibiting autophagy by Kun Mei, Zilu Chen, Foxing Tan, YuHeng Zhou, Haolin Du, Renjun Gu, Yan Huang

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Proteomic signatures of human oral epithelial cells in HIV-infected subjects. by Elizabeth Yohannes, Santosh K Ghosh, Bin Jiang, Thomas S McCormick, Aaron Weinberg, Edward Hill, Faddy Faddoul, Mark R Chance

“…., heat-shock proteins, Cryab, Calr, IL-1RA, and Galectin-3-binding protein) as well as proteins involved in redox homeostasis and detoxification (e.g., Gstp1, Prdx1, and Ero1). …”
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m6A-related genes of peripheral white blood cell in spinal cord injury as potential targets for prognosis and treatment by Mingran Luo, Qian Wang, Jian Chen, Guoyong Yin

“…These genes are also involved in long-chain fatty acid binding. Early SCI was accompanied by significant immune cell infiltration. …”
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Exosomal circ_0093708 as a potential ferroptosis biomarker in cerebral ischemia–reperfusion injury by Shuyin Ma, Xiaodong Zhang, Jiaxin Fan, Mengying Chen, Qingling Yao, Nan Zhang, Kaili Shi, Shuang Du, Yuxuan Cheng, Huiyang Qu, Minyu Duan, Han Yang, Tiantian Gao, Shuqin Zhan

“…This study aims to identify key exosomal markers of ferroptosis.MethodsBy integrating and analyzing multiple GSE datasets, we identified ferroptosis-associated key genes. …”
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Inhibitory effects of four different kinds of triazole fungicides against Fusarium oxysporum f. sp. cubense and their differences by XIANG Dandan, YANG Xiaofang, YI Ganjun, TAO Haiqing, CHU Yuanqi, LI Chunyu

“…The morphology changes and membrane integrity of Foc TR4 in response to these four fungicides were evaluated by morphological observation of hyphae, and detection of relative conductivity and malondialdehyde (MDA) concentrations. …”
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Deciphering the role of molecular mimicry in the etiopathogenesis of Autoimmune Hemolytic Anemia using an immunoinformatics approach. by Pratyusha Patidar, Arihant Jain, Tulika Prakash

“…HLA DRB1_0305 was found to exhibit binding with several bacterial epitopes indicating its predisposing potential to AIHA. …”
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Computational screening identifies selective aldose reductase inhibitors with strong efficacy and limited off target interactions by Jicli Jose Rojas, Roberto Pestana-Nobles, Leonardo C. Pacheco-Londono, Jesús Utria-Munive, Nataly J. Galan-Freyle

“…Because existing AR inhibitors often cause off-target toxicity, we implemented an integrative in-silico workflow to discover selective, safer compounds. …”
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Intrinsic Disorder and Phase Separation Coordinate Exocytosis, Motility, and Chromatin Remodeling in the Human Acrosomal Proteome by Shivam Shukla, Sean S. Lastorka, Vladimir N. Uversky

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Effect of docetaxel administration on fluid dynamics in mice by Ayana Mawaki, Masushi Kohta, Aya Yoshimura, Toshio Nakatani, Shizuko Nagao, Junko Sugama

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Legitimacy as Social Infrastructure: A Critical Interpretive Synthesis of the Literature on Legitimacy in Health and Technology by Sydney Howe, Carin Uyl-de Groot, Rik Wehrens

“… BackgroundAs technology is integrated into health care delivery, research on adoption and acceptance of health technologies leaves large gaps in practice and provides limited explanation of how and why certain technologies are adopted and others are not. …”
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Development of immune-derived molecular markers for preeclampsia based on multiple machine learning algorithms by Zhichao Wang, Long Cheng, Guanghui Li, Huiyan Cheng

“…This study presents a robust prediction model for PE that integrates multiple machine learning–derived immune-related biomarkers. …”
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Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development. by Gunnar R Mair, Edwin Lasonder, Lindsey S Garver, Blandine M D Franke-Fayard, Céline K Carret, Joop C A G Wiegant, Roeland W Dirks, George Dimopoulos, Chris J Janse, Andrew P Waters

“…Containing translationally silent transcripts, this mRNP integrates eIF4E and poly(A)-binding protein but excludes P body RNA degradation factors and translation-initiation promoting eIF4G. …”
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A role for fibrillar collagen deposition and the collagen internalization receptor endo180 in glioma invasion. by Ivo J Huijbers, Marjan Iravani, Sergey Popov, David Robertson, Safa Al-Sarraj, Chris Jones, Clare M Isacke

“…Correlated with this collagen deposition we observed high level expression of the collagen-binding receptor Endo180 (CD280) in the tumor cells. …”
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NuclampFISH enables cell sorting based on nuclear RNA expression for chromatin analysis by Yifang Liu, Yuchen Qiu, Keqing Nian, Sara H. Rouhanifard

“…Additionally, the required formaldehyde fixation interferes with downstream analysis of chromatin and protein-binding interactions. Results To address these challenges, we developed a platform, nuclear clampFISH (nuclampFISH) that integrates click-amplified FISH with reversible crosslinkers and allows access to the nucleus. …”
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Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co₃O₄ Nanocatalyst by Changfeng Zhu, Yujiao Zhao, Jiyang Liu

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Charting γ-secretase substrates by explainable AI by Stephan Breimann, Frits Kamp, Gabriele Basset, Claudia Abou-Ajram, Gökhan Güner, Kanta Yanagida, Masayasu Okochi, Stephan A. Müller, Stefan F. Lichtenthaler, Dieter Langosch, Dmitrij Frishman, Harald Steiner

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Seashell-based bioceramics for advanced electrospun tissue scaffolds by Sema Nur Sahin, Erdi Bulus, Alper Tezcan, Muhammad Umar Farooq, Marwah Al-garash, Yesim Muge Sahin

“…This study focuses on developing tissue scaffolds with enhanced cell adhesion, biodegradability, and tensile strength by employing aqueous solutions of polyvinyl alcohol (PVA), a biocompatible and biodegradable synthetic polymer; gelatin (GEL), a natural polymer that offers binding sites conducive to cell adhesion and differentiation; and synthesized bioceramics, all integrated through the electrospinning process. …”
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